BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0114.p (783 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310774|ref|YP_003774866.1| anaerobic formate dehydrogenase... 1474 0.0 gi|300311015|ref|YP_003775107.1| anaerobic (selenocysteine-conta... 727 0.0 gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydroge... 120 4e-28 gi|300312208|ref|YP_003776300.1| assimilatory nitrate reductase ... 107 5e-24 gi|300313158|ref|YP_003777250.1| anaerobic dehydrogenase family ... 60 5e-10 >gi|300310774|ref|YP_003774866.1| anaerobic formate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 790 Score = 1474 bits (3815), Expect = 0.0, Method: Compositional matrix adjust. Identities = 706/783 (90%), Positives = 736/783 (93%), Gaps = 10/783 (1%) Query: 1 MKTKDIPEEATFDEYNNAAGLVRLXESSAGGWGSVKALGAILQQEHVIATGTRILMKQNK 60 MKTKDIPE+ATFDEYNNAAG GWGSVKALG+ILQQEHVIA+GTRILM QNK Sbjct: 18 MKTKDIPEDATFDEYNNAAG----------GWGSVKALGSILQQEHVIASGTRILMHQNK 67 Query: 61 PDGFACVSCSWAKPADPHLFEFCENGAKATAWEITGKRATPEFFAQHTVTEMESWNDLEL 120 P+GFACVSCSWAKPADPHLFEFCENGAKATAWEIT KRAT FFAQHTVTEME+WNDLEL Sbjct: 68 PEGFACVSCSWAKPADPHLFEFCENGAKATAWEITRKRATAAFFAQHTVTEMEAWNDLEL 127 Query: 121 ESTGRITEPMKYDAASDRYLPVSWNEAFDDIAAQLRAMDPKKVVFYASGRASLETSYMYQ 180 ES+GR+TEPMKYDAASDRYLPVSW+EAFDDIAAQLRAMDPKKVVFYASGRASLETSY+YQ Sbjct: 128 ESSGRVTEPMKYDAASDRYLPVSWSEAFDDIAAQLRAMDPKKVVFYASGRASLETSYLYQ 187 Query: 181 LIARMYGTNNLPDSSNMCHESTSVALPQTIGVPVGTVRLDDFEHTGCILFFGHNTGTNAP 240 L+ARMYGTNNLPDSSNMCHESTSVALPQTIGVPVGTV LDDF T CILFFGHNTGTNAP Sbjct: 188 LMARMYGTNNLPDSSNMCHESTSVALPQTIGVPVGTVTLDDFGQTDCILFFGHNTGTNAP 247 Query: 241 RMLHQLEEARKRGVPVITFNPLRERGLERFVNPQSPKEMITPAHTDISSQYLQIRIGGDT 300 RMLH LEE RKRGVPVITFNPLRERGLERFVNPQSPKEM+TPAHTDIS+QYLQI+IGGD+ Sbjct: 248 RMLHPLEEVRKRGVPVITFNPLRERGLERFVNPQSPKEMVTPAHTDISTQYLQIKIGGDS 307 Query: 301 AVAIGMAKRILERDERARTQGLPRLLDQAFIDEHTSGFEEFAAAARAAEWAEIERHAGVS 360 A AIGMAKRILERD++AR +GLPRLLD AFI+EHTSGFE+FA A RAA W E+ERHAGVS Sbjct: 308 AAAIGMAKRILERDDKARQEGLPRLLDLAFIEEHTSGFEQFADAVRAAPWDELERHAGVS 367 Query: 361 RAELELAADTYAAADSAMLMYGMGITQHREAVRAIHMLTNLLLMRGNIGKPGAGICPIRG 420 RAELELAADTYA A AMLMYGMG+TQHREAVR IHMLTNLLLMRGNIGKPGAGICPIRG Sbjct: 368 RAELELAADTYAGAGRAMLMYGMGVTQHREAVRTIHMLTNLLLMRGNIGKPGAGICPIRG 427 Query: 421 