BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0109.p (617 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312679|ref|YP_003776771.1| periplasmic sensor signal trans... 79 8e-16 gi|300312689|ref|YP_003776781.1| transmembrane sensor kinase tra... 67 3e-12 gi|300310848|ref|YP_003774940.1| two component sensor histidine ... 63 6e-11 gi|300313070|ref|YP_003777162.1| two component sensor histidine ... 63 8e-11 gi|300309980|ref|YP_003774072.1| two component transmembrane sen... 58 2e-09 >gi|300312679|ref|YP_003776771.1| periplasmic sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 79.3 bits (194), Expect = 8e-16, Method: Compositional matrix adjust. Identities = 76/277 (27%), Positives = 134/277 (48%), Gaps = 36/277 (12%) Query: 367 LASLRTQLGERNES--GALD-------LEEKIYLIQEAKSKLRKLEEQRNDALSFISHDL 417 L SL+ L +R+ GA+D L+ I + E +++ K E+ + + ++ ++H L Sbjct: 189 LKSLQRNLEQRDHGKLGAIDGRGAPVELQPLIVAMNELMARINKGEQAQQNFMADVAHQL 248 Query: 418 RSPLSNALLKLS------QDTEVDKQGVSML---IRRSLLLAEQFVGLFRAEST--DSKL 466 R+PL+ L+L QD + ++M+ + R + + Q + L R+E +S+ Sbjct: 249 RTPLTGLKLQLELLHDKHQDQPDTARSLAMMNSSVERMIRQSRQLLALARSEPGLFESRK 308 Query: 467 NESIDILAISYQAMDSVFEKLASKELRLGRSMPDEPLWVLGDFSLLERAFINLMDNAVNY 526 E + + + +++ E+ K + LG + P + GD LL NL+DNAV Y Sbjct: 309 LEELSLDKLVEESVQHFIEEADKKRIDLGFDL--RPARLRGDRFLLSDLIDNLIDNAVRY 366 Query: 527 SPRDSSIQV-TLEKRDTHVRFSVSNVGE-IGGEKRERLFQKYQRNLHEGGEANPMSNGIG 584 SP ++ V LE++D V SV + G I E RE +F ++ R +G+G Sbjct: 367 SPPHGTVTVRCLEEQDATV-LSVEDSGPGIAPEHRELIFDRFYR-----ANDKVAGSGLG 420 Query: 585 LFFVKVVAEKHGGEVGLDSEEG------IVRFWISLP 615 L V+ +A HGG + +D +E VRF ++ P Sbjct: 421 LAIVREIAHDHGGVISVDCKEQGQGTIFTVRFPLAPP 457 >gi|300312689|ref|YP_003776781.1| transmembrane sensor kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 459 Score = 67.4 bits (163), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 60/217 (27%), Positives = 112/217 (51%), Gaps = 21/217 (9%) Query: 414 SHDLRSPLS--NALLKLSQDTEVDKQGVSMLI------RRSLL-LAEQFVGLFRAESTDS 464 +H LR+PL+ ++ ++ ++ + D V+ ++ RS+ L + + L +AE++DS Sbjct: 245 AHQLRTPLAVIDSQIQFARQLD-DPARVAQVLASLQEGSRSMTDLTNKLLLLSQAEASDS 303 Query: 465 KL--NESIDILAISYQAMDSVFEKLASKELRLGRSMPDEPLWVLGDFSLLERAFINLMDN 522 +D+LA+S ++ + K + LG E V G+ + L +NL+DN Sbjct: 304 SAFPRAPVDLLAVSAMVLEELSGLALKKGIDLGLETTLESAMVNGNEAQLMGMLMNLIDN 363 Query: 523 AVNYSPRDSSIQVTLEKRDTHVRFSVSNVGE-IGGEKRERLFQKYQRNLHEGGEANPMSN 581 A+ Y+P + V LEK+ + + +VS+ G I E RER+F+++ RN + + Sbjct: 364 ALRYTPEGGKVTVGLEKKRSQMIITVSDNGPGIPAEVRERVFERFYRN----AAPDQPGS 419 Query: 582 GIGLFFVKVVAEKHGGEVGLDSE---EGIVRFWISLP 615 G+GL V+ + + G + LD+ G+V +SLP Sbjct: 420 GLGLAIVREIVQASQGSIRLDAPAQGSGLVA-TVSLP 455 >gi|300310848|ref|YP_003774940.