BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0020.p (223 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311750|ref|YP_003775842.1| transcription response regulato... 300 9e-83 gi|300313680|ref|YP_003777772.1| two component response regulato... 235 3e-63 gi|300309981|ref|YP_003774073.1| two component response regulato... 196 2e-51 gi|300311799|ref|YP_003775891.1| two component heavy metal respo... 163 9e-42 gi|300309361|ref|YP_003773453.1| response regulator for cobalt/z... 160 1e-40 >gi|300311750|ref|YP_003775842.1| transcription response regulator protein [Herbaspirillum seropedicae SmR1] Length = 220 Score = 300 bits (767), Expect = 9e-83, Method: Compositional matrix adjust. Identities = 165/220 (75%), Positives = 194/220 (88%) Query: 1 MRILLVEDDAMIGEEVRAALREASYAADWVRDGNAAHAALDQQDYDAVLLDLGLPGADGL 60 MRILLVEDDAMIGEEVR AL++ YAADWVR G+AA AL QQ YDA+LLDLGLPGADGL Sbjct: 1 MRILLVEDDAMIGEEVRCALKDNGYAADWVRAGDAALLALAQQQYDALLLDLGLPGADGL 60 Query: 61 SVLRQMRRHATTPVLILTARDGVQDRIDGLDSGADDYILKPFAMGELLARLRAVMRRKSG 120 +VLRQ+R H+ PVL++TARDG+QDRI GLD+GADDY+LKPFA+GELLARLRAV+RR+SG Sbjct: 61 AVLRQLRTHSLLPVLVITARDGIQDRIGGLDAGADDYLLKPFAIGELLARLRAVLRRRSG 120 Query: 121 AATVVLDNGELSLDLADKTARWQGQPPVALSAREFALLQALMHRPGAILSRSELEERIYG 180 AA+ L NG+++LDLA KTA W+ P V LSAREFALL ALM RPGAILSR+ELEE++YG Sbjct: 121 AASDQLSNGQITLDLAAKTACWREGPAVLLSAREFALLCALMGRPGAILSRAELEEKLYG 180 Query: 181 WGEEVESNAVEFLIHALRKKLDRTAIRNVRGMGWMVARGA 220 WGEEVESNAVEFLIH+LR+KLD+ AI+NVRGMGW+V R + Sbjct: 181 WGEEVESNAVEFLIHSLRRKLDKEAIKNVRGMGWLVQRAS 220 >gi|300313680|ref|YP_003777772.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 218 Score = 235 bits (599), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 122/217 (56%), Positives = 158/217 (72%), Gaps = 2/217 (0%) Query: 1 MRILLVEDDAMIGEEVRAALREASYAADWVRDGNAAHAALDQQDYDAVLLDLGLPGADGL 60 MR+LLVEDD M+GE VR LR+ +A DWV+DG +A AL +DY +LLDLGLP DGL Sbjct: 1 MRVLLVEDDPMVGEAVRKGLRQDGFAIDWVQDGKSADVALRTEDYAMLLLDLGLPQKDGL 60 Query: 61 SVLRQMR-RHATTPVLILTARDGVQDRIDGLDSGADDYILKPFAMGELLARLRAVMRRKS 119 +VLR +R R PVLI TARD V DR+ GLD+GADDY++KPF + EL AR+RA+ RR++ Sbjct: 61 AVLRTLRERGNAIPVLITTARDAVADRVAGLDAGADDYLIKPFDLEELSARMRALSRRQA 120 Query: 120 GAATVVLDNGELSLDLADKTARWQGQPPVALSAREFALLQALMHRPGAILSRSELEERIY 179 G A ++ E+ L+ A G+P V LSAREF LLQA M RPG +LSR++LEE++Y Sbjct: 121 GRAESLVQVREVILNPATHEVTVSGKP-VNLSAREFTLLQAFMDRPGVVLSRAQLEEKLY 179 Query: 180 GWGEEVESNAVEFLIHALRKKLDRTAIRNVRGMGWMV 216 GW + +ESNAVE IHALRKK+ I+NVRG+G++V Sbjct: 180 GWDDSIESNAVEVHIHALRKKVGSDFIKNVRGVGYLV 216 >gi|300309981|ref|YP_003774073.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 237 Score = 196 bits (497), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 103/221 (46%), Positives = 151/221 (68%), Gaps = 4/221 (1%) Query: 1 MRILLVEDDAMIGEEVRAALREASYAADWVRDGNAAHAALDQQDYDAVLLDLGLPGADGL 60 MRILL EDD+++ + + +LR + YAAD V++G A +AL QD+D ++LDLGLP GL Sbjct: 1 MRILLAEDDSVLADGLTRSLRHSGYAADCVKNGVEADSALSTQDFDLLILDLGLPKMSGL 60 Query: 61 SVLRQMR-RHATTPVLILTARDGVQDRIDGLDSGADDYILKPFAMGELLARLRAVMRRKS 