BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0035.p (200 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311703|ref|YP_003775795.1| ABC-type export system outer me... 140 7e-35 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 113 1e-26 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 107 7e-25 gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspiri... 106 1e-24 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 105 3e-24 >gi|300311703|ref|YP_003775795.1| ABC-type export system outer membrane channel protein [Herbaspirillum seropedicae SmR1] Length = 489 Score = 140 bits (353), Expect = 7e-35, Method: Compositional matrix adjust. Identities = 78/195 (40%), Positives = 122/195 (62%) Query: 1 LPAELPLSLPSTLVQQRPDVRAAQAQLQAASAQVGVAIANRLPSFNITGTIGSSAQEFSK 60 LP+++P+SLPS LV++RPD+ AA+A++ AASA +G++ A + PS +T T G +A+ S+ Sbjct: 287 LPSDIPVSLPSALVRRRPDILAAEARMHAASAAIGMSSAAQYPSLELTATYGVAARNLSQ 346 Query: 61 LFTSGPGFWTLGLDLATPLFHGGSLQHQQRAAEAGLDQAGAQYRSVVLGAFQNVADSLHA 120 L W+ G L+TPLF+GG L+ Q+ AA + + ++Y+ VL AF+ VAD+L A Sbjct: 347 LTGPATSLWSFGPSLSTPLFNGGRLRAQRDAAVNAFEASQSEYQQTVLTAFEQVADALRA 406 Query: 121 IAADADGLAAATRAESAAQRSFDAAQAQWKAGIAGYPQVLLAQQNLLTASQSRLQAQAAR 180 + AD ++A + A A + +A + AG++ Y +LL + +A+ + LQA R Sbjct: 407 LQADGHIVSAQSAAARYAYDTLLLQRASYSAGLSSYADMLLYEVQYRSAAIASLQATVQR 466 Query: 181 LADTVALFQALGGGW 195 DTVAL A GGGW Sbjct: 467 YQDTVALLVASGGGW 481 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 113 bits (282), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 66/198 (33%), Positives = 112/198 (56%), Gaps = 1/198 (0%) Query: 1 LPAELPLSLPSTLVQQRPDVRAAQAQLQAASAQVGVAIANRLPSFNITGTIGSSAQEFSK 60 LPA P LPS ++ +RPDV AA+ L++A+A +G A A PS ++T G++++ Sbjct: 291 LPAT-PAGLPSQVLLRRPDVLAAEHTLKSANADIGAARAAFFPSISLTANGGTASRTLGG 349 Query: 61 LFTSGPGFWTLGLDLATPLFHGGSLQHQQRAAEAGLDQAGAQYRSVVLGAFQNVADSLHA 120 LF +G G WT + P+F+ GSL+ +A+ D A Y + AF+ VAD+L Sbjct: 350 LFKAGSGSWTFSPSINLPIFNAGSLRASLDSAKITRDIDVANYEKAIQTAFREVADALAV 409 Query: 121 IAADADGLAAATRAESAAQRSFDAAQAQWKAGIAGYPQVLLAQQNLLTASQSRLQAQAAR 180 A L A +A +A++++F + A+++ G+ Y L+AQ+ L +A Q+ + + Sbjct: 410 RATLDQRLDAQGKATAASEKAFRLSDARYRNGVDSYLTTLVAQRTLYSAQQTLISLRLTE 469 Query: 181 LADTVALFQALGGGWWHQ 198 ++ + L++ LGGGW Q Sbjct: 470 QSNRITLYKVLGGGWSEQ 487 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 107 bits (267), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 67/191 (35%), Positives = 103/191 (53%) Query: 4 ELPLSLPSTLVQQRPDVRAAQAQLQAASAQVGVAIANRLPSFNITGTIGSSAQEFSKLFT 63 ++P LPS L+ RPD+R+A+ L AA+A +G A A P ++TG GS++ LF Sbjct: 281 DIPAGLPSDLLTNRPDIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFE 340 Query: 64 