BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0092.p (422 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313879|ref|YP_003777971.1| major facilitator superfamily p... 748 0.0 gi|300312616|ref|YP_003776708.1| major facilitator superfamily p... 419 e-118 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 93 3e-20 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 83 4e-17 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 82 6e-17 >gi|300313879|ref|YP_003777971.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 413 Score = 748 bits (1930), Expect = 0.0, Method: Compositional matrix adjust. Identities = 394/422 (93%), Positives = 408/422 (96%), Gaps = 9/422 (2%) Query: 1 MFSWYRQVTAKERKTFWACFSGWALDALDVQMFSLAIPALIAAFSLSKADAGMISGATLV 60 MFSWYRQVTAKERKTFWACFSGWALDALDVQMFSLAIPALIAAFSLSKADAG+ISGATLV Sbjct: 1 MFSWYRQVTAKERKTFWACFSGWALDALDVQMFSLAIPALIAAFSLSKADAGLISGATLV 60 Query: 61 ASAIGGWFAGALSDRYGRVKTLQITIIWFSFFTFLSAFAQSYPQLLVLKALQGFGFGGEW 120 ASAIGGWFAGALSDRYGRVKTLQITIIWFSFFTFLSAFAQS+PQLLVLKALQGFGFGGEW Sbjct: 61 ASAIGGWFAGALSDRYGRVKTLQITIIWFSFFTFLSAFAQSFPQLLVLKALQGFGFGGEW 120 Query: 121 AAGAVLMAETIRAEHRGRAMGTVQSAWAVGWGGAVLLFSVLFTMLPQEMAWRVMFVIGLL 180 AAGAVLMAETIRAEHRG+AMGTVQSAWAVGWGGAVLLFSVLF++LPQEMAWRVMFVIGLL Sbjct: 121 AAGAVLMAETIRAEHRGKAMGTVQSAWAVGWGGAVLLFSVLFSLLPQEMAWRVMFVIGLL 180 Query: 181 PALLVVYVRRSVSETDAFLAGRKAATSKPAASTLLGIFRPDVLRLTLIGSLLGVGAHGGY 240 PALLVVYVRRSV ETDAFLAG+KAA SKPAASTLLGIFRPDVLRLT+IGSLLGVGAHGGY Sbjct: 181 PALLVVYVRRSVQETDAFLAGQKAAASKPAASTLLGIFRPDVLRLTIIGSLLGVGAHGGY 240 Query: 241 YALMTWLPTYLKTEKHLSVLSTGGYLAVIIIAFWCGCIASAQLLDRIGRRRTVASFALCC 300 YALMTWLPTYLKTEKHLSVLSTGGYLAVII+AFWCGCIAS+QLLDRIGRRRTVA FAL C Sbjct: 241 YALMTWLPTYLKTEKHLSVLSTGGYLAVIIVAFWCGCIASSQLLDRIGRRRTVAFFALAC 300 Query: 301 VATVLCYIFLPLTDTQMLICGFPLGFFAAGIPASLGALFNELYPSGIRGTGVGFCYNFGR 360 VATVLCYIFLPL+DTQML+CGFPLGFFAAGIPAS+GALFNELYPSGIRGTGVGFCYNFGR Sbjct: 301 VATVLCYIFLPLSDTQMLVCGFPLGFFAAGIPASMGALFNELYPSGIRGTGVGFCYNFGR 360 Query: 361 VASAGFPVLVGHMSDSMPLGQAIGMRAFKSLGGIDAAIAYSLVVIAVLMLPETKGRQMET 420 VASAGFPVLVGHMSDSMPLGQAI GIDAAIAYSLVV+AVLMLPETKGR+MET Sbjct: 361 VASAGFPVLVGHMSDSMPLGQAI---------GIDAAIAYSLVVVAVLMLPETKGRKMET 411 Query: 421 GA 422 A Sbjct: 412 SA 413 >gi|300312616|ref|YP_003776708.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 414 Score = 419 bits (1078), Expect = e-118, Method: Compositional matrix adjust. Identities = 214/416 (51%), Positives = 284/416 (68%), Gaps = 9/416 (2%) Query: 4 WYRQVTAKERKTFWACFSGWALDALDVQMFSLAIPALIAAFSLSKADAGMISGATLVASA 63 W ++ T ER+T A F+G+A+D D +++ IP L+AA+S+SKA+AG I+ L+ SA Sbjct: 2 WLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTSA 61 Query: 64 IGGWFAGALSDRYGRVKTLQITIIWFSFFTFLSAFAQSYPQLLVLKALQGFGFGGEWAAG 123 IGGW AG L+DRYGRV+ LQ T++WF+FFTFLS F S+ QL +A+QGFG GGEWA G Sbjct: 62 IGGWAAGVLADRYGRVRVLQWTVLWFAFFTFLSGFTTSFGQLFFTRAMQGFGMGGEWAVG 