BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0074.p (428 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311774|ref|YP_003775866.1| two component sensor histidine ... 794 0.0 gi|300311910|ref|YP_003776002.1| transmembrane sensor histidine ... 110 2e-25 gi|300313027|ref|YP_003777119.1| two component transmembrane sen... 61 2e-10 gi|300312655|ref|YP_003776747.1| C4-dicarboxylate transport sens... 60 4e-10 gi|300311800|ref|YP_003775892.1| two component heavy metal senso... 58 2e-09 >gi|300311774|ref|YP_003775866.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 460 Score = 794 bits (2051), Expect = 0.0, Method: Compositional matrix adjust. Identities = 394/428 (92%), Positives = 407/428 (95%) Query: 1 MLLLIQLRWLAVLGQVSTIAFVVLVFDLHLPLPHMLEVLSCLIAFNIASHLRWHERRIAT 60 MLLLIQLRWLAVLGQVSTIAFVVLVFDLHLPLPHMLEVLSCL+AFNIASHLRWHERRIAT Sbjct: 33 MLLLIQLRWLAVLGQVSTIAFVVLVFDLHLPLPHMLEVLSCLVAFNIASHLRWHERRIAT 92 Query: 61 NAELFLALLVDVAVLTFQLYLSGGITNPFAFLYLMQIILSAVLLKPWSSWAIVAVTMACL 120 NAELFLALLVDVAVLTFQLYLSGGITNPFAFLYLMQIILSAVLLKPWSSWAIV VTM CL Sbjct: 93 NAELFLALLVDVAVLTFQLYLSGGITNPFAFLYLMQIILSAVLLKPWSSWAIVGVTMLCL 152 Query: 121 AGLALFSEPLALPPDHNAGLLSLYVEGTLICFLLNAGLMVFFITRISSNLRAGDALLADL 180 AGLALFSEPLALPPDHNAGLLSLYVEGTLICFLLNAGLMVFFITRISSNLRAGDALLADL Sbjct: 153 AGLALFSEPLALPPDHNAGLLSLYVEGTLICFLLNAGLMVFFITRISSNLRAGDALLADL 212 Query: 181 RQRAAEEDHIVRMGLLASGAAHELGTPLATVSVILGDWKRMPEISQRADMLEDIAEMEAQ 240 RQRAAEEDHIVRMGLLASGAAHELGTPLATVSVILGDWKRMPEISQR DML+DIAEMEAQ Sbjct: 213 RQRAAEEDHIVRMGLLASGAAHELGTPLATVSVILGDWKRMPEISQRQDMLDDIAEMEAQ 272 Query: 241 LQRCKSIVSGVLLSAGEARGESSIKTTINTFLDSVAEDWRKTRPVEIFEYRNLIGQDVPV 300 LQRCKSIVSGVLLSAG+ARGESSIKTT+NTFLDSVAEDWRK+RPV +F+Y+NLIGQD+PV Sbjct: 273 LQRCKSIVSGVLLSAGQARGESSIKTTLNTFLDSVAEDWRKSRPVAVFDYQNLIGQDLPV 332 Query: 301 AFDSAIKQMLGNVLDNAFEVSPQWLALEVRREGDLLLMEVSDRGPGFPDWMINQIGKPYQ 360 AFDSAIKQMLGNVLDNAFEVSP WL+LEVRREG LLLMEVSD+GPGFP+WMINQIGKPYQ Sbjct: 333 AFDSAIKQMLGNVLDNAFEVSPHWLSLEVRREGGLLLMEVSDQGPGFPEWMINQIGKPYQ 392 Query: 361 STKGKPGRGLGLFFVVNVARKLGGRVWASNRAAGGARVTLELPLSAISLAPLTESAPGSE 420 STKGKPGRGLGLFFVVNVARKLGGRVWASN GARVTLELPLSAISL E GS Sbjct: 393 STKGKPGRGLGLFFVVNVARKLGGRVWASNLPGAGARVTLELPLSAISLEADAEPVVGSA 452 Query: 421 LPDYPMGA 428 P +P GA Sbjct: 453 TPAFPSGA 460 >gi|300311910|ref|YP_003776002.1| transmembrane sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 443 Score = 110 bits (276), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 130/422 (30%), Positives = 207/422 (49%), Gaps = 27/422 (6%) Query: 4 LIQLRWLAVLGQVSTIAFVVLVFDLHLPLPHMLEVLSCLIAFNIASHLRWHERRIATNAE 