BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0034.0069.p
         (172 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312605|ref|YP_003776697.1| FAD/FMN-containing dehydrogenas...   168   2e-43
gi|300313123|ref|YP_003777215.1| D-lactate dehydrogenase [Herbas...    68   5e-13
gi|300313121|ref|YP_003777213.1| glycolate oxidase (FAD-linked s...    57   8e-10

>gi|300312605|ref|YP_003776697.1| FAD/FMN-containing dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 475

 Score =  168 bits (425), Expect = 2e-43,   Method: Compositional matrix adjust.
 Identities = 82/172 (47%), Positives = 114/172 (66%)

Query: 1   MLEELLGQAMEDALVTDAMLCSNGSQAAALWKIREGISQAQVRAGKVIKHDIALPISAIA 60
           + E L+G A+E  LV DA++ ++ +Q+ ALW++RE IS AQ   GK IKHDI++PIS IA
Sbjct: 304 LFEGLIGTALEQGLVDDAVIAASLAQSRALWRLRESISSAQAHEGKNIKHDISVPISRIA 363

Query: 61  EFVDQAERAIAACGLQAEIINFGHLGDGNLHFNVMIPLSSSYEEVRQATLLLNRLVHDLV 120
           EF+   +  +        ++ FGHLGDGNLH+NV  P   +  +       +NR+VHD V
Sbjct: 364 EFIAATDVLVQQAAPGCRMVTFGHLGDGNLHYNVSPPEGVADRDFIARQAAINRVVHDSV 423

Query: 121 TEKQGSISAEHGVGQLRRDELRHYKSPLEMELMLRIKQAFDPNLIMNPGKLL 172
               GSISAEHG+G L+R+E+  YKSP+E++LM  IK A DP  +MNPGK+L
Sbjct: 424 DRFGGSISAEHGLGALKREEITRYKSPVELQLMRAIKLALDPQQLMNPGKVL 475


>gi|300313123|ref|YP_003777215.1| D-lactate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 471

 Score = 67.8 bits (164), Expect = 5e-13,   Method: Compositional matrix adjust.
 Identities = 51/146 (34%), Positives = 72/146 (49%), Gaps = 7/146 (4%)

Query: 30  LWKIREGISQA--QVRAG-KVIKHDIALPISAIAEFVDQAERAIAACGLQAEIINFGHLG 86
           LW  R     A  Q+R G + I  D  +PIS +AE V   +      GL   II  GH+G
Sbjct: 326 LWTARHNAYFALLQLRPGARAISTDCCVPISRLAECVLATKADCEEQGLTHAII--GHVG 383

Query: 87  DGNLHFNVMIPLSSSYEEVRQATLLLNRLVHDLVTEKQGSISAEHGVGQLRRDELRHYKS 146
           DGN H  +M+   +   ++ +A  +  R+V   +    G+ + EHGVG  + D L     
Sbjct: 384 DGNFHVQMMVD-PNDPADIARAEGVNQRMVARAIG-MDGTCTGEHGVGLHKMDFLIQEHG 441

Query: 147 PLEMELMLRIKQAFDPNLIMNPGKLL 172
              + +M  IK A DP  IMNPGK+L
Sbjct: 442 EGAIAVMRAIKHALDPKNIMNPGKIL 467


>gi|300313121|ref|YP_003777213.1| glycolate oxidase (FAD-linked
           subunit) oxidoreductase [Herbaspirillum seropedicae
           SmR1]
          Length = 495

 Score = 57.0 bits (136), Expect = 8e-10,   Method: Compositional matrix adjust.
 Identities = 32/92 (34%), Positives = 52/92 (56%), Gaps = 2/92 (2%)

Query: 81  NFGHLGDGNLHFNVMIPLSSSYEEVRQATLLLNRLVHDLVTEKQGSISAEHGVGQLRRDE 140
           N  H GDGNLH  ++   + + +E  +A L    ++ +L  E  G+I+ EHGVG  + + 
Sbjct: 379 NVFHAGDGNLHPLILFDANIA-DEFHRAELFGAEIL-ELCVEVGGTITGEHGVGIEKINS 436

Query: 141 LRHYKSPLEMELMLRIKQAFDPNLIMNPGKLL 172
           +    SP E E   ++K+AFDP  ++NP K +
Sbjct: 437 MCVQFSPAERETFFKLKRAFDPAFLLNPDKAI 468


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.135    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 515,479
Number of extensions: 19275
Number of successful extensions: 65
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 64
Number of HSP's successfully gapped: 3
Length of query: 172
Length of database: 1,619,870
Length adjustment: 79
Effective length of query: 93
Effective length of database: 1,245,805
Effective search space: 115859865
Effective search space used: 115859865
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 127 (53.5 bits)