BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0077.p (580 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump pr... 1132 0.0 gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump pr... 664 0.0 gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 635 0.0 gi|300313244|ref|YP_003777336.1| multidrug efflux system transme... 514 e-147 gi|300309657|ref|YP_003773749.1| cation/multidrug efflux pump pr... 330 1e-91 >gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 1132 bits (2927), Expect = 0.0, Method: Compositional matrix adjust. Identities = 554/572 (96%), Positives = 567/572 (99%) Query: 1 VVVENVERLMSEEKLSPKEATRKSMQEITGALVGIAMVLSAVFIPMAFFGGSTGVIYRQF 60 VVVENVERLMSEEKLSPKEATRKSMQEITGALVGIAMVLSAVFIPMAFFGGSTGVIYRQF Sbjct: 413 VVVENVERLMSEEKLSPKEATRKSMQEITGALVGIAMVLSAVFIPMAFFGGSTGVIYRQF 472 Query: 61 SITIVSAMALSVMVALTLTPALCATLLKPHASGHVQQGKFFTWFNRTFDRSATRYQGGVG 120 SITIVSAMALSVMVALTLTPALCATLLKPHA GHVQQG+FFTWFNR+FDRSATRYQGGV Sbjct: 473 SITIVSAMALSVMVALTLTPALCATLLKPHAGGHVQQGRFFTWFNRSFDRSATRYQGGVA 532 Query: 121 GLLKRGKRGLLMYALIAVAMAVLFMRLPTSFLPEEDQGVLMAQIQLPTGATAEQTATVVK 180 GLLKRGKRGLLMYALIAVAMAVLFMRLPTSFLPEEDQGVLMAQIQLPTGATAEQTATVVK Sbjct: 533 GLLKRGKRGLLMYALIAVAMAVLFMRLPTSFLPEEDQGVLMAQIQLPTGATAEQTATVVK 592 Query: 181 TVQEYFMNKPESVDSVLAIVGAGVGGNSQNGARAFIRLKEWDQRTGAGQSASELVRLANA 240 TVQEYFMNKPESV+SVLAIVGAGVGGNSQNGARAFIRLK+W+QRTGAGQSASELVR ANA Sbjct: 593 TVQEYFMNKPESVESVLAIVGAGVGGNSQNGARAFIRLKDWEQRTGAGQSASELVRRANA 652 Query: 241 DLSKIRGARVFLMNPPAVRGLGQSSGFDLELKDVGGVGHDALLKARDQFLQLARGNALLA 300 DLSKIRGARVFLMNPPAVRGLGQSSGFDLELKDVGGVGHDALL+ARDQFLQLAR NALLA Sbjct: 653 DLSKIRGARVFLMNPPAVRGLGQSSGFDLELKDVGGVGHDALLQARDQFLQLARSNALLA 712 Query: 301 NVRTTGLDDTPQLRVAIDDRKADALSIATSDINSTLATAMGGTYVNDFLSNGRVKKVYVQ 360 NVRTTGLDDTPQLRVAIDDRKADALSIATSDIN+TLATAMGGTYVNDFLSNGRVKKVYVQ Sbjct: 713 NVRTTGLDDTPQLRVAIDDRKADALSIATSDINATLATAMGGTYVNDFLSNGRVKKVYVQ 772 Query: 361 GDSAFRMQAEDLNRWYVRNSASQMVPFSAFMSSKWTYGPSLLERYNGVSSFEIVGEPAAG 420 GDSAFRMQA+DLNRWYVRN+ASQMVPF+AFMSS+WTYGPSLLERYNGVSSFEIVGEPAAG Sbjct: 773 GDSAFRMQADDLNRWYVRNAASQMVPFAAFMSSQWTYGPSLLERYNGVSSFEIVGEPAAG 832 Query: 421 VSSGTAMAEVEKLVAQLPAGIGFEWTGSSYQERLSGNQAPMLYAISILFVFLCLAALYES 480 VSSGTAMAEVEKLVAQLPAGIGFEWTGSSYQERLSGNQAPMLYAISILFVFLCLAALYES Sbjct: 833 VSSGTAMAEVEKLVAQLPAGIGFEWTGSSYQERLSGNQAPMLYAISILFVFLCLAALYES 892 Query: 481 WSVPFAVILAVPLGIVGTVLLTGIFGMSNDVYFQVGLLTTVGLSAKNAILIVEFAKQLHE 540 