63 L LRW V G++ +A + LP+P +L ++ N A+ LR R A E Sbjct: 24 LFWLRWAMVAGELLVLAGAHGWLHIALPVPQLLALIGLQALLNGATWLRLRWTRPAEQRE 83 Query: 64 LFLALLVDVAVLTFQLYLSGGITNPFAFLYLMQIILSAVLLKPWSSWAIVAVTMACLAGL 123 LFL LL+D+AVLT LY SGG TNPF YL + A L + + + +GL Sbjct: 84 LFLQLLLDLAVLTALLYFSGGSTNPFVSFYLPALAAGAASLAWPYALLLALCALGAYSGL 143 Query: 124 ALFSEPLALPPDHNAG-LLSLYVEGTLICFLLNAGLMVFFITRISSNLRAGDALLADLRQ 182 EPL + H+ G ++ ++ G I F ++A L+ +F++RI++ +R+ + LA R+ Sbjct: 144 VYVYEPLHI---HDQGQAMAYHLAGMWINFSISALLITWFVSRIAAAVRSRETELARARE 200 Query: 183 RAAEEDHIVRMGLLASGAAHELGTPLATVSVILGDWKR-MPEISQRADMLEDIAEMEAQL 241 R + I+ +G A+GAAH L TPL+TV+VI G+ +R M A E++ +E Q+ Sbjct: 201 RQLQSQRILALGTQAAGAAHALNTPLSTVAVIAGELRRDMATQPALAAYDEELRVVEEQI 260 Query: 242 QRCKSIVSGVLLSA-GEARGESSIKTTINTFLDSVAEDWRKTRPVEIFEYRNLIGQDVPV 300 CK+ + + A + R E S I +L ++ E WR P + + Q V Sbjct: 261 AVCKAALESMRQDADAQTRNEDS--APITPWLTTLLEGWRLRHPAVLLSAE--VHQPGWV 316 Query: 301 AFD-SAIKQMLGNVLDNAFEV-----SPQWLALEVRREGD--------LLLMEVSDRGPG 346 A + Q+L +LDNA S +++ +R + ++E++D G G Sbjct: 317 AARLEDLSQILLTLLDNAAHAVRERGSEGRVSIRMRAADEPAGRPAQKAAIIEIADNGSG 376 Query: 347 FPDWMINQIGK-PYQSTKGKPGRGLGLFFVVNVARKLGGRVWASNRAAGGARVTLELPLS 405 ++ ++G+ P S+ G G GL L F A LGG++ + G L LPLS Sbjct: 377 IAPELLQRLGRAPVASSSGGSGIGLMLAFA--SAAHLGGKLRLYSTPGQGTCAELRLPLS 434 Query: 406 AI 407 A+ Sbjct: 435 AM 436 >gi|300313027|ref|YP_003777119.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 454 Score = 60.8 bits (146), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 76/297 (25%), Positives = 126/297 (42%), Gaps = 65/297 (21%) Query: 173 GDALLADLRQRAAEE------------------DHIVRMGLLASGAAHELGTPLATVSV- 213 G + D QR AEE + + +G LA+G AHE+ P+ VS Sbjct: 149 GRKEMEDTLQRQAEEQRKLIEQLKMAQAQLMQSEKLASIGQLAAGVAHEINNPIGFVSAN 208 Query: 214 --ILGDW-------------------------KRMPEISQRADML---EDIAEMEAQ--- 240 LG++ +++ RAD+ ED+ E+ A+ Sbjct: 209 LNTLGNYVATLLEGIQLHQCLQGGALPAAMAQEQLRSFEARADLAFLSEDVTELLAESNE 268 Query: 241 -LQRCKSIVSGVLLSAGEARGESSIK-TTINTFLDS---VAEDWRKTRPVEIFEYRNLIG 295 LQR ++IV L GES+ + T ++ LDS +A + K + + +Y L Sbjct: 269 GLQRVRNIVQS--LKDFSRIGESNWQMTDLHRGLDSTLNIANNELKYKVSIVKQYGEL-- 324 Query: 296 QDVPVAFDSAIKQMLGNVLDNAFEVSPQ--WLALEVRREGDLLLMEVSDRGPGFPDWMIN 353 + S + Q+ N+L NA + + + + R GD + + +SD G G +N Sbjct: 325 -PLVKCLASELNQVFMNLLVNAAQAISERGTVTICTARAGDKVSVSISDTGHGIAPENLN 383 Query: 354 QIGKPYQSTKG-KPGRGLGLFFVVNVARKLGGRVWASNRAAGGARVTLELPLSAISL 409 +I +P+ +TK G GLGL + +K GG + S+ G T+ELP+ S+ Sbjct: 384 RIFEPFFTTKPVGSGTGLGLSLSYGIVKKHGGDIRVSSAPGCGTTFTIELPIDPASV 440 >gi|300312655|ref|YP_003776747.1| C4-dicarboxylate