BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0076.p (420 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump pr... 811 0.0 gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump pr... 516 e-147 gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 466 e-132 gi|300313244|ref|YP_003777336.1| multidrug efflux system transme... 433 e-122 gi|300309657|ref|YP_003773749.1| cation/multidrug efflux pump pr... 311 6e-86 >gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 811 bits (2096), Expect = 0.0, Method: Compositional matrix adjust. Identities = 404/409 (98%), Positives = 408/409 (99%) Query: 1 MARFFIDRPIFAWVIAIIIMLGGMMSIRSLSLEQYPDIAPPKVNIKATYTGASAKTLEDS 60 MARFFIDRPIFAWVIAIIIMLGGMMSIRSLSLEQYPDIAPPKVNIKATYTGASAKTLEDS Sbjct: 1 MARFFIDRPIFAWVIAIIIMLGGMMSIRSLSLEQYPDIAPPKVNIKATYTGASAKTLEDS 60 Query: 61 VTQVIEQQMKGLDNLQYMSASSSSAGSATVALTFTAGTNPDVAQMQVQNKLQQVTARLPQ 120 VTQVIEQQMKGLDNLQYMSASSSSAGSATV+LTFTAGTNPDVAQMQVQNKLQQVTARLPQ Sbjct: 61 VTQVIEQQMKGLDNLQYMSASSSSAGSATVSLTFTAGTNPDVAQMQVQNKLQQVTARLPQ 120 Query: 121 AVQNNGVTVTKASTDILMVLSLTSDDPRITSIDIGDFISSTLTDQISRVEGVGDVTAFGT 180 AVQNNGVTVTKASTDILMVLSLTSDDPRITSIDIGDFISSTLTDQISRVEGVGDVTAFGT Sbjct: 121 AVQNNGVTVTKASTDILMVLSLTSDDPRITSIDIGDFISSTLTDQISRVEGVGDVTAFGT 180 Query: 181 NYAMRIWIDPAKLQKYALMPSDVTSALEAQNTEVSAGEIGALPAQPGQQINATITARSKL 240 NYAMRIWIDPAKLQKYALMPSD+TSAL+AQNTEVSAGEIGALPAQPGQQINATITARSKL Sbjct: 181 NYAMRIWIDPAKLQKYALMPSDITSALQAQNTEVSAGEIGALPAQPGQQINATITARSKL 240 Query: 241 TTPDEFRNIIVKSSADGSVVKLSDVARVELGGESYQVASQQNGKPSSALAIQLATGANAL 300 TTPDEFRNIIVKSS DGSVVKLSDVARVELGGESYQVASQQNGKPSSALAIQLATGANAL Sbjct: 241 TTPDEFRNIIVKSSVDGSVVKLSDVARVELGGESYQVASQQNGKPSSALAIQLATGANAL 300 Query: 301 ATADAVKKKMAELEPYFPASMKLKTNIAYDTTPFVRVSLEEVVKTLIEAIVLVVLIMYLF 360 ATADAVKKK+AELEPYFPASMKLKTNIAYDTTPFVRVSLEEVVKTLIEAIVLVVLIMYLF Sbjct: 301 ATADAVKKKLAELEPYFPASMKLKTNIAYDTTPFVRVSLEEVVKTLIEAIVLVVLIMYLF 360 Query: 361 LQNIRATFIPAITVPVVLLGTFGILAMFGYSINTLTMFGMVLAIGLLVD 409 LQNIRATFIPAITVPVVLLGTFGILAMFGYSINTLTMFGMVLAIGLLVD Sbjct: 361 LQNIRATFIPAITVPVVLLGTFGILAMFGYSINTLTMFGMVLAIGLLVD 409 >gi|300310690|ref|YP_003774782.