WSVPFAVILAVPLGI+GTVLLTGIFGMSNDVYFQVGLLTTVGLSAKNAILIVEFAKQLHE Sbjct: 893 WSVPFAVILAVPLGIIGTVLLTGIFGMSNDVYFQVGLLTTVGLSAKNAILIVEFAKQLHE 952 Query: 541 SGKSLRDATLEAVQLRLRPILMTSLAFMFGVL 572 SGKSL +ATLEAVQLRLRPILMTSLAFMFGVL Sbjct: 953 SGKSLWEATLEAVQLRLRPILMTSLAFMFGVL 984 >gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1049 Score = 664 bits (1712), Expect = 0.0, Method: Compositional matrix adjust. Identities = 340/575 (59%), Positives = 422/575 (73%), Gaps = 3/575 (0%) Query: 1 VVVENVERLMSEEKLSPKEATRKSMQEITGALVGIAMVLSAVFIPMAFFGGSTGVIYRQF 60 VVVENVER+MSEE L PK+ATRKSM +I+GALVG+A+VL+AVF+PMAFF GSTGVIYRQF Sbjct: 411 VVVENVERVMSEEGLPPKQATRKSMGQISGALVGVALVLAAVFVPMAFFSGSTGVIYRQF 470 Query: 61 SITIVSAMALSVMVALTLTPALCATLLKPHASGH-VQQGKFFTWFNRTFDRSATRYQGGV 119 SITIVSAM LSV+VAL LTPALCATLLKP A GH + FF WFNR FDRS RYQG V Sbjct: 471 SITIVSAMTLSVLVALVLTPALCATLLKPAAKGHALATTGFFGWFNRQFDRSNIRYQGIV 530 Query: 120 GGLLKRGKRGLLMYALIAVAMAVLFMRLPTSFLPEEDQGVLMAQIQLPTGATAEQTATVV 179 +L RG R + YA++ + FM+LP FLP+EDQG L A +QLP+GAT +T V+ Sbjct: 531 RHMLGRGWRYMAAYAVLLALVVAGFMKLPVGFLPDEDQGTLFAIMQLPSGATKARTDEVI 590 Query: 180 KTVQEYFM-NKPESVDSVLAIVGAGVGGNSQNGARAFIRLKEWDQRTGAGQSASELVRLA 238 + V+++F+ ++ E+V V A+ G G+ QN AFI+LK WD+R G G S +++ A Sbjct: 591 RQVEQHFLVDQKEAVSGVFAVSGFSFAGSGQNMGFAFIKLKPWDERKGPGLSVTDVAAKA 650 Query: 239 NADLSKIRGARVFLMNPPAVRGLGQSSGFDLELKDVGGVGHDALLKARDQFLQLARGNAL 298 A + IR ARVF PPAV LG ++GFDL LKD +GH AL++AR+Q L + Sbjct: 651 GAAFANIRDARVFAFAPPAVSELGNATGFDLMLKDEANLGHAALMQARNQLLGELMQDKR 710 Query: 299 LANVRTTGLDDTPQLRVAIDDRKADALSIATSDINSTLATAMGGTYVNDFLSNGRVKKVY 358 L VR GL+DTP+ R+++D KA AL +A SDIN T+ATA G +YVNDF+ GRVKKV Sbjct: 711 LVAVRPNGLEDTPEFRLSVDTDKAGALGVAMSDINDTIATAWGSSYVNDFIDKGRVKKVM 770 Query: 359 VQGDSAFRMQAEDLNRWYVRNSASQMVPFSAFMSSKWTYGPSLLERYNGVSSFEIVGEPA 418 +QGD+ +RM D++RWYVRNSA MVPFSAF ++ WT G LERYNGV S EI+G Sbjct: 771 LQGDAQYRMLPSDVDRWYVRNSAGTMVPFSAFSTASWTSGSPRLERYNGVPSMEILGMAL 830 Query: 419 AG-VSSGTAMAEVEKLVAQLPAGIGFEWTGSSYQERLSGNQAPMLYAISILFVFLCLAAL 477 G SSG AMA VE +A++P GIG+EWTG S QE+ S + +LY +SIL VFL LAAL Sbjct: 831 PGAASSGEAMAIVEAALAKMPPGIGYEWTGLSLQEKASSGKTGILYGVSILIVFLALAAL 890 Query: 478 YESWSVPFAVILAVPLGIVGTVLLTGIFGMSNDVYFQVGLLTTVGLSAKNAILIVEFAKQ 537 YESW+VPF+VIL VPLG+ G +L + NDVYFQVGLLTT+GL+ KNAILIVEFAK Sbjct: 891 YESWAVPFSVILVVPLGVFGALLGAMLTWKMNDVYFQVGLLTTIGLACKNAILIVEFAKD 950 Query: 538 LHESGKSLRDATLEAVQLRLRPILMTSLAFMFGVL 572 L+E GKSL +AT+EAV++RLRPILMTSLAF+ GVL Sbjct: 951 LYEHGKSLVEATMEAVRMRLRPILMTSLAFILGVL 985 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 635 bits (1637), Expect = 0.0, Method: Compositional matrix adjust. Identities = 313/573 (54%), Positives = 413/573 (72%), Gaps = 3/573 (0%) Query: 1 VVVENVERLMSEEKLSPKEATRKSMQEITGALVGIAMVLSAVFIPMAFFGGSTGVIYRQF 60 VVVENVER+MSEE LSP +ATRK+M +I+GAL+GI +VL AVFIPMAFFGG+ G IYRQF Sbjct: 411 VVVENVERIMSEEGLSPADATRKAMGQISGALIGITLVLIAVFIPMAFFGGAVGAIYRQF 470 Query: 61 SITIVSAMALSVMVALTLTPALCATLLKPHASGHVQQGK-FFTWFNRTFDRSATRYQGGV 119 S+++V++M SV++A TLTPALCAT+LKP GH + K FF WFNR F+ +A+ YQG + Sbjct: 471 SLSMVASMVFSVLMAFTLTPALCATILKPVEKGHHHEKKGFFGWFNRKFNSTASGYQGII 530 Query: 120 GGLLKRGKRGLLMYALIAVAMAVLFMRLPTSFLPEEDQGVLMAQIQLPTGATAEQTATVV 179 GG+L + R +++Y LI + +L++RLP SFLP EDQG ++ +QLP GA+ +T VV Sbjct: 531 GGMLNKAGRYMIVYVLIVACVGLLYVRLPASFLPNEDQGYIITNVQLPPGASTSRTTAVV 590 Query: 180 KTVQEYFMNKPESVDSVLAIVGAGVGGNSQNGARAFIRLKEWDQRTGAGQSASELVRLAN 239 K ++EY++ P +V+ +A++G GN QN A F LK+W QR + QSA + A Sbjct: 591 KQIEEYYLKDP-AVEHAIAVLGYSFSGNGQNAALVFTPLKDWSQRDKS-QSADAIAGKAF 648 Query: 240 ADLSKIRGARVFLMNPPAVRGLGQSSGFDLELKDVGGVGHDALLKARDQFLQLARGNALL 299 S+I+ A VF +NPP + LG ++GF L+D G+GH AL++AR+Q L +A + +L Sbjct: 649 GAFSQIKDAIVFPLNPPPIPELGNATGFTFRLQDRAGLGHTALVQARNQILGMASQSKVL 708 Query: 300 ANVRTTGLDDTPQLRVAIDDRKADALSIATSDINSTLATAMGGTYVNDFLSNGRVKKVYV 359 A VR GL+D PQL++ ID KA+AL + S INS L+TA+G +YVNDF + GR ++V V Sbjct: 709 AGVRPEGLEDAPQLQLDIDRDKANALGVPFSTINSVLSTALGSSYVNDFPNLGRQQRVIV 768 Query: 360 QGDSAFRMQAEDLNRWYVRNSASQMVPFSAFMSSKWTYGPSLLERYNGVSSFEIVGEPAA 419 Q D+ R+Q EDL Y RNS+ MVPFSAF+++KW GP L RYNG + +I G A Sbjct: 769 QADAKRRLQPEDLTNLYARNSSGTMVPFSAFVTAKWINGPVQLIRYNGYPAMKIAGGAAP 828 Query: 420 GVSSGTAMAEVEKLVAQLPAGIGFEWTGSSYQERLSGNQAPMLYAISILFVFLCLAALYE 479 G S+G AMAE+E VA+LP G GFEWTG S +E+ SGNQAP LYA+S++ VFL LAALYE Sbjct: 829 GRSTGEAMAEMESFVAKLPPGFGFEWTGQSLEEKTSGNQAPALYALSLIAVFLVLAALYE 888 Query: 480 SWSVPFAVILAVPLGIVGTVLLTGIFGMSNDVYFQVGLLTTVGLSAKNAILIVEFAKQLH 539 S S+P AV+L VPLGI+G +L + M NDVYF+VG++ VGLSAKNAILI+EFAK L Sbjct: 889 STSIPLAVMLVVPLGILGALLGVTLRAMPNDVYFKVGMIAVVGLSAKNAILIIEFAKDLQ 948 Query: 540 ESGKSLRDATLEAVQLRLRPILMTSLAFMFGVL 572 GK L +ATLEAV LR RPI+MTSLAF+ GVL Sbjct: 949 AQGKGLIEATLEAVHLRFRPIIMTSLAFILGVL 981 >gi|300313244|ref|YP_003777336.1| multidrug efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1033 Score = 514 