HSNVQGQRTVGITEKPEMVPNDKLRALYHFEPPMEKGLNTVEACEKIRDGELSAFFMLGG 480 HSNVQGQRTVGITEKPE+VPNDKLRALYHFEPPMEKGLNTVEACEKIRDGELSAFFMLGG Sbjct: 428 HSNVQGQRTVGITEKPELVPNDKLRALYHFEPPMEKGLNTVEACEKIRDGELSAFFMLGG 487 Query: 481 NFVRAIPDHGVMEPAWRKLPLTVQVVTHFNRSCVIHGKTSYVLPCLGRIEIDQQRGGEQA 540 NFVRAIPDHGVME AWRKLPLTVQVVTHFNRSCVIHG+TSYVLPCLGRIEID+QRGGEQA Sbjct: 488 NFVRAIPDHGVMEAAWRKLPLTVQVVTHFNRSCVIHGQTSYVLPCLGRIEIDRQRGGEQA 547 Query: 541 VSVEDSTGCMHGSRGRVKPASPHLLSEPAILAELAKRLLPVNPQVDWDAWVGDYALIRDA 600 VSVEDSTGCMHGSRGR +PASPHLLSEPAI+AELAKRLLP NPQVDWD WVGDYA IRDA Sbjct: 548 VSVEDSTGCMHGSRGRAEPASPHLLSEPAIVAELAKRLLPFNPQVDWDGWVGDYARIRDA 607 Query: 601 IEATYPDIFRDFNARMWTPGGFARPLAARERQWKTRNGKANFKTPDGFDPDPDMPGDAPG 660 IEATYPDIFRDFNARMW PGGFARPLAARERQWKT NG+ANF TPDGFDPDPDMPGDAP Sbjct: 608 IEATYPDIFRDFNARMWNPGGFARPLAARERQWKTPNGRANFMTPDGFDPDPDMPGDAPE 667 Query: 661 VLRLMTTRGDSQFNTTVYSLDDRFRGVWGTRRVLLMNLRDLQQQGLGAGDFVTAETVSDD 720 VLRLMTTRGDSQFNTTVYSLDDRFRGVWGTRRVLLMNLRDLQQ GL AGDFV AETVS D Sbjct: 668 VLRLMTTRGDSQFNTTVYSLDDRFRGVWGTRRVLLMNLRDLQQHGLAAGDFVCAETVSAD 727 Query: 721 GVQRRVDHLRVEPFDIPVGCVMGYFPELNRLIPLFHHARGSKVPAAKSIPIRLIPEIGEP 780 GV+RRV HLRVEPFDIPVGCVMGYFPELNRLIPLFHHARGSKVPAAKS+PIRLIPE+ EP Sbjct: 728 GVERRVPHLRVEPFDIPVGCVMGYFPELNRLIPLFHHARGSKVPAAKSVPIRLIPELREP 787 Query: 781 VLS 783 LS Sbjct: 788 SLS 790 >gi|300311015|ref|YP_003775107.1| anaerobic (selenocysteine-containing) formate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 775 Score = 727 bits (1877), Expect = 0.0, Method: Compositional matrix adjust. Identities = 370/783 (47%), Positives = 491/783 (62%), Gaps = 33/783 (4%) Query: 6 IPEEATFDEYNNAAGLVRLXESSAGGWGSVKALGAILQQEHVI-ATGTRILMKQNKPDGF 64 +P + ++YN AGGWG++K + L +EHV G R L+ QN+PDGF Sbjct: 1 MPSKPRIEQYNQ----------PAGGWGALKYVALNLVKEHVADGRGLRTLLSQNQPDGF 50 Query: 65 ACVSCSWAKPADPHLFEFCENGAKATAWEITGKRATPEFFAQHTVTEMESWNDLELESTG 124 C C+W FEFCENG KA A E T KR TPEFFAQHTVTE+ +D ELE G Sbjct: 51 DCPGCAWPDREHTSTFEFCENGVKAVAAEATKKRVTPEFFAQHTVTELMQQSDFELEEHG 110 Query: 125 RITEPMKYDAASDRYLPVSWNEAFDDIAAQLRAM-DPKKVVFYASGRASLETSYMYQLIA 183 R+T PM YDAA+D+Y + W +AF +A L A+ DP FY SGRAS E ++++QL Sbjct: 111 RLTHPMVYDAATDKYKKIEWADAFALMAKHLNALPDPDMADFYVSGRASNEAAFLFQLFV 170 Query: 184 RMYGTNNLPDSSNMCHESTSVALPQTIGVPVGTVRLDDFEHTGCILFFGHNTGTNAPRML 