BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0100.p (400 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313395|ref|YP_003777487.1| porin transmembrane protein [He... 627 0.0 gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) ... 143 2e-35 gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) ... 125 8e-30 gi|300310328|ref|YP_003774420.1| porin signal peptide protein [H... 123 3e-29 gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspir... 117 2e-27 >gi|300313395|ref|YP_003777487.1| porin transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 627 bits (1616), Expect = 0.0, Method: Compositional matrix adjust. Identities = 322/371 (86%), Positives = 342/371 (92%), Gaps = 5/371 (1%) Query: 35 AQSAVTLYGTVDLGLAYSSNQGGASNTYMNSGALNASKLGVMGSEDLGGGTSALFRLENG 94 AQS+VTLYGTVDLGLAYSSNQGGASNTYMNSGAL ASKLG+ G+EDLGGG ALFRLENG Sbjct: 33 AQSSVTLYGTVDLGLAYSSNQGGASNTYMNSGALAASKLGLTGTEDLGGGNMALFRLENG 92 Query: 95 FNADTGAMSSSGVLFNRQSYVGLASRDWGQITVGRQYTPYFQYVGGLGPTGVLTGATGAH 154 FNADTGA S++GVLFNRQSYVGL+S+D+GQ+TVGRQYTPYFQYVG LGPT VLTGATGAH Sbjct: 93 FNADTGAQSTAGVLFNRQSYVGLSSKDYGQVTVGRQYTPYFQYVGALGPTNVLTGATGAH 152 Query: 155 PGDIDALDTTLRMNNSVLYTLPTLGGLQAGIQYGMGEQAGSAASGSSVSAALRYDYQAFS 214 PGDIDALDTTLRMNNS+ YTLPTLGGLQAG+QYGMGEQAGSAA+GSSVSAALRYDYQAFS Sbjct: 153 PGDIDALDTTLRMNNSITYTLPTLGGLQAGVQYGMGEQAGSAANGSSVSAALRYDYQAFS 212 Query: 215 WSAGYTRLKNLANGNTAGSFGNVGSFANNSPINAGYASADSTQLLATAARYTWGDLMLGL 274 WSAGYTRLKNLANGNTAGSFGNVGSFANNSP+NAGYASADSTQLLATAARYT GDLMLGL Sbjct: 213 WSAGYTRLKNLANGNTAGSFGNVGSFANNSPVNAGYASADSTQLLATAARYTRGDLMLGL 272 Query: 275 NYSNVQYKPGALSAFAQTASFNTVGLISSYNVNAALTLAAGYSYTAEKARNGISSPAKYN 334 NYSNVQYK GALSAF+QTA FNT+GLI SYNV+ AL LAAGYSYTAEKARNGISSPAKYN Sbjct: 273 NYSNVQYKAGALSAFSQTAVFNTIGLIGSYNVSPALVLAAGYSYTAEKARNGISSPAKYN 332 Query: 335 QLSLEEVYALSKRTALYAXGGGGGGGG-----GGGGSSIVDAVAAVGDSQNGTPSSGQGQ 389 QLSLEEVY+LSKRTALYA G GG GSSIV+AVA+VGDSQNGTPSSG GQ Sbjct: 333 QLSLEEVYSLSKRTALYAIQAYQRASGQTLRAGGVGSSIVNAVASVGDSQNGTPSSGSGQ 392 Query: 390 FVAMLGLRHSF 400 FVAMLG+RHSF Sbjct: 393 FVAMLGIRHSF 403 >gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 352 Score = 143 bits (361), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 138/408 (33%), Positives = 182/408 (44%), Gaps = 81/408 (19%) Query: 16 QSLYLCAALLGAATSMPASAQSAVTLYGTVDLGLAYSSNQ-----GGASNTYMNSGALNA 70 ++L + A L G AT+ A AQS+V++YG +DLGL Y S+ GGA M+SG Sbjct: 3 KTLLVAAMLAGGATT--AHAQSSVSIYGLIDLGLTYYSSAPKSGGGGARLLRMDSGIAQG 60 Query: 71 SKLGVMGSEDLGGGTSALFRLENGFNADTGAMSSSGVLFNRQSYVGLASRDWGQITVGRQ 130 S++G G+EDLGGG +A F LE GF AD G++ G+LF