BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0072.p (404 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311772|ref|YP_003775864.1| branched-chain amino acid ABC t... 753 0.0 gi|300310957|ref|YP_003775049.1| branched-chain amino acid ABC t... 352 4e-98 gi|300312236|ref|YP_003776328.1| amino acid ABC transporter peri... 88 1e-18 gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC t... 85 9e-18 gi|300313292|ref|YP_003777384.1| branched-chain amino acid ABC t... 78 1e-15 >gi|300311772|ref|YP_003775864.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 753 bits (1945), Expect = 0.0, Method: Compositional matrix adjust. Identities = 364/404 (90%), Positives = 390/404 (96%) Query: 1 VNHFNFQRGLLWACAATIVNLSAGAAQAQETIKIGEINSYKAQPAFLEPYRRGWEMALDE 60 +N FNFQRGL WA AA ++ + + AA+AQ+TI+IGEINSYKAQPAFLEPYRRGWEMALDE Sbjct: 1 MNQFNFQRGLRWALAAALLTMGSVAARAQDTIRIGEINSYKAQPAFLEPYRRGWEMALDE 60 Query: 61 VNAAGGVLGKKLEVIARDDNGNPGDSVRVAEELMARERVALLFGGFLSNTGLALTDYAKQ 120 +NA+GGVLGKKLEVIARDDNGNPGDSVRVAEELMARERVALLFGGFLSNTGLALTDYAKQ Sbjct: 61 INASGGVLGKKLEVIARDDNGNPGDSVRVAEELMARERVALLFGGFLSNTGLALTDYAKQ 120 Query: 121 HKVFFLAAEPLTDKIVWQNGNKYTYRLRPSTYMLVASVVKEAAKLKKKRWAIVYPNYEYG 180 H+VFFLAAEPLTDKIVWQNGNKYTYRLRPST MLVASVVKEA KLKKKRWAIVYPNYEYG Sbjct: 121 HRVFFLAAEPLTDKIVWQNGNKYTYRLRPSTSMLVASVVKEAVKLKKKRWAIVYPNYEYG 180 Query: 181 QSAVASFKTLLKQAQPDVEFVAEQAPPLGKIDAGAVAQALVDAKPDAIFNALFAADLGKF 240 QSAVASFKTL+KQAQPDVEFVAEQAPPLGKIDAGAV QAL DAKPDAIFNALFAADLGKF Sbjct: 181 QSAVASFKTLMKQAQPDVEFVAEQAPPLGKIDAGAVTQALADAKPDAIFNALFAADLGKF 240 Query: 241 VREGNTRGLFDQVEVVSLLTGEPEYLDPLKNEAPQNWIVTGYPWYAINTPEHKKFVDAYR 300 VREGNTRGLF+QV+VVSLLTGEPEYLDPLK EAPQNWIVTGYPWYAI+TPEHKKFV+AYR Sbjct: 241 VREGNTRGLFEQVDVVSLLTGEPEYLDPLKGEAPQNWIVTGYPWYAIHTPEHKKFVEAYR 300 Query: 301 KKFNDYPRNGSLVGYSALMSIAAGIKKAGSTDADKLAAAFSGLKVETPVASIVYRPQDHQ 360 KKFNDYPRNGSLVGYSALMSIAAG++KAGSTDAD+LAAAFSGLKVETPVASIVYRPQDHQ Sbjct: 301 KKFNDYPRNGSLVGYSALMSIAAGLRKAGSTDADRLAAAFSGLKVETPVASIVYRPQDHQ 360 Query: 361 STLGAFVGRTGIKDGKGIMTSFSYVDGASLQLPDAEVKKLRPAD 404 STLGAFVGRTG+K+G+G+MTSF+Y+DGA+LQLPDAEVKKLR D Sbjct: 361 STLGAFVGRTGLKEGRGVMTSFTYIDGAALQLPDAEVKKLRAPD 404 >gi|300310957|ref|YP_003775049.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 413 Score = 352 bits (902), Expect = 4e-98, Method: Compositional matrix adjust. Identities = 171/380 (45%), Positives = 255/380 (67%) Query: 24 GAAQAQETIKIGEINSYKAQPAFLEPYRRGWEMALDEVNAAGGVLGKKLEVIARDDNGNP 83 G + A E I+IGEINS + YR G +AL+++NAAGGV G+ L+++ R+D+G+ Sbjct: 30 GPSHAAEAIRIGEINSRHRLSTAGQAYRNGLALALEQINAAGGVGGRPLQLVVREDDGDA 89 Query: 84 GDSVRVAEELMARERVALLFGGFLSNTGLALTDYAKQHKVFFLAAEPLTDKIVWQNGNKY 143 + R A L++++ V +L G LS+ LA++ A + + + + L+D I GN+Y Sbjct: 90 