BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0027.p (471 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312679|ref|YP_003776771.1| periplasmic sensor signal trans... 130 2e-31 gi|300313819|ref|YP_003777911.1| two component sensor histidine ... 121 1e-28 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 119 4e-28 gi|300309980|ref|YP_003774072.1| two component transmembrane sen... 117 2e-27 gi|300309362|ref|YP_003773454.1| transmembrane sensory transduct... 115 6e-27 >gi|300312679|ref|YP_003776771.1| periplasmic sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 130 bits (327), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 103/342 (30%), Positives = 177/342 (51%), Gaps = 21/342 (6%) Query: 131 EPLRMAAVPVKLGERRFAVLVAGSLDDADH----IVESAALLFGGLAVALLLALAVAGAI 186 EP+R+AA+ V++ V VA ++ D I+ S +L G L A + + VA + Sbjct: 125 EPVRIAAMQVRVKNGVIWVAVAETIRKRDRAHYTILLSFLVLDGALTFASIFVVLVA--V 182 Query: 187 LTG-RLFGTVERIAAQARRISDASLGERLAHPGSDDEIGHLVDTLNSMLARLQRAFELQR 245 G R +++R Q ++ R G+ E+ L+ +N ++AR+ + + Q+ Sbjct: 183 RRGLRPLKSLQRNLEQRDHGKLGAIDGR----GAPVELQPLIVAMNELMARINKGEQAQQ 238 Query: 246 NFTANAAHEIRSPLSRLRTEIELSLRRPRSVQEQVATLQSCLEEVAGLTSMVEELLQLAR 305 NF A+ AH++R+PL+ L+ ++EL + + + +L V + +LL LAR Sbjct: 239 NFMADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARSLAMMNSSVERMIRQSRQLLALAR 298 Query: 306 LDAGQ-ESRQPERIALDEVMREAIARWQGTAAARRIRLEL-LAPETVHASVGERAALL-V 362 + G ESR+ E ++LD+++ E++ + A +RI L L P + G+R L + Sbjct: 299 SEPGLFESRKLEELSLDKLVEESVQHFIEEADKKRIDLGFDLRPARLR---GDRFLLSDL 355 Query: 363 LNNLLDNAIKFSAPDSAVEIRVFAEGGGATLEVLDRGPGIASGDQPQLFERFYRGRASQQ 422 ++NL+DNA+++S P V +R E L V D GPGIA + +F+RFYR Sbjct: 356 IDNLIDNAVRYSPPHGTVTVRCLEEQDATVLSVEDSGPGIAPEHRELIFDRFYRA----N 411 Query: 423 GQTSGAGLGLAIVRAVLDGYGASITLSNRGDGMGAACRLHFP 464 + +G+GLGLAIVR + +G I++ + G G + FP Sbjct: 412 DKVAGSGLGLAIVREIAHDHGGVISVDCKEQGQGTIFTVRFP 453 >gi|300313819|ref|YP_003777911.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 485 Score = 121 bits (303), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 89/253 (35%), Positives = 130/253 (51%), Gaps = 13/253 (5%) Query: 222 EIGHLVDTLNSMLARLQRAFELQRNFTANAAHEIRSPLSRLRTEIELSLRRPRSVQEQVA 281 E+ LV +N + RLQ QR F A+A+H++R+PL+ L+T+ EL+LR R Q A Sbjct: 227 EMRPLVQAMNGYMGRLQALISAQRRFIADASHQLRTPLTVLKTQSELTLRELRREQVDAA 286 Query: 282 TLQSCLEEVAGLTS----MVEELLQLARLDAGQESRQPERIALDEVMREAIARWQGTAAA 337 L+ +E +A