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1049 Score = 516 bits (1328), Expect = e-147, Method: Compositional matrix adjust. Identities = 255/409 (62%), Positives = 325/409 (79%), Gaps = 2/409 (0%) Query: 1 MARFFIDRPIFAWVIAIIIMLGGMMSIRSLSLEQYPDIAPPKVNIKATYTGASAKTLEDS 60 MARFFIDRPIFAWV+AII+ML G++S+ +L + QYP IAPP + I A Y GASAKTLED+ Sbjct: 1 MARFFIDRPIFAWVLAIIVMLAGIVSVLTLPIAQYPAIAPPAIAITANYPGASAKTLEDT 60 Query: 61 VTQVIEQQMKGLDNLQYMSASSSSAGSATVALTFTAGTNPDVAQMQVQNKLQQVTARLPQ 120 VTQ+IEQ+MKGLD L YM+++S S+GS T+ LTF GTNPD AQ+QVQNKL T LP Sbjct: 61 VTQIIEQKMKGLDRLSYMASTSESSGSVTITLTFENGTNPDTAQVQVQNKLSLATPLLPS 120 Query: 121 AVQNNGVTVTKASTDILMVLSLTSDDPRITSIDIGDFISSTLTDQISRVEGVGDVTAFGT 180 VQ G+TVTK++T+ L VL+ TS+D ++ D+ D++++ + D ISRVEGVGD T FG+ Sbjct: 121 EVQQQGITVTKSATNFLNVLAFTSEDGSMSGSDLSDYVAANVQDAISRVEGVGDTTLFGS 180 Query: 181 NYAMRIWIDPAKLQKYALMPSDVTSALEAQNTEVSAGEIGALPAQPGQQINATITARSKL 240 YAMRIW+DP KL Y L P DV +A++AQN +VS+G++G LPA QQINATITA+++L Sbjct: 181 QYAMRIWLDPNKLNSYGLTPLDVKNAIQAQNAQVSSGQLGGLPAAENQQINATITAQTRL 240 Query: 241 TTPDEFRNIIVKSSADGSVVKLSDVARVELGGESYQVASQQNGKPSSALAIQLATGANAL 300 T D+F+NI++++ G+ VKL DVAR+ELG ESY + NGKP++ LAI+LATGANAL Sbjct: 241 RTADQFQNIVLRTLTGGAQVKLRDVARIELGSESYTSVGRYNGKPAAGLAIKLATGANAL 300 Query: 301 ATADAVKKKMAELEPYFPASMKLKTNIAYDTTPFVRVSLEEVVKTLIEAIVLVVLIMYLF 360 T A+ K++AELE +FP MK++ YDTTPFVR+S+EEVV+TLIEA+VLV L+MYLF Sbjct: 301 DTVKAIDKRVAELEKFFPTGMKVQK--PYDTTPFVRISIEEVVRTLIEAVVLVFLVMYLF 358 Query: 361 LQNIRATFIPAITVPVVLLGTFGILAMFGYSINTLTMFGMVLAIGLLVD 409 LQN RAT IP I VPVVLLGTFG+LA+FG++INTLTMF MVLAIGLLVD Sbjct: 359 LQNFRATLIPTIAVPVVLLGTFGVLAVFGFTINTLTMFAMVLAIGLLVD 407 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 466 bits (1198), Expect = e-132, Method: Compositional matrix adjust. Identities = 225/409 (55%), Positives = 302/409 (73%), Gaps = 2/409 (0%) Query: 1 MARFFIDRPIFAWVIAIIIMLGGMMSIRSLSLEQYPDIAPPKVNIKATYTGASAKTLEDS 60 M +FFIDRP+FAWV+AI IML G ++I L + QYP+IAPP + + ATY GA+A+TL+DS Sbjct: 1 MGKFFIDRPVFAWVLAIFIMLAGALAITGLPVSQYPNIAPPTIVVTATYPGATAQTLDDS 60 Query: 61 VTQVIEQQMKGLDNLQYMSASSSSAGSATVALTFTAGTNPDVAQMQVQNKLQQVTARLPQ 120 VT +IEQ+M G D LQY+ + S + G + +TF+ TNPD+A + VQN+L++V ARLP Sbjct: 61 VTSLIEQEMNGADGLQYIESQSQANGQVQITITFSPETNPDLAAVDVQNRLKRVEARLPA 120 Query: 121 AVQNNGVTVTKASTDILMVLSLTSDDPRITSIDIGDFISSTLTDQISRVEGVGDVTAFGT 180 AV GV VTK+ ++ L+ + L+S D ++ + +GD++S + ++I RV GVG FGT Sbjct: 121 AVTQQGVQVTKSRSNFLLFIGLSSTDGKLDPVALGDYLSRNVLNEIKRVPGVGQAQLFGT 180 Query: 181 NYAMRIWIDPAKLQKYALMPSDVTSALEAQNTEVSAGEIGALPAQPGQQINATITARSKL 