BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0102.p (342 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313393|ref|YP_003777485.1| major facilitator superfamily p... 629 0.0 gi|300313884|ref|YP_003777976.1| phthalate permease of the major... 349 2e-97 gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 114 1e-26 gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 99 5e-22 gi|300310490|ref|YP_003774582.1| major facilitator superfamily p... 70 2e-13 >gi|300313393|ref|YP_003777485.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 629 bits (1623), Expect = 0.0, Method: Compositional matrix adjust. Identities = 313/339 (92%), Positives = 325/339 (95%) Query: 4 VLHAGATGWLSLAGLRALMGLTEAAAIPSGMKAVAEWFPDKEKSVAVGWFNSGTSLGAML 63 VLHAGATGWLSLAG RALMGLTEAAAIPSGMKAVAEWFPDKEKSVAVGWFNSGTSLGAML Sbjct: 90 VLHAGATGWLSLAGFRALMGLTEAAAIPSGMKAVAEWFPDKEKSVAVGWFNSGTSLGAML 149 Query: 64 APPLVIFLQLRYGWQSAFVVTGVLGFAWAALWFAFYRSPRDHAALSETERNTIVGGQQRP 123 APPLVIFLQ+RYGWQSAFVVTG+LGF WAALWFAFYRSPRDHAALS++ER TI+ GQ RP Sbjct: 150 APPLVIFLQIRYGWQSAFVVTGILGFVWAALWFAFYRSPRDHAALSDSERETIISGQLRP 209 Query: 124 VGAVRGKRPVKDVVTSRRFWAIALARFFAEPAWQTFSFWIPLYFVNERHLDLKSIAMFAW 183 V AVRGKRPVKDVV SRRFWAIALARFFAEPAWQTFSFWIPLYFVNERHLDLKSIAMFAW Sbjct: 210 VSAVRGKRPVKDVVRSRRFWAIALARFFAEPAWQTFSFWIPLYFVNERHLDLKSIAMFAW 269 Query: 184 LPFLAADLGGLAGGYLSPLLMNRFKVPLIWSRVSGVVLGAVMMIGPACVGLVASPYTAIA 243 LPFLAADLGGLAGGYLSP L+NRF++PL+WSRV GVVLGAVMMIGPACVGLVASPY AIA Sbjct: 270 LPFLAADLGGLAGGYLSPFLINRFRIPLVWSRVCGVVLGAVMMIGPACVGLVASPYVAIA 329 Query: 244 LFCVGGFAHQMISGLVNTLSADVFDPEEVGTASGFAGMSAWIGGLGFSLLVGALADTVGY 303 LFCVGGFAHQMISGLVNTLSADVFDPEEVGTASGFAGMSAWIGGLGFSLLVGALADTVGY Sbjct: 330 LFCVGGFAHQMISGLVNTLSADVFDPEEVGTASGFAGMSAWIGGLGFSLLVGALADTVGY 389 Query: 304 GPLFGMLGAFDLIGAVLLIILIRGQSNDERAARTPPRTV 342 GPLFGMLGAFDLIGAVLLIILIRGQS +ERAAR+ +V Sbjct: 390 GPLFGMLGAFDLIGAVLLIILIRGQSTEERAARSLQSSV 428 >gi|300313884|ref|YP_003777976.1| phthalate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 418 Score = 349 bits (895), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 173/309 (55%), Positives = 220/309 (71%), Gaps = 1/309 (0%) Query: 7 AGATGWLSLAGLRALMGLTEAAAIPSGMKAVAEWFPDKEKSVAVGWFNSGTSLGAMLAPP 66 A ATGW SLA RAL+G+TEAA IP G+KA EWFP KE+SVA+GWFN G+S+GA+ APP Sbjct: 93 AFATGWQSLAFFRALLGITEAAGIPGGVKASTEWFPAKERSVAIGWFNIGSSIGALCAPP 152 Query: 67 LVIFLQLRYGWQSAFVVTGVLGFAWAALWFAFYRSPRDHAALSETERNTIVGGQQRPVGA 