BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0145.p (830 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310446|ref|YP_003774538.1| sulfite reductase, flavoprotein... 1288 0.0 gi|300311804|ref|YP_003775896.1| iron-regulated membrane protein... 72 1e-13 gi|300311746|ref|YP_003775838.1| iron-regulated membrane protein... 65 3e-11 >gi|300310446|ref|YP_003774538.1| sulfite reductase, flavoprotein component [Herbaspirillum seropedicae SmR1] Length = 850 Score = 1288 bits (3333), Expect = 0.0, Method: Compositional matrix adjust. Identities = 656/853 (76%), Positives = 716/853 (83%), Gaps = 26/853 (3%) Query: 1 MFKKIWFQLHWFIGITAGTLLMTIGVTGAVLAFREEIMEALSPGVMHVQVRQQATLTPQQ 60 MFKKIWFQLHWFIGITAGT+LMTIGVTGA+LAFREEIM+ LSPGVMHVQVRQQATLTPQQ Sbjct: 1 MFKKIWFQLHWFIGITAGTVLMTIGVTGALLAFREEIMDLLSPGVMHVQVRQQATLTPQQ 60 Query: 61 LLDKLREAEPARRVTTLTLTSEPGASARVVFAPPPGVRRGEVRYADPYTGALLPPVTGAE 120 +L+KLR+ EP RR+TT+TL++EPG S RVVFAPPPG RRG+ RY DPYTGALLPPVTG E Sbjct: 61 VLEKLRQEEPQRRITTMTLSAEPGDSVRVVFAPPPGERRGQARYVDPYTGALLPPVTGME 120 Query: 121 FFEFIEHFHRWLLLPTEIGKIVLGTLALALLMLALSGLYLRWPRRPLSLRAWLRLDFQLQ 180 FFEF E FHRWLLLPTEIGKIVLGTLAL LLMLALSGLYLRWPRR LSLRAWLRLDFQLQ Sbjct: 121 FFEFTERFHRWLLLPTEIGKIVLGTLALCLLMLALSGLYLRWPRRALSLRAWLRLDFQLQ 180 Query: 181 GRSFLWNLHSVIGTWSLVMYVVFSITGAYWALDWFKDGLNTLAGESRRPAMQRGEGKKK- 239 GRS LWNLHSVIGTWSLVMYV+F+ TGAYWALDWFKDG+NTLAGE+RR M RGEGKKK Sbjct: 181 GRSLLWNLHSVIGTWSLVMYVIFATTGAYWALDWFKDGVNTLAGETRRAPMARGEGKKKD 240 Query: 240 -PATDEAIPLELTQAWNSFLAKAPDYQSAQLRLPEQGSQPVQIFFLDHGAPHERARSRMV 298 P + L+LTQAWN+FL KAPDYQSAQLRLPEQG+QP+QIFFL H APHERARSRMV Sbjct: 241 GPNNQTPVALDLTQAWNAFLTKAPDYQSAQLRLPEQGTQPLQIFFLGHDAPHERARSRMV 300 Query: 299 VLPQSGEIRQLERYEDKSNGGKLIAAIYPLHMGTYFGLPGRIIMAIASLMMPLFGITGWM 358 VLPQSGEI+QLERYEDKS GG+ IAAIYPLHMGTYFGLPGRIIMAIASLMMPLFGITGWM Sbjct: 301 VLPQSGEIKQLERYEDKSTGGRFIAAIYPLHMGTYFGLPGRIIMAIASLMMPLFGITGWM 360 Query: 359 LYLDRRRKKAALRAERASLNGTAPAPEGEPILVAYASQTGQAERIALHTAAGLR------ 412 LYLDRRRKKAAL + G G +LVAYASQTGQAERIALHTAA LR Sbjct: 361 LYLDRRRKKAAL-RAERAALGEPQQQAGGQLLVAYASQTGQAERIALHTAARLRRSGVAV 419 Query: 413 -------------XHHRRILFIASTFGEGDAPDSARGFEKRLRQAAGQSLAHVHYAILAL 459 HH R+L IASTFGEGDAPDSARGFE+RLRQAAGQSLAH+HYA+LAL Sbjct: 420 TLQALAQTTAETLRHHPRLLLIASTFGEGDAPDSARGFERRLRQAAGQSLAHLHYAVLAL 479 Query: 460 GDRHYAQFCGFGHTLDHGLRQCGAKPLFPLVEVDRMAGVALRKWDTELAAHGLQASVSEL 519 GDRHY FCGFGHTLDHGLRQCGAKP+FPLVEVDRM ALRKW++ELA+HGL