BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0060.p (373 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311765|ref|YP_003775857.1| adenosylmethionine-8-amino-7-ox... 619 e-179 gi|300312816|ref|YP_003776908.1| aminotransferase class III prot... 150 2e-37 gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate amino... 141 1e-34 gi|300310456|ref|YP_003774548.1| family II aminotransferase [Her... 134 1e-32 gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferas... 122 4e-29 >gi|300311765|ref|YP_003775857.1| adenosylmethionine-8-amino-7-oxononanoate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 448 Score = 619 bits (1597), Expect = e-179, Method: Compositional matrix adjust. Identities = 307/370 (82%), Positives = 327/370 (88%), Gaps = 1/370 (0%) Query: 1 LGQLEHAMLAGFTHEPVVQLSERLAALTEHALGHCFYASDGASAVEIALKMSVHAWRNAG 60 LGQLEHAMLAGFTHEPVV LSE+LAALT ALGHCFYASDGASAVEIALKMSVHAWRN G Sbjct: 78 LGQLEHAMLAGFTHEPVVALSEQLAALTGQALGHCFYASDGASAVEIALKMSVHAWRNGG 137 Query: 61 HADKNEFICIAGGYHGETVGALGVTDTPLFRSAYESLLRPAHIAASPDARQAGPGETAAD 120 DKNEFICIAGGYHGETVGALGVTDTPLFRSAYESLLRPAH+AASPDARQA PGE+A + Sbjct: 138 QPDKNEFICIAGGYHGETVGALGVTDTPLFRSAYESLLRPAHVAASPDARQAAPGESAEE 197 Query: 121 VSRRALADVERLLQERSGRIAALIVEPLVQCAAGMAMHDPQYLRGLLGLCDAYGAHLIAD 180 V+ RAL DVER+LQ+R GRIAALIVEPLVQCAAGMAMHDP Y+RGL LCD YGAHLIAD Sbjct: 198 VAARALDDVERILQQRQGRIAALIVEPLVQCAAGMAMHDPGYVRGLRSLCDRYGAHLIAD 257 Query: 181 EIAVGCGRTGRFFACEQAGVWPDLLCLSKGISGGYLPLSLVMASEAIYAAFYDDDVRRGF 240 EIAVGCGRTG FFACEQAGVWPDLLCLSKGISGGYLPLSLVM+SEAIY AFYDDDVRRGF Sbjct: 258 EIAVGCGRTGSFFACEQAGVWPDLLCLSKGISGGYLPLSLVMSSEAIYQAFYDDDVRRGF 317 Query: 241 LHSHSYTGNALACRAALATLDIFEQEQVLDRNARFAPVLAQALQPLAQDARVRHVRQRGM 300 LHSHSYTGN LACRAALATL IF ++ VL+RN FAPVLA ALQPLAQD RVRHVRQRGM Sbjct: 318 LHSHSYTGNPLACRAALATLAIFAEDAVLERNRAFAPVLAAALQPLAQDPRVRHVRQRGM 377 Query: 301 IWACDARID-DAAVAAGFSRRLADEAAQRELLVRPIGKTLYVMPPYILDAAQTQWLGERL 359 IWA DA ++ AA FSRR+A EAAQRE+LVRPIG +LY+MPPY+LD Q WLG +L Sbjct: 378 IWAYDAVLEGSAAATTAFSRRMAAEAAQREVLVRPIGASLYLMPPYLLDEEQAHWLGRQL 437 Query: 360 HAAFDAAMRA 369 HAAF A M A Sbjct: 438 HAAFAATMEA 447 >gi|300312816|ref|YP_003776908.1| aminotransferase class III protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 150 bits (379), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 116/386 (30%), Positives = 187/386 (48%), Gaps = 24/386 (6%) Query: 1 LGQLEHAMLAGFTHEPVVQLSERLAALTEHALGHCFYASDGASAVEIALKMSVHAWRNAG 60 +G+L +A + FT P +L+ LA +L H ++ S G+ AVE ALK++ + G Sbjct: 59 VGELAYAHTSFFTTAPAEELAAMLADAAPGSLNHVYFLSGGSEAVEAALKLARQYYVEVG 118 Query: 61 HADKNEFICIAGGYHGETVGALGVTDTPLFRSAYESLLRPAH-IAASPDARQAGPGETAA 119 + I YHG T+GAL + R + +L AH ++ R GE+ Sbjct: 119 QPQRRHIIARRQSYHGNTLGALAIGGNAWRREMFMPMLIEAHHVSPCYAYRNRADGESDV 178 Query: 120 