BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0034.0102.p
         (307 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310784|ref|YP_003774876.1| gamma-glutamate-cysteine ligase...   569   e-164

>gi|300310784|ref|YP_003774876.1| gamma-glutamate-cysteine ligase
           [Herbaspirillum seropedicae SmR1]
          Length = 531

 Score =  569 bits (1467), Expect = e-164,   Method: Compositional matrix adjust.
 Identities = 277/307 (90%), Positives = 289/307 (94%)

Query: 1   LSEDTLFLPYGTSLRMSDLGYQNHAQANITPHYNNLPAYIRSLAHAVSEPYPPYEAIGTK 60
           LSEDTL+LPYGTSLRMSDLGYQNHAQANITPHYNNLPAYIRSL+HAVSEPYPPYEAIGTK
Sbjct: 225 LSEDTLYLPYGTSLRMSDLGYQNHAQANITPHYNNLPAYIRSLSHAVSEPYPPYEAIGTK 284

Query: 61  RDGEWVQINTNILQIENEFYSSIRPKRVINSGERPVQALSARGVQYVEVRCMDIDPFNPL 120
           RDG+WVQINTNILQIENEFYSSIRPKRVINSGERPVQALSARGVQYVEVRCMDIDPFNPL
Sbjct: 285 RDGQWVQINTNILQIENEFYSSIRPKRVINSGERPVQALSARGVQYVEVRCMDIDPFNPL 344

Query: 121 GIGLEAARFLDVFLHFLALESSCLTSDEEGVENRANFLATVKEGRRPGLKLQFKGESLLL 180
           GIGLEAARFLDVFLHFLALESSCLTSDEEG ENRANFLATVKEGRRPGL+L FKG S+ L
Sbjct: 345 GIGLEAARFLDVFLHFLALESSCLTSDEEGAENRANFLATVKEGRRPGLQLHFKGASIGL 404

Query: 181 TDWGQQLLDRMEPVADLLDRHLGGTQHRDSLAVQRAKLADPDSTPSARVLREIREAGNSF 240
             WG+QLLDRM PVADLLDR  GG+QHRDSL  QRAKLADPDSTPSARVLREIR +GNSF
Sbjct: 405 KAWGEQLLDRMAPVADLLDRQRGGSQHRDSLQAQRAKLADPDSTPSARVLREIRASGNSF 464

Query: 241 PAYTLANSQRQAAELIARPLSSSKTADFVAIAQASLDAQRDIERTQVGDFDTFVAAYRAS 300
             YTLA+SQRQAAEL+ARPL ++ TADFVAIAQASLDAQR IERTQ GDFDTFVAAYRAS
Sbjct: 465 ADYTLASSQRQAAELMARPLPAATTADFVAIAQASLDAQRAIERTQEGDFDTFVAAYRAS 524

Query: 301 TLGNIAI 307
           TLGNIAI
Sbjct: 525 TLGNIAI 531


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.318    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 921,327
Number of extensions: 36437
Number of successful extensions: 98
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 98
Number of HSP's successfully gapped: 1
Length of query: 307
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 222
Effective length of database: 1,217,395
Effective search space: 270261690
Effective search space used: 270261690
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 130 (54.7 bits)