bits (1325), Expect = e-147, Method: Compositional matrix adjust. Identities = 282/572 (49%), Positives = 377/572 (65%), Gaps = 3/572 (0%) Query: 1 VVVENVERLMSEEKLSPKEATRKSMQEITGALVGIAMVLSAVFIPMAFFGGSTGVIYRQF 60 VVVENVER+M+ E L P+EAT K+M EITGA++GI +VL+AVFIPMA GGS GVIYRQF Sbjct: 411 VVVENVERIMAHEGLGPREATIKAMGEITGAIIGITLVLTAVFIPMALAGGSVGVIYRQF 470 Query: 61 SITIVSAMALSVMVALTLTPALCATLLKPHASGHVQQGKFFTWFNRTFDRSATRYQGGVG 120 S+ + ++ S +AL+LTPALCATLL+P +GH + FF FNR F+R RY+ +G Sbjct: 471 SVAMAVSILFSAFLALSLTPALCATLLRPVPAGHHDKRGFFGAFNRAFNRLTARYESALG 530 Query: 121 GLLKRGKRGLLMYALIAVAMAVLFMRLPTSFLPEEDQGVLMAQIQLPTGATAEQTATVVK 180 L+ RG R +L+YA ++ + V LP++FLPEEDQG M QLP AT E+TA V Sbjct: 531 RLIARGLRMMLVYAALSGLLVVGMQALPSAFLPEEDQGYFMTSFQLPADATTERTARAVD 590 Query: 181 TVQEYFMNKPESVDSVLAIVGAGVGGNSQNGARAFIRLKEWDQRTGAGQSASELVRLANA 240 + Y + E + + L+++G G G+ N A AF LK+W +R G+ SA+E + A Sbjct: 591 QFERYLKGR-EDIGANLSVLGFGFSGSGPNAAMAFTELKDWKERKGS-TSAAEAMAAQAA 648 Query: 241 DLSKIRGARVFLMNPPAVRGLGQSSGFDLELKDVGGVGHDALLKARDQFLQLARGNALLA 300 S + G + L+ PPA+ LG SSGF + L+D G+ AL A Q L LA + L+ Sbjct: 649 MESGVDGMVMSLL-PPAIEELGNSSGFAMRLQDRANQGYAALKAAEAQLLALAAKSRKLS 707 Query: 301 NVRTTGLDDTPQLRVAIDDRKADALSIATSDINSTLATAMGGTYVNDFLSNGRVKKVYVQ 360 V + GL +++ ID KA AL ++ S I+ TL+ AMG YVNDF + GR+++V +Q Sbjct: 708 GVYSDGLPAGASVQLEIDRHKAAALGVSFSSISDTLSAAMGSFYVNDFPNAGRMQQVILQ 767 Query: 361 GDSAFRMQAEDLNRWYVRNSASQMVPFSAFMSSKWTYGPSLLERYNGVSSFEIVGEPAAG 420 ++ RMQ ED+ + YVRN + MVP + + + W P+ L+RY G + I G A G Sbjct: 768 AQASARMQLEDVMKLYVRNQSGGMVPLAELVKASWREAPTQLQRYQGFPAVRISGSAAPG 827 Query: 421 VSSGTAMAEVEKLVAQLPAGIGFEWTGSSYQERLSGNQAPMLYAISILFVFLCLAALYES 480 V+SG AMAE+E+L QLP G WTG S QERLS QAPML A+S+L VFL LAALYES Sbjct: 828 VASGEAMAEMERLAQQLPPGFAVAWTGQSLQERLSAAQAPMLMALSMLVVFLVLAALYES 887 Query: 481 WSVPFAVILAVPLGIVGTVLLTGIFGMSNDVYFQVGLLTTVGLSAKNAILIVEFAKQLHE 540 WS+P +V+L VPLG+VG V + M NDV+F+VG++T +GLSAKNAILIVEFA+QL Sbjct: 888 WSIPLSVMLVVPLGLVGAVAAVILRDMPNDVFFKVGMITVIGLSAKNAILIVEFARQLRS 947 Query: 541 SGKSLRDATLEAVQLRLRPILMTSLAFMFGVL 572 G L +A ++A LRLRPILMTSLAF GV+ Sbjct: 948 EGMGLVEAAIKASVLRLRPILMTSLAFALGVV 979 >gi|300309657|ref|YP_003773749.