126 LV++ L GW+ +FVV G LG W LW FY+SPRD LS ER I+ GQ++ Sbjct: 153 LVVWTILHGGWKMSFVVVGALGVIWFVLWMLFYKSPRDQKLLSPEERAYILEGQEKSPEK 212 Query: 127 VRGKRPVKDVVTSRRFWAIALARFFAEPAWQTFSFWIPLYFVNERHLDLKSIAMFAWLPF 186 V+ + K +V SR FW+IA+ RF +EPAWQTF+ WIPLY ERH+++K IAMFAWLPF Sbjct: 213 VQRESWTK-IVRSRNFWSIAIPRFLSEPAWQTFNAWIPLYMATERHMNIKEIAMFAWLPF 271 Query: 187 LAADLGGLAGGYLSPLLMNRFKVPLIWSRVSGVVLGAVMMIGPACVGLVASPYTAIALFC 246 LAAD+G + GGYLSPL KV L SR +++G++ MIGPACVG V SPY AIAL Sbjct: 272 LAADIGCVLGGYLSPLFHKHLKVSLFTSRKLVMLVGSLSMIGPACVGFVDSPYVAIALLS 331 Query: 247 VGGFAHQMISGLVNTLSADVFDPEEVGTASGFAGMSAWIGGLGFSLLVGALADTVGYGPL 306 +GGFAHQ +SG + ++++DVF +V TA+G GMS ++G F+LL G L +GYGPL Sbjct: 332 IGGFAHQTLSGALYSITSDVFTKNQVATATGLTGMSGYLGATLFTLLFGILVTQIGYGPL 391 Query: 307 FGMLGAFDL 315 F +L AFDL Sbjct: 392 FVLLAAFDL 400 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 114 bits (286), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 83/313 (26%), Positives = 144/313 (46%), Gaps = 18/313 (5%) Query: 5 LHAGATGWLSLAG-------LRALMGLTEAAAIPSGMKAVAEWFPDKEKSVAVGWFNSGT 57 L AG T W+S A LR ++GL EA + P+ + VA WFP KE+ A FNS Sbjct: 95 LQAGVT-WMSTAAAVASLFLLRFMVGLAEAPSFPANGRIVAAWFPTKERGTASAIFNSAQ 153 Query: 58 SLGAMLAPPLVIFLQLRYGWQSAFVVTGVLGFAWAALWFAFYRSPRDHAALSETERNTIV 117 A+L PL+ ++ Y W F V GV+G A A +W SP+DH +++ E I Sbjct: 154 YFAAVLFTPLMAWIVHTYSWHHVFTVMGVVGIALALIWGRIIYSPKDHPRINQAELAHIE 213 Query: 118 GG------QQRPVGA--VRGKRPVKDVVTSRRFWAIALARFFAEPAWQTFSFWIPLYFVN 169 G R V A +G+ ++ ++++R + + ++ F W P+Y V Sbjct: 214 SGGGLVDMDNRKVAAPSTKGRGYIRQLLSNRMLLGVYIGQYCINVITYFFLTWFPVYLVQ 273 Query: 170 ERHLDLKSIAMFAWLPFLAADLGGLAGGYLSPLLMNRFKVPLIWSRVSGVVLGAVMMIGP 229 ER + + A +P + +GG+ GG +S L+ R + + W+R +V G ++ + Sbjct: 274 ERGMSILKAGFVASIPAVCGFIGGVVGGLISDRLIKR-GLSVTWARKIPIVGGMLLSMTM 332 Query: 230 ACVGLVASPYTAIALFCVGGFAHQMISGLVNTLSADVFDPEEVGTASGFAGMSAWIGGLG 289 V + + + + + F + + L + AD + VG + G M + G+ Sbjct: 333 ILCNYVEAQWMVVGIMALAFFG-KGVGALGWAVVADTAPKQIVGLSGGLFNMFGNVAGIT 391 Query: 290 FSLLVGALADTVG 302 +++G + G Sbjct: 392 TPIVIGYIVKETG 404 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 99.0 bits (245), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 67/219 (30%), Positives = 102/219 (46%), Gaps = 15/219 (6%) Query: 19 RALMGLTEAAAIPSGMKAVAEWFPDKEKSVAVGWFNSGTSLGAMLAPPLVIFLQLRYGWQ 78 R +GL EA P+G K V+EWF +E+ G F + +++G LAPP++ L L +GW+ Sbjct: 112 RVFLGLGEAPQFPAGAKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWR 171 Query: 79 SAFVVTGVLGFAWAALWFAFYRSPRDHAALSETERNTIVGGQQRP--VGAVRGKRPVKDV 136 FV+ GVLG A + W+ FYR+ R+ L+ +E + A K + Sbjct: 172 MMFVIMGVLGIAVSVGWYLFYRN-RNEVQLTPSEVLYLADDHDNDPNANAKLSWSEWKSL 230 Query: 137 VTSRRFWAIALARFFAEPAWQTFSFWIPLYFVNERHLDLKSIAMFAWLPFLAADLGGLAG 196 T R W + + W+P Y +ERHL + +P++ LG L+ Sbjct: 231 FTYRTTWGMLFGFMGVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSS 290 Query: 197 GYLSPLLMNRFKVPLIWSRVSGVVLGAVMMIGPACVGLV 235 GY++ LM R +P I SR P C GL+ Sbjct: 291 GYIADGLMKR-GMPAIRSRK-----------WPICAGLI 317 >gi|300310490|ref|YP_003774582.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 70.5 bits (171), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 80/325 (24%), Positives = 133/325 (40%), Gaps = 36/325 (11%) Query: 18 LRALMGLTEAAAIPSGMKAVAEWFPDKEKSVAVGWFNSGTSLGAMLAPPLVIFLQL---- 73 +R L+G+ EA P + +W P+ E+ A+ F SG+++ ++L+ P+ L L Sbjct: 118 MRFLLGVAEAGFFPGVVYYFTQWLPNHERGRAMAVFLSGSAIASILSGPITGALLLIEGG 177 Query: 74 -RYGWQSAFVVTGVLGFAWA-ALWFAFYRSPRDHAALSETERN---TIVGGQQ------R 122 +GWQ F++ G + +W +PR+ LS+ +R ++ +Q R Sbjct: 178 GLHGWQWMFIIEGGASMVFCVVVWLVLDSTPREAKWLSQEQREALAAVIAAEQEQRAANR 237 Query: 123 P--VGAVRGKRPVKDVVTSRRFWAIALARFFAEPAWQTFSFWIPLYFVNERHLDLKSIAM 180 P V A+ R + V+ ++AI L + A +FW+P H + + + Sbjct: 238 PAHVSALSLLRDPQIVLFCFIYFAIQLTIYGA-------TFWLPTIIKKMGHFNDFQVGL 290 Query: 181 FAWLPFLAADLGGLAGGYLSPLLMNRFKVPLIWSRVSGVVLGAVMMIGPACVGLVASPYT 240 F +P++ A +A YL +L R+K P W + V+ A M I VA P Sbjct: 291 FNSIPWIVA----IAAMYLFAVLAARYKKPQFWVATTLVISAAGMAISA-----VAGPVI 341 Query: 241 AIALFCVGGFAHQMISGLVNTLSADVFDPEEVGTASGFAGMSAWIGGLGFSLLVGALADT 300 C G + S L + D +GG G L Sbjct: 342 GFVAVCFAGIGFKAASSLFWPIPQAYLDARIAAAVLALINSLGNLGGFFAPATFGYLEQK 401 Query: 301 VG--YGPLFGMLGAFDLIGAVLLII 323 G G LF + GA L+ AVL+ + Sbjct: 402 TGSIQGGLFALSGA-SLLAAVLVFL 425 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.140 0.449 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,094,682 Number of extensions: 45134 Number of successful extensions: 285 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 275 Number of HSP's successfully gapped: 11 Length of query: 342 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 256 Effective length of database: 1,212,660 Effective search space: 310440960 Effective search space used: 310440960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)