BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0028.p (225 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311799|ref|YP_003775891.1| two component heavy metal respo... 178 3e-46 gi|300312356|ref|YP_003776448.1| cobalt/zinc/cadmium resistance ... 158 3e-40 gi|300309361|ref|YP_003773453.1| response regulator for cobalt/z... 157 5e-40 gi|300313818|ref|YP_003777910.1| two component transcription reg... 136 2e-33 gi|300312680|ref|YP_003776772.1| two component response regulato... 132 3e-32 >gi|300311799|ref|YP_003775891.1| two component heavy metal response transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 230 Score = 178 bits (452), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 93/218 (42%), Positives = 147/218 (67%), Gaps = 2/218 (0%) Query: 1 MQLLLVEDDMKAARLLAQGLQEEAFAVTVAHSAEEALAL-LDRPFDVAIFDRMLPGIDGL 59 M++L+VED+ K + L +GL E+ AV +A + + L ++ +DV + D MLPG+DG Sbjct: 1 MKILIVEDEQKMSEYLCKGLTEQGCAVDLAANGVDGQHLAIEHDYDVIVLDVMLPGLDGF 60 Query: 60 ALCRRVRQSQVLWPILMLTARDALGEKVEGLHAGVDDYLAKPFAFEELLARLHALLRRPR 119 ++ R +R+ + P++MLTARD++ ++++GLH G DDYL KPF+F ELLARL AL RR R Sbjct: 61 SVLRALRKVKE-TPVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGR 119 Query: 120 QLQVLHLQLADLRIDLLAHEVMRDGQRLDLTQKEYAVLVLLVRHSGQVVSRQQLAEEVWH 179 + L++ DL+IDLL+ + R G R+DLT KE+A+L ++ R G+++S+ +AE VW Sbjct: 120 AQEPAQLRIGDLQIDLLSRKAFRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVWD 179 Query: 180 ADLIAIDNLMDVHIKNLRRKIEADGRPALIETLRGQGF 217 + + N+++V IK LR KI+A L+ T+RG G+ Sbjct: 180 MNFDSNTNVVEVAIKRLRAKIDAPFEQKLLHTVRGMGY 217 >gi|300312356|ref|YP_003776448.1| cobalt/zinc/cadmium resistance two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 223 Score = 158 bits (400), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 85/218 (38%), Positives = 136/218 (62%), Gaps = 2/218 (0%) Query: 1 MQLLLVEDDMKAARLLAQGLQEEAFAVTVAHSAEEALAL-LDRPFDVAIFDRMLPGIDGL 59 M +L++EDD K L +GL+E +AV +A + + L + L++ +D+ + D MLPG+DG Sbjct: 1 MAILVIEDDPKTGDYLRKGLRESGYAVDLARNGADGLHMALEQDYDLVVLDVMLPGLDGW 60 Query: 60 ALCRRVRQSQVLWPILMLTARDALGEKVEGLHAGVDDYLAKPFAFEELLARLHALLRRPR 119 + +R + L P+L LTARD + +++ GL G DDYL KPF+F EL+ R+ LLRR Sbjct: 61 QVMSALRSKRDL-PVLFLTARDHVDDRIRGLQLGADDYLVKPFSFTELVLRIRTLLRRGV 119 Query: 120 QLQVLHLQLADLRIDLLAHEVMRDGQRLDLTQKEYAVLVLLVRHSGQVVSRQQLAEEVWH 179 + ++ADL++D + H+V R G + LT KE+ +L LL++ G+ +SR +A ++W Sbjct: 120 TREAEVYEIADLQLDHVRHKVTRQGVNIALTNKEFMLLHLLIKRQGEALSRSVIASQIWD 179 Query: 180 ADLIAIDNLMDVHIKNLRRKIEADGRPALIETLRGQGF 217 + + N++DV IK LR KI+A LI T+R G+ Sbjct: 180 MNFDSDTNVVDVAIKRLRAKIDAPFDKKLIHTVRSVGY 217 >gi|300309361|ref|YP_003773453.1| response regulator for cobalt/zinc/cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 157 bits (398), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 89/220 (40%), Positives = 137/220 (62%), Gaps = 6/220 (2%) Query: 1 MQLLLVEDDMKAARLLAQGLQEEAFAVTVAHSAEEAL---ALLDRPFDVAIFDRMLPGID 57 M +LLVED+ K+ L +GL E F V + + L LD + + I D MLPG++ Sbjct: 1 MHILLVEDEPKSGDYLRRGLTESGFVVDWVQTGVDGLHNARTLD--YKLIILDVMLPGMN 58 Query: 58 