RQS+VGL S WG ++VGRQ Sbjct: 61 SRIGFKGTEDLGGGMNAFFTLETGFGADDGSLGQGGLLFGRQSFVGLGSTQWGSLSVGRQ 120 Query: 131 YTPYFQYVGG------LGPTGVLTGATGAHPGDIDALDTTL----RMNNSVLYTLPTLGG 180 Y + +GG L P G A AL+ + R NNS+ Y GG Sbjct: 121 Y-DFMANIGGQYAMGALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAKFGG 179 Query: 181 LQAGIQYGMGEQAGSAASGSSVSAALRYDYQAFSWSAGYTRLKNLANGNTAGSFGNVGSF 240 G G+GE AG+ +G +VSA +YD FS + YT +K ++ GSF Sbjct: 180 FSFGAMVGLGETAGNNNAGRTVSATGQYDNGPFSGALAYTDIK-----DSTGSF------ 228 Query: 241 ANNSPINAGYASADSTQLLATAARYTWGDLM-LGLNYSNVQYKPGALSAFAQTASFNTVG 299 ST++ ARY G G+ Q K SA A T TV Sbjct: 229 --------------STRIYGGGARYQIGAFTPFGV---VTQVKNTNTSAKATTYELGTV- 270 Query: 300 LISSYNVNAALTLAAGYSYTAEKARNGISSPAKYNQLSLEEVYALSKRTALYAXGGGGGG 359 Y + LAA Y + +K G+ + L Y +SKRT LY Sbjct: 271 ----YALTPLWDLAAAYQF--QKRNQGVGNA---QALVATVDYKISKRTDLY-------- 313 Query: 360 GGGGGGSSIVDAVAAVGDSQNGTPSSGQG-------QFVAMLGLRHSF 400 V AV N P G G Q LG+RH F Sbjct: 314 ---------VGAVYDRDKGYNAYPVFGGGIQSATGNQTALRLGMRHRF 352 >gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 125 bits (313), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 113/342 (33%), Positives = 170/342 (49%), Gaps = 35/342 (10%) Query: 37 SAVTLYGTVDLGLAYSSN----QGGASNTY-MNSGALNASKLGVMGSEDLGGGTSALFRL 91 S+VT+YG VD G+ Y S GG + + +NSG + S++G G+EDLGGG SA+F L Sbjct: 22 SSVTIYGIVDTGVVYQSKVATPTGGTGSKFSLNSGVIQGSRIGFKGTEDLGGGLSAVFNL 81 Query: 92 ENGFNADTGAM---SSSGVLFNRQSYVGLASRDWGQITVGRQYTPYFQYVGGLGPTGVLT 148 E GF+ DTGA+ +S LF R+S VGLA ++G + +GRQ T + + Sbjct: 82 EAGFSNDTGALDDSKTSNTLFRRKSVVGLAG-NFGSVLLGRQ-TDFADTISSYTSVQDFG 139 Query: 149 GATGAHPGDIDALDTTLRMNNSVLYTLPTLGGLQAGIQYGMGEQAGSAASGSSVSAALRY 208 G TGA ++D L+ T R NNS+ YT L G + YG GE AG ++G + +Y Sbjct: 140 GWTGAAGHNLDRLEGT-RTNNSISYTTANLNGFTGNLMYGFGETAGQTSAGQAFGVGGKY 198 Query: 209 DYQAFSWSAGYTRLKNLANGNTAGSFGNVGSFANNSPINAGYASADSTQLLATAARYTWG 268 D Y + K G+T + + N N + + ++L AA Y +G Sbjct: 199 DNGPLGLGINYYQSK---AGSTPADASLLAATGTNQFTNGTNNGSTAQKVLNVAASYQFG 255 Query: 269 DLMLGLNYSNVQYK-----------PGALS-------AFAQTASFNTVGLISSYNVNAAL 310 + N+S V+ G+L+ A + A +G ++Y+++ +L Sbjct: 256 PARVYGNWSRVKQDLNTQANLTAAGVGSLATISNRTLAASNKADIYEIG--TAYSLSPSL 313 Query: 311 TLAAGYSYTAEKARNGISSPAKYNQLSLEEVYALSKRTALYA 352 L A +T + +G+S AK Q+SL Y LSKRT LYA Sbjct: 314 KLLAAVDHTRAQF-DGVSGKAKLTQVSLGTDYWLSKRTDLYA 354 >gi|300310328|ref|YP_003774420.1| porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 405 Score = 123 bits (308), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 119/377 (31%), Positives = 167/377 (44%), Gaps = 47/377 (12%) Query: 