BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0107.p (142 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310780|ref|YP_003774872.1| hypothetical protein Hsero_1448... 183 5e-48 >gi|300310780|ref|YP_003774872.1| hypothetical protein Hsero_1448 [Herbaspirillum seropedicae SmR1] Length = 146 Score = 183 bits (464), Expect = 5e-48, Method: Compositional matrix adjust. Identities = 104/146 (71%), Positives = 116/146 (79%), Gaps = 6/146 (4%) Query: 1 MNHDDNSIPLLTEIIPLAPAAPPAP-----DYAAPMQPGAFHTQPANTHYQAPAAAAPAM 55 MN DDNSIPLLTE+IPLAPAAP DYA P +PGAFHTQPA T YQA AP + Sbjct: 1 MNQDDNSIPLLTEVIPLAPAAPAHTPARAPDYAPPARPGAFHTQPAATSYQATPPGAPIV 60 Query: 56 TPLPPVTPAAEQYRQWEQEIRENVLQSLLERVDTVLHQHLQDQMALALDHLSDIVMQRVR 115 PLP VTPAAEQYRQWEQEIRENVLQSLLE+VDTVL Q LQDQMAL LD+LS ++ QR++ Sbjct: 61 APLP-VTPAAEQYRQWEQEIRENVLQSLLEQVDTVLQQQLQDQMALVLDNLSTVLTQRIK 119 Query: 116 EGLRDALTETVERAVAEEMARFTSSK 141 + L +AL ETV RAVAEEMARF S+K Sbjct: 120 DSLHEALAETVTRAVAEEMARFASTK 145 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.128 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 270,393 Number of extensions: 6759 Number of successful extensions: 48 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 46 Number of HSP's successfully gapped: 1 Length of query: 142 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 66 Effective length of database: 1,260,010 Effective search space: 83160660 Effective search space used: 83160660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)