BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0010.p (934 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311660|ref|YP_003775752.1| TonB-dependent receptor protein... 329 6e-91 gi|300313794|ref|YP_003777886.1| ferrichrome-iron receptor prote... 321 2e-88 gi|300311746|ref|YP_003775838.1| iron-regulated membrane protein... 277 3e-75 gi|300310824|ref|YP_003774916.1| hydroxamate-type ferrisideropho... 274 2e-74 gi|300311652|ref|YP_003775744.1| TonB-dependent siderophore rece... 267 3e-72 >gi|300311660|ref|YP_003775752.1| TonB-dependent receptor protein [Herbaspirillum seropedicae SmR1] Length = 705 Score = 329 bits (844), Expect = 6e-91, Method: Compositional matrix adjust. Identities = 225/625 (36%), Positives = 319/625 (51%), Gaps = 52/625 (8%) Query: 3 RPRLMVQALRLALLGLPAAAPAWAQTTTDATLPQVTVTAGQEEQATGPVHGFVAKRSATA 62 RP L A++ ALL L A A LPQV + A E A GPV+G+VAKR+A+A Sbjct: 5 RPTLTFLAVQCALLTLAAPA-----WAQQQALPQVEIKA-DAETANGPVNGYVAKRTASA 58 Query: 63 TKTDTPITETPQSISVVTADQIATQQARSVSEALGYSSGIQVSNGAFAISETASL----- 117 TKTDTP+ E PQS+SVVT D++ + S+ + L Y G A SET + Sbjct: 59 TKTDTPVLEVPQSVSVVTRDEMDARGVTSLLDVLRYMPG--------ANSETHGVDPRGY 110 Query: 118 ----LRGFPINNGGSLYRDGTLTASNASYSRYAPEPYGLERIELLHGPASVMFGQNAPGG 173 LRGF S + +G L + ++ E YG+ERIE+L GP SV FGQ PGG Sbjct: 111 EYFNLRGFINAQVTSNFLNG-LRQIPGGFGQFRTETYGMERIEVLRGPGSVTFGQADPGG 169 Query: 174 LINVVSKKPTTTTLRELQLQAGSFDRKQVAGDFGGALDEGGVWSYRITGLARDAGTQVD- 232 +IN VSK P EL + GSF+RKQV D G +G + +R+ GL D+ Q Sbjct: 170 VINRVSKLPGMDIPNELGVDIGSFNRKQVVADLNGHTQDGE-YLWRVVGLGLDSNNQYKF 228 Query: 233 ----GTVDRRLFLAAAITWRPSDRTEWTGRFEYQRSEGLSNNVFPASGTVTANPNGQIPT 288 + R +LA ++TWRPS T T +Y S TA G + T Sbjct: 229 NNGQSGDNNRFYLAPSLTWRPSASTSLTLMADYMNDRS-------GSSRWTAVRPGNVLT 281 Query: 289 STAVGRSADNSERYENWSLSSQFSHQFNEVWSFRQNLRYTDYNGTRNSMRFAAFYPATQS 348 T +G + + + WS QF H+F+ W+FRQN R + +AA P T + Sbjct: 282 HTLIGDPNFDRQSNDQWSFGYQFEHKFDNAWTFRQNFRQANLKSV-----YAALNPGTLT 336 Query: 349 ATAALQRWRL-GTESDTFTADNQLQADFSTGAVQHKVLMGVDFHHTAGTVRGYVSPNTLA 407 + + + ++ D DNQL+ GA +H VL G+D+ R Y Sbjct: 337 GSVLTRGTTVYNSQVDNTLVDNQLEGRLKWGATEHTVLFGLDWS------RMYDREVRYR 390 Query: 408 GYVLS-NLYTTSYG-NLPAVSENY-NLRTVDDQLGFYLQDQLKIAERWFVSAGIRHDRSQ 464 G S N+ YG +PA + + NL +Q G Y QDQ+K +R ++ G R D+ + Sbjct: 391 GAAPSLNIDNPVYGVTIPAATTLFGNLEENMNQTGLYAQDQIKFDQRLTLTLGGRWDKVK 450 Query: 465 QTVSNAIARTRTERSDRANTRNLGLVHELANGVSPYLSYTESFTPTTGTSFGGDMLKPER 524 N RT T D A + +GL + + ++PY+SY SF P TG+ F G P + Sbjct: 451 DETRNYFNRTTTRSDDSALSGRIGLSYLVTRDIAPYVSYATSFLPQTGSDFNGKPFDPTK 510 Query: 525 AKQYEAGVKYAPVNTNALFTLAVFDLRRQNVATTDLAHAGYSVQTGAARSKGVELEGKLS 584 A+Q E G+KY P + LFT A+F+L + NV T D AH ++V +G +S+G+ELE K Sbjct: 511 ARQAEVGIKYQPADGKTLFTAALFELTKTNVQTPDPAHTNFNVLSGEIKSRGLELEAKGE 570 Query: 585 LTEGLSLTSSYSYTNAKVTSNNATS 609 + +GL+L++SY+YT+ K T NN S Sbjct: 571 IVKGLNLSASYTYTDVKNTQNNTAS 595 >gi|300313794|ref|YP_003777886.1| ferrichrome-iron receptor protein [Herbaspirillum seropedicae SmR1] Length = 817 Score = 321 bits (822), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 235/628 (37%), Positives = 330/628 (52%), Gaps = 53/628 (8%) Query: 20 AAAPAWAQT-TTDATLP--QVTVTAGQEEQATGPVHGFVAKRSATATKTDTPITETPQSI 76 AA P+ A T +LP Q++ +A EQ + A+ SA+ TKT TP+ E PQSI Sbjct: 117 AATPSAATTPAASGSLPAIQISASADPGEQR------WTARDSASTTKTATPLIEVPQSI 170 Query: 77 SVVTADQIATQQARSVSEALGYSSGIQVSNGAFAISETASLLRGFPINNGGSLYRDGTLT 136 SVVTA++IA A + +ALGY+ G+ +S LRGF + G Y DG Sbjct: 171 SVVTAERIAAMGATTARDALGYTPGVNISPYGSDSRYDWINLRGFDAYSPG-FYLDGLPL 229 Query: 137 ASNASYSRYAPEPYGLERIELLHGPASVMFGQNAPGGLINVVSKKPTTTTLRELQLQAGS 196 + S++ + E YG+E++ +L GPASV++GQ PGG++NVVSK P+ RELQ+Q GS Sbjct: 230 RNANSFAVWRTENYGIEQLAVLRGPASVLYGQAGPGGVVNVVSKLPSADAPRELQVQLGS 289 Query: 197 FDRKQVAGDFGGALDEGGVWSYRITGLARDAGTQVDGTVDRRLFLAAAITWRPSDRTEWT 256 R+QV D GGA+D G YR+ G+ RDA G + RL+LA ++ W+ S T T Sbjct: 290 DRRRQVGADIGGAIDADGRLLYRVIGVVRDAELPAGGMKNDRLYLAPSLVWKISAATSLT 349 Query: 257 GRFEYQRSE-GLSNNVFPASGTVTANPNG-QIPTSTAVGRSADNSERYENWSLSSQFSHQ 314 + R + G+ P G++ G IP+ G + + + +H+ Sbjct: 350 LQAALLRDQGGVYTRTRPLVGSLVPTAIGTTIPSELYTGNPHFDHFSQDQELFGYRLAHR 409 Query: 315 FNEVWSFRQNLRY----TDYNGTRNSMRFAAFYPATQSATAALQ---RWRLGT-ESDT-F 365 F+E ++F Q+ R TDY + + RFA A + Q R G+ ES T F Sbjct: 410 FDERFAFTQSARVGHMSTDYRAVQ-TPRFATLNAANPNDPVNFQNLTRNLFGSRESATMF 468 Query: 366 TADNQLQADFSTGAVQHKVLMGVDFHHT---AGTVRGYVSPNTLAGYVLSNLYTTSYGNL 422 + DNQLQADFSTGAV+H VL GVD+ + A + G +P L N+ SY N Sbjct: 469 SIDNQLQADFSTGAVRHTVLAGVDYQRSRFDAVSFSGGSAP-------LLNIAAPSYPNG 521 