BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0047.p (421 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311754|ref|YP_003775846.1| Bcr/CflA family drug resistance... 719 0.0 gi|300313991|ref|YP_003778083.1| major facilitator superfamily p... 116 4e-27 gi|300312966|ref|YP_003777058.1| major facilitator superfamily p... 72 1e-13 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 65 9e-12 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 62 6e-11 >gi|300311754|ref|YP_003775846.1| Bcr/CflA family drug resistance transporter protein [Herbaspirillum seropedicae SmR1] Length = 417 Score = 719 bits (1857), Expect = 0.0, Method: Compositional matrix adjust. Identities = 360/405 (88%), Positives = 377/405 (93%) Query: 13 RHPPNHPKFLLFLICAFASAGQLAIDLYVPALPQMAIDYGTSAQAIQTSVTGYLIAYAFG 72 +HPPNHP+FLLFLICAFASAGQLAIDLYVPALP+MAIDY TSAQAIQ+SVTGYLIAYAFG Sbjct: 13 QHPPNHPRFLLFLICAFASAGQLAIDLYVPALPEMAIDYATSAQAIQSSVTGYLIAYAFG 72 Query: 73 QLAFGPLADAWGRRRVLMLGLGLFTLGCVLTLLAPNLETFVAARVLQGFGIAATNLLAKA 132 QL FGPLADA+GR+R+L LGLGLF+LGCVL+L APNLETFVAARVLQGFGIAATNLLAKA Sbjct: 73 QLVFGPLADAYGRKRILALGLGLFSLGCVLSLAAPNLETFVAARVLQGFGIAATNLLAKA 132 Query: 133 IITDSFVGQTLVHAYTYMAIAWGLAPIIAPVIGAHLQEAFGWRSCLVFLLLYSFAMWAIL 192 IITDSF GQ LVHAYTYMAIAWGLAPIIAPVIGAHLQEAFGWRSCLVFLL+YS MWA+L Sbjct: 133 IITDSFAGQALVHAYTYMAIAWGLAPIIAPVIGAHLQEAFGWRSCLVFLLVYSLGMWAVL 192 Query: 193 WRYRETLAEPVHLHPATLLKNARMVLASPVFQSCFLAQGLCYSILLVFNIVGPFMVQNTL 252 WRYRETLA+PV LHPATL +NARMVLASPVFQSCFLAQGLCYSILLVFNIVGPFMVQNTL Sbjct: 193 WRYRETLAQPVRLHPATLARNARMVLASPVFQSCFLAQGLCYSILLVFNIVGPFMVQNTL 252 Query: 253 RQPPTFFGYLALAIGMMYFLGGISNRIHHRRLPSAEQRLRIGARVMTAAAFAMLALALLV 312 QPPTFFGYLALAIGMMYFLGGISNRI HRRLP+AEQRLR+GARVMT AAF ML LAL V Sbjct: 253 HQPPTFFGYLALAIGMMYFLGGISNRITHRRLPTAEQRLRLGARVMTGAAFVMLLLALSV 312 Query: 313 GLTVWTLITPVLVMAFCAGAMYPTLMAKGNSIFPHIAGLTSAILGFALLLVSSGMMGLAG 372 GLTVWTL PVLVMAFCAGAMYPTLMAKGNSIFPHIAGLTSAILGFALLLVS+ MMGLAG Sbjct: 313 GLTVWTLCAPVLVMAFCAGAMYPTLMAKGNSIFPHIAGLTSAILGFALLLVSAAMMGLAG 372 Query: 373 FVSIHSLTPLAVFFCIVGLTVIAMVGKLLQHLQVPAAAATLQVRP 417 FVSIHSLTPLA FFC+VGLTVI MVGKLL HLQVPA A RP Sbjct: 373 FVSIHSLTPLAGFFCVVGLTVIVMVGKLLHHLQVPAPLAQPVARP 417 >gi|300313991|ref|YP_003778083.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 406 Score = 116 bits (290), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 79/256 (30%), Positives = 136/256 (53%), Gaps = 13/256 (5%) Query: 23 LFLICAFASAGQLAIDLYVPALPQMAIDYGTSAQAIQTSVTGYLIAYAFGQLAFGPLADA 82 L ++ + G LAID+Y+P+ +A D G +Q ++ +LI A GQ+ +GPL+D Sbjct: 17 LAVLAGLTAMGALAIDMYLPSFTAIAQDLGVGTNQVQLTLATFLIGVAVGQMFYGPLSDR 76 Query: 83 WGRRRVLMLGLGLFTLGCVLTLLAPNLETFVAARVLQGFGIAATNLLAKAIITDSFVGQT 142 +GR+ L G+ L+ + +L + A N+ET + R+ QG G +A ++ +A++ D + Sbjct: 77 FGRKPPLFFGIALYFVCSLLCIFAQNIETLIVLRLFQGLGGSAGMVIPRAMVRDRMGAEG 136 Query: 143 LVHAYTYMAIAWGLAPIIAPVIGAHLQEAFGWRSCLVFLLLYSFAMWAILWRYR---ETL 199 A++ + + +GLAPI+AP IG + WR +F++L F + IL + ET+ Sbjct: 137 SAKAFSMLMLVFGLAPILAPFIGGLMLVFASWRG--IFVVLTLFGLLCILGTRKYLTETV 194 Query: 200 ----AEPVHLHPATLLKNARMVLASPVFQSCFLAQGLCYSILLVFNIVGPFMVQNTLRQP 255 AEP+HL L+ +L F S L GL + + + GPF+V Sbjct: 195 DVSRAEPLHL--GRTLRQYWGLLRHRQFMSYVLCGGLVQAGMFAYIAGGPFVVIELHGVK 252 Query: 256 PTFFGYL--ALAIGMM 269 P +FG++ + AIG++ Sbjct: 253 PQYFGFVFASNAIGLI 268 >gi|300312966|ref|YP_003777058.