BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0160.p (221 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 381 e-107 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 219 1e-58 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 175 2e-45 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 131 3e-32 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 124 8e-30 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 381 bits (978), Expect = e-107, Method: Compositional matrix adjust. Identities = 188/193 (97%), Positives = 190/193 (98%) Query: 11 AKQRATIIKRWNDLLLAHQEDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRANG 70 AKQRA IIKRWNDLLLAHQ+DLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRANG Sbjct: 72 AKQRAGIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRANG 131 Query: 71 DIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTPLT 130 DIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTPLT Sbjct: 132 DIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTPLT 191 Query: 131 SLALIRLALEAGVPAGVINIVTASRERTPEVVDVWLADGRVRKISFTGSTAVGKHLARHS 190 SLAL+RLA EAGVP GVINIVTASRERTPEVVDVWLADGRVRKISFTGSTAVGKHLARHS Sbjct: 192 SLALVRLAQEAGVPVGVINIVTASRERTPEVVDVWLADGRVRKISFTGSTAVGKHLARHS 251 Query: 191 ADTLKKLSLELGG 203 ADTLKKLSLELGG Sbjct: 252 ADTLKKLSLELGG 264 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 219 bits (558), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 111/194 (57%), Positives = 145/194 (74%), Gaps = 5/194 (2%) Query: 11 AKQRATIIKRWNDLLLAHQEDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRANG 70 AK+R+ I++RW +L+LA+ +DL +++ EQGKPLAEA+GE+ YAAS++EWF EE R G Sbjct: 70 AKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYG 129 Query: 71 DIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTPLT 130 D IP+P R++ +KE +GV AAITPWNFPAAMI RK PALAAGC +V KPAE TP + Sbjct: 130 DTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFS 189 Query: 131 SLALIRLALEAGVPAGVINIVTASRERTPEVVDVWL-ADGRVRKISFTGSTAVGKHLARH 189 +LAL LA AG+PAGV ++VT TP+ + + ++ VRKISFTGST VGK L + Sbjct: 190 ALALAVLAERAGIPAGVFSVVTG----TPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQ 245 Query: 190 SADTLKKLSLELGG 203 SA ++KKLSLELGG Sbjct: 246 SASSIKKLSLELGG 259 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 175 bits (444), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 93/193 (48%), Positives = 126/193 (65%), Gaps = 3/193 (1%) Query: 11 AKQRATIIKRWNDLLLAHQEDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRANG 70 A +R+ I++R DLL E++ RL++ EQGKPLAEAK E AA ++WF EEA R G Sbjct: 63 AFERSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYG 122 Query: 71 DIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTPLT 130 ++PA V G M +KEPVG VAA TPWNFP + RK++ ALAAGC+++ K E+TP + Sbjct: 123 RLVPARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPAS 182 Query: 131 SLALIRLALEAGVPAGVINIVTASRERTPEVVDVWLADGRVRKISFTGSTAVGKHLARHS 190 LIR +AGVPAGVIN+V E+ + + ++KISFTGST VGK LA + Sbjct: 183 PAELIRAYADAGVPAGVINLVYGV---PAEISEYLIPHPVIKKISFTGSTPVGKQLAALA 239 Query: 191 ADTLKKLSLELGG 203 +K+ ++ELGG Sbjct: 240 GQHMKRATMELGG 252 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 131 bits (330), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 76/193 (39%), Positives = 108/193 (55%), Gaps = 4/193 (2%) Query: 11 AKQRATIIKRWNDLLLAHQEDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRANG 70 A +R+ ++ + L HQE+L +L R+ GKP+ +A+ + A Y E++ A + +G Sbjct: 61 AAERSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHG 120 Query: 71 DIIPAPVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAPALAAGCTVVCKPAEDTPLT 130 D IP G ++ L+EP GV I PWN+P + R + ALAAG T V KPAED L+ Sbjct: 121 DTIPYQ-AGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLS 179 Query: 131 SLALIRLALEAGVPAGVINIVTASRERTPEVVDVWLADGRVRKISFTGSTAVGKHLARHS 190 L + LA E G P G +NIVT E D+ + V ISFTGS VGK + R + Sbjct: 180 ILRVAELAAEVGFPEGALNIVTGYGH---EAGDLLVKHPDVNHISFTGSPNVGKLVVRAA 236 Query: 191 ADTLKKLSLELGG 203 A+ ++LELGG Sbjct: 237 AEHHTPVTLELGG 249 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 124 bits (310), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 75/169 (44%), Positives = 107/169 (63%), Gaps = 5/169 (2%) Query: 36 ISREQGKPLAEAKGEVAYAASYVEWFGEEATRANGDIIPAPVTGRRMMALKEPVGVVAAI 95 I+ + GKPLA+A E+ AA +++W EE R G ++ A R + ++EPVGV AAI Sbjct: 88 ITMDNGKPLADAVAEIINAAEHLDWHAEEGRRLYGRVVGARHPEVRQLVVREPVGVCAAI 147 Query: 96 TPWNFPAAMIARKIAPALAAGCTVVCKPAEDTPLTSLALIRLALEAGVPAGVINIVTA-S 154 +PWNFP +K+A A+AAGCTVV K ++P + + +L +AGVPAGV NIV+ S Sbjct: 148 SPWNFPFGQAMKKVAAAMAAGCTVVLKGPSESPAAIVLMAQLFHDAGVPAGVFNIVSGVS 207 Query: 155 RERTPEVVDVWLADGRVRKISFTGSTAVGKHLARHSADTLKKLSLELGG 203 E + +++ L VR ISFTGS VG LA +A +K++S+ELGG Sbjct: 208 SEISQHLIESPL----VRNISFTGSVPVGSKLAAQAALHMKRMSMELGG 252 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 689,785 Number of extensions: 29981 Number of successful extensions: 179 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 157 Number of HSP's successfully gapped: 19 Length of query: 221 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 139 Effective length of database: 1,231,600 Effective search space: 171192400 Effective search space used: 171192400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)