119 VLR++R R++ PVLILTA D V+ R+ GLD GADDY+ KPFA+ EL AR+RA+ RR + Sbjct: 61 EVLRRLRARNSRLPVLILTAADSVEQRVQGLDLGADDYMAKPFALSELEARVRALTRRGA 120 Query: 120 GAATVVLDNGELSLDLADKTARWQGQPPVALSAREFALLQALMHRPGAILSRSELEERIY 179 G V+ +G LS D + A + G+ + LSARE LL+ L+ R G ++S+ +L + + Sbjct: 121 GGGPTVIRHGPLSYDQVGRIA-YIGEQMLELSARELGLLEVLLQRTGRLVSKEQLVDHLC 179 Query: 180 GWGEEVESNAVEFLIHALRKKLDRTAIR--NVRGMGWMVAR 218 WGEEV +NA+E +H LRKK++ +R VRG+G+ + + Sbjct: 180 EWGEEVSNNAIEVYVHRLRKKIEVGGVRIATVRGLGYCLEK 220 >gi|300311799|ref|YP_003775891.1| two component heavy metal response transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 230 Score = 163 bits (413), Expect = 9e-42, Method: Compositional matrix adjust. Identities = 95/227 (41%), Positives = 140/227 (61%), Gaps = 7/227 (3%) Query: 1 MRILLVEDDAMIGEEVRAALREASYAADWVRDG-NAAHAALDQQDYDAVLLDLGLPGADG 59 M+IL+VED+ + E + L E A D +G + H A+ + DYD ++LD+ LPG DG Sbjct: 1 MKILIVEDEQKMSEYLCKGLTEQGCAVDLAANGVDGQHLAI-EHDYDVIVLDVMLPGLDG 59 Query: 60 LSVLRQMRRHATTPVLILTARDGVQDRIDGLDSGADDYILKPFAMGELLARLRAVMRRKS 119 SVLR +R+ TPV++LTARD V+DRI GL GADDY++KPF+ ELLARL+A+ RR Sbjct: 60 FSVLRALRKVKETPVIMLTARDSVEDRIKGLHDGADDYLIKPFSFLELLARLQALTRRGR 119 Query: 120 GAATVVLDNGELSLDLADKTARWQGQPPVALSAREFALLQALMHRPGAILSRSELEERIY 179 L G+L +DL + A ++G + L+A+EFALL + R G ILS++ + E ++ Sbjct: 120 AQEPAQLRIGDLQIDLLSRKA-FRGGLRIDLTAKEFALLAVMARRQGEILSKTAIAELVW 178 Query: 180 GWGEEVESNAVEFLIHALRKKLD----RTAIRNVRGMGWMVARGASA 222 + +N VE I LR K+D + + VRGMG+++ A+A Sbjct: 179 DMNFDSNTNVVEVAIKRLRAKIDAPFEQKLLHTVRGMGYVLEPRATA 225 >gi|300309361|ref|YP_003773453.1| response regulator for cobalt/zinc/cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 160 bits (404), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 87/219 (39%), Positives = 134/219 (61%), Gaps = 5/219 (2%) Query: 1 MRILLVEDDAMIGEEVRAALREASYAADWVRDGNAAHAALDQQDYDAVLLDLGLPGADGL 60 M ILLVED+ G+ +R L E+ + DWV+ G DY ++LD+ LPG +G Sbjct: 1 MHILLVEDEPKSGDYLRRGLTESGFVVDWVQTGVDGLHNARTLDYKLIILDVMLPGMNGW 60 Query: 61 SVLRQMRRHATTPVLILTARDGVQDRIDGLDSGADDYILKPFAMGELLARLRAVMRRKSG 120 +LR++RR TPVL LTARD V+DR+ GL+ GADDY++KPFA ELLAR+R ++RR Sbjct: 61 DILRELRRTHDTPVLFLTARDEVEDRVKGLELGADDYLVKPFAYTELLARVRTLLRRGPS 120 Query: 121 AATVVLDNGELSLDLADKTARWQGQPPVALSAREFALLQALMHRPGAILSRSELEERIYG 180 +++ ++ LD+ + GQ + L+A+EFALL L+ RPG +LSR ++ +++ Sbjct: 121 REVDLIEYDDVVLDVLRRKVTRAGQ-RIDLTAKEFALLHMLIRRPGEVLSRPQIASQVWD 179 Query: 181 WGEEVESNAVEFLIHALRKKLD----RTAIRNVRGMGWM 215 + ++N V+ + LR KLD + + V GMG++ Sbjct: 180 MNFDSDTNVVDVAVRRLRAKLDEGFEKKLLHTVWGMGYV 218 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 660,826 Number of extensions: 25181 Number of successful extensions: 221 Number of sequences better than 1.0e-08: 39 Number of HSP's gapped: 203 Number of HSP's successfully gapped: 40 Length of query: 223 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 141 Effective length of database: 1,231,600 Effective search space: 173655600 Effective search space used: 173655600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)