BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0031.p (517 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance ... 314 1e-86 gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump tr... 130 2e-31 gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 125 7e-30 gi|300313244|ref|YP_003777336.1| multidrug efflux system transme... 112 7e-26 gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump pr... 110 2e-25 >gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1073 Score = 314 bits (804), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 183/475 (38%), Positives = 276/475 (58%), Gaps = 35/475 (7%) Query: 1 MIERLVNLCFQRRGIVQLVFLLAALYGWYCWNQLPLEAYPDIADVTSQVVTQVNGLAAEE 60 M ER++ + R +V L L YG Y + +LP++A PDI +V Q+ T G + E Sbjct: 1 MFERIIRFAIEHRWLVLLAVLGMGAYGVYSYQKLPIDAVPDITNVQVQINTSAPGYSPLE 60 Query: 61 VEQQITIPLERQIMGTPGMHVMRSESTFGLSLITVVFNDGAEDYWSRQRLQERI--AAVS 118 EQ+I+ P+E + G P + +RS S +GLS +TV+F DG + Y++RQ + ERI A Sbjct: 61 AEQRISYPIETLMSGLPNLEQVRSLSRYGLSQVTVIFKDGTDIYFARQLVNERIQLARSQ 120 Query: 119 LPYGAQPGLDALTSPIGEIYRYTLQSKTRDLR----------ELSELQFWKVIPRLKQVS 168 LP G PG+ +++ +GEIY +T++S+ D R +L E+Q W + P+L+ V Sbjct: 121 LPPGIDPGMGPISTGLGEIYLWTVESRD-DARKPDGTPYTATDLREIQDWVIKPQLRLVP 179 Query: 169 GVVDVSNFGGLTTQFMLQLDPAKLVKYNTSLSQISQAISANNANAG-GSIMKRGEQGLVI 227 GV +++ GG ++++ KL Y ++ I +A+ NN+N G G I +RGEQ L I Sbjct: 180 GVTEINTIGGFAKEYLVAPSAEKLASYGMTMDTIIKALEKNNSNVGAGYIERRGEQYL-I 238 Query: 228 RGVGLIRSLDDLGNIVVSQKNGVPVLVKDLGQVVLGNQERHGVLGIDGQSDAIEGITLLL 287 R G + S+DD+GNIV++ GVP+ ++D+ +V +G + R G +G+ + + G +L Sbjct: 239 RAPGQVASIDDIGNIVLANVKGVPIRIRDVAEVEIGRELRTGAATENGR-EVVLGTVFML 297 Query: 288 KNENPSRVMEGVHAAVQDLNEHILPKDVKIVPYIDRSKLVDATVHTVGKTLVEGMLLVSL 347 EN +V + V ++++N LPK V+ V DR+ LVD + TV K LVEG LLV Sbjct: 298 IGENSRKVSQAVARKMEEINRS-LPKGVEAVTVYDRTVLVDKAIATVKKNLVEGALLVVA 356 Query: 348 VLLLFLGSARAAAIVAMTIPLSLLTAFILMHHFKIPANLLSLGAIDFGILVDGAIVLVES 407 VL +FLG+ RAA I AM IPL++L F M K+ ANLLSLGA+DFGI++DGA+V+VE+ Sbjct: 357 VLFVFLGNIRAAIITAMVIPLAMLFTFTGMVSNKVSANLLSLGALDFGIIIDGAVVIVEN 416 Query: 408 VLR-------------LREERPQGEITGADIQQLMRQLRPIFFGMLVIIIAYLPL 449 +R R ER A RP+ FG L+I+I YLP+ Sbjct: 417 CVRRLAHAQAHHGRTLTRSERFHEVFAAAK-----EARRPLIFGQLIIMIVYLPM 466 >gi|300310852|ref|YP_003774944.1| cation/multidrug efflux pump transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 130 bits (328), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 