BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0056.p (197 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311762|ref|YP_003775854.1| biotin synthase [Herbaspirillum... 277 4e-76 >gi|300311762|ref|YP_003775854.1| biotin synthase [Herbaspirillum seropedicae SmR1] Length = 347 Score = 277 bits (709), Expect = 4e-76, Method: Compositional matrix adjust. Identities = 150/247 (60%), Positives = 164/247 (66%), Gaps = 65/247 (26%) Query: 1 MSQCPSPAAAQASATLHFHRPLAAIDSS-----------AAEQLFELPFLDLLYRAQTVH 49 MS CPS A Q SA LHFHRP A +++ A LFELPFLDLLY+AQTVH Sbjct: 1 MSHCPSAPAGQTSAPLHFHRPANAANAAHSTRADDARRDAVAALFELPFLDLLYQAQTVH 60 Query: 50 RQHHRANEVQLSSLLSIKTGGCAEDCG--------------------------------- 76 RQHH AN+VQLSSLLSIKTGGCAEDCG Sbjct: 61 RQHHPANQVQLSSLLSIKTGGCAEDCGYCAQSAHHKEAGVPAGKLMAVDEVVQAARAARD 120 Query: 77 -----YC----------------SQSAXKVRALGLETCMTLGMLEQAQAEALKQAGLDYY 115 +C ++ +VRALGLETCMTLGMLEQAQAEALK+AGLDYY Sbjct: 121 QGATRFCMGAAWRNPKERDMPALTEMVSQVRALGLETCMTLGMLEQAQAEALKEAGLDYY 180 Query: 116 NHNLDTAPDFYGQVISTRSYQDRLDTLQRVQEAGLNVCCGGIIGMGETRAQRAGLIAQLA 175 NHNLDTAPDFYGQVISTRSYQDRLDTL+RV AGL+VCCGGIIGMGE+RAQRAGLIAQLA Sbjct: 181 NHNLDTAPDFYGQVISTRSYQDRLDTLERVHLAGLSVCCGGIIGMGESRAQRAGLIAQLA 240 Query: 176 SLPVPPQ 182 +LPVPPQ Sbjct: 241 ALPVPPQ 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.132 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 517,392 Number of extensions: 17997 Number of successful extensions: 61 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 60 Number of HSP's successfully gapped: 2 Length of query: 197 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 117 Effective length of database: 1,241,070 Effective search space: 145205190 Effective search space used: 145205190 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)