BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0081.p (389 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311780|ref|YP_003775872.1| major facilitator superfamily p... 712 0.0 gi|300313056|ref|YP_003777148.1| major facilitator superfamily p... 121 9e-29 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 120 1e-28 gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 107 2e-24 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 105 9e-24 >gi|300311780|ref|YP_003775872.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 712 bits (1837), Expect = 0.0, Method: Compositional matrix adjust. Identities = 370/389 (95%), Positives = 383/389 (98%) Query: 1 VLVFPAVFFPNEPKLEGTLYAFVIFAFAFLARPIGTTIFMAIQKHYGREVKLTLALFMLG 60 VLVFPAVFFPNEPKLEGTLYAFVIFAFAFLARPIGTTIFMAIQKHYGREVKLTLALFMLG Sbjct: 59 VLVFPAVFFPNEPKLEGTLYAFVIFAFAFLARPIGTTIFMAIQKHYGREVKLTLALFMLG 118 Query: 61 FSTAGIALLPTYEHIGAWAIILLSLLRVGQGVALGGAWDGLPSLLAMNAPPNKRGWYAAL 120 FSTAGIALLPT+EHIG WAIILLSLLRVGQG+ALGGAWDGLPSLLAMNAP +KRGWYAAL Sbjct: 119 FSTAGIALLPTFEHIGVWAIILLSLLRVGQGLALGGAWDGLPSLLAMNAPQHKRGWYAAL 178 Query: 121 GQLGAPLGFVIAASLFAYMLATLTTTDFLEWGWRFPFYVAFAINVVALFSRLRLVSAPEY 180 GQLGAPLGFV+AASLFAYML++LTT DFLEWGWR+PFYVAFAINVVALFSRLRLVSAPEY Sbjct: 179 GQLGAPLGFVVAASLFAYMLSSLTTADFLEWGWRYPFYVAFAINVVALFSRLRLVSAPEY 238 Query: 181 AKLLDERELEPAGVVELATTQGRNIIIGALAALASYALFHLVTVFPLSWIKLYSLREASA 240 AKLLDERELEPAGVVELATTQGRNIIIGALAALASYALFHLVTVFPLSWIKLYSLREA+ Sbjct: 239 AKLLDERELEPAGVVELATTQGRNIIIGALAALASYALFHLVTVFPLSWIKLYSLREATD 298 Query: 241 FLVIQIWGGVIMAAGIVVSGLIADRFGRRNTLGSMALLIAILSAFVPSLMNGGESGQNTF 300 FLV+QIWGG+IMA GIV+SGLIADRFGRRNTLGSMALLIAILSAFVP+LMNGGE+GQNTF Sbjct: 299 FLVVQIWGGLIMAVGIVISGLIADRFGRRNTLGSMALLIAILSAFVPTLMNGGEAGQNTF 358 Query: 301 ILIGFALLGLSYGQAAGAVTANFKARYRYTGAALTSDLAWLVGAGFAPLVALGLSAHFGM 360 ILIGFALLGLSYGQAAGAVTANFKARYRYTGAALTSDLAWLVGAGFAPLVALGLSAHFGM Sbjct: 359 ILIGFALLGLSYGQAAGAVTANFKARYRYTGAALTSDLAWLVGAGFAPLVALGLSAHFGM 418 Query: 361 AYVSFYLLSGAVGSLAALSLNRALEGRDD 389 AYVSFYLLSGAVGSLAALSLNRALE RDD Sbjct: 419 AYVSFYLLSGAVGSLAALSLNRALEARDD 447 >gi|300313056|ref|YP_003777148.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 465 Score = 121 bits (304), Expect = 9e-29, Method: Compositional matrix adjust. Identities = 77/276 (27%), Positives = 139/276 (50%), Gaps = 14/276 (5%) Query: 2 LVFPAVFFPNEPKLEGTLYAFVIFAFAFLARPIGTTIFMAIQKHYGREVKLTLALFMLGF 61 L+FP +FFP + + + ++ARPIG + + +GR+ L +F++GF Sbjct: 40 LIFPQLFFPKGDPTTAIIASLATYGVGYVARPIGAFVLGHLGDTHGRKKVLLWCMFLMGF 99 Query: 62 STAGIALLPTYEHIGAWAIILLSLLRVGQGVALGGAWDGLPSLLAMNAPPNKRGWYAALG 121 ST G+ LLPTY+ +G WA LL ++R+ QG A+ G G S++ ++P +RG++++ Sbjct: 