BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0033.p (169 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance ... 110 7e-26 gi|300313244|ref|YP_003777336.1| multidrug efflux system transme... 72 3e-14 gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 69 2e-13 gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump pr... 64 6e-12 gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump pr... 62 3e-11 >gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1073 Score = 110 bits (274), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 61/123 (49%), Positives = 88/123 (71%), Gaps = 1/123 (0%) Query: 1 MALALMFVLLFAEFKNLRQPALILLCVPLATLGGLVALHLRGMTLNVSSAVGFIALFGVA 60 +AL ++FVLLFA F N++ L+ +P A GG++AL +RG+ +++S+AVGFIAL GVA Sbjct: 888 VALLMVFVLLFAMFSNVKDGLLVFTGIPFALTGGILALAMRGIPMSISAAVGFIALSGVA 947 Query: 61 VLNAIIMMSNLNRWVARSGLPLREAVISAALERMRPVLMTATVAALGLVPAAIAHGLGSD 120 VLN ++M+S + R + G L A+ A+ R+RPVLMTA VA+LG VP A+A G G++ Sbjct: 948 VLNGLVMISFI-RSLREEGRTLDAAIHEGAMTRLRPVLMTALVASLGFVPMALATGTGAE 1006 Query: 121 VQR 123 VQR Sbjct: 1007 VQR 1009 >gi|300313244|ref|YP_003777336.1| multidrug efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1033 Score = 71.6 bits (174), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 46/125 (36%), Positives = 77/125 (61%), Gaps = 3/125 (2%) Query: 1 MALALM--FVLLFAEFKNLRQPALILLCVPLATLGGLVALHLRGMTLNVSSAVGFIALFG 58 MAL+++ F++L A +++ P ++L VPL +G + A+ LR M +V VG I + G Sbjct: 870 MALSMLVVFLVLAALYESWSIPLSVMLVVPLGLVGAVAAVILRDMPNDVFFKVGMITVIG 929 Query: 59 VAVLNAIIMMSNLNRWVARSGLPLREAVISAALERMRPVLMTATVAALGLVPAAIAHGLG 118 ++ NAI+++ R + G+ L EA I A++ R+RP+LMT+ ALG+VP +A G Sbjct: 930 LSAKNAILIV-EFARQLRSEGMGLVEAAIKASVLRLRPILMTSLAFALGVVPLMLASGAS 988 Query: 119 SDVQR 123 ++ QR Sbjct: 989 AETQR 993 Score = 57.4 bits (137), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 35/115 (30%), Positives = 62/115 (53%) Query: 2 ALALMFVLLFAEFKNLRQPALILLCVPLATLGGLVALHLRGMTLNVSSAVGFIALFGVAV 61 A+ L+F++++ +N+R + + P+A LG L + L G ++NV + G + G+ V Sbjct: 347 AMVLVFLVMYLFLQNVRYTVIPAIVAPIALLGTLAVMQLAGYSVNVLTMFGMVLAIGIIV 406 Query: 62 LNAIIMMSNLNRWVARSGLPLREAVISAALERMRPVLMTATVAALGLVPAAIAHG 116 +AI+++ N+ R +A GL REA I A E ++ V +P A+A G Sbjct: 407 DDAIVVVENVERIMAHEGLGPREATIKAMGEITGAIIGITLVLTAVFIPMALAGG 461 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 68.9 bits (167), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 45/123 (36%), Positives = 72/123 (58%), Gaps = 1/123 (0%) Query: 1 MALALMFVLLFAEFKNLRQPALILLCVPLATLGGLVALHLRGMTLNVSSAVGFIALFGVA 60 ++L +F++L A +++ P ++L VPL LG L+ + LR M +V VG IA+ G++ Sbjct: 874 LSLIAVFLVLAALYESTSIPLAVMLVVPLGILGALLGVTLRAMPNDVYFKVGMIAVVGLS 933 Query: 61 VLNAIIMMSNLNRWVARSGLPLREAVISAALERMRPVLMTATVAALGLVPAAIAHGLGSD 120 NAI+++ + + G L EA + A R RP++MT+ LG++P AIA G GS Sbjct: 934 AKNAILII-EFAKDLQAQGKGLIEATLEAVHLRFRPIIMTSLAFILGVLPLAIASGAGSA 992 Query: 121 VQR 123 QR Sbjct: 993 SQR 995 >gi|300310853|ref|YP_003774945.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1078 Score = 63.9 bits (154), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 39/122 (31%), Positives = 69/122 (56%) Query: 2 ALALMFVLLFAEFKNLRQPALILLCVPLATLGGLVALHLRGMTLNVSSAVGFIALFGVAV 61 AL ++++L +++L P I+ +P A +G L+AL + G ++ + +G I L G+ Sbjct: 907 ALLAVYIVLGILYESLIHPITIISTLPSAGVGALLALMVTGTDFSLIALIGVILLIGIVK 966 Query: 62 LNAIIMMSNLNRWVARSGLPLREAVISAALERMRPVLMTATVAALGLVPAAIAHGLGSDV 121 NAI+M+ G+ R+A+ A R RP++MT A LG +P AI HG G+++ Sbjct: 967 KNAIMMVDFAIDAERNRGMSPRDAIFEACKLRFRPIMMTTMAALLGAIPLAIGHGDGAEL 1026 Query: 122 QR 123 ++ Sbjct: 1027 RQ 1028 >gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 61.6 bits (148), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 40/124 (32%), Positives = 71/124 (57%), Gaps = 3/124 (2%) Query: 2 ALALMFVLLF--AEFKNLRQPALILLCVPLATLGGLVALHLRGMTLNVSSAVGFIALFGV 59 A++++FV L A +++ P ++L VPL +G ++ + GM+ +V VG + G+ Sbjct: 876 AISILFVFLCLAALYESWSVPFAVILAVPLGIIGTVLLTGIFGMSNDVYFQVGLLTTVGL 935 Query: 60 AVLNAIIMMSNLNRWVARSGLPLREAVISAALERMRPVLMTATVAALGLVPAAIAHGLGS 119 + NAI+++ + + SG L EA + A R+RP+LMT+ G++P A+A G GS Sbjct: 936 SAKNAILIV-EFAKQLHESGKSLWEATLEAVQLRLRPILMTSLAFMFGVLPLAVASGAGS 994 Query: 120 DVQR 123 +R Sbjct: 995 ASRR 998 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.139 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 571,616 Number of extensions: 26177 Number of successful extensions: 189 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 181 Number of HSP's successfully gapped: 13 Length of query: 169 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 90 Effective length of database: 1,245,805 Effective search space: 112122450 Effective search space used: 112122450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 127 (53.5 bits)