A+VS+L Sbjct: 480 GDRHYTSFCGFGHTLDHGLRQCGAKPMFPLVEVDRMDAAALRKWESELASHGLDAAVSDL 539 Query: 520 LPTTAPAWQQWKLTRRVLLNPGSQGAPLYHIELQPQDGS--LPDWQAGALVEILPRHAPD 577 LPT++P W W L R LN GSQG PL+H+ LQ D S P WQAGAL+E++PRHAPD Sbjct: 540 LPTSSPTWPSWVLVERHHLNAGSQGEPLFHLVLQSADSSQPAPAWQAGALLEVMPRHAPD 599 Query: 578 RIALLLQRLQLDGSAMVSHQGRQKTLADALACSVLPTHPVRDAQALADQLQPLAARSYSV 637 R+AL+LQRL LDG+ V +G + +LADALACSVLP DAQ LA QLQPL ARSYSV Sbjct: 600 RVALVLQRLGLDGATEVHCRGMRCSLADALACSVLPMQAAADAQTLAQQLQPLTARSYSV 659 Query: 638 ASVPQDGAVHLLVRQVRHEDGLGLASGWLTEFAPDDASIDLRLVENAGFAPVAADTPAIF 697 AS QDG V LLVRQ RHEDGLGLASGWLTE+AP A + RLV+NAGFAPV+ADTPAIF Sbjct: 660 ASAMQDGTVQLLVRQQRHEDGLGLASGWLTEYAPLGARLQARLVDNAGFAPVSADTPAIF 719 Query: 698 IGNGSGLAGLRGHLHARMQHGEANSRNWLLFGERNERYDFLYADELQDWLEHGRLARLDL 757 IGNGSG+AGLRGHL R+ G +RNWLLFGERNERYDFLY +ELQ WL+ G+LARLDL Sbjct: 720 IGNGSGMAGLRGHLRQRIHAG--GTRNWLLFGERNERYDFLYREELQAWLQQGQLARLDL 777 Query: 758 AFSRDQSERLYVQDRLREAGPELRQWLDQGAVIYVCGSLEGMAAGVDAVLTDLLGEAGLH 817 AFSRDQ R+YVQDRLREA ELR WLDQGAV+YVCGSL+GMAAGVDAVLT LLGE GL Sbjct: 778 AFSRDQEARIYVQDRLREAAGELRHWLDQGAVLYVCGSLDGMAAGVDAVLTSLLGETGLQ 837 Query: 818 ALIEQNRYRRDVY 830 LIEQNRYRRDVY Sbjct: 838 DLIEQNRYRRDVY 850 >gi|300311804|ref|YP_003775896.1| iron-regulated membrane protein [Herbaspirillum seropedicae SmR1] Length = 416 Score = 72.4 bits (176), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 96/416 (23%), Positives = 175/416 (42%), Gaps = 51/416 (12%) Query: 9 LHWFIGITAGTLLMTIGVTGAVLAFREEIMEALSPGVMHVQVRQQ----ATLTPQQLLDK 64 LH + G+ A L G+TGAV+++ E+ L+P + + Q A + +L + Sbjct: 8 LHRWFGLAAAVFLFIAGLTGAVISWDHELDAWLNPQLFDARSATQPGAGAPMPGLELARR 67 Query: 65 LREAEPARRVTTLTLTSEPGASARVVFAPPPGVRRGEVRY-------ADPYT-------- 109 + +AEP RVT + +EPG + ++ P G++ DP+T Sbjct: 68 VEQAEPRLRVTYVLTQAEPGHTIGMMVEPRVDPATGKLYTLDYNQIAVDPFTAEIQGRRM 127 Query: 110 -GALLPPVTGAEFFEFIEHFHRWLLLPT----EIGKIVLGTLALALLMLALSGLYLRWPR 164 GA+ ++ F+ H + LP E G +++G + + + L LYL +P Sbjct: 128 WGAI--SLSRENLLPFLYKLHYTMHLPDLGGLETGILLMGIIGIVWALDCLIALYLSFP- 184 Query: 165 RPLSLRAW---LRLDFQLQGRSFLWNLHSVIGTWSLVMYVVFSITGAYWALD--WFKDGL 219 + R+W L ++ G ++LH G W + ++ ++T L+ + L Sbjct: 185 ---NWRSWRKSLAFRWRDGGHKLNFDLHRSGGVWVWGLLLILAVTSVSMNLERQVMRPLL 241 Query: 220 NTLAG------ESRRPAMQRGEGKKKPATDEAIPLELTQAWNSFLAKAPD--YQSAQLRL 271 TL+ ++R P + + ++A+ L QA L + + S + Sbjct: 242 QTLSTLTPSPFDTRTPTAPEKVAEPRITREQAVALGQAQARKMGLTEPVGGVFYSGMFGV 301 Query: 272 