137/490 (27%), Positives = 228/490 (46%), Gaps = 35/490 (7%) Query: 18 LVFLLAALYGWYCWNQLPLEAYPDIADVTSQVVTQVNGLAAEEVEQQITIPLERQIMGTP 77 L+ L L G + LPL A P++ T QVVT G + + + +T PLERQ P Sbjct: 16 LLMLAILLVGIVAYRLLPLSALPEVDYPTIQVVTLYPGASPDVMTSSVTAPLERQFGQMP 75 Query: 78 GMHVMRSESTFGLSLITVVFNDGAEDYWSRQRLQERIAAVS--LPYG-AQPGLDALTSPI 134 G+ M S S+ G S+IT+ FN + Q +Q I A S LP P + + +P Sbjct: 76 GLKQMSSTSSGGASVITLQFNLDLSLDIAEQEVQAAINAGSNLLPSDLPMPPVYSKVNPA 135 Query: 135 GE-IYRYTLQSKTRDLRELSELQFWKVIPRLKQVSGVVDVSNFGGLTTQFMLQLDPAKLV 193 I + S T L ++ +L ++ ++ Q+SGV VS GG +QL+P + Sbjct: 136 DTPIITLAVTSNTMPLPKVQDLIDTRIAQKISQLSGVGLVSLSGGQRPAVRMQLNPRAVA 195 Query: 194 KYNTSLSQISQAISANNANAGGSIMKRGEQGLVIRGVGLIRSLDDLGNIVVSQKNGVPVL 253 +L + AI N N + I +RS D+ N++++ KNG P+ Sbjct: 196 ALGLNLDDVRTAIGNANVNQAKGSFDGPTRASTIDANDQLRSADEYRNLIIAYKNGAPIR 255 Query: 254 VKDLGQVVLGNQERHGVLGIDGQSDAIEGITLLLKNENPSRVMEGVHAAVQDLNEHILPK 313 V D+ VV G + + + ++ + + ++ + + V+ AV D + ILP+ Sbjct: 256 VSDVADVVDGAEN----VRLGAWANTQPAVIVNIQRQPGANVI-----AVVDSIKKILPQ 306 Query: 314 ---------DVKIVPYIDRSKLVDATVHTVGKTLVEGMLLVSLVLLLFLGSARAAAIVAM 364 DV I+ DR+ + A+V V L+ + LV +V+ +FL + A I ++ Sbjct: 307 LQASLPGAIDVHIL--TDRTTTIRASVDDVKFELLLSVALVVMVIFVFLRNVPATIIPSV 364 Query: 365 TIPLSLLTAFILMHHFKIPANLLSLGA--IDFGILVDGAIVLVESVLRLREE--RP-QGE 419 +PLSL+ F +M+ N L+L A I G +VD AIV++E++ R EE +P Q Sbjct: 365 AVPLSLVGTFAIMYLAGFSINNLTLMALTIATGFVVDDAIVMIENISRYIEEGMKPMQAA 424 Query: 420 ITGADIQQLMRQLRPIFFGMLVIIIAYLPLXCAADLLRHAGHHHCLPAPVR-LPAHRVQA 478 + GA+ Q+ + + F ++ ++I PL D++ H + V L + V Sbjct: 425 LKGAE--QIGFTIISLTFSLIAVLI---PLLFMGDVVGRLFHEFAVTLAVSILISAVVSL 479 Query: 479 VRADGLCGRL 488 +C RL Sbjct: 480 TLTPMMCARL 489 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 125 bits (314), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 128/448 (28%), Positives = 214/448 (47%), Gaps = 35/448 (7%) Query: 25 LYGWYCWNQLPLEAYPDIADVTSQVVTQVNGLAAEEVEQQITIPLERQIMGTPGMHVMRS 84 L G LP+ YP+IA T V G A+ ++ +T +E+++ G G+ + S Sbjct: 21 LAGALAITGLPVSQYPNIAPPTIVVTATYPGATAQTLDDSVTSLIEQEMNGADGLQYIES 80 Query: 85 ES-TFGLSLITVVFND------GAEDYWSR-QRLQERIAAVSLPYGAQPGLDALTSPIGE 136 +S G IT+ F+ A D +R +R++ R+ A G Q +T Sbjct: 81 QSQANGQVQITITFSPETNPDLAAVDVQNRLKRVEARLPAAVTQQGVQ-----VTKSRSN 135 Query: 137 IYRYTLQSKTR---DLRELSELQFWKVIPRLKQVSGVVDVSNFGGLTTQFM-LQLDPAKL 192 + S T D L + V+ +K+V GV FG T + M + +DP KL Sbjct: 136 