BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0032.p (369 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance ... 203 1e-53 gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 65 9e-12 gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump tr... 56 4e-09 >gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1073 Score = 203 bits (517), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 117/385 (30%), Positives = 205/385 (53%), Gaps = 18/385 (4%) Query: 1 VLAWLEPRYRQVLGRLVGKSRFVLAATALTLAAIGVLGATIGRDFLPYLDEGSIWLQVQL 60 ++ W + Y +L ++ VL + GV+ + +G +F+P L+EG + +Q Sbjct: 518 LMLWAKRLYEPMLKTIMENKPVVLTFAGALILVCGVVASRLGSEFVPSLNEGDMAIQALR 577 Query: 61 PPGVSLDKASELADRL-RAATREFPEVEHIVTQVGRNDDGTDPFTPSHIEAAVTLHPYSE 119 PG SL ++ E+ ++ + ++FPEVE + + G + +DP P+ + + L P + Sbjct: 578 IPGTSLSQSLEMQKQIEKTLKQKFPEVERVFARTGTAEVASDPMPPNISDGYIMLKPQEQ 637 Query: 120 WAS-GWTKQELIAHMAERYKKLAGIDVGFTQPMIDGVLDKLSGSHSDLAVKIYGNDFGEM 178 W +++EL+ + + K+ G F+QP+ + +SG SD+AVK++G+D + Sbjct: 638 WPEPKKSREELLEAIQDEVAKIPGNSYEFSQPIQLRFNELISGVRSDVAVKVFGDDMKVL 697 Query: 179 RVLATAVQGVLQDVPGAADVVIDQEPRLPQVRVNVDRAAAARLGINVADIMALIQTGIGG 238 A+ + VL+ +PGA +V I+Q LP + V +DR AR G+NV+DI + T IGG Sbjct: 698 NDTASKISAVLEKIPGATEVKIEQTTGLPMLTVEIDREKTARYGLNVSDIQDTVATAIGG 757 Query: 239 SPVTQVFIEERSYNVAAVFNGRARSTPEALGNLTLV------AANGAHIALSQVAHISME 292 +F +R + + +AR+ EA+ L + A +++ L +VA +++ Sbjct: 758 KEAGTMFQGDRRFGIVVRLPDQARTDLEAIRRLPIALPPQEGATRVSYVPLGEVASLTIA 817 Query: 293 DGETTITREMNRRHLTVRLNLRGRDLASFLDDAKARIAQQVQYDHGRYQVAWGGQFENQQ 352 G I+RE +R + V N+RGRDL F+ +A+ ++ V+ G Y WGGQFEN Q Sbjct: 818 PGPNQISREDGKRRIVVSANVRGRDLGGFVAEAERQLQSAVKVPAG-YWTTWGGQFENLQ 876 Query: 353 ---------LPMALALMFVLLFAXW 368 +P+AL ++FVLLFA + Sbjct: 877 SATQRLQIVIPVALLMVFVLLFAMF 901 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 65.1 bits (157), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 65/296 (21%), Positives = 123/296 (41%), Gaps = 12/296 (4%) Query: 9 YRQVLGRLVGKSRFVLAATALTLAAIGVLGATIGRDFLPYLDEGSIWLQVQLPPGVSLDK 68 Y+ ++G ++ K+ + L +A +G+L + FLP D+G I VQLPPG S + Sbjct: 526 YQGIIGGMLNKAGRYMIVYVLIVACVGLLYVRLPASFLPNEDQGYIITNVQLPPGASTSR 585 Query: 69 ASELADRLRAATREFPEVEHIVTQVGRNDDGTDPFTPSHIEAAVTLHPYSEWASGWTKQE 128 + + ++ + P VEH + +G + F+ + AA+ P +W+ Q Sbjct: 586 TTAVVKQIEEYYLKDPAVEHAIAVLGYS------FSGNGQNAALVFTPLKDWSQRDKSQS 639 Query: 129 LIAHMAERYKKLAGIDVGFTQPMIDGVLDKLSGSHSDLAVKIYGNDFGEMRVLATAVQGV 188 A + + + I P+ + +L G+ + ++ L A + Sbjct: 640 ADAIAGKAFGAFSQIKDAIVFPLNPPPIPEL-GNATGFTFRLQDRAGLGHTALVQARNQI 698 Query: 189 LQDVPGAADVVIDQEPR----LPQVRVNVDRAAAARLGINVADIMALIQTGIGGSPVTQV 244 L + + V+ P PQ+++++DR A LG+ + I +++ T +G S V Sbjct: 699 L-GMASQSKVLAGVRPEGLEDAPQLQLDIDRDKANALGVPFSTINSVLSTALGSSYVNDF 757 Query: 245 FIEERSYNVAAVFNGRARSTPEALGNLTLVAANGAHIALSQVAHISMEDGETTITR 300 R V + + R PE L NL ++G + S +G + R Sbjct: 758 PNLGRQQRVIVQADAKRRLQPEDLTNLYARNSSGTMVPFSAFVTAKWINGPVQLIR 813 >gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 56.2 bits (134), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 73/323 (22%), Positives = 130/323 (40%), Gaps = 11/323 (3%) Query: 8 RYRQVLGRLVGKSRFVLAATALTLAAIGVLGATIGRDFLPYLDEGSIWLQVQLPPGVSLD 67 RY +L ++ + + L A TL +L + + F P D G I + +S Sbjct: 515 RYGVMLQWVLARQKLTLLVAAATLGLTVLLYVFVPKGFFPLQDTGVIQGISEATQSISFS 574 Query: 68 KASELADRLRAATREFPEVEHIVTQVGRNDDGTDPFTPSHIEAAVTLHPYS--EWASGWT 125 E +L + P VE + + +G DGT+ T + + L P+ E ++ Sbjct: 575 AMGEKQQQLAKVVLQDPAVESLTSFIGV--DGTNA-TLNSGRMLINLKPHDVREVSATDV 631 Query: 126 KQELIAHMAERYKKLAGIDVGFTQPMIDGVLDKLSGSHSDLAVKIYGNDFGEMRVLATAV 185 + L +A ++ GI + + QP+ D ++ S S + + D + + Sbjct: 632 IRRLQGKLA---AEVPGITL-YMQPVQDLTIED-SVSRTQYQFTLEDADPAVLSTWVPKL 686 Query: 186 QGVLQDVPGAADVVIDQEPRLPQVRVNVDRAAAARLGINVADIMALIQTGIGGSPVTQVF 245 LQ ADV + + + Q + +DR AA+RLGI A I + G ++ ++ Sbjct: 687 IARLQQQSELADVATNLQDQGLQAYIEIDRNAASRLGITTAAIDNALYNAFGQRLISTIY 746 Query: 246 IEERSYNVAAVFNGRARSTPEALGNLTLVAANGAHIALSQVAHISMEDGETTITREMNRR 305 + Y V + P AL ++ LV + G + LS +A ++ + Sbjct: 747 TQSNQYRVVMEVKPEFQHGPAALDSIYLVTSGGGKVPLSSIATVTERTAPLVVNHIGQFP 806 Query: 306 HLTVRLNL-RGRDLASFLDDAKA 327 TV NL +G L + KA Sbjct: 807 AATVSFNLAKGASLGEAVKAIKA 829 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,083,584 Number of extensions: 42614 Number of successful extensions: 181 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 178 Number of HSP's successfully gapped: 6 Length of query: 369 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 282 Effective length of database: 1,207,925 Effective search space: 340634850 Effective search space used: 340634850 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)