Query: 423 PAV-----SENYNLRTVDDQLGFYLQDQLKIAERWFVSAGIRHDRSQQTVSNAIARTRTE 477 P V S+N R V Q G YLQDQ+K W V+ G RHD + TV N + +RT Sbjct: 522 PFVVPAPYSDN---RAVLTQTGIYLQDQIKWNNGWEVTLGGRHDSASSTVDNRLDGSRTS 578 Query: 478 RSDRANTRNLGLVHELANGVSPYLSYTESFTPT------TGTSFGGDMLKPERAKQYEAG 531 +DR TR +++ ANG++PYLSYTESF PT T SFG PE KQ E G Sbjct: 579 STDRKFTRRAAVLYHAANGLAPYLSYTESFNPTATVNPLTNQSFG-----PETGKQTEVG 633 Query: 532 VKYAPVNTNALFTLAVFDLRRQNVATTDLAHAGYSVQTGAARSKGVELEGKLSLTEGLSL 591 ++Y P TN L++ A+FDL+RQN + D Q G +G+ELE L L Sbjct: 634 LRYQPPGTNDLYSAALFDLKRQNYISYDANF--MPTQKGEIAVRGLELEAVTQPMAALRL 691 Query: 592 TSSYSYTNAKVTSNNATSSSTNTEGKAL 619 T++YSYT V + ++ + + A+ Sbjct: 692 TAAYSYTPRAVVTASSRPAEIGKQAAAV 719 >gi|300311746|ref|YP_003775838.1| iron-regulated membrane protein [Herbaspirillum seropedicae SmR1] Length = 435 Score = 277 bits (708), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 132/209 (63%), Positives = 156/209 (74%), Gaps = 14/209 (6%) Query: 709 VSTLSAWLCRFAPRRHHLVLLHRWTGLAIAFFLLVACLTGSLLSFEEELDTWLAPCCTAK 768 + +L WL R AP RH LVLLHRWTGL +AFFL+V LTGSLL F++ELD WLAP Sbjct: 5 LRSLRDWLVRHAPSRHVLVLLHRWTGLGMAFFLIVTSLTGSLLVFDDELDAWLAPQLQL- 63 Query: 769 IRHERLRRRCRYASTXAPGQAATAMLDPYQLRARVERALGPQAQVNQLILHPQPGRTVRF 828 + APGQ+A AM+DPY LR +V +A+GPQ ++QL+LH +PGRTV F Sbjct: 64 -------------AQPAPGQSAQAMMDPYLLREKVAQAMGPQVHISQLVLHARPGRTVSF 110 Query: 829 IAEPALDRSTGKPYRLSYDEVFANPYDGTVQGVRDRNAISLRPAQLMPFLFKVHHSMALP 888 A+PA D +TGKPYRL YDE+ NPY G VQGVRDR+ ISL+PA LMPFLFK+HHS+ALP Sbjct: 111 QADPATDPATGKPYRLGYDEILVNPYTGVVQGVRDRDKISLKPADLMPFLFKLHHSLALP 170 Query: 889 RVPGALLMGIVALLWTLDCFVGMILTWPR 917 R PGALL+G V LLWTLDCFVGMILTWPR Sbjct: 171 RAPGALLLGTVGLLWTLDCFVGMILTWPR 199 >gi|300310824|ref|YP_003774916.1| hydroxamate-type ferrisiderophore receptor signal peptide protein, tonB-dependent receptor [Herbaspirillum seropedicae SmR1] Length = 715 Score = 274 bits (701), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 195/593 (32%), Positives = 304/593 (51%), Gaps = 42/593 (7%) Query: 34 LPQVTVTAGQEEQATGPVHGFV-AKRSATAT---KTDTPITETPQSISVVTADQIATQQA 89 LP V+VTA G+ +K AT T K+ TP++ETPQSISVVT + +QQA Sbjct: 48 LPTVSVTA----------DGYTDSKFKATQTQVNKSSTPLSETPQSISVVTRAMMDSQQA 97 Query: 90 RSVSEALGYSSGIQVSNGAFAISETASLLRGFPINNGGSLYRDGTLTASNASYSRYAPEP 