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1071 Score = 330 bits (847), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 228/589 (38%), Positives = 339/589 (57%), Gaps = 31/589 (5%) Query: 4 ENVERLMSEEKLSPKEATRKSMQEITGALVGIAMVLSAVFIPMAFFGGSTGVIYRQFSIT 63 ENVER + LSP++AT ++MQE++G ++ IA+ L AVF+P+AF G TG Y+QF++T Sbjct: 417 ENVERNIGA-GLSPRDATYRAMQEVSGPIIAIALTLVAVFVPLAFMTGLTGQFYKQFAMT 475 Query: 64 IVSAMALSVMVALTLTPALCATLLKPHASGHVQQGKFFTWFNRTFDR------------- 110 I + +S +LTL+PAL A LLK H G W R DR Sbjct: 476 IAISTVISAFNSLTLSPALAALLLKGH-------GDKPDWLTRQMDRFLGGFFIRFNRFF 528 Query: 111 --SATRYQGGVGGLLKRGKRGLLMYALIAVAMAVLFMRLPTSFLPEEDQGVLMAQIQLPT 168 ++ Y GV G++KR + +Y L+ A + +P F+P +D+ L+A QLP Sbjct: 529 NRASDNYGHGVTGVIKRKGATMGVYVLLLAATLGISYIVPGGFVPGQDKQYLVAFAQLPN 588 Query: 169 GATAEQTATVVKTVQEYFMNKPESVDSVLAIVGAGVGG--NSQNGARAFIRLKEWDQRTG 226 GA+ +++ V++ + + + +P V S +A G + G NS + F+ LK +++R Sbjct: 589 GASIDRSEEVMRKMSDIMLKEP-GVASAIAFPGLSINGFTNSSSAGIVFVGLKPFEERKS 647 Query: 227 AGQSASELVRLANADLSKIRGARVFLMNPPAVRGLGQSSGFDLELKDVGGVGHDALLKAR 286 S + + N I+ A + PP V GLG GF ++++D +G+ L KA Sbjct: 648 KDLSGNAIAASLNKKFGSIKEAFTAVFPPPPVMGLGTLGGFKMQIEDRDALGYAELDKAA 707 Query: 287 DQFLQLARGNALLANVRTTGLDDTPQLRVAIDDRKADALSIATSDINSTLATAMGGTYVN 346 F++ A L + ++ + PQL V +D KA L I +D+ +T+ +G YVN Sbjct: 708 QAFMKAAAKEPALGPMFSSYQINVPQLDVELDRVKAKQLGIPVTDVFNTMQIYLGSLYVN 767 Query: 347 DFLSNGRVKKVYVQGDSAFRMQAEDLNRWYVRNSASQMVPFSAFMSSKWTYGPSLLERYN 406 DF GRV +V Q D+ FR +AED+ + RN A +MVP S+ + K T+GP ++ RYN Sbjct: 768 DFNRFGRVYQVRAQADAPFRAKAEDIAQLKTRNDAGEMVPLSSVVKVKQTFGPEMVVRYN 827 Query: 407 GVSSFEIVGEPAAGVSSG-TAMAEVEKLVAQLPAGIGFEWTGSSYQERLSGNQAPMLYAI 465 G ++ +I G PA G SS A A LP GI FEWT +YQ+ L+GN ++ I Sbjct: 828 GYTAADINGGPAPGYSSDQAQAAAERVAAATLPRGIKFEWTDLTYQKILAGNAGVWVFPI 887 Query: 466 SILFVFLCLAALYESWSVPFAVILAVPLGIVGTVLLTGIFGM--SNDVYFQVGLLTTVGL 523 S+L VFL LAALYES ++P AVIL VP+ I+ LTG++ N+++ Q+GL+ VGL Sbjct: 888 SVLLVFLVLAALYESLTLPLAVILIVPMSILAA--LTGVWLTRGDNNIFTQIGLMVLVGL 945 Query: 524 SAKNAILIVEFAKQLHESGKSLRDATLEAVQLRLRPILMTSLAFMFGVL 572 SAKNAILIVEFA++L G++ A +EA +LRLRPILMTS+AF+ GV+ Sbjct: 946 SAKNAILIVEFARELEMQGRTAVQAAIEASRLRLRPILMTSIAFIMGVV 994 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,620,408 Number of extensions: 65249 Number of successful extensions: 326 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 298 Number of HSP's successfully gapped: 12 Length of query: 580 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 490 Effective length of database: 1,193,720 Effective search space: 584922800 Effective search space used: 584922800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)