BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0034.0066.p
         (162 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312605|ref|YP_003776697.1| FAD/FMN-containing dehydrogenas...   118   2e-28
gi|300313121|ref|YP_003777213.1| glycolate oxidase (FAD-linked s...    68   3e-13
gi|300313341|ref|YP_003777433.1| FAD-dependent D-lactate dehydro...    61   3e-11

>gi|300312605|ref|YP_003776697.1| FAD/FMN-containing dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 475

 Score =  118 bits (295), Expect = 2e-28,   Method: Compositional matrix adjust.
 Identities = 59/126 (46%), Positives = 82/126 (65%), Gaps = 4/126 (3%)

Query: 1   MNENTQQLIEQLRSIVGDAGLVTAADEQAPYVKDWLNKWQGRVAVVVRPADTAQTAEVVR 60
           MNE     ++  R+ +G   ++T A + A Y+ D   ++ GR   V+RPAD+A+ A VVR
Sbjct: 8   MNE----FLQACRAAIGAPHVLTDAHDTAGYLTDQRGRYTGRALAVLRPADSAEVAAVVR 63

Query: 61  LCHQTHTPIVTQGGNTGMSGGATPDDSGAQVILSTTRMNRIRAVDPINNTMTVDAGVILA 120
           LC Q   P+V QGGNTG+  G+ PD  G  V+LS  R+NRIRAVD +NNT+TV++G +L 
Sbjct: 64  LCAQFAVPLVPQGGNTGLVLGSVPDQQGNAVVLSLRRLNRIRAVDALNNTITVESGCVLQ 123

Query: 121 HAQEAA 126
           H QE A
Sbjct: 124 HLQEQA 129


>gi|300313121|ref|YP_003777213.1| glycolate oxidase (FAD-linked
           subunit) oxidoreductase [Herbaspirillum seropedicae
           SmR1]
          Length = 495

 Score = 68.2 bits (165), Expect = 3e-13,   Method: Compositional matrix adjust.
 Identities = 42/124 (33%), Positives = 68/124 (54%), Gaps = 3/124 (2%)

Query: 6   QQLIEQLRSIVGDAGLVTAADEQAPYVKDWLNKWQGRVAVVVRPADTAQTAEVVRLCHQT 65
           +++++ LR +V    ++   ++  PY  D L  ++    VVV P + AQ   +++ CH  
Sbjct: 15  REVVDALRRVVPAHCVLCDEEDTRPYECDGLAAYRQLPMVVVLPENEAQVVAIMKTCHAL 74

Query: 66  HTPIVTQGGNTGMSGGATPDDSGAQVILSTTRMNRIRAVDPINNTMTVDAGVI-LAHAQE 124
              IV +G  TG+SGGATP   G  V++ST + N+I  +D  + T  V  GV  LA ++ 
Sbjct: 75  QVQIVPRGAGTGLSGGATPIADG--VVVSTAKFNQILQMDKYSRTAVVQPGVRNLAISEA 132

Query: 125 AARH 128
           AA H
Sbjct: 133 AAPH 136


>gi|300313341|ref|YP_003777433.1| FAD-dependent D-lactate
           dehydrogenase [Herbaspirillum seropedicae SmR1]
          Length = 587

 Score = 61.2 bits (147), Expect = 3e-11,   Method: Compositional matrix adjust.
 Identities = 35/108 (32%), Positives = 56/108 (51%), Gaps = 4/108 (3%)

Query: 1   MNENTQQLIEQLRSIVGDAGLVTAADEQAPYVKDWLNKWQGRVAVVVRPADTAQTAEVVR 60
           M   T  +I +L +IVG   ++T  D Q    +  L    G V  VVRP    +   V++
Sbjct: 7   MERRTMDVISKLEAIVGSKHVLTG-DAQTRRFRQGLRYGGGEVLAVVRPGRLLEMWRVLQ 65

Query: 61  LCHQTHTPIVTQGGNTGMSGGATPDDSGAQ---VILSTTRMNRIRAVD 105
           +CH  +  I+ Q  NTG++GG+TP   G     V+++T R+ R+  +D
Sbjct: 66  VCHAANMVIICQAANTGLTGGSTPIAEGYDRPVVLINTMRLTRLELLD 113


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.313    0.128    0.371 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 552,518
Number of extensions: 21417
Number of successful extensions: 68
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 68
Number of HSP's successfully gapped: 3
Length of query: 162
Length of database: 1,619,870
Length adjustment: 78
Effective length of query: 84
Effective length of database: 1,250,540
Effective search space: 105045360
Effective search space used: 105045360
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 127 (53.5 bits)