243 R YGTNN PD SNMCHE TSV LP T+G+ GTV L+DF+H +L FG N TN PRM+ Sbjct: 171 RQYGTNNFPDCSNMCHEPTSVGLPGTVGIGKGTVLLEDFDHCDTLLLFGQNPATNHPRMM 230 Query: 244 HQLEEARKRGVPVITFNPLRERGLERFVNPQSPKEMITPAHTDISSQYLQIRIGGDTAVA 303 +L A KRG ++ NPL+ERGLERF +PQS EM+T T ISS ++ +GGD A+ Sbjct: 231 GELRHASKRGATIVAINPLKERGLERFADPQSKVEMLTMGSTRISSMFIHPTLGGDLALI 290 Query: 304 IGMAKRILERDERARTQGLPRLLDQAFIDEHTSGFEEFAAAARAAEWAEIERHAGVSRAE 363 G+ KR +E D+ AR G R++D FI+EHT+GFEEFAA ARA W +I +GVSR + Sbjct: 291 KGVIKRTIELDDAARAAGAERVIDVKFIEEHTAGFEEFAAQARAESWDDIVAESGVSRED 350 Query: 364 LELAADTYAAADSAMLMYGMGITQHREAVRAIHMLTNLLLMRGNIGKPGAGICPIRGHSN 423 +E A Y + + +GMG+TQH+ AV I +L+N++++RGNIG+ GAG+CP+RGHSN Sbjct: 351 IEKLAQVYVKGKAVIATWGMGLTQHKHAVATIQLLSNMMMLRGNIGRQGAGLCPVRGHSN 410 Query: 424 VQGQRTVGITEKPEMVPNDKLRALYHFEPPMEKGLNTVEACEKIRDGELSAFFMLGGNFV 483 VQG RTVGI EKP D+L ++ F+ P G +TV + + +G F LGGNF Sbjct: 411 VQGDRTVGIDEKPTPAFLDRLEQVFGFKAPRHHGNDTVGSVMAMLEGRTKVFIGLGGNFA 470 Query: 484 RAIPDHGVMEPAWRKLPLTVQVVTHFNRSCVIHGKTSYVLPCLGRIEIDQQRGGEQAVSV 543 A PD A R LTV + T NRS ++HGK + +LP +GR EID Q+ G Q V+V Sbjct: 471 MATPDTPRTFEAMRSCNLTVHITTKLNRSHLVHGKDALILPTMGRTEIDMQKSGPQGVTV 530 Query: 544 EDSTGCMHGSRGRVKPASPHLLSEPAILAELAKRLLPV---NPQVDWDAWVGDYALIRDA 600 EDS +H S G KPAS HL+SE AI+A LA+ + P++DW + DY+ IRDA Sbjct: 531 EDSMSMVHVSYGINKPASEHLMSETAIVAHLAEATMKSRNNGPKIDWLWYAEDYSRIRDA 590 Query: 601 IEATYPDIFRDFNARMWTPGGFARPLAARERQWKTRNGKANFKTPDGFDPDPDMPGDAP- 659 IE Y D F+ +N R+ PGGF +A+R+R WKT +GKANF D+P D P Sbjct: 591 IEQVY-DAFKGYNERIANPGGFHLGVASRDRVWKTASGKANFIV-------HDIPKDTPI 642 Query: 660 ---------GVLRLMTTRGDSQFNTTVYSLDDRFRGVWGTRRVLLMNLRDLQQQGLGAGD 710 +L LMTTR Q+NTT+Y +DDR+RGV+G RRV+ +N DLQ GL G+ Sbjct: 643 HRARAIHGEKLLTLMTTRSHDQYNTTIYGMDDRYRGVFGQRRVVFINPADLQMLGLKDGE 702 Query: 711 FVTAETVSDDGVQRRVDHLRVEPFDIPVGCVMGYFPELNRLIPLFHHARGSKVPAAKSIP 770 +V +V DDGV+RR + + ++IP GC+ Y+PE N L+PL A ++ P +KSIP Sbjct: 703 WVDITSVWDDGVERRAEGFLLVEYEIPRGCIGSYYPETNPLVPLESFADKARTPTSKSIP 762 Query: 771 IRL 773 + L Sbjct: 763 VLL 765 >gi|300310808|ref|YP_003774900.1| NAD-dependent formate dehydrogenase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 957 Score = 120 bits (301), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 86/317 (27%), Positives = 140/317 (44%), Gaps = 35/317 (11%) Query: 125 RITEPMKYDAASDRYLPVSWNEAFDDIAAQLRAMDPK----KVVFYASGRASLETSYMYQ 180 RI PM + +D + VSW EA A++ R + K + S R + E +Y+ Q Sbjct: 298 RILNPMIRKSINDPWREVSWEEALSYAASEFRRIQAKHGKDSIGGITSSRCTNEETYLVQ 357 Query: 181 LIARM-YGTNNLPDSSNMCHESTSVALPQTIGVPVGTVRLDDFEHTGCILFFGHNTGTNA 239 + R +G NN+ + +CH T L QT+G GT + + IL G N Sbjct: 358 KLVRAAFGNNNVDTCARVCHSPTGYGLKQTLGESAGTQTFESVMKSDVILIMGANPAAGH 417 Query: 240 PRMLHQLEEARKRGVPVITFNPLRERGLERFVNPQSPKEMITPAHTDISSQYLQIRIGGD 299 P Q++ ++G +I +P EM+ H + +L++R G + Sbjct: 418 PVFASQIKRRVRQGAKLIVIDP-------------RTTEMVKSPHIQ-ADYHLKLRPGTN 463 Query: 300 TAVAIGMAKRILERDERARTQGLPRLLDQAFIDEHT-----SGFEEFAAAARAAEWAEIE 354 TA+ +A IL L D+A+I E ++EF + A + Sbjct: 464 TAIITALAHVILSEG----------LQDEAYIQERCDLQSYQDWKEFVLREDNSPEA-MA 512 Query: 355 RHAGVSRAELELAADTYAAADSAMLMYGMGITQHREAVRAIHMLTNLLLMRGNIGKPGAG 414 GV + AA YA +A + YG+G+T+H + + + NL + GN+G+ G G Sbjct: 513 AITGVPAEIIRGAARLYATGGNAAIYYGLGVTEHAQGSTTVMGIANLAMCTGNVGREGVG 572 Query: 415 ICPIRGHSNVQGQRTVG 431 + P+RG +NVQG +G Sbjct: 573 VNPLRGQNNVQGSCDMG 589 >gi|300312208|ref|YP_003776300.1| assimilatory nitrate reductase large subunit [Herbaspirillum seropedicae SmR1] Length = 922 Score = 107 bits (266), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 106/459 (23%), Positives = 188/459 (40%), Gaps = 55/459 (11%) Query: 94 ITGKRATPEFFAQHTVTEMESWNDLELESTGRITEPMKYDAASDRYLPVSWNEAFDDIAA 153 I G P F + V ++L+ GR+ P DA + ++W++A D +A+ Sbjct: 40 IQGDAQHPANFGRLCVKGSALGETVDLD--GRLLHPQLRDAQG-QLQQLAWDQALDHVAS 96 Query: 154 QLRAM----DPKKVVFYASGRASLETSYMYQLIARMY-GTNNLPDSSNMCHESTSVALPQ 208 + A+ P V Y SG+ E Y+ + + Y G+ N+ +S +C S + Sbjct: 97 KFSAIIAEHGPDAVALYVSGQLLTEDYYIANKLMKGYIGSANIDTNSRLCMSSAVAGHKR 156 Query: 209 TIGVPVGTVRLDDFEHTGCILFFGHNTGTNAPRMLHQLEEARKR--GVPVITFNPLRERG 266 G + V +D E ++ G N P + ++ ++ + ++ +P R Sbjct: 157 AFGEDLVPVCYEDLELADLVVLVGSNAAWCHPILFQRIARLKETRPALRLVVIDPRRT-- 214 Query: 267 LERFVNPQSPKEMITPAHTDISSQYLQIRIGGDTAVAIGMAKRILERDERARTQGLPRLL 326 A +++ +L I+ G D + G+ + R R Sbjct: 215 ----------------ATCELADLHLPIKPGTDVWLFNGLMSYLAHRGLR---------- 248 Query: 327 DQAFIDEHTSGFEEFAAAARA--AEWAEIERHAGVSRAELELAADTYAAADSAMLMYGMG 384 D AFI+ HT GF+E A A A A+ A + R + +L + +A + + + MG Sbjct: 249 DNAFIEAHTKGFDEALAVADADCADPATVARLCKIDLKDLLAFYEAFANTEKVVTGFSMG 