PEQGS-QPVQIFFLDHGAPHERARSRMVVLPQSGEIRQLERYEDKSNGGKLIAAIYPLHM 330 G QP DHG + + SG + S G + A +PLH Sbjct: 302 YGVGYFQPGN----DHG-DIGLGNAWLYFDANSGAYEGGKIPGRGSAGDIFLQAQFPLHS 356 Query: 331 GTYFGLPGRIIMAIASLMMPLFGITGWMLYLDRRRKKAALRAERASLNGTAPAPEG 386 G GLPGRI+++ ++ + +TG ++++ R++KA L+ + PA EG Sbjct: 357 GRILGLPGRILISFMGALVAMLSVTGVIIWV--RKRKARLQQRERTAAQKTPAREG 410 >gi|300311746|ref|YP_003775838.1| iron-regulated membrane protein [Herbaspirillum seropedicae SmR1] Length = 435 Score = 64.7 bits (156), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 100/418 (23%), Positives = 167/418 (39%), Gaps = 59/418 (14%) Query: 9 LHWFIGITAGTLLMTIGVTGAVLAFREEIMEALSPGVMHVQV----RQQATLTPQQLLDK 64 LH + G+ L+ +TG++L F +E+ L+P + Q QA + P L +K Sbjct: 25 LHRWTGLGMAFFLIVTSLTGSLLVFDDELDAWLAPQLQLAQPAPGQSAQAMMDPYLLREK 84 Query: 65 LREAE-PARRVTTLTLTSEPGASARVVFAPPPGVRRGEVRYADPYTGALLPPVTG----- 118 + +A P ++ L L + PG + P G+ Y Y L+ P TG Sbjct: 85 VAQAMGPQVHISQLVLHARPGRTVSFQADPATDPATGK-PYRLGYDEILVNPYTGVVQGV 143 Query: 119 ----------AEFFEFIEHFHRWLLLPTEIGKIVLGTLALALLMLALSGLYLRWPR-RPL 167 A+ F+ H L LP G ++LGT+ L + G+ L WPR RP Sbjct: 144 RDRDKISLKPADLMPFLFKLHHSLALPRAPGALLLGTVGLLWTLDCFVGMILTWPRARPF 203 Query: 168 SLR---AWLRLDFQLQGRSFLWNLHSVIGTWSLVMYVVFSITGAYWALDWFKDGLNTLAG 224 ++ AWL + ++ +LH G W VM ++F+ W LN L Sbjct: 204 LVKWKPAWL-IKWRAGFYRVNLDLHRAFGLWCWVMLLLFA---------WSSVMLN-LRD 252 Query: 225 ESRRPAMQR--------GEGKKKPATDEAIPLELTQAWNSFLAKAPDYQSAQLRLPEQGS 276 + P M R G P P +A ++ + D + Q L Sbjct: 253 QIYLPVMSRILSFDTSWRPGPPLPQALSGPPQSWPEA-HALARRTMDGFARQRGLVIDFE 311 Query: 277 QPVQI-------FFLDHGAPHERA---RSRMVVLPQSGEIRQLERYEDKSNGGKLIAAIY 326 + + +L H RA + + + ++G+I +G + Sbjct: 312 EKLDFDRRRGVYIYLVHSNADLRADVGNTGLRIDARTGKILGHWLPVGDRDGNTFSNWLG 371 Query: 327 PLHMGTYFGLPGRIIMAIASLMMPLFGITGWMLYLDRRRKKAALRAE-RASLNGTAPA 383 LHMG FGLP RI++ + + L +TG +++ +K+AA R + R+ +G P+ Sbjct: 372 ALHMGHVFGLPWRILLVLVGGAVSLLTVTGVVVWW---KKRAARRLQLRSPGDGRKPS 426 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,829,447 Number of extensions: 127987 Number of successful extensions: 462 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 455 Number of HSP's successfully gapped: 3 Length of query: 830 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 737 Effective length of database: 1,179,515 Effective search space: 869302555 Effective search space used: 869302555 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 135 (56.6 bits)