BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0063.p (546 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine... 973 0.0 gi|300311805|ref|YP_003775897.1| two component hybrid sensor his... 325 6e-90 gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine k... 293 2e-80 gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine... 286 3e-78 gi|300310177|ref|YP_003774269.1| C4-dicarboxylate transport sens... 103 4e-23 >gi|300311768|ref|YP_003775860.1| PAS/PAC sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 552 Score = 973 bits (2516), Expect = 0.0, Method: Compositional matrix adjust. Identities = 468/533 (87%), Positives = 502/533 (94%) Query: 14 ADDFNDAGMRNDHAKVAVGDDANDIFFAAVEMTRMPMIVSDPNKPDNPIIFVNNAFMNMT 73 +D + DAG+RND AKVAVG+D NDIFFAAVEMTRMPMIVSDPN+PDNPI+FVNNAF+NMT Sbjct: 20 SDRYTDAGVRNDLAKVAVGNDINDIFFAAVEMTRMPMIVSDPNRPDNPIVFVNNAFINMT 79 Query: 74 GYSRAEVVGKNCRFLQGPETDRAVVAQVRQAVAEKRELATELLNYRKNGSTFWNALFISP 133 GYS AEVVGKNCRFLQGPETDR+VVA+VR+AVAE+RE+ATELLNYRKNGSTFWNALFISP Sbjct: 80 GYSSAEVVGKNCRFLQGPETDRSVVAEVRRAVAERREIATELLNYRKNGSTFWNALFISP 139 Query: 134 VYDQHGELKYFFSSQLDISRRRDAEQALGQARKMEALGQLTGGIAHDFNNLLQVISGYLD 193 VYDQ G LKYFFSSQLDISRRRDAEQALGQARKMEALGQLTGGIAHDFNNLLQVI+GYLD Sbjct: 140 VYDQQGNLKYFFSSQLDISRRRDAEQALGQARKMEALGQLTGGIAHDFNNLLQVITGYLD 199 Query: 194 IINLALRSDTPDLGRVSRSADSIRKASGKAAMLTQQLLAFARKQRLEGRTINLNSLTEGF 253 +INLALRSD PDL RV+RSADSIRKASGKAAMLTQQLLAFARKQRLEGRTINLNSLTEGF Sbjct: 200 MINLALRSDKPDLARVARSADSIRKASGKAAMLTQQLLAFARKQRLEGRTINLNSLTEGF 259 Query: 254 TDLVQRTLGGDIEVQTELASGLWNCRLDPTQMEVALLNVLINARDAMSGKGTVKIHTSNE 313 TDLV RTLGGDI VQTELA GLWNCRLDPTQMEVALLNVLINARDAM+G+G V+I T+NE Sbjct: 260 TDLVSRTLGGDITVQTELARGLWNCRLDPTQMEVALLNVLINARDAMNGRGRVRIRTANE 319 Query: 314 DLSSEDRAIQLGVKPGLYVCIAVTDNGPGIPADILPRVMDPFFTTKDEGKGTGLGLSMVY 373 DL+ DRA+QLG+K GLYV IAVTD+GPGI DILPRVMDPFFTTKDEGKGTGLGLSMVY Sbjct: 320 DLADPDRAVQLGLKEGLYVSIAVTDDGPGIAPDILPRVMDPFFTTKDEGKGTGLGLSMVY 379 Query: 374 GFVKQSGGSVRIVSSAERGTTVSIYFPATQDQVGGAFEYDNRTHTQGGHEHILVVDDRIE 433 GFVKQSGGSVRI S AE GTTV+IYFPATQDQVGG FEYDNR+ +QGG EHILVVDDR+E Sbjct: 380 GFVKQSGGSVRITSGAEGGTTVTIYFPATQDQVGGGFEYDNRSQSQGGQEHILVVDDRLE 439 Query: 434 VAELAQAMLESLGYRVSMVNSPTEALQVLRSEPKVDLLFTDLIMPGSMNGVVLAREARRM 493 VAELAQ MLESLGYRVSMVNSP EALQ++RS VDLLFTDLIMPGSMNGVVLAREA+RM Sbjct: 440 VAELAQTMLESLGYRVSMVNSPAEALQLIRSGRPVDLLFTDLIMPGSMNGVVLAREAKRM 499 Query: 494 IPSIKILLTTGYASESIERHGADGEFPVLDKPYRHDELARKIRIVLDGATGVS 546 +P+IK+LLTTGYASES+ERHGADGEFPVLDKPYRHDELARKIRIVLDGATGVS Sbjct: 500 LPTIKVLLTTGYASESLERHGADGEFPVLDKPYRHDELARKIRIVLDGATGVS 552 >gi|300311805|ref|YP_003775897.