DQAARNARALISQDHVEVLTGSMLSDVALAVSREAARGRTVLVVGDALSDAITLDKGNRY 149 Query: 144 TYRLRPSTYMLVASVVKEAAKLKKKRWAIVYPNYEYGQSAVASFKTLLKQAQPDVEFVAE 203 T+R+RPSTYM A + ++AA+L +RWA+V PNYEYGQSAVASFK LLKQA+PDVEFV++ Sbjct: 150 TFRVRPSTYMQAAMLAEQAARLPGRRWAVVAPNYEYGQSAVASFKLLLKQARPDVEFVSD 209 Query: 204 QAPPLGKIDAGAVAQALVDAKPDAIFNALFAADLGKFVREGNTRGLFDQVEVVSLLTGEP 263 Q P LGKI A VA+A+ ++PD IFNA F DL KFV+ GN + LF+ V VVS+ + Sbjct: 210 QWPALGKIKADDVARAIRQSRPDGIFNATFGEDLQKFVQAGNRQQLFEGVRVVSMASAGA 269 Query: 264 EYLDPLKNEAPQNWIVTGYPWYAINTPEHKKFVDAYRKKFNDYPRNGSLVGYSALMSIAA 323 LDP ++EA ++W+ GYPW ++ PEH++F++AY K+F++ P+ ++ GY+ +M+IAA Sbjct: 270 PSLDPSQSEAIRDWLAIGYPWDQVSLPEHQRFLEAYLKRFSERPQLSAVFGYTTMMAIAA 329 Query: 324 GIKKAGSTDADKLAAAFSGLKVETPVASIVYRPQDHQSTLGAFVGRTGIKDGKGIMTSFS 383 G+KK+ D + L A GL+ +P+ + +R D Q++LGAFVG KDG+ M + Sbjct: 330 GLKKSEGGDGEVLVEAMRGLQFGSPLGPVSFRALDQQASLGAFVGLVSQKDGRSSMRDWR 389 Query: 384 YVDGASLQLPDAEVKKLRPA 403 Y+DGA+ P A V+ RPA Sbjct: 390 YLDGAAYLPPPAYVRSRRPA 409 >gi|300312236|ref|YP_003776328.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 87.8 bits (216), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 86/324 (26%), Positives = 143/324 (44%), Gaps = 37/324 (11%) Query: 51 RRGWEMALDEVNAAGGVLGKKLEVIARDDNGNPGDSVRVAEELMARERVALLFGGFLSNT 110 + G+ +A +E+NA GGV G KL ++ D+ G +++ V ++L+ +++V +FG LSN+ Sbjct: 48 KNGFTLASEEINAKGGVNGDKLALVIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNS 107 Query: 111 GLALTDYAKQHKVFFLAAEPLTDKIVWQNGNKYTYRLRPSTYMLVASVVKEAAK-LKKKR 169 A A KV D I +T+R ++ VK A K K+ Sbjct: 108 AFAADPIANAAKVVVFGTSNTADGITAM--GPFTFRNSVMEADVLPVTVKAAVKHFGIKK 165 Query: 170 WAIVYPNYE-YGQSAVASFKTLLKQ-----------AQPDVEFVAEQAPPLGKIDAGAVA 217 A++Y N + + +S FK L+ A+ DV+F A+ L KI AG Sbjct: 166 VAVIYGNDDAFTKSGYDVFKATLEHQKIPVTTTEAYAKGDVDFKAQ----LTKIKAG--- 218 Query: 218 QALVDAKPDAIFNALFAADLGKFVREGNTRGLFDQVEVVSLLTGEPEYLDPLKNEAPQNW 277 PDAI + A + + + G+ Q + P+ + + +A N Sbjct: 219 ------NPDAIVCSCLAEEAANIILQTRALGM-KQPFIGGNGLNSPKLFE-IAKDAGDNT 270 Query: 278 IVTGYPWYAIN-TPEHKKFVDAYRKKFNDYPRNGSLVGYSALMSIAAGIKKAG-----ST 331 ++ G PW A N TP +K F+ AY+ KF P + Y A+ +A +K + Sbjct: 271 LM-GSPWSAENQTPANKAFITAYKAKFGADPDQFAAQAYDAMYIVADALKNVKLSGNLAK 329 Query: 332 DADKLAAAFSGLKVETPVASIVYR 355 D D L AA +K++ +R Sbjct: 330 DRDALRAALPAVKIDGATGKFAFR 353 >gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 85.1 bits (209), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 83/314 (26%), Positives = 131/314 (41%), Gaps = 17/314 (5%) Query: 53 GWEMALDEVNAAGGVLGKKLEVIARDDNGNPGDSVRVAEELMARERVALLF-GGFLSNTG 111 G ++AL + G VLGK + D G P +VR +E + E+ F GG LS Sbjct: 53 GMKLALQKY---GKVLGKSTSYVNLDTEGKPATAVRKMQESI--EKGTRFFAGGILSGEA 107 Query: 112 LALTDYAKQHKVFFLAAEPLTDKIVWQNGNKYTYRLRPSTYMLVASVVKEAAKLKK---- 