BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0087.p (408 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313884|ref|YP_003777976.1| phthalate permease of the major... 780 0.0 gi|300313393|ref|YP_003777485.1| major facilitator superfamily p... 445 e-126 gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 163 2e-41 gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 119 3e-28 gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspir... 92 7e-20 >gi|300313884|ref|YP_003777976.1| phthalate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 418 Score = 780 bits (2013), Expect = 0.0, Method: Compositional matrix adjust. Identities = 394/408 (96%), Positives = 401/408 (98%) Query: 1 MVTLVTLALIVNYLARNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAVMQPVAGYVLD 60 MVTLVTLALIVNYLARNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAVMQPVAGY+LD Sbjct: 11 MVTLVTLALIVNYLARNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAVMQPVAGYILD 70 Query: 61 AVGTKIGFGIFALAWSLVCAAAALATGWQSLAFFRALLGMTEAAGIPGGVKASTEWFPAK 120 AVGTKIGFGIFALAWSLVCAAAA ATGWQSLAFFRALLG+TEAAGIPGGVKASTEWFPAK Sbjct: 71 AVGTKIGFGIFALAWSLVCAAAAFATGWQSLAFFRALLGITEAAGIPGGVKASTEWFPAK 130 Query: 121 ERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGVLGVLWFFLWMLFYKSPRD 180 ERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVG LGV+WF LWMLFYKSPRD Sbjct: 131 ERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGALGVIWFVLWMLFYKSPRD 190 Query: 181 QKLLSPEERDYILDGQEKTPEKVVRESWTKIVRSQNFWSIAIPRFLSEPAWQTFNAWIPL 240 QKLLSPEER YIL+GQEK+PEKV RESWTKIVRS+NFWSIAIPRFLSEPAWQTFNAWIPL Sbjct: 191 QKLLSPEERAYILEGQEKSPEKVQRESWTKIVRSRNFWSIAIPRFLSEPAWQTFNAWIPL 250 Query: 241 YMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPFFHKHLNVSLFTSRKLVMLVGSLA 300 YMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSP FHKHL VSLFTSRKLVMLVGSL+ Sbjct: 251 YMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPLFHKHLKVSLFTSRKLVMLVGSLS 310 Query: 301 MIGPACVGFVDSPYVAIALLSIGGFAHQTLSGALYSITSDVFTKNQVATATGLTGMSGYL 360 MIGPACVGFVDSPYVAIALLSIGGFAHQTLSGALYSITSDVFTKNQVATATGLTGMSGYL Sbjct: 311 MIGPACVGFVDSPYVAIALLSIGGFAHQTLSGALYSITSDVFTKNQVATATGLTGMSGYL 370 Query: 361 GATLFTLLFGILVTQIGYGPLFVLLAAFDLVAVAVVFAIARPRPEAIA 408 GATLFTLLFGILVTQIGYGPLFVLLAAFDLVAVAVVFAIARPRPEAIA Sbjct: 371 GATLFTLLFGILVTQIGYGPLFVLLAAFDLVAVAVVFAIARPRPEAIA 418 >gi|300313393|ref|YP_003777485.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 445 bits (1144), Expect = e-126, Method: Compositional matrix adjust. Identities = 216/403 (53%), Positives = 286/403 (70%), Gaps = 3/403 (0%) Query: 1 MVTLVTLALIVNYLARNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAVMQPVAGYVLD 60 ++ LV L I NYLARN+L V APT+ EL MSTQQYSY+V A+Q+ Y +MQPV G+++D Sbjct: 11 IIVLVCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGYTIMQPVCGFIVD 70 Query: 61 AVGTKIGFGIFALAWSLVCAAAALATGWQSLAFFRALLGMTEAAGIPGGVKASTEWFPAK 120 +G +IGF +F + WS+ A ATGW SLA FRAL+G+TEAA IP G+KA EWFP K Sbjct: 71 