SGPGFWTLGLDLATPLFHGGSLQHQQRAAEAGLDQAGAQYRSVVLGAFQNVADSLHAIAA 123 SG G W+ G L+ P+F G AE ++A Y + AF+ VAD+L A + Sbjct: 341 SGSGAWSFGPSLSLPIFDFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGS 400 Query: 124 DADGLAAATRAESAAQRSFDAAQAQWKAGIAGYPQVLLAQQNLLTASQSRLQAQAARLAD 183 + + A +A Q ++K GIA L AQ++L +A Q+ +QA+ RL + Sbjct: 401 LDEQVKAQEAFLAAQQERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNN 460 Query: 184 TVALFQALGGG 194 + L++ALGGG Sbjct: 461 AIDLYKALGGG 471 >gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 507 Score = 106 bits (264), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 71/196 (36%), Positives = 104/196 (53%) Query: 5 LPLSLPSTLVQQRPDVRAAQAQLQAASAQVGVAIANRLPSFNITGTIGSSAQEFSKLFTS 64 +P +PS L+++RPDV AA+ ++ +A+A++GVA A PS ++ + G + F+ FT Sbjct: 288 VPQGVPSMLLERRPDVAAAERRVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTL 347 Query: 65 GPGFWTLGLDLATPLFHGGSLQHQQRAAEAGLDQAGAQYRSVVLGAFQNVADSLHAIAAD 124 W+LG LA +F GG+ A A D A Y+ VL AFQ V D+L A+ Sbjct: 348 PGRVWSLGPQLAQTIFDGGARAAATDQAIATYDGTVATYKQTVLTAFQEVEDNLAALRIL 407 Query: 125 ADGLAAATRAESAAQRSFDAAQAQWKAGIAGYPQVLLAQQNLLTASQSRLQAQAARLADT 184 A A AA+++ Q+KAG Y V+ AQ L+A S L R+A + Sbjct: 408 EQEAAVQGEALQAARQAVTLVNNQYKAGTVSYVNVITAQATALSAELSSLNILNRRMAAS 467 Query: 185 VALFQALGGGWWHQDL 200 V L +ALGGGW + L Sbjct: 468 VLLAKALGGGWDERSL 483 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 105 bits (261), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 67/194 (34%), Positives = 110/194 (56%) Query: 2 PAELPLSLPSTLVQQRPDVRAAQAQLQAASAQVGVAIANRLPSFNITGTIGSSAQEFSKL 61 P +P+ +PS ++++RPD+ AA+ + AA+AQ+GVA A PSF + + G ++ ++ L Sbjct: 276 PPAIPVGVPSDVLERRPDIAAAERAMAAANAQIGVASAAYYPSFMLQPSYGVDSRNWATL 335 Query: 62 FTSGPGFWTLGLDLATPLFHGGSLQHQQRAAEAGLDQAGAQYRSVVLGAFQNVADSLHAI 121 F + W+LG+ + LF GG L+ ++AG + A YR VL A Q V D + + Sbjct: 336 FNAPSLLWSLGVSASQSLFDGGRLRAGVDFSQAGYEATVASYRRTVLTAMQEVEDGITGL 395 Query: 122 AADADGLAAATRAESAAQRSFDAAQAQWKAGIAGYPQVLLAQQNLLTASQSRLQAQAARL 181 AA A + A ++A+R + A ++++ G Y V+ AQQ+LL + + Q RL Sbjct: 396 AALERAYAQSQLAIASARRVLEIASSRYEGGATPYLDVITAQQSLLNSERQAAQLMGQRL 455 Query: 182 ADTVALFQALGGGW 195 +V L +ALGG W Sbjct: 456 LVSVFLIKALGGDW 469 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.128 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 520,281 Number of extensions: 16741 Number of successful extensions: 141 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 127 Number of HSP's successfully gapped: 19 Length of query: 200 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 119 Effective length of database: 1,236,335 Effective search space: 147123865 Effective search space used: 147123865 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)