BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0013.p (188 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane ... 223 1e-59 gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane ... 120 6e-29 gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane ... 101 4e-23 gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [H... 83 2e-17 gi|300312132|ref|YP_003776224.1| Fe2+-dicitrate sensor membrane ... 76 2e-15 >gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 223 bits (567), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 117/188 (62%), Positives = 138/188 (73%) Query: 1 VQLQFDADQRLITLERGEIFIDTGRDAISPRHRPFLVQTAQARLQALGTRFAVRQQAGVT 60 VQL+FD +RL+ L +GEIFIDTG DA P HRPF VQTA ARLQALGTRF VRQ T Sbjct: 155 VQLRFDDTRRLLLLRQGEIFIDTGADAQWPHHRPFWVQTAHARLQALGTRFDVRQHGDGT 214 Query: 61 RLSLLEGRVAVDTGQGTRTVIVPGETVEIDAGGNLRRQATAAAAMDPTAWLDGTLVTRDM 120 +L++LEGRVAV V PGE V+I G L R+A A+AMDPTAWL+ +LV R M Sbjct: 215 QLTVLEGRVAVHVAGVDPLVAQPGEIVDIHPDGRLVRRAARASAMDPTAWLEQSLVVRQM 274 Query: 121 RLGDVVAEMARYRHGWLYCEDGVASLKVSGVFQLRDGEAALESLAQALPVRIEQRTRFWV 180 RL DVVA++ARYR GWL C+ VA +VSGVFQL+D + ALE+LAQALP+ IE+RTRFWV Sbjct: 275 RLADVVAQLARYRSGWLQCDPAVADWQVSGVFQLQDTDVALEALAQALPLTIERRTRFWV 334 Query: 181 RVTALNSR 188 RV A R Sbjct: 335 RVAAAGKR 342 >gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 326 Score = 120 bits (301), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 73/187 (39%), Positives = 101/187 (54%), Gaps = 13/187 (6%) Query: 1 VQLQFDADQRLITLERGEIFIDTGRDAISPRHRPFLVQTAQARLQALGTRFAVRQQAGVT 60 + L F QRL+ L+RGEI + T D P RPFLV T Q L+ LGTRF VR++ Sbjct: 141 ITLDFTPAQRLLRLQRGEIMVLTAPDRQQP-PRPFLVATRQGSLRPLGTRFTVRERDQAV 199 Query: 61 RLSLLEGRVAVDTGQGTRTVIVPGETVEIDAGGNLRRQATAAAAMDPT-----AWLDGTL 115 +++L+G V + + ++P +G R + +AA P AW G L Sbjct: 200 EVAVLQGAVEITPASSAQQTVLP-------SGMQTRFTRSGSAAPRPCDDTQAAWSRGLL 252 Query: 116 VTRDMRLGDVVAEMARYRHGWLYCEDGVASLKVSGVFQLRDGEAALESLAQALPVRIEQR 175 + M L + E+ RYR G L + +A L+VSGVF L D + L LAQ LPVR++Q Sbjct: 253 IAERMPLAQFIGELGRYRSGLLQADPAIAGLEVSGVFPLADTDRVLRLLAQTLPVRVQQL 312 Query: 176 TRFWVRV 182 TR+WVRV Sbjct: 313 TRYWVRV 319 >gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 101 bits (252), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 69/183 (37%), Positives = 99/183 (54%), Gaps = 12/183 (6%) Query: 1 VQLQFDADQRLITLERGEIFIDTGRDAISPRHRPFLVQTAQARLQALGTRFAVRQQAGVT 60 V L F A++R + L GE+ + TG+ RPFLV+TAQ ALGTR+AVRQ+AG T Sbjct: 149 VNLAFSAEERRLRLISGELLVTTGKAGGG---RPFLVETAQGLAYALGTRYAVRQRAGRT 205 Query: 61 RLSLLEGRVAVDTGQGTRT--VIVPGETVEIDAGGNLRRQATAAAAMDPT--AWLDGTLV 