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 465 Score = 63.2 bits (152), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 71/280 (25%), Positives = 128/280 (45%), Gaps = 28/280 (10%) Query: 348 VLVWNLGRVHLATQFMRQELASLRTQLGER------NESGALDLEEKIYLIQEAKSKLRK 401 +L W + R +AT+ +RQ LA ++LG E+G ++ + + ++++R+ Sbjct: 194 MLAWLVAR--MATRPIRQ-LAQAASRLGSDIDHAPLAETGPTEIRQAAHAFNAMQARIRR 250 Query: 402 LEEQRNDALSFISHDLRSPLSNALLKLSQDTEVDKQGVSMLIRRSLLLAEQFV--GLFRA 459 + R L+ I+HDL++PL+ L+L + V G+ + L + V GL A Sbjct: 251 QIQHRTHMLAAITHDLQTPLTRMRLRLEK---VSDPGLQQKLIDDLGVMHGMVREGLDLA 307 Query: 460 ESTDSKLNESIDILAISYQAMDSVFEKLASKELRLGRSMP---DEPLWVLGDFSLLERAF 516 S DS +E I L I DS+ + + + G+ + +V+ L R Sbjct: 308 RSMDS--SEKIQALDI-----DSLLDSVCADAADAGQDVALQGSTRAFVMAQPGALRRCL 360 Query: 517 INLMDNAVNYSPRDSSIQVTLEKRDTHVRFSVSNVGEIGGEKRERLFQKYQRNLHEGGEA 576 NL+DNA Y + + + + LE++ +R G I + E +F+ + R L Sbjct: 361 TNLIDNACKYG-KSARVSIALEQQKIAIRIRDQGPG-IPESELEAVFEPFYR-LEASRSR 417 Query: 577 NPMSNGIGLFFVKVVAEKHGGEVGLDS-EEGIVRFWISLP 615 + G+GL +AE H G++ L + EG + + LP Sbjct: 418 DTGGTGLGLTIALNIAENHHGQLRLRNLAEGGLEVLLELP 457 >gi|300313070|ref|YP_003777162.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 939 Score = 62.8 bits (151), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 68/241 (28%), Positives = 114/241 (47%), Gaps = 30/241 (12%) Query: 387 EKIYLIQEAKSKLRKLEEQR--NDALSFISHDLRSPLSNALLK---LSQDTEVDKQGVSM 441 E+++ + A+ L +E +R N LS ISHDLR+PL++ + L+ + +D +M Sbjct: 687 ERLHYAEVAQDALVTMESERLRNSLLSAISHDLRTPLTSLVGSADVLAANARLDDDARAM 746 Query: 442 LIRRSLLLAEQFVGLFRAESTDSKLNESIDILAISYQAMDSVFEKLASKELRL------G 495 R +++ VGL ++L + L + A+ E+LA LRL G Sbjct: 747 -ARTISEQSQKMVGLMNNLLDMARLQAGVVTLNRQWNAL----EELAGSALRLLAKALEG 801 Query: 496 RSM-----PDEPLWVLGDFSLLERAFINLMDNAVNYSPRDSSIQVT--LEKRD--THVRF 546 R + P+ PL + D LLER NL++NA+ YSP + I ++ +E D V+ Sbjct: 802 RQVDTRIAPELPLLRV-DAVLLERVLANLVENAIKYSPPGTPIGISAHVEHGDGAPQVKV 860 Query: 547 SVSNVGE-IGGEKRERLFQKYQRNLHEGGEANPMSNGIGLFFVKVVAEKHGGEVGLDSEE 605 V + G + F+K+ R +E A P G+GL + + E H G + + +E Sbjct: 861 CVMDQGRGFPPTMTQHAFEKFTRGDNES--ATP-GVGLGLAICRAIIEAHQGRIWIAPQE 917 Query: 606 G 606 Sbjct: 918 A 918 >gi|300309980|ref|YP_003774072.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 390 Score = 58.2 bits (139), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 51/205 (24%), Positives = 101/205 (49%), Gaps = 17/205 (8%) Query: 414 SHDLRSPLS----NALLKLSQDTEVD-KQGVSMLIRRS---LLLAEQFVGLFRAESTDSK 465 +H +++PL+ + L L QD +VD ++ + L + S L Q + L RAE+ S+ Sbjct: 157 AHQMKTPLAGMRMQSELALRQDDQVDVRRSLEQLAKSSETATRLVNQLLSLARAENQQSQ 216 Query: 466 LNE--SIDILAISYQAMDSVFEKLASKELRLGRSMPDEPLWVLGDFSLLERAFINLMDNA 523 +++ ++ + ++ + LG PD P+ + G+ +L NL+DNA Sbjct: 217 SGGMIPVELNELARSVVQDWVPMSFTQRIDLGFEQPDHPILITGNPVMLRELLSNLLDNA 276 Query: 524 VNYSPRDSSIQVTLEKR-DTHVRFSVSNVGEIGG----EKRERLFQKYQRNLHEGGEANP 578 ++Y+P D + +VT+ R D ++ V + G +R +F+++ R L G + Sbjct: 277 LHYTPHDGNGRVTVRARTDEGGGLAILEVEDNGPGIPVAERANVFERFYRIL--GTPSTG 334 Query: 579 MSNGIGLFFVKVVAEKHGGEVGLDS 603 G+GL V+ +A++H + + S Sbjct: 335 SGLGLGLAIVREIAQQHEAALEITS 359 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,837,226 Number of extensions: 77686 Number of successful extensions: 341 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 338 Number of HSP's successfully gapped: 11 Length of query: 617 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 526 Effective length of database: 1,188,985 Effective search space: 625406110 Effective search space used: 625406110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)