BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0159.p (198 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 387 e-109 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 221 3e-59 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 145 2e-36 gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrog... 144 4e-36 gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 122 2e-29 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 387 bits (993), Expect = e-109, Method: Compositional matrix adjust. Identities = 187/198 (94%), Positives = 192/198 (96%) Query: 1 VSPNRIYVQDKVYETFVAKLGARVAALKVGPATDPASQIGPMINARAIGKIDQHVRDAIE 60 VSPNRIYVQ+KVY+ FV KLGARVAALKVGPATDPASQIGPMINARAI KIDQHVRDAI Sbjct: 296 VSPNRIYVQEKVYDAFVDKLGARVAALKVGPATDPASQIGPMINARAIAKIDQHVRDAIA 355 Query: 61 RGARVITGGKRLQGPGFESDNYYAPTVLANVTGEMQCSCEETFGPVAPITRFSTEEEVIA 120 RGARVITGGKRLQGPGF SDNYYAPTVLA+VTG MQCSCEETFGPVAPITRF+TE+EVIA Sbjct: 356 RGARVITGGKRLQGPGFGSDNYYAPTVLADVTGAMQCSCEETFGPVAPITRFATEDEVIA 415 Query: 121 AANATPFGLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGS 180 AANATPFGLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGS Sbjct: 416 AANATPFGLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGS 475 Query: 181 VHGLDDYLHTKYVCQGQL 198 VHGLDDYLHTKYVCQGQL Sbjct: 476 VHGLDDYLHTKYVCQGQL 493 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 221 bits (563), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 110/196 (56%), Positives = 140/196 (71%), Gaps = 4/196 (2%) Query: 1 VSPNRIYVQDKVYETFVAKLGARVAALKVGPATDPASQIGPMINARAIGKIDQHVRDAIE 60 V NRIYVQD VY+ F AKL V KVG T+ GP+IN +A+ K++QHV DA+ Sbjct: 291 VCANRIYVQDGVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVA 350 Query: 61 RGARVITGGKRLQGPGFESDNYYAPTVLANVTGEMQCSCEETFGPVAPITRFSTEEEVIA 120 +GARV+ GGKR +++ PTVLA+VT MQ + EETFGP+AP+ RF T+EEV+A Sbjct: 351 KGARVLLGGKRHA----LGHSFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLA 406 Query: 121 AANATPFGLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGS 180 AN T FGLA+YFYS D+ RI RV + LESG+VG+N G ++ E APFGGVK+SG GREGS Sbjct: 407 LANDTEFGLASYFYSRDIGRIWRVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGREGS 466 Query: 181 VHGLDDYLHTKYVCQG 196 +G+DDYL KY+C G Sbjct: 467 HYGIDDYLVVKYLCMG 482 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 145 bits (366), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 77/195 (39%), Positives = 111/195 (56%), Gaps = 4/195 (2%) Query: 1 VSPNRIYVQDKVYETFVAKLGARVAALKVGPATDPASQIGPMINARAIGKIDQHVRDAIE 60 VSP R VQ V+ FV K LKVG + +GP+ N R I ++ + DA+ Sbjct: 284 VSPTRFLVQKGVFNAFVDKFVTYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVS 343 Query: 61 RGARVITGGKRLQGPGFESDNYYAPTVLANVTGEMQCSCEETFGPVAPITRFSTEEEVIA 120 +GA++ TGGKR+ G+ ++ PTVL N+ + + EE FGP+A I F T +EVI Sbjct: 344 QGAQLRTGGKRIGNKGY----FFQPTVLTNLPLQARAMNEEPFGPLALINSFETLDEVIT 399 Query: 121 AANATPFGLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGS 180 AN P+GLAAY ++ H + +ESG++ +N LA PFGG+K+SGYG EG Sbjct: 400 EANRLPYGLAAYAFAKSAHTKHALAARVESGMLTINHLGLALPELPFGGIKDSGYGSEGG 459 Query: 181 VHGLDDYLHTKYVCQ 195 ++ Y++TK V Q Sbjct: 460 TEAIEAYINTKLVTQ 474 >gi|300313231|ref|YP_003777323.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 480 Score = 144 bits (364), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 79/195 (40%), Positives = 116/195 (59%), Gaps = 4/195 (2%) Query: 1 VSPNRIYVQDKVYETFVAKLGARVAALKVGPATDPASQIGPMINARAIGKIDQHVRDAIE 60 VSP+R YVQ +Y FV + +KVGP +P Q+GP+I+ R + + DA+ Sbjct: 284 VSPSRFYVQKGIYARFVTAFAEALLQIKVGPGHEPGVQMGPLIHGRRFEAVQGLIDDALA 343 Query: 61 RGARVITGGKRLQGPGFESDNYYAPTVLANVTGEMQCSCEETFGPVAPITRFSTEEEVIA 120 +G+ ++TGG RL G ++YAPT++ V + EE FGP+A +T FS EE IA Sbjct: 344 QGSELVTGGARLGQRG----HFYAPTLITGVPPSARVMQEEPFGPIAAVTPFSQAEEAIA 399 Query: 121 AANATPFGLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGS 180 ANA P GLA+Y ++ + HRV+ L++G+V +N +A PFGG+ ESG+G EG Sbjct: 400 MANALPLGLASYVFTQSLDTAHRVSRDLQAGMVNINHSGMAHPELPFGGIGESGFGSEGG 459 Query: 181 VHGLDDYLHTKYVCQ 195 V G + +L TK + Q Sbjct: 460 VEGFESFLVTKMISQ 474 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 122 bits (306), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 71/194 (36%), Positives = 103/194 (53%), Gaps = 4/194 (2%) Query: 1 VSPNRIYVQDKVYETFVAKLGARVAALKVGPATDPASQIGPMINARAIGKIDQHVRDAIE 60 V+ +R+ VQ KVY+ FV + A +++G D ++++GP+ N + I + V +E Sbjct: 300 VAGSRLLVQRKVYDRFVETVAAGAEKIRLGAPLDDSTEVGPINNRKQYEHIQRMVARGLE 359 Query: 61 RGARVITGGKRLQGPGFESDNYYAPTVLANVTGEMQCSCEETFGPVAPITRFSTEEEVIA 120 GA + G R G+ + PT+LA+ + M+ + E FGPVA F EEE IA Sbjct: 360 AGATLAAGHTRYGEEGY----FVRPTLLAHASNAMEVARSEIFGPVAVAIPFEDEEEAIA 415 Query: 121 AANATPFGLAAYFYSTDVRRIHRVTDALESGIVGVNEGALAAEAAPFGGVKESGYGREGS 180 AN + FGLA ++ DV R HRV ++ +G VN A+PFGG SGYGR Sbjct: 416 IANDSEFGLAGAVWTRDVARAHRVAASVNAGTFWVNSYKTINVASPFGGFNRSGYGRSSG 475 Query: 181 VHGLDDYLHTKYVC 194 + L DY TK V Sbjct: 476 MEALYDYTQTKSVW 489 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 663,075 Number of extensions: 28247 Number of successful extensions: 127 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 113 Number of HSP's successfully gapped: 18 Length of query: 198 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 118 Effective length of database: 1,241,070 Effective search space: 146446260 Effective search space used: 146446260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)