BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0078.p (500 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 824 0.0 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 498 e-142 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 396 e-111 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 330 2e-91 gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipo... 214 2e-56 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 824 bits (2128), Expect = 0.0, Method: Compositional matrix adjust. Identities = 427/492 (86%), Positives = 454/492 (92%), Gaps = 1/492 (0%) Query: 1 MTRPAQQTSILWTRTAMVLALTAAASWTQQVDGKPLGXLAPVPASWTQQVDGKPLGVSAG 60 M +P Q+ S LW RTAM+LALTAA + + APVPASWT Q +P SAG Sbjct: 1 MMQPVQRKSTLWPRTAMLLALTAALAGCANLAPDYTQPAAPVPASWTSQAAAQPAVGSAG 60 Query: 61 KAAGEIDADQLGWREFFLDPRLQQVIAAALENNRDLRVAALNIEKARAQYRITDADRYPA 120 AAGEIDAD LGWREFFLDPRLQQVIAAAL+NNRDLRVAALNIEKARAQYRITDA+RYP+ Sbjct: 61 PAAGEIDADHLGWREFFLDPRLQQVIAAALDNNRDLRVAALNIEKARAQYRITDANRYPS 120 Query: 121 ISATGTSTASRTPSELTTSGRATVSRQQSVTVGISAYELDFFGRLKNLSDAALETYQYQV 180 ISATGTS+ASRTP+EL+T+G A VS QQSVTVG+SAYELDFFGRLKNLSDAALE+YQYQV Sbjct: 121 ISATGTSSASRTPAELSTTGSAAVSHQQSVTVGVSAYELDFFGRLKNLSDAALESYQYQV 180 Query: 181 ETRRSTHISLVAEVATAWLTLASDRELLKLAQDTYASQSRTFELSQRSHDIGTVSGVDLA 240 ETRRSTHISLVAEVATAWLTLASDR+LLKLAQDTYASQSRTF LSQRSHDIGTVSGVDLA Sbjct: 181 ETRRSTHISLVAEVATAWLTLASDRDLLKLAQDTYASQSRTFALSQRSHDIGTVSGVDLA 240 Query: 241 SQEATVQAARADVASYTSQVAQDINALRLLVGADIDAALLPTGLPDTASVLPATPAGLPS 300 SQEATV++ARADVA Y SQV QD+NALRLLVGADIDAALLP GLPDTASVLPATPAGLPS Sbjct: 241 SQEATVESARADVARYASQVEQDLNALRLLVGADIDAALLPAGLPDTASVLPATPAGLPS 300 Query: 301 QVLLRRPDVLAAEHTLKSANADIGAARAAFFPSISLTADGGTASRTLGGLFKAGSGSWSF 360 QVLLRRPDVLAAEHTLKSANADIGAARAAFFPSISLTA+GGTASRTLGGLFKAGSGSW+F Sbjct: 301 QVLLRRPDVLAAEHTLKSANADIGAARAAFFPSISLTANGGTASRTLGGLFKAGSGSWTF 360 Query: 361 VPSINLPIFNAGSLRASLDSAKITRDIDVANYEKAIQTAFREVADALAVRATLDQRLDAQ 420 PSINLPIFNAGSLRASLDSAKITRDIDVANYEKAIQTAFREVADALAVRATLDQRLDAQ Sbjct: 361 SPSINLPIFNAGSLRASLDSAKITRDIDVANYEKAIQTAFREVADALAVRATLDQRLDAQ 420 Query: 421 GRATAASEKAFRLSEARYKNGIDSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVL 480 G+ATAASEKAFRLS+ARY+NG+DSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVL Sbjct: 421 GKATAASEKAFRLSDARYRNGVDSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVL 480 Query: 481 GGGWNEDTAQPV 492 GGGW+E T QP+ Sbjct: 481 GGGWSEQT-QPM 491 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 498 bits (1281), Expect = e-142, Method: Compositional matrix adjust. Identities = 259/441 (58%), Positives = 330/441 (74%), Gaps = 5/441 (1%) Query: 58 SAGKAAGEIDADQLGWREFFLDPRLQQVIAAALENNRDLRVAALNIEKARAQYRITDADR 117 +AG ++ + AD +GWR+ F DP LQQVIA ALENNRDLRVA LNIEKARAQY + A Sbjct: 52 AAGASSAPVVAD-IGWRDVFTDPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAAL 110 Query: 118 YPAISATGTSTASRTPSELTTSGRATVSRQQSVTVGISAYELDFFGRLKNLSDAALETYQ 177 +P+I A + TASRTP +L+++G A SR + T+G SAYELD FGR+++L + AL+++ Sbjct: 111 FPSIKAATSETASRTPGDLSSTGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFL 170 Query: 178 YQVETRRSTHISLVAEVATAWLTLASDRELLKLAQDTYASQSRTFELSQRSHDIGTVSGV 237 E RRST ISL+AEVATAWLTLASD++ L+LAQDT SQS ++ L+QRS ++G+ S + Sbjct: 171 STAEARRSTQISLIAEVATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSAL 230 Query: 238 DLASQEATVQAARADVASYTSQVAQDINALRLLVGADIDAALLPTGLPDT----ASVLPA 293 L + +V++AR DV SYT+QV QD NAL LLVG D+ ALLP GLPDT AS L Sbjct: 231 TLRQAQTSVESARVDVESYTAQVDQDRNALVLLVGKDLPTALLPQGLPDTRLAAASPLAT 290 Query: 294 TPAGLPSQVLLRRPDVLAAEHTLKSANADIGAARAAFFPSISLTADGGTASRTLGGLFKA 353 P GLPS +L RR D++ AE L++ANA+IGAARAAF+PSI+LT + GTAS L GLFK Sbjct: 291 IPPGLPSDLLQRRADIVQAERDLRAANANIGAARAAFYPSITLTVNAGTASARLAGLFKG 350 Query: 354 GSGSWSFVPSINLPIFNAGSLRASLDSAKITRDIDVANYEKAIQTAFREVADALAVRATL 413 GSGSWSFVP I+LPIF+ G+ +A+LD A ++RDI VA YEKAIQTAFREV+DALA R TL Sbjct: 351 GSGSWSFVPQISLPIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTL 410 Query: 414 DQRLDAQGRATAASEKAFRLSEARYKNGIDSYLTTLVAQRTLYSAQQTLISLRLTEQSNR 473 ++L AQ AS++AFRLS+ARY +G+DSYLT L +QRTLYSAQ+ LI +L+ +N Sbjct: 411 GRQLQAQEALVQASDEAFRLSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNL 470 Query: 474 ITLYKVLGGGWNEDTAQPVAA 494 +T YK LGGGW E T P A+ Sbjct: 471 VTFYKTLGGGWVERTGTPAAS 491 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 396 bits (1018), Expect = e-111, Method: Compositional matrix adjust. Identities = 224/457 (49%), Positives = 296/457 (64%), Gaps = 12/457 (2%) Query: 40 APVPASWTQQVDGKPLGVSAGKAAGEIDADQLGWREFFLDPRLQQVIAAALENNRDLRVA 99 APV +S+ G+ E+ A LGWR+FF D RLQQ+IAAALENNRDLR A Sbjct: 39 APVESSYPADAAGR----------AELQAVNLGWRQFFPDQRLQQLIAAALENNRDLRTA 88 Query: 100 ALNIEKARAQYRITDADRYPAISATGTSTASRTPSELTTSGRATVSRQQSVTVGISAYEL 159 LNIE+ARAQY+I AD P ++ +G + +RT + + +G+ T+S V + + AYEL Sbjct: 89 VLNIEEARAQYQIARADLLPNLNLSGNAARTRTAASQSLTGQPTISNSYQVGLAVPAYEL 148 Query: 160 DFFGRLKNLSDAALETYQYQVETRRSTHISLVAEVATAWLTLASDRELLKLAQDTYASQS 219 DFFGR++NL DAAL +Y E ++S ISLVA+VA A+L S E LKLA +T S+ Sbjct: 149 DFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQAYLAERSYAEQLKLADETLKSRQ 208 Query: 220 RTFELSQRSHDIGTVSGVDLASQEATVQAARADVASYTSQVAQDINALRLLVGADIDAAL 279 +L+Q+ D+G + +D + V++AR Q AQ NAL LLVG I L Sbjct: 209 SNLDLAQKRLDVGASTALDFRLTQTLVESARVAKTQLERQRAQAENALTLLVGKKI-GEL 267 Query: 280 LPTGLPDTASVLPATPAGLPSQVLLRRPDVLAAEHTLKSANADIGAARAAFFPSISLTAD 339 P L ++ PAGLPS +L RPD+ +AE +L +ANA+IGAARAAFFP ISLT + Sbjct: 268 PPAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSAEQSLLAANANIGAARAAFFPRISLTGN 327 Query: 340 GGTASRTLGGLFKAGSGSWSFVPSINLPIFNAGSLRASLDSAKITRDIDVANYEKAIQTA 399 G+AS TLGGLF++GSG+WSF PS++LPIF+ G A+LD A++ ++ V YEKAIQTA Sbjct: 328 FGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRNSANLDLAEVRKNKAVVTYEKAIQTA 387 Query: 400 FREVADALAVRATLDQRLDAQGRATAASEKAFRLSEARYKNGIDSYLTTLVAQRTLYSAQ 459 FREVADAL R +LD+++ AQ AA ++ +L++ R+KNGI S L L AQR L+SAQ Sbjct: 388 FREVADALVARGSLDEQVKAQEAFLAAQQERLKLTDLRFKNGIASSLDQLDAQRDLFSAQ 447 Query: 460 QTLISLRLTEQSNRITLYKVLGGGWNEDTAQPVAAVQ 496 Q LI R +N I LYK LGGG NE+T VAA Q Sbjct: 448 QALIQARQLRLNNAIDLYKALGGGLNENTVS-VAAKQ 483 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 330 bits (846), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 207/446 (46%), Positives = 275/446 (61%), Gaps = 11/446 (2%) Query: 40 APVPASWTQQVDGKPLGVSAGKAAGEIDADQLGWREFFLDPRLQQVIAAALENNRDLRVA 99 APV A + DG P+ +A + A E + L WR++F DP LQ +I ALEN+RDLR A Sbjct: 37 APVAAVYP---DG-PVNEAAHQVANEAASPPLPWRDYFTDPPLQALITQALENSRDLRTA 92 Query: 100 ALNIEKARAQYRITDADRYPAISATGTSTASRTPSELTTSGRATVSRQQSVTVGISAYEL 159 AL +++A+A Y I AD +P I A SRTP++L + R V +G+S++E+ Sbjct: 93 ALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQAGLGLSSWEI 152 Query: 160 DFFGRLKNLSDAALETYQYQVETRRSTHISLVAEVATAWLTLASDRELLKLAQDTYASQS 219 D +GRL++L DAALE+ + TR + +SL+A+VA AWL LA E L++A T AS+ Sbjct: 153 DLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDERLEIANQTIASRQ 212 Query: 220 RTFELSQRSHDIGTVSGVDLASQEATVQAARADVASYTSQVAQDINALRLLVGADIDAAL 279 +F + R ++G S ++L E + A+A A Q AQ +NAL LLVG+ + + Sbjct: 213 ESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTLLVGSPV---V 269 Query: 280 LPTGLPDT---ASVLPATPAGLPSQVLLRRPDVLAAEHTLKSANADIGAARAAFFPSISL 336 LP G A P P GLP+ +L RRPDV AAEH L +ANA+IGAARAAFFPSISL Sbjct: 270 LPVGQARAGLDAVFAPLQP-GLPALLLTRRPDVAAAEHRLMAANANIGAARAAFFPSISL 328 Query: 337 TADGGTASRTLGGLFKAGSGSWSFVPSINLPIFNAGSLRASLDSAKITRDIDVANYEKAI 396 T + GTAS L GLF+ GS +W+F PSI LPIF AG LR +L +++ RD+ VA YEK + Sbjct: 329 TGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDLAVAAYEKTV 388 Query: 397 QTAFREVADALAVRATLDQRLDAQGRATAASEKAFRLSEARYKNGIDSYLTTLVAQRTLY 456 QTAFREVADALA R L Q+L+ A + RLS RY NG +L L AQR L Sbjct: 389 QTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLDVLDAQRDLL 448 Query: 457 SAQQTLISLRLTEQSNRITLYKVLGG 482 SAQQ L+ +R S+ + LY LGG Sbjct: 449 SAQQQLVQIRRALLSSSVALYAALGG 474 >gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 481 Score = 214 bits (544), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 152/418 (36%), Positives = 223/418 (53%), Gaps = 5/418 (1%) Query: 68 ADQLGWREFFLDPRLQQVIAAALENNRDLRVAALNIEKARAQYRITDADRYPAISATGTS 127 A +GW+++F LQ +IA AL +N DLR L +++ +AQ+RI + P ++ + Sbjct: 54 ASAIGWQDYFPQAPLQALIAEALRHNPDLRDIRLRLQQVQAQWRIQRSTGLPTVNLEAQA 113 Query: 128 TASRTPSELTTSGRATVSRQQSVTVGISAYELDFFGRLKNLSDAALETYQYQVETRRSTH 187 SR P E SG A +R+ V +G S++ELDF+GR+ +L++AAL+ + ++ Sbjct: 114 RRSRGPLEPPESGPAASTREYRVGLGASSWELDFWGRIASLNEAALQEWMASRAAEQALV 173 Query: 188 ISLVAEVATAWLTLASDRELLKLAQDTYASQSRTFELSQRSHDIGTVSGVDLASQEATVQ 247 ISL+ +VA AW +L E L L + A+ + + L Q HD G LA Q + + Sbjct: 174 ISLIGQVAAAWTSLRELDERLHLLETLQAATAESLALMQARHDAGRADAAALAQQRSELA 233 Query: 248 AARADVASYTSQVAQDINALRLLVG-ADIDAALLPTGLPDTASVLP-ATPAGLPSQVLLR 305 RA+ A A + L +L G + AAL P T +LP A GLPS +L Sbjct: 234 QMRAEQAELQQMRASQRSTLEMLAGLTQLPAALEPA---PTTILLPFALAPGLPSTLLAA 290 Query: 306 RPDVLAAEHTLKSANADIGAARAAFFPSISLTADGGTASRTLGGLFKAGSGSWSFVPSIN 365 RPD++AAEH L+++ A + AARAAFFP ISLTA GG AS L LFK S +W+FVP I+ Sbjct: 291 RPDIIAAEHRLQASAAQVAAARAAFFPRISLTAFGGVASTALQDLFKGPSRAWNFVPEIS 350 Query: 366 LPIFNAGSLRASLDSAKITRDIDVANYEKAIQTAFREVADALAVRATLDQRLDAQGRATA 425 LP+ + G ++ D A + + + +YE+ +Q AFREVA L L ++L + Sbjct: 351 LPLLDGGLRQSRYDIAALDQQRALLDYERTVQNAFREVAQRLEEAHWLKEQLRLRREVVQ 410 Query: 426 ASEKAFRLSEARYKNGIDSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGGG 483 + R + G S L L ++R AQQ L N+I LY LGGG Sbjct: 411 QQAQRQAQMHMRLRYGRASRLDVLESERDHLRAQQRQHMLEHALLRNQIALYVALGGG 468 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.127 0.350 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,473,077 Number of extensions: 61563 Number of successful extensions: 362 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 313 Number of HSP's successfully gapped: 17 Length of query: 500 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 411 Effective length of database: 1,198,455 Effective search space: 492565005 Effective search space used: 492565005 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)