149 S+S+AL SG+ V+ ++RG ++ SLY DG T ++ SR A + Sbjct: 98 VSLSDALKNVSGV-VAQQYGRRGWDDLIIRGQVASD--SLYLDGLRTTAS---SRVAEQL 151 Query: 150 YGLERIELLHGPASVMFGQNAPGGLINVVSKKPTTTTLRELQLQAGSFDRKQVAGDFGGA 209 YGL+++E+L GP S+++G PGGL+N+VSK+P + + + GS D Q D + Sbjct: 152 YGLQQVEVLKGPGSLLYGLVLPGGLVNMVSKRPQGSDFANVDVTIGSHDLYQSTVDLNKS 211 Query: 210 LDEGGVWSYRITGLARDAGTQVDGTVDRRLFLAAAITWRPSDRTEWTGRFEYQRSEGLSN 269 L E G ++RI GL ++ D + ++A +++ RT++T YQ + Sbjct: 212 LSENGKQAFRINGLISNSDDATDHVWFKNRYIAPSLSLDLGARTDFTILTSYQERNYVRQ 271 Query: 270 NVFPASGTVTANPNGQIPTSTAVGRSADNSERYENWSLSSQFSHQFNEVWSFRQNLRYTD 329 P SG++T N +G + S +G + +H+F++ W+ NLRY Sbjct: 272 QGLPLSGSITPNASGPLARSLFIGEPGARPYAANETRIGYSLTHRFDDGWTLNNNLRYQQ 331 Query: 330 YNGTRNSMRFAAFYPATQSATAALQRWRLGTESDTFTADNQLQADFSTGAVQHKVLMGVD 389 + + + + + +A Q + E TFT D +Q F+T +H+V +G D Sbjct: 332 FTMSGQLISINNLTGTSLTRSAMDQTF----EGQTFTMDTNVQRSFATAWGKHEVTLGTD 387 Query: 390 FHHT---AGTVRGYVSPNTLAGYVLSNLYTTSYG-------NLPAVSENYNLRTVDDQLG 439 + ++ T+ V+P LSN+Y SYG L + + N +V D G Sbjct: 388 YMNSRELERTLTCSVTP-------LSNIYNPSYGARVVCPTQLTSTNAKLNDTSVRDT-G 439 Query: 440 FYLQDQLKIAERWFVSAGIRHDRSQQTVSNAIARTRTERSDRANTRNLGLVHELANGVSP 499 FY +D++ + ERW + AG+R DR+ ++A+ + ++ + A T + ++ EL +GV P Sbjct: 440 FYARDKISLGERWQLLAGVRQDRAATYTTSALTGSHSDNASSATTASAAIMVELWHGVRP 499 Query: 500 YLSYTESFTPTTGTSFGGDMLKPERAKQYEAGVKYAPVNTNALFTLAVFDLRRQNVATTD 559 YLSY SF P +GT+F G + KPE KQ+E GVK A+FDLRR+NV TD Sbjct: 500 YLSYATSFYPNSGTAFDGSIFKPETGKQWETGVKVDLDEGRTSINFALFDLRRRNVLVTD 559 Query: 560 LAHAGYSVQTGAARSKGVELEGKLSLTEGLSLTSSYSYTNAKVTSNNATSSST 612 H Y + G RS+G E+ L GLSL ++YSYT A V + S+T Sbjct: 560 PDHTSYKMAIGGQRSQGGEISVTSDLRNGLSLNAAYSYTAAIVIDDGGQRSTT 612 >gi|300311652|ref|YP_003775744.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 832 Score = 267 bits (682), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 204/604 (33%), Positives = 298/604 (49%), Gaps = 31/604 (5%) Query: 14 ALLGLPAAAPAWAQTTTDATLPQVTVTAGQEEQ-ATGPVHGFVAKRSATATKTDTPITET 72 AL L AP Q T L +TV+ ++E A PV G+VA RSATATKTDTP+ E Sbjct: 138 ALQPLVQGAPE--QVDTLHRLGNITVSGRRDEPGAADPVEGYVALRSATATKTDTPLLEL 195 Query: 73 PQSISVVTADQIATQQARSVSEALGYSSGIQV-SNGAFAISETASLLRGFPINNGGSLYR 131 QS+SVVT ++ + A S+ + L Y G ++G LRGF S