GLALCRRVRQSQVLWPILMLTARDALGEKVEGLHAGVDDYLAKPFAFEELLARLHALLRR 117 G + R +R++ P+L LTARD + ++V+GL G DDYL KPFA+ ELLAR+ LLRR Sbjct: 59 GWDILRELRRTHDT-PVLFLTARDEVEDRVKGLELGADDYLVKPFAYTELLARVRTLLRR 117 Query: 118 PRQLQVLHLQLADLRIDLLAHEVMRDGQRLDLTQKEYAVLVLLVRHSGQVVSRQQLAEEV 177 +V ++ D+ +D+L +V R GQR+DLT KE+A+L +L+R G+V+SR Q+A +V Sbjct: 118 GPSREVDLIEYDDVVLDVLRRKVTRAGQRIDLTAKEFALLHMLIRRPGEVLSRPQIASQV 177 Query: 178 WHADLIAIDNLMDVHIKNLRRKIEADGRPALIETLRGQGF 217 W + + N++DV ++ LR K++ L+ T+ G G+ Sbjct: 178 WDMNFDSDTNVVDVAVRRLRAKLDEGFEKKLLHTVWGMGY 217 >gi|300313818|ref|YP_003777910.1| two component transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 224 Score = 136 bits (342), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 83/220 (37%), Positives = 136/220 (61%), Gaps = 4/220 (1%) Query: 1 MQLLLVEDDMKAARLLAQGLQEEAFAVTVAHSAEEALALL-DRPFDVAIFDRMLPGIDGL 59 M++LLVED ++ +R LA+ +++ V AH+ +A +LL + + + I D LP +DG+ Sbjct: 1 MRILLVEDHIELSRWLAKAMRDAHLTVECAHNGADADSLLLTQDYALVILDLTLPRMDGM 60 Query: 60 ALCRRVRQSQVLWPILMLTARDALGEKVEGLHAGVDDYLAKPFAFEELLARLHALLRRPR 119 + +R+R P+L+LTAR L ++V GL+ G DDYLAKPF EEL AR+ ALLRR + Sbjct: 61 EVLKRMRARGNRTPVLILTARGGLADRVSGLNMGADDYLAKPFELEELEARVKALLRRSQ 120 Query: 120 QLQVLHLQLADLRIDLLAHEVMRDGQRLDLTQKEYAVL-VLLVRHSGQVVSRQQLAEEVW 178 + + + L D ++ G+ L LT +E+AVL L+VRH G V +++L ++V+ Sbjct: 121 NNEAVTVSCGALSFDTVSRTFTYGGELLALTPREHAVLEALIVRH-GHTVPKEKLFQQVF 179 Query: 179 HADLIAIDNLMDVHIKNLRRKIEADGRPAL-IETLRGQGF 217 + A + ++++I LR+K+E +G + I TLRG G+ Sbjct: 180 TLEDNASMDAIEIYIHRLRKKLEHEGPGRVGITTLRGLGY 219 >gi|300312680|ref|YP_003776772.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 220 Score = 132 bits (331), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 88/227 (38%), Positives = 131/227 (57%), Gaps = 24/227 (10%) Query: 1 MQLLLVEDDMKAARLLAQGLQEEAFAVTVAHSAEEALALLDRPFDVAIF--DRMLPGIDG 58 M +LLVEDD L++ L F V ++ +AL+ + F+ A+ D LPGIDG Sbjct: 1 MNVLLVEDDPVLTDGLSRVLNSHGFTVHTVNNGNDALS---QRFNAAVLVLDIGLPGIDG 57 Query: 59 LALCRRVRQSQVLWPILMLTARDALGEKVEGLHAGVDDYLAKPFAFEELLARLHALLRR- 117 + RR+R + P+L+LTARD + ++V GL G DDYL KPFA EL+AR+ AL+RR Sbjct: 58 FEVLRRMRAAGNNTPVLLLTARDTIPDRVHGLELGADDYLVKPFATPELVARIKALIRRS 117 Query: 118 ---PRQLQV--LHLQLADLRIDLLAHEVMRDGQRLDLTQKEYAVLVLLVRHSGQVVSRQQ 172 P QL V L L A R D+ DG++++L+ +E+ VL L++H+ +VVS+QQ Sbjct: 118 APQPAQLTVGGLSLDNATKRADI-------DGRKIELSLREWTVLEYLMQHASRVVSKQQ 170 Query: 173 LAEEV--WHADLIAIDNLMDVHIKNLRRKIEADGRPALIETLRGQGF 217 + + V W D N ++V++ +R KI G I T+RG G+ Sbjct: 171 IIDAVLPWGEDFTI--NAVEVYVSRIRLKIADSG--VAIRTIRGFGY 213 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.140 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 648,098 Number of extensions: 24635 Number of successful extensions: 271 Number of sequences better than 1.0e-08: 37 Number of HSP's gapped: 239 Number of HSP's successfully gapped: 37 Length of query: 225 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 143 Effective length of database: 1,231,600 Effective search space: 176118800 Effective search space used: 176118800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 129 (54.3 bits)