FLLFIGLSSTDGKLDPVALGDYLSRNVLNEIKRVPGVGQAQLFG--TERAMRIWIDPNKL 193 Query: 193 VKYNTSLSQISQAISANNANAGGSIM----KRGEQGLVIRGV--GLIRSLDDLGNIVV-S 245 + N + + ++ AI A NA G + G Q + V G + S + GNI++ + Sbjct: 194 IGLNLTPADVTAAIQAQNALVAGGTLGALPSPGTQQIAATVVVNGQLTSPEQFGNIILRA 253 Query: 246 QKNGVPVLVKDLGQVVLGNQERHGVLGIDGQSDAIEGITLLLKNENPSRVMEGVHAAVQD 305 NG V ++D+ +V +G Q +DG+ + G+ L N VH +++ Sbjct: 254 NANGATVRLRDVARVEVGGQSYDTSGRLDGKPSSFIGVQLS-PTANALGTATAVHKKMEE 312 Query: 306 LNEHILPKDVK-IVPYIDRSKLVDATVHTVGKTLVEGMLLVSLVLLLFLGSARAAAIVAM 364 L ++ P VK +PY D SK V ++ V KTL+E ++LV LV+ LFL + R I + Sbjct: 313 LAKY-FPTGVKYTIPY-DTSKFVKISIEEVVKTLIEAIVLVFLVMFLFLQNIRYTIIPTI 370 Query: 365 TIPLSLLTAFILMHHFKIPANLLSLGA--IDFGILVDGAIVLVESVLRLREERPQGEITG 422 +P++LL F + F N+L++ + GILVD AIV+VE+V R+ E +G Sbjct: 371 VVPVALLGTFATLLLFGFSINVLTMFGMVLAIGILVDDAIVVVENVERIMSE--EGLSPA 428 Query: 423 ADIQQLMRQLRPIFFGMLVIIIA-YLPL 449 ++ M Q+ G+ +++IA ++P+ Sbjct: 429 DATRKAMGQISGALIGITLVLIAVFIPM 456 >gi|300313244|ref|YP_003777336.1| multidrug efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1033 Score = 112 bits (280), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 120/434 (27%), Positives = 203/434 (46%), Gaps = 30/434 (6%) Query: 18 LVFLLAALYGWYCWNQLPLEAYPDIADVTSQVVTQVNGLAAEEVEQQITIPLERQIMGTP 77 +V L L+G +QLP+ YP +A T + G + + + +ER++ G Sbjct: 14 VVALFIVLFGVIAMSQLPIARYPSVAPPTVSINATYPGATPQTMNDGVISLIERELSGVK 73 Query: 78 G-MHVMRSESTFGLSLITVVFNDGAEDYWSRQRLQERIAAVS--LPYGA-QPGLDALTSP 133 ++ S T G + I+V F G + ++ +Q ++ A+ LP Q G+ ++ Sbjct: 74 NLLYFESSADTSGTAQISVTFKPGTDPELAQVDVQNKLKAIEARLPQTVRQNGVTVESAA 133 Query: 134 IGEIYRYTLQS--KTRDLRELSELQFWKVIPRLKQVSGVVDVSNFGGLTTQFMLQLDPAK 191 + +L S D L++ ++ L+++ GV V FG + LDP K Sbjct: 134 SSFLLMVSLTSVDGRYDEVRLNDYLARNIVEELRRMEGVGRVQLFGA-EQAMRIWLDPFK 192 Query: 192 LVKYNTSLSQISQAISANNANA-----GGSIMKRGEQ-GLVIRGVGLIRSLDDLGNIVVS 245 L Y +++ IS AIS NA G + RG+Q + + G ++S IV+ Sbjct: 193 LNAYGLTVTDISTAISQQNAQIAPGRLGDAPAVRGQQIAMPLTVQGQLQSPAQFAAIVLK 252 Query: 246 QK-NGVPVLVKDLGQVVLGNQERHGVLGIDGQSDAIEGITLLLKNENPSRVMEGVHAAVQ 304 K +G VL+ D+ +V LG Q +G+ + + L N R + A ++ Sbjct: 253 AKADGSRVLLGDVARVELGAQSYAFANRENGKPAGVAAVQ-LSPGANAVRTAAAIQARME 311 Query: 305 DLNEHILPKDVKI-VPYIDRSKLVDATVHTVGKTLVEGMLLVSLVLLLFLGSARAAAIVA 363 +L LP+ + +PY + + V ++ V +TL+E M+LV LV+ LFL + R I A Sbjct: 312 ELRPS-LPQGMAFSLPY-NTAPFVKISIQKVVQTLIEAMVLVFLVMYLFLQNVRYTVIPA 369 Query: 364 MTIPLSLLTAFILMHHFKIPANLLSLGA--IDFGILVDGAIVLVESVLRL--------RE 413 + P++LL +M N+L++ + GI+VD AIV+VE+V R+ RE Sbjct: 370 IVAPIALLGTLAVMQLAGYSVNVLTMFGMVLAIGIIVDDAIVVVENVERIMAHEGLGPRE 429 Query: 414 E--RPQGEITGADI 425 + GEITGA I Sbjct: 430 ATIKAMGEITGAII 443 >gi|300311798|ref|YP_003775890.