121 Query: 124 AVLMAETIRAEHRGRAMGTVQSAWAVGWGGAVLLFSVLFTMLPQEMAWRVMFVIGLLPAL 183 +VL+AETI A HRG+A G VQS+WAVGW G+ L F ++ +LP E AW+V+F IG+LPAL Sbjct: 122 SVLIAETISARHRGKASGLVQSSWAVGWAGSALAFWGVYAVLPPETAWKVLFWIGVLPAL 181 Query: 184 LVVYVRRSVSETDAFLAGRKAATSKPAASTLLGIFRPDVLRLTLIGSLLGVGAHGGYYAL 243 L +Y+RR++SE +LA + L IF D+LR TL+ SLL G G YY++ Sbjct: 182 LTLYIRRAISEPAVYLATQARLRESGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYSV 241 Query: 244 MTWLPTYLKTEKHLSVLSTGGYLAVIIIAFWCGCIASAQLLDRIGRRRTVASFALCCVAT 303 TWLPT+LKTE+HLSVL T YL V+I +CG + SA L D IGRRR FA + Sbjct: 242 TTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMLFAAMGIVL 301 Query: 304 VLCYIFLPLTDTQMLICGFPLGFFAAGIPASLGALFNELYPSGIRGTGVGFCYNFGRVAS 363 VL Y + +TD ML GF LGFF +GI + +GA +EL+PS +RG+G GFCYNFGR Sbjct: 302 VLSYTQMIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYNFGRAVG 361 Query: 364 AGFPVLVGHMSDSMPLGQAIGMRAFKSLGGIDAAIAYSLVVIAVLMLPETKGRQME 419 A P LVG++S+S+PLGQ IG AA Y LV++A L+LPET+G+++E Sbjct: 362 AICPALVGYLSNSLPLGQTIGW---------IAAGCYGLVIVACLVLPETRGKELE 408 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 93.2 bits (230), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 97/335 (28%), Positives = 149/335 (44%), Gaps = 33/335 (9%) Query: 56 GATLVASAIGGWFAGALSDRYGRVKTLQITIIWFSFFTFLSAFAQSYPQLLVL------- 108 G + +G GA SDR GR L ++I+ + T + AF SY + +L Sbjct: 67 GVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIAL 126 Query: 109 -KALQGFGFGGEWAAGAVLMAETIRAEHRGRAMGTVQSAWAVGWGGAVLLFSVLF----- 162 K +QGF GGE+ + + E A HR S+W V G LL + LF Sbjct: 127 GKMIQGFSAGGEFGSSTAFLVE--HAPHRRGFF----SSWQVASQGISLLLAALFGAVLN 180 Query: 163 -TMLPQEMA---WRVMFVIGLLPALLVVYVRRSVSETDAFLAGRKAATSKPAASTLLGIF 218 + P+++A WRV F+ GLL A +Y+RR + E F +S+ + L F Sbjct: 181 NMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEF-----KESSEKTNAPLRDTF 235 Query: 219 RPDVLRLTLIGSLLGVGAHGGYYALMTWLPTYLKTEKHLSVLSTGGYLAVIIIAFWCGCI 278 +RL LIG+ + A Y L ++PTY + L S+ ++V + + G Sbjct: 236 AHQKMRL-LIGAGSVIMATVSVY-LSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSP 293 Query: 279 ASAQLLDRIGRRR-TVASFALCCVATVLCYIFLPLTD--TQMLICGFPLGFFAAGIPASL 335 L D+IGR + S AL V T ++FL + Q+L+ +G A++ Sbjct: 294 LVGALSDKIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAM 353 Query: 336 GALFNELYPSGIRGTGVGFCYNFGRVASAGFPVLV 370 AL +++P RGTG+ YN GF L+ Sbjct: 354 PALLADIFPVATRGTGMSLAYNIAVTIFGGFAGLI 388 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 83.2 bits (204), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 95/386 (24%), Positives = 158/386 (40%), Gaps = 45/386 (11%) Query: 26 DALDVQMFSLAIPALIAAFSLSKADAGMISGATLVASAIGGWFAGALSDRYGRVKTLQIT 85 DA + +P L+ + LS A G++ + + IG F G +++RYGRV + +T Sbjct: 56 DAFHALSLAFVLPVLVGLWQLSAAQIGLLIASGYLGQVIGALFFGWMAERYGRVPSASVT 115 Query: 86 IIWFSFFTFLSAFAQSYPQLLVLKALQGFGFGGEWAAGAVLMAETIRAEHRGRAMGTVQS 145 + S + + A S+ L + + LQG G GGE A + E +A RG+ + Sbjct: 116 VGLMSVLSIVCALTGSFHMLFLCRFLQGIGVGGEVPVAATYINELSQAHGRGKFFVLYEL 175 Query: 146 AWAVGWGGAVLLFSVLFTMLPQEMAWRVMFVIGLLPALLVVYVRRSVSETDAFLAGR--- 202 + +G +L + + L W MF++G +P +LV + + E+ +L R Sbjct: 176 IFPLG----LLAAAQVGAFLVPRFGWEYMFLVGGIPGILVALLITRLPESPRWLIARGRL 231 Query: 203 -------------------KAATSKPAASTLLG----------------IFRPDVLRLTL 227 A T K A + +F P T+ Sbjct: 232 DDAEKLIRTLEASTPKRNLDAQTDKQAIEQRINALAAGAGKRQRGSWKELFSPVYRGRTI 291 Query: 228 IGSLLGVGAHGGYYALMTWLPTYLKTEKHLSVLSTGGYLAVIIIAFWCGCIASAQLLDRI 287 I LL ++ + WLP+ KT HL + + +V + C + A L+DR+ Sbjct: 292 IVWLLWASSYFVANGINNWLPSLYKTVYHLPLQESLRMASVSNVLSACAVLGCALLIDRV 351 Query: 288 GRRR-TVASFALCCVATVLCYIFLPLTDTQMLICGFPLGFFAAGIPASLGALFN-ELYPS 345 GRRR +ASF L L + + ++ + G L L+ E+YP+ Sbjct: 352 GRRRWAIASFILSGALLALLGVLKAESAWSVMFLASS-AYAVMGTTTVLLYLYTPEIYPT 410 Query: 346 GIRGTGVGFCYNFGRVASAGFPVLVG 371 +R G G ++ R ASA P +VG Sbjct: 411 RMRAIGTGLATSWLRAASAAAPAMVG 436 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 82.4 bits (202), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 92/356 (25%), Positives = 153/356 (42%), Gaps = 30/356 (8%) Query: 23 WALDALDVQMFSLAIPALIAAFSLSKADAGMISGATLVASAIGGWFAGALSDRYGRVKTL 82 W DA+ V + P + F L+ A V +G + G L+DR GR L Sbjct: 32 WLADAMQVLSIGFSAPTIAKTFGLTVPQALQTGTMFFVGMLLGAFVFGRLADRIGRRPVL 91 Query: 83 QITIIWFSFFTFLSAFAQSYPQLLVLKALQGFGFGGEWAAGAVLMAETIRAEHRGRAMGT 142 ++ + F SAFA + LL L+ L G G GG +MAE + + RGR + Sbjct: 92 MGAVVIDACFGVASAFAPDFTWLLALRLLTGIGVGGTLPVDYTMMAEFLPSARRGRWLVM 151 Query: 143 VQSAWAVGWGGAVLLFSVLFTMLPQEMAWRVMFVIGLLPALLVVYVRRSVSETDAFL--A 200 ++S WA+ G L L + AWRV+F + LPAL+ V +R V E+ +L + Sbjct: 152 LESFWAI--GTICLAVLALVAVSWGNDAWRVIFFVTGLPALVGVVLRFYVPESPMYLNRS 209 Query: 201 GRKAATSK--------PAASTLLGIFRPDVLRLTLIGSLLGVGAHG-------------- 238 G+ A + +ST + +P+V+ + +L Sbjct: 210 GKSDAARQVLERVAKVNGSSTPIPPLQPEVVERKPMSALFSAALRRRSLSLYLAWGLISI 269 Query: 239 GYYALMTWLPTYLKTEKHLSVLSTGGYLAVIIIAFWCGCIASAQLLDRIGRRRTVASFAL 298 YY + +LP L +E + +L + + G SA ++R GR+ T+ F Sbjct: 270 AYYGVFVYLPVKLGSE-GFGFMRGQIFLIFLALVQLPGYALSAYGVERWGRKPTLVGF-- 326 Query: 299 CCVATVLCYIFLPLTDTQMLICGFPLGFFAAGIP-ASLGALFNELYPSGIRGTGVG 353 ++ V C ++ D +++ L F+ A+L A E+YP+ +R +G+G Sbjct: 327 LVLSAVGCMLYSLGKDPMLVVGSTLLLSFSLLGTWAALYAFTPEVYPTDLRASGMG 382 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.140 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,287,666 Number of extensions: 55378 Number of successful extensions: 382 Number of sequences better than 1.0e-08: 29 Number of HSP's gapped: 338 Number of HSP's successfully gapped: 29 Length of query: 422 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 334 Effective length of database: 1,203,190 Effective search space: 401865460 Effective search space used: 401865460 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)