240 AMRIWIDP KL L P+DVT+A++AQN V+ G +GALP+ QQI AT+ +L Sbjct: 181 ERAMRIWIDPNKLIGLNLTPADVTAAIQAQNALVAGGTLGALPSPGTQQIAATVVVNGQL 240 Query: 241 TTPDEFRNIIVKSSADGSVVKLSDVARVELGGESYQVASQQNGKPSSALAIQLATGANAL 300 T+P++F NII++++A+G+ V+L DVARVE+GG+SY + + +GKPSS + +QL+ ANAL Sbjct: 241 TSPEQFGNIILRANANGATVRLRDVARVEVGGQSYDTSGRLDGKPSSFIGVQLSPTANAL 300 Query: 301 ATADAVKKKMAELEPYFPASMKLKTNIAYDTTPFVRVSLEEVVKTLIEAIVLVVLIMYLF 360 TA AV KKM EL YFP +K I YDT+ FV++S+EEVVKTLIEAIVLV L+M+LF Sbjct: 301 GTATAVHKKMEELAKYFPTGVKY--TIPYDTSKFVKISIEEVVKTLIEAIVLVFLVMFLF 358 Query: 361 LQNIRATFIPAITVPVVLLGTFGILAMFGYSINTLTMFGMVLAIGLLVD 409 LQNIR T IP I VPV LLGTF L +FG+SIN LTMFGMVLAIG+LVD Sbjct: 359 LQNIRYTIIPTIVVPVALLGTFATLLLFGFSINVLTMFGMVLAIGILVD 407 >gi|300313244|ref|YP_003777336.1| multidrug efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1033 Score = 433 bits (1113), Expect = e-122, Method: Compositional matrix adjust. Identities = 200/409 (48%), Positives = 297/409 (72%), Gaps = 2/409 (0%) Query: 1 MARFFIDRPIFAWVIAIIIMLGGMMSIRSLSLEQYPDIAPPKVNIKATYTGASAKTLEDS 60 M RFFIDRPIFAWV+A+ I+L G++++ L + +YP +APP V+I ATY GA+ +T+ D Sbjct: 1 MPRFFIDRPIFAWVVALFIVLFGVIAMSQLPIARYPSVAPPTVSINATYPGATPQTMNDG 60 Query: 61 VTQVIEQQMKGLDNLQYMSASSSSAGSATVALTFTAGTNPDVAQMQVQNKLQQVTARLPQ 120 V +IE+++ G+ NL Y +S+ ++G+A +++TF GT+P++AQ+ VQNKL+ + ARLPQ Sbjct: 61 VISLIERELSGVKNLLYFESSADTSGTAQISVTFKPGTDPELAQVDVQNKLKAIEARLPQ 120 Query: 121 AVQNNGVTVTKASTDILMVLSLTSDDPRITSIDIGDFISSTLTDQISRVEGVGDVTAFGT 180 V+ NGVTV A++ L+++SLTS D R + + D+++ + +++ R+EGVG V FG Sbjct: 121 TVRQNGVTVESAASSFLLMVSLTSVDGRYDEVRLNDYLARNIVEELRRMEGVGRVQLFGA 180 Query: 181 NYAMRIWIDPAKLQKYALMPSDVTSALEAQNTEVSAGEIGALPAQPGQQINATITARSKL 240 AMRIW+DP KL Y L +D+++A+ QN +++ G +G PA GQQI +T + +L Sbjct: 181 EQAMRIWLDPFKLNAYGLTVTDISTAISQQNAQIAPGRLGDAPAVRGQQIAMPLTVQGQL 240 Query: 241 TTPDEFRNIIVKSSADGSVVKLSDVARVELGGESYQVASQQNGKPSSALAIQLATGANAL 300 +P +F I++K+ ADGS V L DVARVELG +SY A+++NGKP+ A+QL+ GANA+ Sbjct: 241 QSPAQFAAIVLKAKADGSRVLLGDVARVELGAQSYAFANRENGKPAGVAAVQLSPGANAV 300 Query: 301 ATADAVKKKMAELEPYFPASMKLKTNIAYDTTPFVRVSLEEVVKTLIEAIVLVVLIMYLF 360 TA A++ +M EL P P M ++ Y+T PFV++S+++VV+TLIEA+VLV L+MYLF Sbjct: 301 RTAAAIQARMEELRPSLPQGMAF--SLPYNTAPFVKISIQKVVQTLIEAMVLVFLVMYLF 358 Query: 361 LQNIRATFIPAITVPVVLLGTFGILAMFGYSINTLTMFGMVLAIGLLVD 409 LQN+R T IPAI P+ LLGT ++ + GYS+N LTMFGMVLAIG++VD Sbjct: 359 LQNVRYTVIPAIVAPIALLGTLAVMQLAGYSVNVLTMFGMVLAIGIIVD 407 >gi|300309657|ref|YP_003773749.