DVSRRALADVE-RLLQERSGRIAALIVEPLVQCAAGMAMHDPQYLRGLLGLCDAYGAHLI 178 +R ++E ++L + ++ A + E +V AG Y R + +CD YG LI Sbjct: 179 QYVQRLADELEQKILSLGADQVIAFVAETVVGATAGAVPPVGDYFRKIRAVCDKYGVLLI 238 Query: 179 ADEIAVGCGRTGRFFACEQAGVWPDLLCLSKGISGGYLPLSLVMASEAIYAAFYDDDVRR 238 DE+ G GRTG FACE+ GV PD++ ++KG+ GY P+ ++ S+ IY D V R Sbjct: 239 LDEVMSGMGRTGYLFACEEDGVVPDIVVIAKGLGAGYQPIGAMICSDHIY-----DAVLR 293 Query: 239 G---FLHSHSYTGNALACRAALATLDIFEQEQVLDR-NARFAPVLAQALQPLAQDARVRH 294 G F H H+Y G+A AC AA+A ++E++L+ R + ++ Q A V Sbjct: 294 GSGFFQHGHTYIGHATACAAAVAVQKTIQEERLLENVRQRGEQLRSELRQAFGDQAHVGD 353 Query: 295 VRQRGMIWACD---ARIDDAAVAAGFSR--RLADEAAQRELLVRPIGKT--------LYV 341 +R RG+ + R ++ R+ EA +R LLV P+G T + + Sbjct: 354 IRGRGLFVGVELVAERSSKLPLSPDLRTHARVKAEAMKRGLLVYPMGGTIDGKNGDHILL 413 Query: 342 MPPYILDAAQTQWLGERLHAAFDAAM 367 PP+I + + +RL A A++ Sbjct: 414 APPFIASSNDISEIVQRLSDAVAASV 439 >gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 442 Score = 141 bits (355), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 106/354 (29%), Positives = 168/354 (47%), Gaps = 11/354 (3%) Query: 14 HEPVVQLSERLAALTEHALGHCFYASDGASAVEIALKMSVHAWRNAGHADKNEFICIAGG 73 H +L+ERL A T + G+ FY G+ AV+ ALK+++ R G+A + I G Sbjct: 88 HPSAFELAERLKAYTGNRFGNVFYTGSGSEAVDTALKIALAYHRAKGNATRTRLIGRERG 147 Query: 74 YHGETVGALGVTDTPLFRSAYESLLRPA--HIAASPDARQAGPGETAADVSRRALADVER 131 YHG G + V R + + L P H+ + + + + + ++ER Sbjct: 148 YHGVGFGGMSVGGIGGNRKTFSTALLPGVDHLPHTHNLEKNAFTKGEPEYGAHLADELER 207 Query: 132 LLQ-ERSGRIAALIVEPLVQCAAGMAMHDPQYLRGLLGLCDAYGAHLIADEIAVGCGRTG 190 L+ + IAA+IVEP V + G+ + YL+ L +C +G LI DE+ G GR G Sbjct: 208 LVTLHDASNIAAVIVEP-VAGSTGVLIPPKGYLKRLREICTKHGILLIFDEVITGFGRLG 266 Query: 191 RFFACEQAGVWPDLLCLSKGISGGYLPLSLVMASEAIYAAFYDDDVRRGFLHSHSYTGNA 250 FA + V PD+ +KG++ G +P+ V I+ AF + H ++Y+G+ Sbjct: 267 TPFASDYFDVEPDIFTTAKGLTNGVVPMGAVFVKPEIHDAFMNGPDGIELFHGYTYSGHP 326 Query: 251 LACRAALATLDIFEQEQVLDRNARFAPVLAQALQPLAQDARVRHVRQRGMIWACDARIDD 310 LAC A LA+L++F+ E VL+ A +A L V VR G+I A I+ Sbjct: 327 LACAAGLASLEVFQTEGVLENAQAVADYFQEAAHALRGLPHVIDVRTIGLI----AGIEL 382 Query: 311 AAVAAGFSRRLAD---EAAQRELLVRPIGKTLYVMPPYILDAAQTQWLGERLHA 361 A++ R D A +L+R G + + PP I++ Q L +L A Sbjct: 383 ASIPGRVGARAYDAFVRAFNDGILIRVTGDIIALSPPLIINKQQIDELFGKLAA 436 >gi|300310456|ref|YP_003774548.1| family II aminotransferase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 134 bits (337), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 90/340 (26%), Positives = 156/340 (45%), Gaps = 9/340 (2%) Query: 12 FTHEPVVQLSERLAALTEHALGHCFYASDGASAVEIALKMSVHAWRNAGHADKNEFICIA 71 F EP + L+++L ++ +L H ++ G+ AV+ A++ H + G K FI + Sbjct: 87 FASEPAILLAKKLVDISPASLQHVYFTLGGSDAVDAAIRYITHYHHSIGKPGKQHFISLQ 146 Query: 72 GGYHGETVGALGVTDTPLFRSAYESLLRPAHIAASPDARQAGPGETAADVSRRALADVER 131 GYHG + G+T