167 LA+ A + F+ D+I + N+ T+R T+ + V+ L+K Sbjct: 108 LAMGKEADKAGAVFITTAG-ADEITGSDCNRATFRWSVPTFGAIEQTVRPL--LEKMPNA 164 Query: 168 KRWAIVYPNYEYGQSAVASFKTLLKQAQPDVEFVAEQAPPLGKIDAGAVAQALVDAKPDA 227 KRW + P Y +G+ +++ K + K+ +E V L + + + AKPD Sbjct: 165 KRWYTITPQYVFGEGLLSAAKNIFKE--KGIEHVGNSYHSLTEKEFSGYLTNAIAAKPDV 222 Query: 228 IFNALFAADLGKFVREGNTRGLFDQVEVVSLLTGEPEYLDPLKNEAPQNWIVTGYPWYAI 287 + F + +R+ + G+ + ++ E + L + W+AI Sbjct: 223 LLLLNFGSQSSDTLRQAVSFGMKNNCTILMAWASGLEQFETLGADICDGVYFGAQYWHAI 282 Query: 288 NTPEHKKFVDAYRKKFNDYPRNGSLVG-YSALMSIAAGIKKAGSTDADKLAAAFSGLKVE 346 + P +K+FV P N SL G Y I I KA STD + AA G+K E Sbjct: 283 DAPFNKEFVKLVNDNLKIDP-NYSLAGSYICTKIILDAIIKANSTDPKAVIAAMEGMKYE 341 Query: 347 TPVASIVYRPQDHQ 360 S R DHQ Sbjct: 342 GLTGSEEIRKGDHQ 355 >gi|300313292|ref|YP_003777384.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 391 Score = 78.2 bits (191), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 97/362 (26%), Positives = 159/362 (43%), Gaps = 17/362 (4%) Query: 4 FNFQRGLLWACAATIVNLSAGAAQAQETIKIGEINSYKAQPAFLEPYRRGWEMALDEVNA 63 F++ L A A L A AQAQETIK+G I ++ Y + E + A Sbjct: 1 MRFKKIALLAVMAASGGLLASLAQAQETIKVGLIAAFSGP---FADYGKQMEGGIKAYMA 57 Query: 64 AGG--VLGKKLEVIARDDNG-NPGDSVRVAEELMARERVALLFGGFLSNTGLALTDYAKQ 120 G V GKK+++I +D G +P + R+A+EL+ RE+V L G L+ LA+ A+Q Sbjct: 58 QHGDQVAGKKIQIIIKDTTGPSPEIAKRLAQELVVREKVDFLAGFGLTPEALAVAPIAEQ 117 Query: 121 HKVFFLAAEPLTDKIVWQNGNKYTYRLRPSTYMLVASVVKEAAKLKKKRWAIVYPNYEYG 180 K + T I + + Y R + + + A K KR + +Y G Sbjct: 118 AKKPMIIMNAATSVITTK--SNYIARFSMTLPQVSGPMATWALKNGIKRVVTLVADYGPG 175 Query: 181 QSAVASFKT-LLKQAQPDVEFVAEQAPPLGKIDAGAVAQALVDAKPDAIFNALFAADLG- 238 A +FKT LL +E + PL + Q + DAKP+A+F + A + Sbjct: 176 IDAETAFKTNLLGGGGQVLESIRV---PLRNPEFAPYIQRIKDAKPEAVFIFVPAGEQSI 232 Query: 239 KFVREGNTRGLFDQ-VEVVSLLTGEPEYLDPLKNEAPQNWIVTGYPWYAINTPEHKKFVD 297 F++ RGL + ++V++ +++ P E+ I T + A ++PE+K F+ Sbjct: 233 AFMKGYRERGLAEAGIKVIATGDLTDDHVMPAMGESTLGVITTFHYSAAHDSPENKAFLK 292 Query: 298 AY--RKKFNDYPRNGSLVGYSALMSIAAGIKKA-GSTDADKLAAAFSGLKVETPVASIVY 354 ++ P ++ Y + +I KK G D D+ A +K +P I Sbjct: 293 SFAAANPGAGRPNFMAVAAYDGMNAIYEVSKKLNGKIDGDRAMAILKTMKFTSPRGPIAI 352 Query: 355 RP 356 P Sbjct: 353 DP 354 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.134 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,314,266 Number of extensions: 56592 Number of successful extensions: 223 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 211 Number of HSP's successfully gapped: 19 Length of query: 404 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 317 Effective length of database: 1,207,925 Effective search space: 382912225 Effective search space used: 382912225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)