BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0157.p (461 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310456|ref|YP_003774548.1| family II aminotransferase [Her... 895 0.0 gi|300312816|ref|YP_003776908.1| aminotransferase class III prot... 221 1e-58 gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate amino... 202 4e-53 gi|300311765|ref|YP_003775857.1| adenosylmethionine-8-amino-7-ox... 181 1e-46 gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferas... 140 2e-34 >gi|300310456|ref|YP_003774548.1| family II aminotransferase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 895 bits (2314), Expect = 0.0, Method: Compositional matrix adjust. Identities = 438/461 (95%), Positives = 448/461 (97%), Gaps = 3/461 (0%) Query: 1 MQTSALAALDRSHLIHPVASFREHEELGPIILKSGHGAYLTDHQDKQLLDAFSGLWCVNT 60 MQTSALAALDR HLIHPV S REHEELGP+ILKSG GAYLTDHQDKQLLDAFSGLWCVNT Sbjct: 1 MQTSALAALDRRHLIHPVTSLREHEELGPLILKSGQGAYLTDHQDKQLLDAFSGLWCVNT 60 Query: 61 GYGQESVIRAATEQMQRLPYATGYFHFASEPAILLAKKLVDISPASLKHVYFTLGGSDAV 120 GYGQESVIRAATEQMQRLPYATGYFHFASEPAILLAKKLVDISPASL+HVYFTLGGSDAV Sbjct: 61 GYGQESVIRAATEQMQRLPYATGYFHFASEPAILLAKKLVDISPASLQHVYFTLGGSDAV 120 Query: 121 DAAIRYITHYHNSIGKPTKKHFISLQRGYHGSSSVGAGLTALPNFHYNFNLPLPTQHYLP 180 DAAIRYITHYH+SIGKP K+HFISLQRGYHGSSSVGAGLTALPNFHY+FNLPLPTQHYLP Sbjct: 121 DAAIRYITHYHHSIGKPGKQHFISLQRGYHGSSSVGAGLTALPNFHYHFNLPLPTQHYLP 180 Query: 181 SPYPYRSELQGDEAIIAASVQSLRDKVAELGADNVAAFFCEPIQGSGGVVVPPKGWLKAM 240 SPYPYRSELQGDEAIIAASVQ+LRDKVAELGADNVAAFFCEPIQGSGGVVVPPKGWLKAM Sbjct: 181 SPYPYRSELQGDEAIIAASVQALRDKVAELGADNVAAFFCEPIQGSGGVVVPPKGWLKAM 240 Query: 241 QLACNQLDILFVVDEVITGFGRTGPMFACLDEDVQPDLMTMAKGLTAGXGGTRTMGAVMM 300 QLACN+LDILFVVDEVITGFGRTGPMFACLDEDVQPDLMTMAKGLTAG MGAVMM Sbjct: 241 QLACNELDILFVVDEVITGFGRTGPMFACLDEDVQPDLMTMAKGLTAGYA---PMGAVMM 297 Query: 301 SDRVYNGIADGAPMSTVVGHGQTYSAHPVSAAVALEVLRLYEEGGLLENGRRLEPVFAGG 360 SDRVYNGIADGAPMSTVVGHGQTYSAHPVSAAVALEVLRLYEEGGLL+NGRRLEPVFAGG Sbjct: 298 SDRVYNGIADGAPMSTVVGHGQTYSAHPVSAAVALEVLRLYEEGGLLDNGRRLEPVFAGG 357 Query: 361 LRALLDHPLVGDARSRGLLGALELVADKQTKARFAPELKLHDRIFQVAYRHGLVFRAFGD 420 LRALL HPLVGDARSRGLLGALELVADKQTKARFAPELKLHDRIFQ AYRHGLVFRAFGD Sbjct: 358 LRALLAHPLVGDARSRGLLGALELVADKQTKARFAPELKLHDRIFQAAYRHGLVFRAFGD 417 Query: 421 NILGFAPALCYTEEDFALMFARLRAILDEVLAQPEVRQAVR 461 NILGFAPALCYT+EDFALMFARL+AILDEVLAQPEVRQAVR Sbjct: 418 NILGFAPALCYTQEDFALMFARLKAILDEVLAQPEVRQAVR 