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1071 Score = 311 bits (797), Expect = 6e-86, Method: Compositional matrix adjust. Identities = 177/406 (43%), Positives = 274/406 (67%), Gaps = 3/406 (0%) Query: 1 MARFFIDRPIFAWVIAIIIMLGGMMSIRSLSLEQYPDIAPPKVNIKATYTGASAKTLEDS 60 +++FFIDRPIFA V++++++LGG++++ L + +YP++ PP V ++A Y GA+ K + ++ Sbjct: 3 ISKFFIDRPIFAGVLSVLLLLGGVLAMLQLPISEYPEVVPPSVVVRAQYPGANPKVIAET 62 Query: 61 VTQVIEQQMKGLDNLQYMSASSSSAGSATVALTFTAGTNPDVAQMQVQNKLQQVTARLPQ 120 V +E+Q+ G++N+ YM + ++S G+ T+ + F G +PD AQ VQN++ Q RLP+ Sbjct: 63 VASPLEEQINGVENMLYMQSQANSDGNLTITVNFRLGVDPDKAQQLVQNRVSQALPRLPE 122 Query: 121 AVQNNGVTVTKASTDILMVLSLTSDDPRITSIDIGDFISSTLTDQISRVEGVGDVTAFGT 180 VQ GVT K+S + MV+ LTS D R + + ++ + D+++R++GVG+V FG+ Sbjct: 123 DVQRLGVTTLKSSPTLTMVVHLTSPDNRYDTTYLRNYAVLNVKDRLARIQGVGEVGLFGS 182 Query: 181 -NYAMRIWIDPAKLQKYALMPSDVTSALEAQNTEVSAGEIGALPAQPGQQINATITARSK 239 NYAMR+W+DP K+ + L +DV A+ QN +V+AG IGA P+ P + ++ A+ + Sbjct: 183 GNYAMRVWLDPNKVAQRGLTATDVVRAIREQNVQVAAGVIGASPSSPETPLQLSVNAQGR 242 Query: 240 LTTPDEFRNIIVKSSADGSVVKLSDVARVELGGESYQVASQQNGKPSSALAIQLATGANA 299 L T EFR+II+K+S DG+ +L DVARVEL Y + S + KP+ A+ I A GANA Sbjct: 243 LKTEGEFRDIILKASPDGATTRLGDVARVELAASEYGLRSLLDNKPAVAIPIFQAPGANA 302 Query: 300 LATADAVKKKMAELEPYFPASMKLKTNIAYDTTPFVRVSLEEVVKTLIEAIVLVVLIMYL 359 L + V+ M EL FP+S+ +I YD T FVR S+E VV TL+EAI LVV+++ + Sbjct: 303 LDVSTQVRAAMKELSADFPSSVSY--SIVYDPTQFVRASIEAVVHTLLEAIALVVIVVII 360 Query: 360 FLQNIRATFIPAITVPVVLLGTFGILAMFGYSINTLTMFGMVLAIG 405 FLQ RA+ IP + VPV ++GTF ++ FGY+IN L++FGMVLAIG Sbjct: 361 FLQTWRASIIPLLAVPVSIIGTFSLMLGFGYTINALSLFGMVLAIG 406 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.130 0.359 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,081,871 Number of extensions: 37985 Number of successful extensions: 213 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 191 Number of HSP's successfully gapped: 11 Length of query: 420 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 332 Effective length of database: 1,203,190 Effective search space: 399459080 Effective search space used: 399459080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)