BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0071.p (509 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311771|ref|YP_003775863.1| branched-chain amino acid ABC t... 754 0.0 gi|300312954|ref|YP_003777046.1| amino acid ABC transporter perm... 164 2e-41 gi|300313932|ref|YP_003778024.1| branched-chain amino acid ABC t... 150 2e-37 gi|300314070|ref|YP_003778162.1| branched-chain amino acid ABC t... 134 1e-32 gi|300311946|ref|YP_003776038.1| branched-chain amino acid ABC t... 112 7e-26 >gi|300311771|ref|YP_003775863.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 628 Score = 754 bits (1946), Expect = 0.0, Method: Compositional matrix adjust. Identities = 438/509 (86%), Positives = 467/509 (91%) Query: 1 MTLASLTVQLLNGLADASAMFLVAAGLSLIFGVTRIVNFAHGSFYMLGLYVAYSLVERLG 60 MTLASL VQLLNGLADASAMF+VAAGLSLIFGVTRIVNFAHGSFYMLGLY+AYSLV+RLG Sbjct: 1 MTLASLAVQLLNGLADASAMFMVAAGLSLIFGVTRIVNFAHGSFYMLGLYLAYSLVDRLG 60 Query: 61 STALGFWGALLLAALTVAALGAVVEVLVLRRIYRAPELFQLLATFALVLIIKDAALWIWG 120 +T G+W ALLLAAL VAALGA+VE+ +LRRIYRAPELFQLLATFALVL+IKDAALW WG Sbjct: 61 ATPWGYWAALLLAALPVAALGALVELTILRRIYRAPELFQLLATFALVLVIKDAALWYWG 120 Query: 121 PEDLFGPRAPGLSGAVHLLGRRLPQYDLLLIVIGPLVFGLLWLLLNRTRWGTLIRAATQD 180 PEDLFGPRAPGL+GAV +LGRRLPQYDLLL+ IGPLVF LLWLLLNRTRWG LIRAATQD Sbjct: 121 PEDLFGPRAPGLAGAVQVLGRRLPQYDLLLMAIGPLVFALLWLLLNRTRWGALIRAATQD 180 Query: 181 REMLGALGVNQAWLFTAVFALGCFLAGLGGALQGPRMPANLALDLDTIGNAFVVVVVGGM 240 REMLGALGVNQAWLFT VFALGCFLAGLGGALQGPR+PANLALDLDTIG AFVVVVVGGM Sbjct: 181 REMLGALGVNQAWLFTGVFALGCFLAGLGGALQGPRLPANLALDLDTIGTAFVVVVVGGM 240 Query: 241 GSLGGAFAAALLIAEVKALCIALGNVSLFGIEVSLSRLTLVVEFLVMAAILVMRPWGLFG 300 GS+GGAFAAA+LIAEVKALCIALG VS FGIE+SLSRLTLV EFLVMA ILV+RPWGL G Sbjct: 241 GSIGGAFAAAVLIAEVKALCIALGTVSCFGIELSLSRLTLVAEFLVMAVILVVRPWGLLG 300 Query: 301 KPLALVRSGAGHDAPLRAPGRKARLAGWAALLALLALPLLASRFPYLPVLMVEILIAVLF 360 KPLALVR+ + H+APLRA ++ R A L ALLALPLLA RFPYLPVLMVEIL+AVLF Sbjct: 301 KPLALVRAASHHEAPLRAASQRERWAAMVLLAALLALPLLAGRFPYLPVLMVEILVAVLF 360 Query: 361 AASLHFLMGPGGMVSFGHAAYFGLGAYAAALVTLKLQWPMPLAMLAGALAAMAGAILFGW 420 AASLHFLMGPGGMVSFGHAAYFGLGAYAAALVTL+LQWPMPLAMLAGA AM A+LFGW Sbjct: 361 AASLHFLMGPGGMVSFGHAAYFGLGAYAAALVTLRLQWPMPLAMLAGAGTAMLAALLFGW 420 Query: 421 FCVRLSGVYLAMLTLAFAQIVWATFFQWDDLTGGSNGLVGIRPAETLASPLAYYYLALGC 480 FCVRLSGVYLAMLTLAFAQIVWA +QWDDLTGGSNGLVG+R A LASPLAYYYLAL C Sbjct: 421 FCVRLSGVYLAMLTLAFAQIVWAAIYQWDDLTGGSNGLVGLRTAAALASPLAYYYLALLC 480 Query: 481 AALGVILLRRVIHAPFGLALRAARDAAVR 509 +ALGV LLRRVIHAPFGLALRAARDA+ R Sbjct: 481 SALGVYLLRRVIHAPFGLALRAARDASGR 509 >gi|300312954|ref|YP_003777046.