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1278 Score = 325 bits (833), Expect = 6e-90, Method: Compositional matrix adjust. Identities = 197/504 (39%), Positives = 280/504 (55%), Gaps = 37/504 (7%) Query: 65 VNNAFMNMTGYSRAEVVGKNCRFLQGPETDRAVVAQVRQAVAEKRELATELLNYRKNGST 124 VN A M G S E++G + P+ ++ + + + R E R +GST Sbjct: 596 VNQALCRMLGRSEEEMLGMTLADVTHPDDVPMNMSLFNRCIEDGRSFTLEKRYLRPDGST 655 Query: 125 FWNALFISPVYDQHGELKYFFSSQLDISRRRDAEQALG---------------------- 162 FW + IS + D G + + DI+ RR AE+AL Sbjct: 656 FWVSSDISRIVDGEGRPQAMIAVSTDITERRRAEEALRELNDTLEQRVVEEILERSRAEA 715 Query: 163 ---QARKMEALGQLTGGIAHDFNNLLQVISGYLDIINLALRSDTPDLGRVSRSADSIRKA 219 Q +KMEA+GQLTGG+AHDFNN+LQ+I G L ++ R D RS +R A Sbjct: 716 ALRQMQKMEAVGQLTGGVAHDFNNVLQIIGGNLQLLQSDPRLD-------ERSRQRVRIA 768 Query: 220 SG---KAAMLTQQLLAFARKQRLEGRTINLNSLTEGFTDLVQRTLGGDIEVQTELASGLW 276 G + A L+ QLLAFAR+Q L+ R+INL + + DL++R +G +I++ T +A LW Sbjct: 769 IGAVDRGAKLSSQLLAFARRQPLQPRSINLERVVQNMDDLLRRAVGENIDISTRVAHDLW 828 Query: 277 NCRLDPTQMEVALLNVLINARDAMSGKGTVKIHTSNEDLSSEDRAIQLGVKPGLYVCIAV 336 N LDP Q+E ++N INARDAM G + + N L Q + PG YV + + Sbjct: 829 NTMLDPNQVENLIINTAINARDAMPSGGRLTVQIDNVRLEDPVFLSQADLAPGEYVELQI 888 Query: 337 TDNGPGIPADILPRVMDPFFTTKDEGKGTGLGLSMVYGFVKQSGGSVRIVSSAERGTTVS 396 +DNG G+ ++ R +PFFTTK EG+GTGLGLSM YGFVKQS G + I S +GTT+ Sbjct: 889 SDNGAGMSPEVRDRAFEPFFTTKLEGEGTGLGLSMAYGFVKQSKGHISIHSEQGKGTTIR 948 Query: 397 IYFPATQDQVGGAFEYDNRTHTQGGHEHILVVDDRIEVAELAQAMLESLGYRVSMVNSPT 456 IYFP D+ E + T GG E ILVV+D + L SLGYRV ++ Sbjct: 949 IYFPRCLDEEVRIDEVRDLT-VHGGDETILVVEDDPNLQLTVIDTLSSLGYRVLKADNGE 1007 Query: 457 EALQVLRSEPKVDLLFTDLIMPGSMNGVVLAREARRMIPSIKILLTTGYASESIERHG-A 515 AL++L+S +DL+FTD++MPGS+ LA++AR ++P I+IL T+GY ++I G Sbjct: 1008 RALEILQSGVAIDLVFTDVVMPGSVPVTELAQQARLLVPDIEILFTSGYPRDTIVHEGRL 1067 Query: 516 DGEFPVLDKPYRHDELARKIRIVL 539 D +L KPYR ++LA++IR +L Sbjct: 1068 DAGVELLSKPYRINQLAQRIRQML 1091 Score = 61.6 bits (148), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 42/112 (37%), Positives = 61/112 (54%), Gaps = 4/112 (3%) Query: 420 GGHEHILVVDDRIEVAELAQAMLESLGYRVSMVNSPTEALQVLRSEPKVDLLFTDLIMPG 479 G +LVV+D I+ +L +LE+LG++ V S EALQ L E D+LFTD+ +PG Sbjct: 1161 AGGLRLLVVEDNIDSQQLVCELLETLGHQARGVISGEEALQAL-EEQGFDVLFTDVNLPG 1219 Query: 480 SMNGVVLAREARRMIPSIKILLTTGYASESIERHGADGEFPVLDKPYRHDEL 531 M+GV LA+ A P + ++ +GY + G L KPY D+L Sbjct: 1220 -MSGVELAKAALGRFPDLAVIFASGYGTMVARNVGFAAH--ALPKPYDIDQL 1268 >gi|300309882|ref|YP_003773974.1| multi-sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 845 Score = 293 bits (750), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 169/388 (43%), Positives = 241/388 (62%), Gaps = 9/388 (2%) Query: 158 EQALGQARKMEALGQLTGGIAHDFNNLLQVISGYLDIINLALRSDTPDLGRVSRSADSIR 217 E+AL ++K+EA+GQLTGG+AHDFNNLL ISG LD++ + L + + R + Sbjct: 456 EEALRHSQKIEAIGQLTGGLAHDFNNLLGGISGSLDLMRVRLVQGRGE--ELLRYVELAA 513 Query: 218 KASGKAAMLTQQLLAFARKQRLEGRTINLNSLTEGFTDLVQRTLGGDIEVQTELASGLWN 277 +++ +AA LTQ+LLAFAR+Q L + +N+L G +L++RT+G I ++ A GLW Sbjct: 514 RSTQRAASLTQRLLAFARRQTLAPKPTRVNALISGMEELIRRTIGPAIVLEVVGAGGLWP 573 Query: 278 CRLDPTQMEVALLNVLINARDAMSGKGTVKIHTSNEDLSSEDR-AIQLGVKPGLYVCIAV 336 +D Q+E ALLN+ INARDAM G G + I T+N+ L +DR A + + PG YVCI+V Sbjct: 574 TFVDGPQLESALLNLCINARDAMPGGGRLTIETANKWL--DDRWAHERDLPPGQYVCISV 631 Query: 337 TDNGPGIPADILPRVMDPFFTTKDEGKGTGLGLSMVYGFVKQSGGSVRIVSSAERGTTVS 396 TD+G G+P D+L R DPFFTTK G GTGLGLSMVYGF +QSGG VR+ S +GTTVS Sbjct: 632 TDSGVGMPPDVLARAFDPFFTTKPIGAGTGLGLSMVYGFARQSGGQVRLYSEVGQGTTVS 691 Query: 397 IYFPATQ---DQVGGAFEYDNRTHTQGGHEHILVVDDRIEVAELAQAMLESLGYRVSMVN 453 IY P Q D+ A D G E +LVVDD + L +L GY V Sbjct: 692 IYLPRHQHDGDEQDEADALDETAAGDGNGEVVLVVDDEASIRMLVAEILSDAGYDVLQAE 751 Query: 454 SPTEALQVLRSEPKVDLLFTDLIMPGSMNGVVLAREARRMIPSIKILLTTGYASESIERH 513 AL++L+S+ ++DLL TD+ +PG +NG +A R P + +L TGYA + + Sbjct: 752 DGPGALRILQSKQRIDLLITDVGLPGGLNGRQVADAGRAARPDLHVLFITGYAENAAFDN 811 Query: 514 GA-DGEFPVLDKPYRHDELARKIRIVLD 540 G + ++ KP++ + LAR+++ +L+ Sbjct: 812 GHLERGMQLMTKPFKMEALARRVQSILE 839 >gi|300311617|ref|YP_003775709.1| PAS/PAC sensor hybrid histidine kinase response regulator protein [Herbaspirillum seropedicae SmR1] Length = 688 Score = 286 bits (732), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 168/385 (43%), Positives = 234/385 (60%), Gaps = 7/385 (1%) Query: 156 DAEQALGQARKMEALGQLTGGIAHDFNNLLQVISGYLDIINLALRSDTPDLGRVSRSADS 215 +AE+AL QA+KMEA+GQLTGG+AHDFNN+L ISG L+++ + L +G + R + Sbjct: 296 NAEEALRQAQKMEAVGQLTGGLAHDFNNILAGISGSLELMKVRLAQGR--IGDIERHLNG 353 Query: 216 IRKASGKAAMLTQQLLAFARKQRLEGRTINLNSLTEGFTDLVQRTLGGDIEVQTELASGL 275 + A +AA LTQ+LLAF+R+Q L+ + +LN + G +L+ R++G I V+T A GL Sbjct: 354 AQSAVKRAAALTQRLLAFSRRQTLDPKPADLNRIVAGMHELIGRSVGPAIAVETIAAGGL 413 Query: 276 WNCRLDPTQMEVALLNVLINARDAMSGKGTVKIHTSNEDLSSEDRAIQLGVKPGLYVCIA 335 W+ +D QME ALLN+ INARDAM G + I T+N L + A+Q GV PG YV ++ Sbjct: 414 WHTFVDIGQMENALLNLCINARDAMPHGGRLTIETANRWLD-DIGALQRGVPPGQYVSLS 472 Query: 336 VTDNGPGIPADILPRVMDPFFTTKDEGKGTGLGLSMVYGFVKQSGGSVRIVSSAERGTTV 