T + LL LAR + G ++L + R+ A + Sbjct: 287 ELRDLVEGMARTTDAAVHLANRLLTLARAEHGAAEGGTMPLSLSDAARQVALELSQQAVS 346 Query: 338 RRIRLELLAPETVHASVGERAALL--VLNNLLDNAIKFSAPDSAVEIRVFAEGGG----- 390 R I L E S+ A LL ++ NLLDNAI+++ V +RV A+ Sbjct: 347 REIGLAF--EEEGDVSIEANALLLHELMVNLLDNAIRYTPAGGHVTLRVRAQSSPGQPTV 404 Query: 391 ATLEVLDRGPGIASGDQPQLFERFYRGRASQQGQTSGAGLGLAIVRAVLDGYGASITLSN 450 A LEV D GPGI + ++ ++FE FYR A+Q G GLGL+IVR + + AS+TL Sbjct: 405 ALLEVEDSGPGIPAAEREKVFEPFYRAGATQHVNPGGTGLGLSIVREIAAMHRASVTLDA 464 Query: 451 RGDGMGAACRLHF 463 G G R+ F Sbjct: 465 PAQGSGLVVRIVF 477 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 119 bits (299), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 94/270 (34%), Positives = 147/270 (54%), Gaps = 34/270 (12%) Query: 214 LAHPGSDDEIGHLVDTLNSMLARLQRAFELQRNFTANAAHEIRSPLSRLRTEIELSLRRP 273 LA G EI L LN +LARL+ A E QR F A+AAHE+R+PL+ LR + +L+ Sbjct: 186 LADVGMPQEIQPLTHALNDLLARLKHATETQRAFVADAAHELRTPLTALRLQTQLA---E 242 Query: 274 RSVQEQ-----VATLQSCLEEVAGLTSMVEELLQLARLDAGQESRQPERIALDEV-MREA 327 R+V E+ A L++ LE T +V +LL LAR ++P+ ++ +V +R Sbjct: 243 RAVDEEERRAAFADLKNGLERT---THLVHQLLTLAR-------QEPDAMSFTQVDLRAL 292 Query: 328 IARWQG--TAAARRIRLELLAPET------VHASVGERAALLVLNNLLDNAIKFSAPDSA 379 + G ++ A ++L + + + A ++ NNL+DNA++++ Sbjct: 293 LGSVVGDLSSVAEHGGIDLGLSDQGDDGRPLRVTGNADALRILFNNLVDNALRYTPAGGV 352 Query: 380 VEIRV-FAEGGGATLEVLDRGPGIASGDQPQLFERFYRGRASQQGQTS----GAGLGLAI 434 V++ V + G T+ V D GPGIA + P++F+RFYR + SQ+G S G+GLGLAI Sbjct: 353 VDVGVDLSTAGYCTVVVQDSGPGIAESELPRVFDRFYRAQHSQEGSGSRAAFGSGLGLAI 412 Query: 435 VRAVLDGYGASITLSNRGDGMGAACRLHFP 464 VR + D + + L N G G+ A R+ FP Sbjct: 413 VRQIADLHRVPVELRNTGHGLQA--RVVFP 440 >gi|300309980|ref|YP_003774072.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 390 Score = 117 bits (292), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 89/242 (36%), Positives = 135/242 (55%), Gaps = 20/242 (8%) Query: 221 DEIGHLVDTLNSMLARLQRAFELQRNFTANAAHEIRSPLSRLRTEIELSLRRPRSVQEQV 280 +EI LV +LN MLARL + +Q+ F A+AAH++++PL+ +R + EL+LR Q+ Sbjct: 126 EEISPLVRSLNDMLARLSQTIAIQKRFIADAAHQMKTPLAGMRMQSELALR-----QDDQ 180 Query: 281 ATLQSCLEEVAG----LTSMVEELLQLARLDAGQ-ESRQPERIALDEVMREAIARWQGTA 335 ++ LE++A T +V +LL LAR + Q +S + L+E+ R + W + Sbjct: 181 VDVRRSLEQLAKSSETATRLVNQLLSLARAENQQSQSGGMIPVELNELARSVVQDWVPMS 240 Query: 336 AARRIRLELLAPETVHASVGERAALL-VLNNLLDNAIKFSAPDSAVEIRVFA---EGGG- 390 +RI L P+ G L +L+NLLDNA+ ++ D + V A EGGG Sbjct: 241 FTQRIDLGFEQPDHPILITGNPVMLRELLSNLLDNALHYTPHDGNGRVTVRARTDEGGGL 300 Query: 391 ATLEVLDRGPGIASGDQPQLFERFYR--GRASQQGQTSGAGLGLAIVRAVLDGYGASITL 448 A LEV D GPGI ++ +FERFYR G S SG GLGLAIVR + + A++ + Sbjct: 301 AILEVEDNGPGIPVAERANVFERFYRILGTPS---TGSGLGLGLAIVREIAQQHEAALEI 357 Query: 449 SN 450 ++ Sbjct: 358 TS 359 >gi|300309362|ref|YP_003773454.1| transmembrane sensory transduction histidine kinase for cobalt/zinc/ cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 115 bits (289), Expect = 6e-27, Method: Compositional matrix adjust. Identities = 108/385 (28%), Positives = 191/385 (49%), Gaps = 11/385 (2%) Query: 83 LVQIIDADGQVLARSNNLGAGTLPVSADLLKRLKGGQTVFETLPKASEEPLRMAAVPV-K 141 LV+I ADG L S++ P DL + ++ +A+ PV + Sbjct: 86 LVRIARADGSTLLESHD--PTRTPPGIDLSQVDIRAWDSIQSWKLGDLRARYVASKPVTR 143 Query: 142 LGERRFAVLVAGSLDDADHIVESAALLFGGLAVALLLALAVAGAILTGRLFGTVERIAAQ 201 GE+ ++V + D + E + ++ V LLA+ ++ ++ L + +AAQ Sbjct: 144 SGEQAVVLVVRTASDRMLLLAEYRSDVWWAAGVGALLAILMSYFLVRSGL-RPLRTMAAQ 202 Query: 202 ARRISDASLGERLAHPGSDDEIGHLVDTLNSMLARLQRAFELQRNFTANAAHEIRSPLSR 261 R +S L RL + E+ +V++ N ML RL R+F+ F+A+ AH++R+PL+ Sbjct: 203 TRAVSANRLDTRLDLQAAPRELHEIVESFNEMLDRLHRSFQQLSQFSADLAHDVRTPLNN 262 Query: 262 LRTEIELSLRRPRSVQEQVATLQSCLEEVAGLTSMVEELLQLARLDAGQESRQPERIALD 321 L + +++L +PR + E A L S +EE L+ M+E +L LAR + Q + + + Sbjct: 263 LMVQTQVALGKPRGIDEYQALLSSNIEEYERLSRMMESMLFLARAENAHVVLQWQELDVA 322 Query: 322 EVMREAIARWQGTAAARRIRLELLAPETVHASVGERAALL--VLNNLLDNAIKFSAPDSA 379 + + ++G A ++RL + + A A LL + NL+ NAI+++ D Sbjct: 323 QELTRIADYFEGIADEAQVRLVVQGAGRLQAD----ATLLRRAVGNLVANAIRYTPADGV 378 Query: 380 VEIRVFAEGGGATLEVLDRGPGIASGDQPQLFERFYRGRASQQGQTSGAGLGLAIVRAVL 439 + ++V A G G + V + G GI + +LF+RFYR S+ +GLGLAIVR+++ Sbjct: 379 IRLQVDAAGDGMEISVSNPGAGIPAEQLERLFDRFYRADPSRSSAGGSSGLGLAIVRSIM 438 Query: 440 DGYGASITLSNRGDGMGAACRLHFP 464 +G ++++ G L FP Sbjct: 439 MLHGGQASVTS-SPGQLTVFSLRFP 462 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,304,179 Number of extensions: 51545 Number of successful extensions: 381 Number of sequences better than 1.0e-08: 37 Number of HSP's gapped: 348 Number of HSP's successfully gapped: 37 Length of query: 471 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 382 Effective length of database: 1,198,455 Effective search space: 457809810 Effective search space used: 457809810 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)