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 304 Score = 164 bits (414), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 116/298 (38%), Positives = 166/298 (55%), Gaps = 12/298 (4%) Query: 9 QLLNGLADASAMFLVAAGLSLIFGVTRIVNFAHGSFYMLGLYVAYSLVERLGSTALGFWG 68 Q+LN L +FL+A+GL+LIFGV VN AHGSF+M + + + + FW Sbjct: 8 QVLNSLQFGVMLFLIASGLTLIFGVMNFVNLAHGSFFM----IGAYVAAVVAGASGSFWL 63 Query: 69 ALLLAALTVAALGAVVEVLVLRRIYRAPELFQLLATFALVLIIKDAALWIWGPEDLFGPR 128 LL+A AALG ++E +V RR+Y+ + Q+LATFA +LI D IWGP+ L Sbjct: 64 GLLVALPATAALGFLLEWVVFRRLYQKDHMVQVLATFAFILIFNDGVRMIWGPQPLPLNM 123 Query: 129 APGLSGAVHLLGRRLPQYDLLLIVIGPLVFGLLWLLLNRTRWGTLIRAATQDREMLGALG 188 +G V+L G Y LL++ +G +V L+ L+NRTR G +RA + EM+ A+G Sbjct: 124 PAAFAGPVNLFGLPYSSYRLLVMAVGVVVAIALYFLINRTRIGMWVRAGANNLEMVTAMG 183 Query: 189 VNQAWLFTAVFALGCFLAGLGGALQGPRMPANLALDLDTIGNAFVVVVVGGMGSLGGAFA 248 +N LF VF+ G LA L G + GP + + D + AFVVVV+GG+GS+ GA Sbjct: 184 INIRLLFRLVFSFGLLLAALAGIMLGPLVAVQAGMGDDVLILAFVVVVIGGIGSVPGAAI 243 Query: 249 AALLIAEVKALCIALGNVSLFGIEVSLS-------RLTLVVEFLVMAAILVMRPWGLF 299 AL++ V L +L LF + +S L ++ +LVM A+L RP GLF Sbjct: 244 GALIVGVVDTLGRSLLP-QLFALFLSADAAASVGPALGSMMIYLVMIAVLFWRPSGLF 300 >gi|300313932|ref|YP_003778024.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 288 Score = 150 bits (379), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 110/296 (37%), Positives = 171/296 (57%), Gaps = 19/296 (6%) Query: 9 QLLNGLADASAMFLVAAGLSLIFGVTRIVNFAHGSFYMLGLYVAYSLVERLGSTALGFWG 68 Q+ NG+ FL+A GLS++FG+ R VNF+HG+F++LG Y+ + + + LGFW Sbjct: 7 QIANGVGIGMLYFLLAVGLSIVFGLLRFVNFSHGAFFLLGAYLCFQVTD----LGLGFWW 62 Query: 69 ALLLAALTVAALGAVVEVLVLRRIYRAPELFQLLATFALVLIIKDAALWIWGPEDLFGPR 128 +LL+A L V +VE VLR +Y P F +L T + L+I++A + WGP L Sbjct: 63 SLLIAPLVVGLFAWIVERFVLRHVYALPHHFHILFTVGMALVIQEAVISTWGP--LGNNV 120 Query: 129 APG--LSGAVHLLGRRLPQYDLLLIVIGPLVFGLLWLLLNRTRWGTLIRAATQDREMLGA 186 AP L G V G P+Y L +I + ++ LLW +L TR G+++RA ++ EM+ Sbjct: 121 APPDLLQGVVMWGGFVYPKYRLFVIGLTAVMALLLWWVLEGTRLGSIVRAGSESTEMVSL 180 Query: 187 LGVNQAWLFTAVFALGCFLAGLGGALQGPRMPANLALDLDTIGNAFVVVVVGGMGSLGGA 246 LG+N +F+ VFALG AGL GAL P A+ + ++ +G AFVVVV+GG+GS GA Sbjct: 181 LGLNINRIFSLVFALGAATAGLAGALAAPIRGADPFMGVEALGIAFVVVVIGGLGSFWGA 240 Query: 247 FAAALLIAEVKALCIALGNVSLFGIEVSLSRLTLVVEFLVMAAILVMRPWGLFGKP 302 +L+ V+++ + S ++ ++ MAA++++RP GL G+P Sbjct: 241 LIGGILVGIVQSV-----------MSTVWSEGAHLMIYVAMAAVMLLRPNGLLGRP 285 >gi|300314070|ref|YP_003778162.