100 STIGVGLLPTYDQVGLWAPALLVIMRLAQGFAVAGEISGASSMILEHSPFGRRGYFSSFT 159 Query: 122 QLGAPLGFVIAASLFAYMLATLTTTDFLEWGWRFPFYVAFAINVVALFSRLRLVSAPEYA 181 G G ++AA++F + + F WGWR PF ++F + + R + P + Sbjct: 160 LQGVQAGQILAAAVFLPLAHYMPAEQFNVWGWRIPFLLSFLVIIAGWVIRRTVDETPVF- 218 Query: 182 KLLDERELE-PAGVVELATTQGRNIIIGALAALASYALFHLVTVFPLSWIKLYSLREA-- 238 K D+ EL+ + VV+ N++ + AL +++ V + Y+++ A Sbjct: 219 KAADKEELKRRSPVVDAFRDCSPNML-----RVVCMALMNVIPVVATIFGAAYAVQPAYG 273 Query: 239 -----SAFLVIQIWGGVIMAAGIVVSGLIADRFGRR 269 +L I + G ++ I G ++DR GRR Sbjct: 274 IGFAKDVYLWIPVVGNIVAVLVIPYVGKLSDRIGRR 309 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 120 bits (302), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 100/382 (26%), Positives = 176/382 (46%), Gaps = 10/382 (2%) Query: 7 VFFPNEPKLEGTLYAFVIFAFAFLARPIGTTIFMAIQKHYGREVKLTLALFMLGFSTAGI 66 +FFP + + L A F F RP+G + GR+ + + + ++ + A I Sbjct: 38 LFFPADNEYTSLLVALATFGIGFFMRPVGGVLLGLYADRKGRKAAMQVIIVLMTLAIAII 97 Query: 67 ALLPTYEHIGAWAIILLSLLRVGQGVALGGAWDGLPSLLAMNAPPNKRGWYAALGQLGAP 126 A PTY IG A I++ + R+ QG A GG + + L +AP +KRG Y + +G Sbjct: 98 AFTPTYATIGIAAPIMIVVARMLQGFATGGEYSSSTAFLVESAPAHKRGLYGSWQLVGQC 157 Query: 127 LGFVIAASLFAYMLATLTTTDFLEWGWRFPFYVAFAINVVALFSRLRLVSAPEYAKLLDE 186 L A + A + L+ WGWR PF + I V L+ R + ++ + ++ Sbjct: 158 LAVFCGAGMGALVTRNLSPEALDSWGWRLPFVLGLLIGPVGLWIRRHMHETDDFLESANK 217 Query: 187 RELEPAGVVELATTQGRNIIIGALAALASYALFHLVTV-FPLSWIKLYSLREASAFLVIQ 245 P ++++ T R +++ + F++V V P K L AF+V Q Sbjct: 218 PNAAPIKLLDVVKTNLRGVLVSMGQVINGTVAFYVVLVNMPTFANKQLGLPLDQAFMV-Q 276 Query: 246 IWGGVIMAAGIVVSGLIADRFGRRNTL--GSMALLIAILSAFVPSLMNGGESGQNTFIL- 302 + ++ A I +G ++DR GRR L S+A L+ + FV ++ S ++ Sbjct: 277 MIAVALLTAVIPFAGALSDRLGRRTVLLYSSLAFLLMVYPLFV--WVSAAPSLPRLLVMQ 334 Query: 303 -IGFALLGLSYGQAAGAVTANFKARYRYTGAALTSDLAWLVGAGFAPLVALGLSAHFG-- 359 + ++G ++G A++ F R R TG A+ +LA ++ GFAP + L+ G Sbjct: 335 VLLCIVIGANFGPMPTALSEQFPTRVRSTGLAVAYNLAAMLFGGFAPFIVTWLTKTSGSP 394 Query: 360 MAYVSFYLLSGAVGSLAALSLN 381 +A + L + +G AA ++ Sbjct: 395 VAPAWYVLFAACIGVSAAWAMR 416 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 107 bits (266), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 99/353 (28%), Positives = 168/353 (47%), Gaps = 12/353 (3%) Query: 8 FFPNEPKLEGTLYAFVIFAFAFLARPIGTTIFMAIQKHYGREVKLTLALFMLGFSTAGIA 67 FFP++ + + F+ F FL RP+G I A GR L + L ++ T IA Sbjct: 42 FFPSDNEFASLMLTFMTFGAGFLMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIA 101 Query: 68 LLPTYEHIGAWAIILLSLLRVGQGVALGGAWDGLPSLLAMNAPPNKRGWYAALGQLGAPL 127 +P+Y IGA A +L+ + R+ QG + G G+ LA A P +G+Y + + Sbjct: 102 FVPSYATIGALAPLLVLIGRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQV 161 Query: 128 GFVIAASLFAYMLAT-LTTTDFLEWGWRFPFYVAFAINVVALFSRLRLVSAPEYAKLLDE 186 ++AA++ Y+L+T LT + WGWR PF++ I V R L E+ + Sbjct: 162 AIIVAAAI-GYLLSTSLTPAEVSGWGWRIPFFIGCLIVPVLFVIRRSLQETEEFMR---- 216 Query: 187 RELEPA-GVVELATTQGRNIIIGALAALASYAL-FHLVTVFPLSWIKLYSLREASAFLVI 244 R+ P+ G + + + ++I + ++ + F+L+TV+ ++ K A L++ Sbjct: 217 RKHRPSTGQIFRSMIENWKLVIAGMMLVSMTTVSFYLITVYTPTFGKNVLKLSTEASLIV 276 Query: 245 QIWGGVIMAAGIVVSGLIADRFGRRNTLGSMALLIAILSAF--VPSLMNGGESGQNTFIL 302 + G+ + + G ++DR GRR L + +L IL+A+ V L++ + + Sbjct: 277 TLCVGLSNFIWLPIMGALSDRVGRRPLLVTFTVL-TILTAYPAVNWLVHDASFAKMLIVE 335 Query: 303 IGFALLGLSY-GQAAGAVTANFKARYRYTGAALTSDLAWLVGAGFAPLVALGL 354 + + L SY G A+T A R G +L LA V GF P +A GL Sbjct: 336 LWLSFLYASYNGAMVVALTEVMPADVRTAGFSLAYSLATAVFGGFTPAIATGL 388 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 105 bits (261), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 91/349 (26%), Positives = 157/349 (44%), Gaps = 7/349 (2%) Query: 2 LVFPAVFFPNEPKLEGTLYAFVIFAFAFLARPIGTTIFMAIQKHYGREVKLTLALFMLGF 61 ++ +FFP L L A F F RP+G + GR+ L++ + ++ Sbjct: 45 VIIAKLFFPTGNDLTSLLLAVATFGVGFFMRPVGGILLGIYADRVGRKAALSVTILLMAV 104 Query: 62 STAGIALLPTYEHIGAWAIILLSLLRVGQGVALGGAWDGLPSLLAMNAPPNKRGWYAALG 121 T I L PTYE IG +A +++ + R+ QG + GG + L AP +R +Y++ Sbjct: 105 GTTMIGLAPTYESIGLFAPLIIVVARLLQGFSAGGEMGSATAFLTEYAPERERAYYSSWI 164 Query: 122 QLGAPLGFVIAASLFAYMLATLTTTDFLEWGWRFPFYVAFAINVVALFSRLRLVSAPEYA 181 Q + ++ A++ ++ A+L WGWR PF + I V + R L P + Sbjct: 165 QASIGVAVLLGAAVGTFVTASLDAAALASWGWRLPFLLGIIIGPVGYYIRHHLEETPTF- 223 Query: 182 KLLDERELEPAGVVELATTQGRNIIIG-ALAALASYALFHLVTVFPLSWIKLYSLREASA 240 L+E++ + + E+ R ++ L + + L+ P +K+ L + + Sbjct: 224 --LEEKDKTDSPLKEVIRNFPRETSASFSMVILWTVCTYVLLFYMPTYSVKVLKLPQTTG 281 Query: 241 FLVIQIWGGVIMAAGIVVSGLIADRFGRRNTLGSMALLIAILSAFVPSLMNGGESGQN-- 298 F+ + G IM +V G +ADR GRR L A LI +L+ + + +N S Sbjct: 282 FIAGMVGGLCIMVLAPIV-GRLADRIGRRPLLSGSAALILMLAYPMFAYINSAPSLSTLL 340 Query: 299 TFILIGFALLGLSYGQAAGAVTANFKARYRYTGAALTSDLAWLVGAGFA 347 F L+ L+ G A + F A+ TG ++ +LA + GFA Sbjct: 341 IFQLVFGTLIAAYTGPILAAFSELFPAKVLSTGLSVAYNLAVTIFGGFA 389 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.141 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,247,508 Number of extensions: 55895 Number of successful extensions: 314 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 293 Number of HSP's successfully gapped: 11 Length of query: 389 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 302 Effective length of database: 1,207,925 Effective search space: 364793350 Effective search space used: 364793350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)