308 Query: 385 ITQHREAVRAIHMLTNLLLMRGNIGKPGAGICPIRGHSNVQGQRTVGITEKPEMVPNDKL 444 + Q ++ + N L G IGKPG G I G N G R VG D Sbjct: 309 VNQSSAGTDKVNSIINCHLATGRIGKPGMGPFSITGQPNAMGGREVGGLANMLAAHMDLD 368 Query: 445 RALYH------FEPPM---EKGLNTVEACEKIRDGELSAFFMLGGNFVRAIPDHGVMEPA 495 + L+ ++ P + GL V+ I +G + A +++ N + ++PD +++ A Sbjct: 369 QPLHRQIVQDFWQSPRIADKPGLKAVDMFRAIEEGRIKAVWIMATNPMVSLPDADLVQRA 428 Query: 496 WRKLPLTVQ--VVTHFNRSCVIHGKTSYVLPCLGRIEID 532 +K L V V+T + + + H +LP L E D Sbjct: 429 LQKCELVVSSDVITQTDTNALAH----VLLPALAWGEKD 463 >gi|300313158|ref|YP_003777250.1| anaerobic dehydrogenase family protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 60.5 bits (145), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 70/304 (23%), Positives = 115/304 (37%), Gaps = 46/304 (15%) Query: 125 RITEPMKY--DAASDRYLPVSWNEAFDDIA---AQLRAMDPKKVV--FYASGRASLETSY 177 R+ PM+ ++ +SW+EA IA Q+ DP+ ++ YA ++ Sbjct: 61 RLLHPMRRVGKKGEGKFERISWDEAIATIAQRLGQIALRDPQAILPYSYAGTMGQVQGES 120 Query: 178 MYQLIARMYGTNNLPDSSNMCHESTSVALPQTIGVPVGTVRLDDFEHTGCILFFGHNTGT 237 M G + L +C + TIG VG L+ +H IL +G N Sbjct: 121 MAGRFFHRLGASQL--ERTICASAGGAGYRYTIGARVGA-DLEQVDHAKLILIWGGNPIA 177 Query: 238 NAPRMLHQLEEARKRGVPVITFNPLRERGLERFVNPQSPKEMITPAHTDISSQYLQIRIG 297 + +++ A++ G +I +P R E+ Q++ + G Sbjct: 178 SNLHFWTRVQTAKRNGARLIAIDPYRSLTAEK------------------CHQHIALLPG 219 Query: 298 GDTAVAIGMAKRILERDERARTQGLPRLLDQAFIDEHTSGFEEFAAAARAAEW--AEIER 355 D A+A+GM ++ D LLD +I HT GFE+ R AEW A Sbjct: 220 TDAALALGMMHVLISED----------LLDHDYIARHTLGFEQL--RERVAEWTPARTAE 267 Query: 356 HAGVSRAELELAADTYA----AADSAMLMYGMGITQHREAVRAIHMLTNLLLMRGNIGKP 411 G+S E+ A Y A + + G+ + R A + L + G P Sbjct: 268 VCGISAEEVLNLAREYGRVALAGEGVFIRANYGLQRVRGGGMAARNVACLPALVGAWRHP 327 Query: 412 GAGI 415 G+ Sbjct: 328 AGGV 331 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,451,455 Number of extensions: 104221 Number of successful extensions: 276 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 266 Number of HSP's successfully gapped: 5 Length of query: 783 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 690 Effective length of database: 1,179,515 Effective search space: 813865350 Effective search space used: 813865350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)