116 +S+ EG V + Q + V+ G+ A G D T AW DG +V Sbjct: 206 EVSVWEGAVRLVPRQAAQQARVLQAGQQAAFSAQG-----VGDVTTADETRMAWTDGVIV 260 Query: 117 TRDMRLGDVVAEMARYRHGWLYCEDGVASLKVSGVFQLRDGEAALESLAQALPVRIEQRT 176 R MRL +AE+ RY L C V+ +++SG + + D +A + +LA AL ++ + R Sbjct: 261 ARGMRLDAFLAELGRYTTDRLSCAPAVSGVRISGSYPVADSDAVIATLAAALGLQAQLRQ 320 Query: 177 RFW 179 RFW Sbjct: 321 RFW 323 >gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 82.8 bits (203), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 61/182 (33%), Positives = 98/182 (53%), Gaps = 10/182 (5%) Query: 2 QLQFDADQRLITLERGEIFIDTGRDAISPRHRPFLVQTAQARLQALGTRFAVRQQAGVTR 61 FDA +R + L +GE+ ++ RD RPF++QTA ++ALGTRF VR+ T Sbjct: 156 NFHFDAQRRTVELVQGELLVEVARDPA----RPFVIQTADGSIRALGTRFLVRRDRAGTL 211 Query: 62 LSLLEGRVAVDTGQGTRT---VIVPGETVEIDAGGNLRRQATAAAAMDPTAWLDGTLVTR 118 +S+LE +V++ G T V+ G+ V + G + AAA++ AW LV+ Sbjct: 212 VSMLESKVSIQPGADTTQAPLVLEAGQRVRLHGGAAGPLEHIDAAALE-RAWRRHQLVSN 270 Query: 119 DMRLGDVVAEMARYRHG-WLYCEDGVASLKVSGVFQLRDGEAALESLAQALP-VRIEQRT 176 L +V+ E+ R+R G LY + +A L ++ V L D + AL+ L +P +R+ Q T Sbjct: 271 ARPLTEVLDELNRHRPGRILYDQAQLAGLHMTVVLPLDDTQRALQLLVDGVPGLRVRQFT 330 Query: 177 RF 178 + Sbjct: 331 PY 332 >gi|300312132|ref|YP_003776224.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 313 Score = 75.9 bits (185), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 46/170 (27%), Positives = 85/170 (50%), Gaps = 8/170 (4%) Query: 14 LERGEIFIDTGRDAISPRHRPFLVQTAQARLQALGTRFAVRQQAGVTRLSLLEGRVAVDT 73 +E G+I ++ + H+PF + +A+ L A RF+ + +++++G + T Sbjct: 150 MESGQILVNAMHN-----HKPFDILSARGNLHASAARFSFSSYETHSLVAVVQGSATLTT 204 Query: 74 GQGTRTVIVPGETVEIDAGGNLRRQATAAAAMDPTAWLDGTLVTRDMRLGDVVAEMARYR 133 G +I G+ V + G R + A A+ AW +G V D + DV+ + R+R Sbjct: 205 PDGMVKMITQGDVVSLSEAGIERSSQSLANAV---AWTEGLFVASDQAVADVIGVLRRHR 261 Query: 134 HGWLYCEDGVASLKVSGVFQLRDGEAALESLAQALPVRIEQRTRFWVRVT 183 G + A+ +VSG+F +R +A L LA+ + +++E RF V V+ Sbjct: 262 PGVIRVTRKAAAKRVSGIFDMRMPDAVLRQLAETVALKVENYGRFLVMVS 311 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.136 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 573,274 Number of extensions: 21928 Number of successful extensions: 108 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 101 Number of HSP's successfully gapped: 6 Length of query: 188 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 108 Effective length of database: 1,241,070 Effective search space: 134035560 Effective search space used: 134035560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 128 (53.9 bits)