LIGLRIGFALFGVLWSVAGVLHAGATGWLSLAGFRALMGLTEAAAIPSGMKAVAEWFPDK 130 Query: 121 ERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGVLGVLWFFLWMLFYKSPRD 180 E+SVA+GWFN G+S+GA+ APPLV++ + GW+ +FVV G+LG +W LW FY+SPRD Sbjct: 131 EKSVAVGWFNSGTSLGAMLAPPLVIFLQIRYGWQSAFVVTGILGFVWAALWFAFYRSPRD 190 Query: 181 QKLLSPEERDYILDGQEKTPEKVVR--ESWTKIVRSQNFWSIAIPRFLSEPAWQTFNAWI 238 LS ER+ I+ GQ + P VR +VRS+ FW+IA+ RF +EPAWQTF+ WI Sbjct: 191 HAALSDSERETIISGQLR-PVSAVRGKRPVKDVVRSRRFWAIALARFFAEPAWQTFSFWI 249 Query: 239 PLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPFFHKHLNVSLFTSRKLVMLVGS 298 PLY ERH+++K IAMFAWLPFLAAD+G + GGYLSPF + L SR +++G+ Sbjct: 250 PLYFVNERHLDLKSIAMFAWLPFLAADLGGLAGGYLSPFLINRFRIPLVWSRVCGVVLGA 309 Query: 299 LAMIGPACVGFVDSPYVAIALLSIGGFAHQTLSGALYSITSDVFTKNQVATATGLTGMSG 358 + MIGPACVG V SPYVAIAL +GGFAHQ +SG + ++++DVF +V TA+G GMS Sbjct: 310 VMMIGPACVGLVASPYVAIALFCVGGFAHQMISGLVNTLSADVFDPEEVGTASGFAGMSA 369 Query: 359 YLGATLFTLLFGILVTQIGYGPLFVLLAAFDLVAVAVVFAIAR 401 ++G F+LL G L +GYGPLF +L AFDL+ ++ + R Sbjct: 370 WIGGLGFSLLVGALADTVGYGPLFGMLGAFDLIGAVLLIILIR 412 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 163 bits (412), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 111/380 (29%), Positives = 183/380 (48%), Gaps = 10/380 (2%) Query: 2 VTLVTLALIVNYLARNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAVMQPVAGYVLDA 61 ++ + LA IVNYL R+TLS+A ++ +EL++S Q ++ A+ + YA Q G +LD Sbjct: 19 LSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFSLAYAFSQLPIGAMLDK 78 Query: 62 VGTKIGFGIFALAWSLVCAAAALATGWQSLAFFRALLGMTEAAGIPGGVKASTEWFPAKE 121 G ++ G WS+ A Q R LG+ EA P G K +EWF +E Sbjct: 79 FGARVMLGAGMFIWSVAQLAGGFVNNLQQFLVARVFLGLGEAPQFPAGAKVVSEWFAMRE 138 Query: 122 RSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGVLGVLWFFLWMLFYKSPRDQ 181 R G F S+IG APP++ +L GW+M FV++GVLG+ W LFY++ R++ Sbjct: 139 RGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGVLGIAVSVGWYLFYRN-RNE 197 Query: 182 KLLSPEERDYILDGQEKTPEKVVRESWTK---IVRSQNFWSIAIPRFLSEPAWQTFNAWI 238 L+P E Y+ D + P + SW++ + + W + + W+ Sbjct: 198 VQLTPSEVLYLADDHDNDPNANAKLSWSEWKSLFTYRTTWGMLFGFMGVIYMVWLYLTWL 257 Query: 239 PLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPFFHKHLNVSLFTSRK---LVML 295 P Y+ ERH++I +P++ +G + GY++ K + SRK L Sbjct: 258 PAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR-GMPAIRSRKWPICAGL 316 Query: 296 VGSLAMIGPACVGFVDSPYVAIALLSIGGFAHQTLSGALYSITSDVFTKNQVATATGLTG 355 +G+ A PA + S +AI +S+ SG +++ S + VA+ + Sbjct: 317 IGAGAFTIPAA--YTPSTTMAIVYISLAMAFVNMASGGAWALVSVAIPRRLVASLGSMQN 374 Query: 356 MSGYLGATLFTLLFGILVTQ 375 GYLG +L ++ G++V Q Sbjct: 375 FGGYLGGSLAPVITGVVVDQ 394 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 119 bits (299), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 100/387 (25%), Positives = 170/387 (43%), Gaps = 27/387 (6%) Query: 11 VNYLARNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAVMQPVAGYVLDAVGTKIGFGI 70 VNY R TLS+A M EL + YI A+ Y + Q G++LD G+K + Sbjct: 25 