458 >gi|300312816|ref|YP_003776908.1| aminotransferase class III protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 221 bits (563), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 144/422 (34%), Positives = 222/422 (52%), Gaps = 20/422 (4%) Query: 34 SGHGAYLTDHQDKQLLDAFSGLWCVNTGYGQESVIRAATEQMQRLPYATGYFHFASEPAI 93 +G G L D K+ +DA G G+G VI A +Q+ L YA F F + PA Sbjct: 18 AGEGMELIDQDGKRYIDASGGAAVSCLGHGHPRVIEAIRKQVGELAYAHTSF-FTTAPAE 76 Query: 94 LLAKKLVDISPASLKHVYFTLGGSDAVDAAIRYITHYHNSIGKPTKKHFISLQRGYHGSS 153 LA L D +P SL HVYF GGS+AV+AA++ Y+ +G+P ++H I+ ++ YHG + Sbjct: 77 ELAAMLADAAPGSLNHVYFLSGGSEAVEAALKLARQYYVEVGQPQRRHIIARRQSYHG-N 135 Query: 154 SVGAGLTALPNFHYNFNLPLPTQ-HYLPSPYPYRSELQGDEAI--IAASVQSLRDKVAEL 210 ++GA + +P+ + H++ Y YR+ G+ + + L K+ L Sbjct: 136 TLGALAIGGNAWRREMFMPMLIEAHHVSPCYAYRNRADGESDVQYVQRLADELEQKILSL 195 Query: 211 GADNVAAFFCEPIQGSGGVVVPPKG-WLKAMQLACNQLDILFVVDEVITGFGRTGPMFAC 269 GAD V AF E + G+ VPP G + + ++ C++ +L ++DEV++G GRTG +FAC Sbjct: 196 GADQVIAFVAETVVGATAGAVPPVGDYFRKIRAVCDKYGVLLILDEVMSGMGRTGYLFAC 255 Query: 270 LDEDVQPDLMTMAKGLTAGXGGTRTMGAVMMSDRVYNGIADGAPMSTVVGHGQTYSAHPV 329 ++ V PD++ +AKGL AG + +GA++ SD +Y+ + G S HG TY H Sbjct: 256 EEDGVVPDIVVIAKGLGAGY---QPIGAMICSDHIYDAVLRG---SGFFQHGHTYIGHAT 309 Query: 330 SAAVALEVLRLYEEGGLLENGRRLEPVFAGGLR-ALLDHPLVGDARSRGLLGALELVADK 388 + A A+ V + +E LLEN R+ LR A D VGD R RGL +ELVA++ Sbjct: 310 ACAAAVAVQKTIQEERLLENVRQRGEQLRSELRQAFGDQAHVGDIRGRGLFVGVELVAER 369 Query: 389 QTKARFAPELKLHDRIFQVAYRHGLVFRAFGDNILG-------FAPALCYTEEDFALMFA 441 +K +P+L+ H R+ A + GL+ G I G AP + D + + Sbjct: 370 SSKLPLSPDLRTHARVKAEAMKRGLLVYPMGGTIDGKNGDHILLAPPFIASSNDISEIVQ 429 Query: 442 RL 443 RL Sbjct: 430 RL 431 >gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 442 Score = 202 bits (514), Expect = 4e-53, Method: Compositional matrix adjust. Identities = 140/423 (33%), Positives = 208/423 (49%), Gaps = 17/423 (4%) Query: 29 PIILKSGHGAYLTDHQDKQLLDAFSGLWCVNTGYGQESVIRAATEQMQRLPYATGYFHFA 88 P +L S G + D +LD +GLWCV G+ ++ A + + L YA F Sbjct: 29 PRMLVSAEGMHYRDDAGNTILDGTAGLWCVPLGHAHPKIVSAVQKSVATLDYAPS-FQLG 87 Query: 89 SEPAILLAKKLVDISPASLKHVYFTLGGSDAVDAAIRYITHYHNSIGKPTKKHFISLQRG 148 A LA++L + +V++T GS+AVD A++ YH + G T+ I +RG Sbjct: 88 HPSAFELAERLKAYTGNRFGNVFYTGSGSEAVDTALKIALAYHRAKGNATRTRLIGRERG 147 Query: 149 YHGSSSVGAGLTALPNFHYNFNLPL-PTQHYLPSPYPYRSE--LQGDEAIIAASVQSLRD 