P F + L H SP ++ A ++ A ++ Sbjct: 147 RGYHGSSSVGAGLTALPNFHYHFNLPLPTQHYLPSPYPYRSELQGDEAIIAASVQALRDK 206 Query: 132 LLQERSGRIAALIVEPLVQCAAGMAMHDPQYLRGLLGLCDAYGAHLIADEIAVGCGRTGR 191 + + + +AA EP +Q + G+ + +L+ + C+ + DE+ G GRTG Sbjct: 207 VAELGADNVAAFFCEP-IQGSGGVVVPPKGWLKAMQLACNELDILFVVDEVITGFGRTGP 265 Query: 192 FFACEQAGVWPDLLCLSKGISGGYLPLSLVMASEAIYAAFYDDDVRRGFL-HSHSYTGNA 250 FAC V PDL+ ++KG++ GY P+ VM S+ +Y D + H +Y+ + Sbjct: 266 MFACLDEDVQPDLMTMAKGLTAGYAPMGAVMMSDRVYNGIADGAPMSTVVGHGQTYSAHP 325 Query: 251 LACRAALATLDIFEQEQVLDRNARFAPVLAQALQPLAQDARVRHVRQRGMIWACDARIDD 310 ++ AL L ++E+ +LD R PV A L+ L V R RG++ A + + D Sbjct: 326 VSAAVALEVLRLYEEGGLLDNGRRLEPVFAGGLRALLAHPLVGDARSRGLLGALEL-VAD 384 Query: 311 AAVAAGFS------RRLADEAAQRELLVRPIGKTLYVMPP 344 A F+ R+ A + L+ R G + P Sbjct: 385 KQTKARFAPELKLHDRIFQAAYRHGLVFRAFGDNILGFAP 424 >gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 122 bits (307), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 113/362 (31%), Positives = 167/362 (46%), Gaps = 57/362 (15%) Query: 18 VQLSERLAALT--EHALGHCFYASDGASAVEIALKMSVHAWRNAGHADKNEFICIAGGYH 75 V+L+ER+ LT + F+ S GA AVE A+K++ H + I AGG+H Sbjct: 90 VELAERINRLTPGNYPKKTAFF-STGAEAVENAIKIA------RAHTGRPGVIAFAGGFH 142 Query: 76 GETVGALGVTDT-------------PLFRSAYESLLRPAHIAASPDARQAGPGETAADVS 122 G T+ + +T +F + Y S L H S DA +A G +D+ Sbjct: 143 GRTMMGMALTGKVAPYKLGFGPFPGDVFHAPYPSAL---HGITSEDALEAVKGLFKSDIE 199 Query: 123 RRALADVERLLQERSGRIAALIVEPLVQCAAGMAMHDPQYLRGLLGLCDAYGAHLIADEI 182 + R+AA+I+EP VQ G ++RGL LCD +G LIADE+ Sbjct: 200 AK--------------RVAAIILEP-VQGEGGFYAAPADFMRGLRALCDEHGILLIADEV 244 Query: 183 AVGCGRTGRFFACEQAGVWPDLLCLSKGISGGYLPLSLVMASEAIYAAFYDDDVRRGFLH 242 G GRTG+ FA E V PDL+ ++K ++GG +PLS V I D G L Sbjct: 245 QSGYGRTGKLFAMEHYDVLPDLMTMAKSLAGG-MPLSAVNGRAEIM-----DAPAPGGL- 297 Query: 243 SHSYTGNALACRAALATLDIFEQEQVLDRNARFAPVLAQALQPLAQDA-RVRHVRQRGMI 301 +Y GN LA +ALA LD+ E+EQ++ R R L + L+ L ++ VR G + Sbjct: 298 GGTYAGNPLAIASALAVLDVMEEEQLVTRGQRLGDKLQEHLKELRSSVPQIAEVRGVGAM 357 Query: 302 WACDARIDDAAVA---AGFSRRLADEAAQRELLVRPIGK----TLYVMPPYILDAAQTQW 354 A + D A A +++++ A LL+ G ++ P I D + Sbjct: 358 VAVE--FADPATGKPDAEYTKKVQQHALNNGLLLLTCGSYGNVIRFLFPLTIPDTVMDEA 415 Query: 355 LG 356 LG Sbjct: 416 LG 417 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.136 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,194,331 Number of extensions: 51103 Number of successful extensions: 268 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 255 Number of HSP's successfully gapped: 9 Length of query: 373 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 286 Effective length of database: 1,207,925 Effective search space: 345466550 Effective search space used: 345466550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 131 (55.1 bits)