transport sensor kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 600 Score = 60.1 bits (144), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 58/243 (23%), Positives = 105/243 (43%), Gaps = 9/243 (3%) Query: 169 NLRAGDALLADLRQRAAEEDHIVRMGLLASGAAHELGTPLATVSVILGDWKRM---PEIS 225 L+ ++LL + + + +G +A+G HEL PL + + + + + Sbjct: 355 KLQQTESLLRTTQNELVQAGKLAMLGQMAAGVTHELNQPLTAIRAFADNAVKFLARGQSA 414 Query: 226 QRADMLEDIAEMEAQLQRCKSIVSGVLLSAGEARGESSIKTTINT---FLDSVAEDWRKT 282 Q + LE I+ A + + + + G +GEA + I L S + T Sbjct: 415 QAVENLEYISSASATMGKIIAQLKGFARKSGEAVACVDLSQAIEASSLLLASECQKLGAT 474 Query: 283 RPVEIFEYRNLIGQDVPVAFDSAIKQMLGNVLDNAFEVSPQWLALEVRREGDLLLMEVSD 342 ++I + G DV V + + +L N LD E + +A+ + G L+ + D Sbjct: 475 ISIDIRAAACVTG-DV-VRTEQVLVNLLRNALDAVEEAEHKRIAVVLEVAGGYALVRIRD 532 Query: 343 RGPGFPDWMINQIGKPYQSTKGK-PGRGLGLFFVVNVARKLGGRVWASNRAAGGARVTLE 401 G G D + + +P+ +TK G GLGL ++ + + G++ A N GGA + Sbjct: 533 SGGGIADEVAPHLFEPFFTTKSSSKGLGLGLAISSSIVQAMNGQLSAHNHEEGGAEFVVR 592 Query: 402 LPL 404 LPL Sbjct: 593 LPL 595 >gi|300311800|ref|YP_003775892.1| two component heavy metal sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 57.8 bits (138), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 60/225 (26%), Positives = 106/225 (47%), Gaps = 29/225 (12%) Query: 187 EDHIVRMGLLASGAAHELGTPLATVSVILGDWKRMPEISQRADMLEDIAEME-AQLQRCK 245 ED ++ + AHEL TPLA ++G+ + + A +D+ + +L R + Sbjct: 239 EDAYTQLEAFNADVAHELRTPLAN---LIGETQVALARERSAGEFKDVLQSNLEELDRIR 295 Query: 246 SIVSGVLLSAGEARGESSIKTTINTFLDSVAEDWRKTRPVEIFEYR-NLIGQDVPVAFDS 304 SIV+ +L A +GE++ VA++ +KT V+ FE+ + G + VA D+ Sbjct: 296 SIVNDMLFLARSDQGEAATARVPA----RVADEVQKT--VDFFEFVLDDSGMRIEVAGDT 349 Query: 305 A---------IKQMLGNVLDNAFEVSPQ--WLALEVRREGDLLLMEVSDRGPGFPDWMI- 352 K+ L N++ NA + SP+ L +++ + + + + VS+ G + + Sbjct: 350 EATAMMETALFKRALTNLIQNAIQHSPEGALLRIDIACQEETVRVAVSNPGKPIDEHHLP 409 Query: 353 ------NQIGKPYQSTKGKPGRGLGLFFVVNVARKLGGRVWASNR 391 ++ Q G G GLGL V VAR GGR +AS++ Sbjct: 410 RLFDRFYRVDSARQDINGSHGHGLGLAIVKAVARMHGGRAFASSK 454 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.138 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,283,705 Number of extensions: 51001 Number of successful extensions: 273 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 280 Number of HSP's successfully gapped: 11 Length of query: 428 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 340 Effective length of database: 1,203,190 Effective search space: 409084600 Effective search space used: 409084600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)