Y Sbjct: 196 AQSVSVVTRAEMEARGANSILDVLRYMPGANTETHGVDPRGYDYFNLRGFINAQTTSNYL 255 Query: 132 DGTLTASNASYSRYAPEPYGLERIELLHGPASVMFGQNAPGGLINVVSKKPTTTTLRELQ 191 +G L + + + E YGLER+E+L G S FGQ PGG++N VSK + EL Sbjct: 256 NG-LRQIPSGFGMFRTETYGLERVEVLRGANSASFGQADPGGVVNRVSKLAGSGARNELM 314 Query: 192 LQAGSFDRKQVAGDFGGALDEGGVWSYRITGLARDAGTQVDGTVDR-----RLFLAAAIT 246 + GSF R+QVA D G LD G R+ GL D TQ R RL+LA ++ Sbjct: 315 VDVGSFQRRQVAADLSGDLDPEGRVQARLVGLLLDGDTQFRYANGRAGDNDRLYLAPSLK 374 Query: 247 WRPSDRTEWTGRFEYQRSEGLSNNVFPASGTVTANPNGQIPTSTAVGRSADNSERYENWS 306 RPS T EY + SG TA T T +G + +R E WS Sbjct: 375 IRPSADTSLILLAEYLKDRS-------GSGRWTAVRADGSQTHTLLGDPDFDQQRGEQWS 427 Query: 307 LSSQFSHQFNEVWSFRQNLRYTDYNGTRNSMRFAAFYPATQSATAALQRWRLGTESDTFT 366 L H+ + W+ RQ+ R ++ +F + S + A+ T+ + Sbjct: 428 LGWMLEHRLDATWTLRQHFRQAALRSQYAAVNPGSFNGSILSRSTAV----YDTQVENTL 483 Query: 367 ADNQLQADFSTGAVQHKVLMGVDFHHTAGTVRGY--VSPNTLAGYVLSNLYTTSYGNLPA 424 DNQ A G +H+VL+G+D+ H + + Y V+P+ + + +YT +PA Sbjct: 484 LDNQALARVQWGQAEHQVLLGLDWSHMSAREQRYRGVAPSL---DIDNPVYTQP---IPA 537 Query: 425 VSENY-NLRTVDDQLGFYLQDQLKIAERWFVSAGIRHDRSQQTVSNAIARTRTERSDRAN 483 + + +L Q G Y QDQ++ +R ++ G R+DRS+ + NA + + A Sbjct: 538 ATTRFGDLDQTLVQTGLYAQDQIRY-QRIVLTLGARYDRSRDSTRNAANNSLQAAEENAF 596 Query: 484 TRNLGLVHELANGVSPYLSYTESFTPTTGTSFGGDMLKPERAKQYEAGVKYAPVNTNALF 543 + +GL + ++ ++PY+SY SF P G G K A+Q EAGVKY P + A++ Sbjct: 597 SGRVGLSYLVSPELAPYVSYGSSFLPQAGQDVDGKPFKAATARQIEAGVKYQPGHGRAVY 656 Query: 544 TLAVFDLRRQNVATTDLAHAGYSVQTGAARSKGVELEGKLSLTEGLSLTSSYSYTNAKVT 603 T A++ L + N +D HA +SV G RS+GVELE K L GL++T++Y+Y T Sbjct: 657 TAALYQLVKDNALGSDPLHANFSVSNGQVRSRGVELEAKGELLPGLNVTAAYTYAQVVNT 716 Query: 604 SNNA 607 N A Sbjct: 717 QNAA 720 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.131 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,691,814 Number of extensions: 109584 Number of successful extensions: 396 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 336 Number of HSP's successfully gapped: 23 Length of query: 934 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 840 Effective length of database: 1,174,780 Effective search space: 986815200 Effective search space used: 986815200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 135 (56.6 bits)