205 YHG G + + F+ L P +LP + +G+ A L Sbjct: 148 YHGVGFGGMSVGGIGGNRKTFSTALLPGVDHLPHTHNLEKNAFTKGEPEYGAHLADELER 207 Query: 206 KVAELGADNVAAFFCEPIQGSGGVVVPPKGWLKAMQLACNQLDILFVVDEVITGFGRTGP 265 V A N+AA EP+ GS GV++PPKG+LK ++ C + IL + DEVITGFGR G Sbjct: 208 LVTLHDASNIAAVIVEPVAGSTGVLIPPKGYLKRLREICTKHGILLIFDEVITGFGRLGT 267 Query: 266 MFACLDEDVQPDLMTMAKGLTAGXGGTRTMGAVMMSDRVYNGIADGAPMSTVVGHGQTYS 325 FA DV+PD+ T AKGLT G MGAV + +++ +G P + HG TYS Sbjct: 268 PFASDYFDVEPDIFTTAKGLT---NGVVPMGAVFVKPEIHDAFMNG-PDGIELFHGYTYS 323 Query: 326 AHPVSAAVALEVLRLYEEGGLLENGRRLEPVFAGGLRALLDHPLVGDARSRGLLGALELV 385 HP++ A L L +++ G+LEN + + F AL P V D R+ GL+ +EL Sbjct: 324 GHPLACAAGLASLEVFQTEGVLENAQAVADYFQEAAHALRGLPHVIDVRTIGLIAGIELA 383 Query: 386 A-DKQTKARFAPELKLHDRIFQVAYRHGLVFRAFGDNILGFAPALCYTEEDFALMFARLR 444 + + AR +D F A+ G++ R GD I+ +P L ++ +F +L Sbjct: 384 SIPGRVGAR------AYD-AFVRAFNDGILIRVTGD-IIALSPPLIINKQQIDELFGKLA 435 Query: 445 AIL 447 AIL Sbjct: 436 AIL 438 >gi|300311765|ref|YP_003775857.1| adenosylmethionine-8-amino-7-oxononanoate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 448 Score = 181 bits (458), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 120/435 (27%), Positives = 201/435 (46%), Gaps = 14/435 (3%) Query: 3 TSALAALDRSHLIHPVASFREHEELGPIILKSGHGAYLTDHQDKQLLDAFSGLWCVNTGY 62 +SAL A + HP R HE PI + HG +L D ++ LDA S W G+ Sbjct: 6 SSALVARSLRSVWHPCTQMRRHESAPPIAITRAHGLWLHDSDGRRYLDAISSWWVNLFGH 65 Query: 63 GQESVIRAATEQMQRLPYA--TGYFHFASEPAILLAKKLVDISPASLKHVYFTLGGSDAV 120 + A EQ+ +L +A G+ H EP + L+++L ++ +L H ++ G+ AV Sbjct: 66 TNTRINAALVEQLGQLEHAMLAGFTH---EPVVALSEQLAALTGQALGHCFYASDGASAV 122 Query: 121 DAAIRYITHYHNSIGKPTKKHFISLQRGYHGSSSVGAGLTALPNFHYNFNLPLPTQHYLP 180 + A++ H + G+P K FI + GYHG + G+T P F + L H Sbjct: 123 EIALKMSVHAWRNGGQPDKNEFICIAGGYHGETVGALGVTDTPLFRSAYESLLRPAHVAA 182 Query: 181 SPYPYRSELQGDEAIIAASVQSLRDKVAELGADNVAAFFCEP-IQGSGGVVVPPKGWLKA 239 SP ++ +AA +++ + +AA EP +Q + G+ + G+++ Sbjct: 183 SPDARQAAPGESAEEVAARALDDVERILQQRQGRIAALIVEPLVQCAAGMAMHDPGYVRG 242 Query: 240 MQLACNQLDILFVVDEVITGFGRTGPMFACLDEDVQPDLMTMAKGLTAGXGGTRTMGAVM 299 ++ C++ + DE+ G GRTG FAC V PDL+ ++KG++ GG + VM Sbjct: 243 LRSLCDRYGAHLIADEIAVGCGRTGSFFACEQAGVWPDLLCLSKGIS---GGYLPLSLVM 299 Query: 300 MSDRVYNGIADGAPMSTVVGHGQTYSAHPVSAAVALEVLRLYEEGGLLENGRRLEPVFAG 359 S+ +Y D + H +Y+ +P++ AL L ++ E +LE R PV A Sbjct: 300 SSEAIYQAFYDDDVRRGFL-HSHSYTGNPLACRAALATLAIFAEDAVLERNRAFAPVLAA 