12 KRSLQSLYLCAALLGAATSMPASAQSAVTLYGTVDLGLAYSSN-----QGGASNTYMNSG 66 K+SL +L + A GAA + S++T+YG +D L Y++ + S T + SG Sbjct: 2 KKSLLALAVLGAFAGAAHAQ----SSSITIYGAIDASLTYTNKIATDGKNTGSLTAVESG 57 Query: 67 ALNASKLGVMGSEDLGGGTSALFRLENGFNADTGAMSSSGVLFNRQSYVGLASRDWGQIT 126 + S+LG+ GSEDLGGG A+F LENGFN DTG +++ RQ+ VGL S ++G + Sbjct: 58 LVKGSRLGIKGSEDLGGGLKAVFTLENGFNVDTGEAGQGKLMWGRQTTVGL-SGNFGTVL 116 Query: 127 VGRQYTPYFQYVGGLGPTGVLTGATGAHPGDIDALDTTLRMNNSVLYTLPTLGGLQAGIQ 186 VGRQ P +GG+ G H +D D R+NNS+ Y P GL Sbjct: 117 VGRQNDP-LNDIGGIASAGGFGVPGTVHSKGLDRTDGQ-RLNNSIRYNSPNFNGLSFSGI 174 Query: 187 YGMGEQAGSAASGSSVSAALRYDYQAFSWSAGYTRLKNLANGNTAGSFGNVGSFANNSPI 246 YG GEQ G A G S+ +Y S GY + K G + GS A N Sbjct: 175 YGFGEQVGRAVGGRSIGLGSQYVNGPLSLGLGYFQSK--MGGGSDGSSDVNAKAACNGAG 232 Query: 247 NAGYASADSTQLLATAARYTWGDLMLGLNYSNVQ-------------------------- 280 +A + AA Y +G + +S+V+ Sbjct: 233 EKDHAGDTCLKTWTLAAAYQFGPAKVYGAFSHVRLPLAKLHTKVRKDNNFGAMYDAKTGT 292 Query: 281 -YKPGALSAF----AQTASFNTVGLISSYNVNAALTLAAGYSYTAEKARNGISSPAKYNQ 335 Y P +AF A S T + SY + +LTL + +T K S + Q Sbjct: 293 GYNPSDATAFSIGGANHKSSQTFDIGMSYQLMPSLTLMSAVQHTRAKFVGAAS--GRLLQ 350 Query: 336 LSLEEVYALSKRTALYA 352 L+L Y LSKRT+ YA Sbjct: 351 LNLGAKYDLSKRTSAYA 367 >gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 374 Score = 117 bits (293), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 79/179 (44%), Positives = 105/179 (58%), Gaps = 8/179 (4%) Query: 33 ASAQSAVTLYGTVDLGLAYSSNQG--GASNTYMNSGALNASKLGVMGSEDLGGGTSALFR 90 A AQS VT+YG VD + Y SN G SN M++GA+ S++G G+EDLGGG A+F+ Sbjct: 19 AFAQSNVTIYGIVDTSIHYLSNANAQGNSNVRMDNGAIANSRIGFKGTEDLGGGLKAMFQ 78 Query: 91 LENGFNADTGAMSSSGVLFNRQSYVGLASRDWGQITVGRQYTPYFQYVGG-LGPTGVLTG 149 LENGF++DTGAM G LF RQS+VGL +G++ VGRQ TP F + P LT Sbjct: 79 LENGFSSDTGAMQQGGSLFGRQSWVGLQG-GFGKVRVGRQNTPLFDLLADHFDP---LTV 134 Query: 150 ATGAHPGDIDALDTTLRMNNSVLYTLPTLGGLQAGIQYGMGEQAGSAASGSSVSAALRY 208 A + A + +R N + Y G + A + GE+AGS +GS S+ALRY Sbjct: 135 GNYASNSWLPAAASLVRSPNMLRYD-GDFGPVAASASWAFGEKAGSVRTGSQFSSALRY 192 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.311 0.128 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,113,289 Number of extensions: 41794 Number of successful extensions: 186 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 147 Number of HSP's successfully gapped: 15 Length of query: 400 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 313 Effective length of database: 1,207,925 Effective search space: 378080525 Effective search space used: 378080525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 132 (55.5 bits)