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 110 bits (275), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 118/434 (27%), Positives = 195/434 (44%), Gaps = 33/434 (7%) Query: 6 VNLCFQRRGI-VQLVFLLAALYGWYCWNQLPLEAYPDIADVTSQVVTQVNGLAAEEVEQQ 64 V+ F +R I L+ + L G W LP+ P + T QV Q+ G + E + Sbjct: 3 VSAAFIKRPIGTTLLAIAILLVGVAVWPLLPVAPLPQVDFPTIQVTAQLPGGSPETMASN 62 Query: 65 ITIPLERQIMGTPGMHVMRSESTFGLSLITVVFNDGAEDYWSRQRLQERIAAVS--LPYG 122 + PLERQ G+ M S+ST G + IT+ F+ + +Q I A + LP Sbjct: 63 VAQPLERQFSLIAGLSQMTSQSTLGSTQITLQFDLNRNIDAAALDVQAAINASTGQLPAN 122 Query: 123 --AQPGLDALTSPIGEIYRYTLQSKTRDLRELSELQFWKVIPRLKQVSGVVDVSNFGGLT 180 P + I ++QS+ L +++ + ++ Q+SGV V+ G Sbjct: 123 LPNPPTFRKINPADSPIMIMSVQSEALPLTVVNDYADNILAQQISQISGVGLVNVNGQQK 182 Query: 181 TQFMLQLDPAKLVKYNTSLSQISQAISANNANAGGSIMKRGEQGLVIRGVGLIRSLDDLG 240 +Q+DPAKL SL I I+ N + Q + + + Sbjct: 183 PAVRIQVDPAKLATLGLSLEDIRGVIATTTVNQPKGTVDGARQAFTVYTNDQLLKAEQWN 242 Query: 241 NIVVSQKNGVPVLVKDLGQVVLG---------------NQERHGVLGIDGQSDAIEGITL 285 ++V++ +NG P+ VKD+G V G N H + +G+S I L Sbjct: 243 DMVLAWRNGAPIRVKDIGIAVDGPENNKIAAWAFAGAANTADHSI--TNGRS-----IVL 295 Query: 286 LLKNENPSRVMEGV---HAAVQDLNEHILPKDVKIVPYIDRSKLVDATVHTVGKTLVEGM 342 + + + V+E V AA+ L I P + + IDR++ + A+V V TLV + Sbjct: 296 AITKQPGANVIETVGRIKAAMPRLRAAI-PAAIHVNTLIDRTQTIRASVEDVEFTLVLTI 354 Query: 343 LLVSLVLLLFLGSARAAAIVAMTIPLSLLTAFILMHHFKIPANLLSLGA--IDFGILVDG 400 LV +V+ +FL + A I ++T+PL+++ +M+ + LSL A I G +VD Sbjct: 355 ALVVMVIFVFLRNVAATLIPSVTVPLAIMGTAGVMYVVGYSLDNLSLMALTIAVGFVVDD 414 Query: 401 AIVLVESVLRLREE 414 AIV++E++ R EE Sbjct: 415 AIVMLENIYRYVEE 428 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.139 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,410,862 Number of extensions: 55805 Number of successful extensions: 230 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 202 Number of HSP's successfully gapped: 11 Length of query: 517 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 428 Effective length of database: 1,198,455 Effective search space: 512938740 Effective search space used: 512938740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)