358 Query: 360 GLRALLDHPLVGDARSRGLLGALELVADKQTKARFAPELKLHDRIFQVAYRHGLVFRAFG 419 L+ L P V R RG++ A + V + A A R+ A + ++ R G Sbjct: 359 ALQPLAQDPRVRHVRQRGMIWAYDAVLEGSAAATTA----FSRRMAAEAAQREVLVRPIG 414 Query: 420 DNILGFAPALCYTEE 434 ++ P L E+ Sbjct: 415 ASLYLMPPYLLDEEQ 429 >gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 456 Score = 140 bits (354), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 123/426 (28%), Positives = 195/426 (45%), Gaps = 38/426 (8%) Query: 29 PIILKSGHGAYLTDHQDKQLLDAFSGLWCVNTGYGQESVIRAATEQMQRLPYATGYFHFA 88 PI + G D +D +G+ + G V+ A +Q + L ++ Sbjct: 48 PIAMDQAFGVTFKDPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLMHS---MEVN 104 Query: 89 SEPAILLAKKLVDISPASLKH---VYFTLGGSDAVDAAIRYITHYHNSIGKPTKKH-FIS 144 S LA K+ +I P L+ +FT GGSDA++AA+++ T +H I+ Sbjct: 105 SSKRTELAAKISEIMPDGLRGDCITFFTQGGSDALEAAVKFAKRV-------TGRHQIIA 157 Query: 145 LQRGYHGSSSVGAGLTALPNFHYNFNLPLPTQHYLPSPYPYR----SELQGDEAIIAASV 200 GYHG + LT + F + + P PY YR + + E I V Sbjct: 158 FHGGYHGIWNASNALTTGTAYRKGFGPFMGGVIHAPYPYAYRFPFDTSHKSAEQIAGEYV 217 Query: 201 QSLRDKVAELGADNVAAFFCEPIQGSGGVVVPPKGWLKAMQLACNQLDILFVVDEVITGF 260 L + AD+VAA EP+QG GG V P +L+ ++ AC++ L +VDEV G Sbjct: 218 DYLLN-TPYTAADDVAAVIVEPVQGEGGYVPPSPEFLQILRKACDRSGALLIVDEVQAGA 276 Query: 261 GRTGPMFACLDEDVQPDLMTMAKGLTAGXGGTRTMGAVMMSDRVYNGIADGAPMSTVVGH 320 GRTG M+A V+PD++T KG+ GG M ++M + I DG+ Sbjct: 277 GRTGKMWAVEHSGVKPDMLTFGKGI----GGDMPMAGLVMRSDLAAKIPDGS-------Q 325 Query: 321 GQTYSAHPVSAAVALEVLRLYEEGGL-LENGRRLEPVFAGGLRALLDHPLVGDARSRGLL 379 T++A+ +SAAVAL + + ++ L L N + A + P VG+ R RGL+ Sbjct: 326 PNTFAANSISAAVALTNISILQDPRLDLVNRAHTLGLEAQERIRSFNSPWVGEVRGRGLM 385 Query: 380 GALELVADKQTKARFAPELKLHDRIFQVAYRHGLVFRAFG--DNILGFAPALCYTEEDFA 437 +ELV +++T+ + E KL ++ HG++ G N++ P+L + Sbjct: 386 IGIELVENRETREPLSRE-KL-GKLMDYVVGHGVLMIPCGRYTNVMRVMPSLTIPR---S 440 Query: 438 LMFARL 443 LMF L Sbjct: 441 LMFKGL 446 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,494,476 Number of extensions: 65195 Number of successful extensions: 217 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 194 Number of HSP's successfully gapped: 9 Length of query: 461 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 373 Effective length of database: 1,203,190 Effective search space: 448789870 Effective search space used: 448789870 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)