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 294 Score = 134 bits (338), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 92/286 (32%), Positives = 152/286 (53%), Gaps = 14/286 (4%) Query: 16 DASAMFLVAAGLSLIFGVTRIVNFAHGSFYMLGLYVAYSLVERLGSTALGFWGALLLAAL 75 + S +++ GL++IFG+ ++NFAHG+ YMLG ++A+ + LG L +W L++A + Sbjct: 21 NGSFYAMLSLGLAVIFGLLNVINFAHGTLYMLGAFLAWMGLSYLG---LNYWVMLIVAPI 77 Query: 76 TVAALGAVVEVLVLRRIYRAPELFQLLATFALVLIIKDAALWIWGPEDLFGPRAPGLSGA 135 V A G V+E +LR +Y+ L+ LL TF + L+I+ I+G L GA Sbjct: 78 LVGAFGIVIEKTMLRWLYKLDHLYGLLLTFGITLMIEGIFRSIYGVSGQPYSVPDLLQGA 137 Query: 136 VHLLGRRLPQYDLLLIVIGPLVFGLLWLLLNRTRWGTLIRAATQDREMLGALGVNQAWLF 195 +L LP Y ++V +V W ++ +TR G +RA T++ +++ A G+N + Sbjct: 138 TNLGFMVLPNYRAWVVVASLIVCFATWFVIEKTRLGAYLRAGTENPKLVEAFGINVPLMV 197 Query: 196 TAVFALGCFLAGLGGALQGPRMPANLALDLDTIGNAFVVVVVGGMGSLGGAFAAALLIAE 255 T + G LA G L P + + + + I F VVV+GGMGS+ G+ L++ Sbjct: 198 TLTYGFGVALAAFAGVLAAPVIQVSPLMGSNLIITVFAVVVIGGMGSILGSIITGLVLGL 257 Query: 256 VKALCIALGNVSLFGIEVSLSRLTLVVEFLVMAAILVMRPWGLFGK 301 ++ L V L+ V F++MA +L++RP GLFGK Sbjct: 258 IEGLT-----------RVFYPELSATVVFIIMAIVLMVRPAGLFGK 292 >gi|300311946|ref|YP_003776038.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 293 Score = 112 bits (280), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 100/303 (33%), Positives = 162/303 (53%), Gaps = 27/303 (8%) Query: 8 VQLLNGLADASAMFLVAAGLSLIFGVTRIVNFAHGSFYMLGLYVAYSLVERLGST----- 62 +Q NGL+ + L+A GL++IFG ++N AHG F +G Y+ Y L+ L +T Sbjct: 1 MQGFNGLSVFCVLLLMALGLAIIFGQMGVINMAHGEFLTIGAYITY-LLSHLTTTYAPAL 59 Query: 63 -ALGFWGALLLAALTVAALGAVVEVLVLRRIYRAPELFQLLATFALVLIIKDAALWIWGP 121 F+ A++L+ +G + E+L++ R+Y+ P L LLAT+ L L ++ I+G Sbjct: 60 QPFYFFVAIVLSFAVAYGVGYLTELLLISRLYKRP-LDTLLATWGLSLAMQQTFRSIFGA 118 Query: 122 EDLFGPRAPGLSGAVHLLGR-RLPQYDLLLIVIGPLVFGLLWLLLNRTRWGTLIRAATQD 180 +++ L G+ +P L ++ + L+ G +++LL R+RWG +RA Q+ Sbjct: 119 KEVSATLPDWLMGSFKPTDDIDIPLNGLFMMGLTVLLTGAIFVLLFRSRWGLQVRATMQN 178 Query: 181 REMLGALGVNQAWLFTAVFALGCFLAGLGGA---LQGPRMPANLALDLDTIGNAFVVVVV 237 R M GA+G+N + FALGC +AG+ GA G P + +L I + F+VVV Sbjct: 179 RVMSGAVGINTRKVDRTTFALGCGVAGVAGAAFTTIGSTGPTSGSL---YIVDTFLVVVF 235 Query: 238 GGMGSLGGAFAAALLIAEVKALCIALGNVSLFGIEVSLSR-LTLVVEFLVMAAILVMRPW 296 GG SL G A+A IA+ + + + F + S+++ LTL L IL++RP Sbjct: 236 GGAQSLIGTIASAFSIAQTQ-------STAEFFLTGSMAKVLTLSAVIL----ILMLRPQ 284 Query: 297 GLF 299 GLF Sbjct: 285 GLF 287 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.144 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,130,854 Number of extensions: 36528 Number of successful extensions: 285 Number of sequences better than 1.0e-08: 29 Number of HSP's gapped: 259 Number of HSP's successfully gapped: 31 Length of query: 509 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 420 Effective length of database: 1,198,455 Effective search space: 503351100 Effective search space used: 503351100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 133 (55.8 bits)