BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0076.p (146 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311776|ref|YP_003775868.1| ubiquinol oxidase subunit IV pr... 227 3e-61 >gi|300311776|ref|YP_003775868.1| ubiquinol oxidase subunit IV protein [Herbaspirillum seropedicae SmR1] Length = 158 Score = 227 bits (579), Expect = 3e-61, Method: Compositional matrix adjust. Identities = 111/126 (88%), Positives = 118/126 (93%) Query: 21 TMKDYVTGFVLAVILTAIPFWMVMNKTIANPATLGIVLLVFAAVQIVVHIKYFLHMNSKS 80 TMKDYVTGFVLAVILTAIPFW+VMNK+I++ AT+GIVLL AAVQIVVHIKYFLHMN KS Sbjct: 33 TMKDYVTGFVLAVILTAIPFWLVMNKSISSSATMGIVLLALAAVQIVVHIKYFLHMNGKS 92 Query: 81 EGGWNMLAMIFTVVIVVIALSGSLWVMYHLNHNMMPTMSPENMPTETVPQSMQGMQGMQH 140 EGGWNMLAMIFT+VIVVI L+GSLWVMYHLNHNMMPTMSPENMPTETVPQSMQGMQGMQH Sbjct: 93 EGGWNMLAMIFTIVIVVITLAGSLWVMYHLNHNMMPTMSPENMPTETVPQSMQGMQGMQH 152 Query: 141 GAAPSK 146 A SK Sbjct: 153 SAPQSK 158 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.135 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 339,412 Number of extensions: 10823 Number of successful extensions: 52 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 52 Number of HSP's successfully gapped: 1 Length of query: 146 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 69 Effective length of database: 1,255,275 Effective search space: 86613975 Effective search space used: 86613975 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 126 (53.1 bits)