VNYADRATLSIAGSAMKTELSLDPVMMGYIFSAFSWAYVLAQLPGGWLLDRFGSKKIYMW 84 Query: 71 FALAWSL----------VCAAAALATGWQSLAFFRALLGMTEAAGIPGGVKASTEWFPAK 120 WS+ + AAA+A SL R ++G+ EA P + WFP K Sbjct: 85 SIALWSVFTFLQAGVTWMSTAAAVA----SLFLLRFMVGLAEAPSFPANGRIVAAWFPTK 140 Query: 121 ERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGVLGVLWFFLWMLFYKSPRD 180 ER A FN A+ PL+ W + W F V+GV+G+ +W SP+D Sbjct: 141 ERGTASAIFNSAQYFAAVLFTPLMAWIVHTYSWHHVFTVMGVVGIALALIWGRIIYSPKD 200 Query: 181 QKLLSPEERDYILDG---------QEKTPEKVVRESWTKIVRSQNFWSIAIPRFLSEPAW 231 ++ E +I G + P R +++ ++ + I ++ Sbjct: 201 HPRINQAELAHIESGGGLVDMDNRKVAAPSTKGRGYIRQLLSNRMLLGVYIGQYCINVIT 260 Query: 232 QTFNAWIPLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPFFHKHLNVSLFTSRK 291 F W P+Y+ ER M+I + A +P + IG V+GG +S K +S+ +RK Sbjct: 261 YFFLTWFPVYLVQERGMSILKAGFVASIPAVCGFIGGVVGGLISDRLIKR-GLSVTWARK 319 Query: 292 LVMLVGSLAMIGPACVGFVDSPYVAIALLSIGGFAHQTLSGAL-YSITSDVFTKNQVATA 350 + ++ G L + +V++ ++ + ++++ F GAL +++ +D K V + Sbjct: 320 IPIVGGMLLSMTMILCNYVEAQWMVVGIMALAFFGKGV--GALGWAVVADTAPKQIVGLS 377 Query: 351 TGLTGMSGYLGATLFTLLFGILVTQIG 377 GL M G + ++ G +V + G Sbjct: 378 GGLFNMFGNVAGITTPIVIGYIVKETG 404 >gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 92.0 bits (227), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 79/315 (25%), Positives = 138/315 (43%), Gaps = 31/315 (9%) Query: 16 RNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAVMQPVAGYVLDAVGTKIGFGIFALAW 75 R L VA P + KE MS + + + Y++ Q +G G F + LA Sbjct: 34 RANLGVALPFLRKEFVMSNTEAGALASIFLFAYSLAQLPSGLAYTRFGIPRVFSLAMLAT 93 Query: 76 SLVCAAAALATGWQSLAFFRALLGMTEAAGIPGGVKAS-TEWFPAKERSVAIGWFNIGSS 134 S+ A+ +L FR LG+ E +P G+ ++ WFPA+E+ G F + Sbjct: 94 SVFTGLIGTASSLLALKIFRFGLGLAEGP-LPIGITSTINNWFPAREKGTMTGIFLAAAK 152 Query: 135 IGALCAPPLVVWTILHGGWKMSFVVVGVLGVLWFFLWMLFYKS-PRDQKLLSPEERDYIL 193 G + PP+ + + GW+ F V GV+ +W++ K+ P + +S EE ++I Sbjct: 153 FGPVLVPPVCAVIMYYWGWRHIFYFFAVPGVILSVVWLMVVKNRPSESPHVSAEELEHIN 212 Query: 194 D------GQEKTP---------EKVVRESWTKIVRSQ-------NFWSIAIPRFLSEPAW 231 D GQ+ P ++++R S + + N A+ F Sbjct: 213 DSQGAAAGQDADPAVTRDFALLDRIIRRSKVATIETTKGVFCSWNIMGAALGYFFMTAIV 272 Query: 232 QTFNAWIPLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPFFHKH------LNVS 285 AWIP Y+ + + +I + M A P++ A IG +LGG ++ + + + + Sbjct: 273 NVLLAWIPTYLISVKGYSIANMGMVAAAPWVGAVIGNLLGGAMTDRWLEKRRKPAMMMSA 332 Query: 286 LFTSRKLVMLVGSLA 300 FT+ +V+L+ S A Sbjct: 333 FFTTIMMVILINSPA 347 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.138 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,151,672 Number of extensions: 42323 Number of successful extensions: 246 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 229 Number of HSP's successfully gapped: 21 Length of query: 408 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 321 Effective length of database: 1,207,925 Effective search space: 387743925 Effective search space used: 387743925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)