BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0088.p (216 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313883|ref|YP_003777975.1| glutathione S-transferase [Herb... 278 3e-76 gi|300313568|ref|YP_003777660.1| glutathione S-transferase [Herb... 69 3e-13 gi|300309973|ref|YP_003774065.1| glutathione S-transferase [Herb... 57 8e-10 gi|300313851|ref|YP_003777943.1| glutathione S-transferase [Herb... 55 4e-09 >gi|300313883|ref|YP_003777975.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 225 Score = 278 bits (710), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 145/206 (70%), Positives = 161/206 (78%) Query: 9 ALQLHVDAQYASPYAMSVFVALREKALPFALHTVDLDAAAHHDPAYAALSLTARVPTLQH 68 LQL+VDAQ+ASPYAMSVFVALREK LPF LHT+DLD H+P YA SLT+RVP L+H Sbjct: 20 VLQLYVDAQFASPYAMSVFVALREKQLPFTLHTLDLDRGQQHEPDYAQRSLTSRVPMLRH 79 Query: 69 GELALSESSAITEYLEDVFDGAPLYPSDPAARARARQVQAWLRSDLMPIREERNTQVLFY 128 G L LSESSAITEYL++VF G LYP D ARARARQVQAWLRSDLM +REERNTQV+FY Sbjct: 80 GALTLSESSAITEYLDEVFAGPRLYPQDAVARARARQVQAWLRSDLMALREERNTQVVFY 139 Query: 129 AARKPPLSAQAEATAQRLFRFAEALLPQAADHLFGQWSIADTDLALMINRLALHGDPVPP 188 A+K LS +A A AQRLF FA LLP AD LFGQW IAD DLALM+NRLALHGD VPP Sbjct: 140 GAKKAALSEEASAAAQRLFHFAGQLLPVGADSLFGQWCIADVDLALMLNRLALHGDRVPP 199 Query: 189 RLAAYAKAQWQRSTVQEWVAMQRPPL 214 L YA+ QWQR +VQ W+ RP L Sbjct: 200 HLEDYARRQWQRPSVQAWLTQPRPAL 225 >gi|300313568|ref|YP_003777660.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 233 Score = 68.9 bits (167), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 63/195 (32%), Positives = 92/195 (47%), Gaps = 12/195 (6%) Query: 20 SPYAMSVFVALREKALPFALHTVDLDAAAHHDPA--YAALSLTARVPTLQHGELALSESS 77 S ++M +VA+ +PF + LD A D A A + + RVP L G++A+ +S Sbjct: 32 SSWSMRPWVAMVAFDIPFKEVRLLLDQA---DTAAEIARYTGSGRVPVLLDGDIAIWDSL 88 Query: 78 AITEYLEDVFDGAPLYPSDPAARARARQVQAWLRSDLMPIREERNTQVLFYAARKPPLSA 137 +I EYL + F L+P+ ARA AR + A + S +R + + R P Sbjct: 89 SICEYLAEQFPDRHLWPARTDARAIARSICAEMHSGFTGLRSAMSMDI---RGRYPGQGR 145 Query: 138 QAEATAQ--RLFRFAEALLPQAADH--LFGQWSIADTDLALMINRLALHGDPVPPRLAAY 193 AEA A R+ E L Q H LFG +SIAD A ++ R + P+ P L AY Sbjct: 146 TAEAQADIARVCEIWEECLSQFGHHDFLFGDFSIADAFYAPVVMRFISYQVPLAPALQAY 205 Query: 194 AKAQWQRSTVQEWVA 208 A + V W+A Sbjct: 206 ADRVREHPAVARWIA 220 >gi|300309973|ref|YP_003774065.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 218 Score = 57.4 bits (137), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 54/179 (30%), Positives = 84/179 (46%), Gaps = 11/179 (6%) Query: 37 FALHTVDLDAAAHHDPAYAA----LSLTARVPTLQHGELALSESSAITEYLEDVFDGAPL 92 FA + + D DP+ A LS + VP+L HG++ + ++ AI EYL ++ A L Sbjct: 26 FAGLSFEEDIVPPDDPSARAEILLLSSSILVPSLHHGDVLVWDTLAIAEYLNEIRPAAKL 85 Query: 93 YPSDPAARARARQVQAWLRSDLMPIREE--RNTQVLFYAARKPPLSAQAEATAQRLFR-F 149 P+D AARA R V + S +R N + F + + ++AEA R+ + Sbjct: 86 LPADLAARAHCRSVCGEMHSGFSALRAALPMNLKGHFPNFK---VWSRAEADIVRITTIW 142 Query: 150 AEALLPQAADHLFG-QWSIADTDLALMINRLALHGDPVPPRLAAYAKAQWQRSTVQEWV 207 E L LFG Q ++AD A ++ R + + P AYAK +QEW+ Sbjct: 143 KECLAKYGGPFLFGEQRTVADAMYAPVVTRFMTYDVKLDPICTAYAKRIMAMPEMQEWI 201 >gi|300313851|ref|YP_003777943.1| glutathione S-transferase [Herbaspirillum seropedicae SmR1] Length = 207 Score = 55.5 bits (132), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 49/154 (31%), Positives = 70/154 (45%), Gaps = 17/154 (11%) Query: 51 DPAYAALSLTARVPTLQHGELALSESSAITEYLEDVFDGAPLYPSDPAARARARQVQAWL 110 +PA+ A++ A VP ++ L ES+AI YL + GA LYP ARAR Q W Sbjct: 41 EPAFLAMNPNAMVPVIEEDGFVLWESNAIIRYLANRHGGAALYPLQAQARARVDQWIDWQ 100 Query: 111 RSDLMPIREERNTQVLFYAARKPPLSAQAEATAQRL---FRFAEAL---LPQAADHLFG- 163 SDL + RK P A+ A + RF E L L + ++ G Sbjct: 101 ASDL----NRAWSYAYLTLVRKSPAHGNAQQVAASIADWTRFMEVLEQQLQHSGGYVAGA 156 Query: 164 QWSIADTDLALMINRLALHGDPVP----PRLAAY 193 ++++AD + L +NR G P P +AAY Sbjct: 157 EFTLADISVGLSVNR--WFGTPFEHPPLPAVAAY 188 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.130 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 609,464 Number of extensions: 19955 Number of successful extensions: 81 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 79 Number of HSP's successfully gapped: 4 Length of query: 216 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 135 Effective length of database: 1,236,335 Effective search space: 166905225 Effective search space used: 166905225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)