395 V+D G G+ +++ R DPFFTTK G+GTGLGLSMVYGF QSGG+VRI S +G V Sbjct: 473 VSDTGAGMSPEVVARAFDPFFTTKPTGQGTGLGLSMVYGFAGQSGGTVRIYSELGQGAMV 532 Query: 396 SIYFPATQDQVGG---AFEYDNRTHTQGGHEHILVVDDRIEVAELAQAMLESLGYRVSMV 452 IY P + G A ++ G + ILVVDD V + +L LGY V Sbjct: 533 CIYLPRHVGEGGVDDLALPEESHVPAAPGSKTILVVDDEPLVRMVTVEVLRDLGYSVLEA 592 Query: 453 NSPTEALQVLRSEPKVDLLFTDLIMPGSMNGVVLAREARRMIPSIKILLTTGYASESIER 512 AL+V+ + ++DLL TD+ +P MNG LA R P + +L TGYA ++ Sbjct: 593 EDGPSALRVVEACQEIDLLLTDVGLPNGMNGRQLADAIRAPRPDLPVLFVTGYAENAVLN 652 Query: 513 HG-ADGEFPVLDKPYRHDELARKIR 536 HG + VL KP+ D LAR+++ Sbjct: 653 HGHLERGMQVLTKPFAADVLARRVK 677 >gi|300310177|ref|YP_003774269.1| C4-dicarboxylate transport sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 674 Score = 103 bits (257), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 89/352 (25%), Positives = 160/352 (45%), Gaps = 23/352 (6%) Query: 62 IIFVNNAFMNMTGYSRAEVVGKNCRF-LQGPETDRAVVAQVRQAVAEKRELATELLNYRK 120 I +VN AF M G++ E+VGK PE + +A + E + R+ Sbjct: 329 ITYVNPAFCKMVGFAAEELVGKIPPMPYWAPEAMDEYQERFSNVLAGRATPQFETIFQRR 388 Query: 121 NGSTFWNALFISPVYDQHGELKYFFSSQLDISRRRDAE-------QALGQARKMEALGQL 173 +G F +F SP+ DQ G+ + S LDIS R+ AE + L + ++ ++G++ Sbjct: 389 DGERFPVLIFESPLVDQDGKQTGWMGSILDISERKRAEDLNRTQEEKLQASARLASMGEI 448 Query: 174 TGGIAHDFNNLLQVISGYLD-IINLALRSDTPDLGRVSRSADSIRKASGKAAMLTQQLLA 232 +AH+ N L IS Y +NL ++ D + + + I + +A + + + Sbjct: 449 ASTLAHELNQPLAAISSYTTGALNL-MQQGQADPKALQPALEKIHAQAQRAGHVIRSVHQ 507 Query: 233 FARKQRLEGRTINLNSLTEGFTDLVQ-RTLGGDIEVQTELASGLWNCRLDPTQMEVALLN 291 F +K+ + + + L + L++ + + +Q ++ L D +E +LN Sbjct: 508 FVKKREPARQRVQMRQLIDNVAPLIELQAQQFFVVLQLQVPRSLPPVLGDQILLEQVILN 567 Query: 292 VLINARDAMSGKGTVK--IHTSNEDLSSEDRAIQLGVKPGLYVCIAVTDNGPGIPADILP 349 + N AM+ + + + + E +ED A V ++VTD G GIP D+ Sbjct: 568 LTRNGIQAMAAVPSARRILRVAAEVTRNEDGAES--------VTVSVTDQGHGIPDDVAA 619 Query: 350 RVMDPFFTTKDEGKGTGLGLSMVYGFVKQSGGSVRIVSSAERGTTVSIYFPA 401 R+ PFF+TK E G G+GL++ ++ GG++ + GT PA Sbjct: 620 RLFSPFFSTKAE--GMGMGLNICRTTIEFHGGTLTYTDNPAGGTIFRFSLPA 669 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,662,953 Number of extensions: 68378 Number of successful extensions: 401 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 385 Number of HSP's successfully gapped: 20 Length of query: 546 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 456 Effective length of database: 1,193,720 Effective search space: 544336320 Effective search space used: 544336320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)