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 544 Score = 71.6 bits (174), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 61/185 (32%), Positives = 85/185 (45%), Gaps = 19/185 (10%) Query: 44 LPQMAIDYGTSAQAIQTSVTGYLIAYAFGQLAFGPLADAWGRRRVLMLGLGLFTLGCVLT 103 LP +A D G S I+ V+ Y++ +A L G AD +GRRRVL+ G+ LF L + Sbjct: 59 LPTVARDLGASFSQIEWVVSSYVLCFAALLLPAGATADRYGRRRVLLGGIALFALTSLAC 118 Query: 104 LLAPNLETFVAARVLQGFGIAATNLLAKAIITDSFVGQTLVHAYTYMAIAW-------GL 156 LAP+ + AR LQG G A A A+I +F H AW GL Sbjct: 119 ALAPSAQALYGARALQGVGAAFLLAPALAVIGHAF------HDEDQRERAWALWGGIMGL 172 Query: 157 APIIAPVIGAHLQEAFGWRSCLVF----LLLYSFAMWAILWRYRETLAEPVHLHPATLLK 212 ++AP+IG + GWR LL + A+W + R L P L P +L Sbjct: 173 TMVLAPLIGGVVNTLLGWRWAFAVNLPACLLLAVAVWRHVPESRNPLPRP--LDPLGILS 230 Query: 213 NARMV 217 ++ V Sbjct: 231 SSSAV 235 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 65.5 bits (158), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 48/168 (28%), Positives = 80/168 (47%), Gaps = 3/168 (1%) Query: 43 ALPQMAIDYGTSAQAIQTSVTGYLIAYAFGQLAFGPLADAWGRRRVLMLGLGLFTLGCVL 102 +LP +A D ++ ++T Y+ A G +AD G R+V + +F G +L Sbjct: 9 SLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMIVFMTGSIL 68 Query: 103 TLLAPNLETFVAARVLQGFGIAATNLLAKAIITDSFVGQTLVHAYTYMAIAWGLAPIIAP 162 ++ +L TFVAAR LQG G A + + II S LV A +Y+ + L P++ P Sbjct: 69 CAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPALLGPVVGP 128 Query: 163 VIGAHLQEAFGWRSCLVFLLLYSFAMWAILWRYRETLAEPVHLHPATL 210 ++G + F WR + + + F + R+ + E +PA L Sbjct: 129 LLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKED---NPAPL 173 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 62.4 bits (150), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 47/161 (29%), Positives = 77/161 (47%) Query: 14 HPPNHPKFLLFLICAFASAGQLAIDLYVPALPQMAIDYGTSAQAIQTSVTGYLIAYAFGQ 73 + P + LL+++ + L + ALP MA G + V Y + A Sbjct: 46 NDPVTQRALLWIVASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLT 105 Query: 74 LAFGPLADAWGRRRVLMLGLGLFTLGCVLTLLAPNLETFVAARVLQGFGIAATNLLAKAI 133 A G LAD +G RR+ + LF+LG +L +A +L +AARV+QG G + + + Sbjct: 106 PASGWLADRFGTRRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLA 165 Query: 134 ITDSFVGQTLVHAYTYMAIAWGLAPIIAPVIGAHLQEAFGW 174 + + G + A ++IA L P++ PV+G L E+ W Sbjct: 166 VLRAVPGAQYIAALASISIAGQLGPVVGPVLGGWLVESLSW 206 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.141 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,231,208 Number of extensions: 48841 Number of successful extensions: 325 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 325 Number of HSP's successfully gapped: 16 Length of query: 421 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 333 Effective length of database: 1,203,190 Effective search space: 400662270 Effective search space used: 400662270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 132 (55.5 bits)