BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0075.p (442 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313894|ref|YP_003777986.1| RND family efflux transporter M... 759 0.0 gi|300310689|ref|YP_003774781.1| RND family efflux transporter M... 381 e-107 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 355 4e-99 gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbas... 130 2e-31 gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [... 129 6e-31 >gi|300313894|ref|YP_003777986.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 759 bits (1961), Expect = 0.0, Method: Compositional matrix adjust. Identities = 387/430 (90%), Positives = 407/430 (94%), Gaps = 2/430 (0%) Query: 13 MQENLSLPRRPGRSSSTAARLVLSLMLVAVLGACSKSGDQPKPAAQSEVGYVVVKAQRQA 72 M S PRR GR+SS ARL LSL+LVAVL ACS+SGDQPKP AQ+EVGYVVVKAQRQA Sbjct: 1 MHPTASTPRRQGRTSSATARLALSLILVAVLAACSRSGDQPKPTAQAEVGYVVVKAQRQA 60 Query: 73 LTTELPGRTTAYLAADIRPQVGGIIQKRLFKEGSNVKAGQPLYQIDPASYQAAYDSAKAS 132 LTTELPGRTTAYLAADIRPQVGGIIQKRLFKEGS VKAGQPLYQIDPASYQAAYDSA+AS Sbjct: 61 LTTELPGRTTAYLAADIRPQVGGIIQKRLFKEGSTVKAGQPLYQIDPASYQAAYDSARAS 120 Query: 133 LLKAQATLASSKLKADRYAELNKIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINL 192 LLKA+ATLASSKLKA+RYAELNKIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINL Sbjct: 121 LLKAEATLASSKLKAERYAELNKIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINL 180 Query: 193 QYTKILSPISGRIEKSSVTPGALVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELA 252 QYTKILSPISGRIEKS+VTPGALVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELA Sbjct: 181 QYTKILSPISGRIEKSAVTPGALVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELA 240 Query: 253 SGQIKRSGDNAAQVKILLEDGSTYERTGKLEFTGLSVDTGTGMITLRAVVPNPEGVLLPG 312 SGQI++SG+NAAQVKI+LEDGSTYER GKLEFTGLSVDTGTGMITLRAVVPNP+GVLLPG Sbjct: 241 SGQIRKSGENAAQVKIVLEDGSTYERPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPG 300 Query: 313 AYVRAVLEEGVDDHALLVPQKAISRDQTGAATALVLGNDGKVQQRLVTVARAVGNNWWVS 372 AYVRAVLEEGVDDHALL PQKA+SRDQTGAATALVLG DGKVQQRLV+VARAVGNNWWVS Sbjct: 301 AYVRAVLEEGVDDHALLAPQKAVSRDQTGAATALVLGADGKVQQRLVSVARAVGNNWWVS 360 Query: 373 RGLQQGDKLIVDGLQKVRTGDTPRAIDITDSLQKDLAREAASRAADPSLGGDADKAGESA 432 RGL++GDKLIVDGLQKVRTGD PRAIDITDSLQKDLAREAA+ AADPSLGGDA K +SA Sbjct: 361 RGLKEGDKLIVDGLQKVRTGDAPRAIDITDSLQKDLAREAAAAAADPSLGGDAGK--DSA 418 Query: 433 AAPATSVQSK 442 A AT+ QSK Sbjct: 419 PASATAAQSK 428 >gi|300310689|ref|YP_003774781.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 400 Score = 381 bits (978), Expect = e-107, Method: Compositional matrix adjust. Identities = 202/358 (56%), Positives = 266/358 (74%), Gaps = 2/358 (0%) Query: 36 SLMLVAVLGACSKSGDQPKPAAQSEV-GYVVVKAQRQALTTELPGRTTAYLAADIRPQVG 94 +L +++VL AC K PAA + V G V V AQ L TELPGRT+ Y AD+RPQV Sbjct: 15 ALAILSVLSACGKPPGGAPPAAGTPVLGVVTVHAQPVELHTELPGRTSPYQIADVRPQVN 74 Query: 95 GIIQKRLFKEGSNVKAGQPLYQIDPASYQAAYDSAKASLLKAQATLASSKLKADRYAELN 154 GII+ R F+EGS+VKAGQ LYQI+PASYQAAYDS+ A+L KA+A++ +++LKA RY EL Sbjct: 75 GIIKARSFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASVRTARLKASRYKELV 134 Query: 155 KIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINLQYTKILSPISGRIEKSSVTPGA 214 I AVS+QD DDA ++L + +ADV +A VQT+ INL Y ++ +PISGRI KSSVTPGA Sbjct: 135 AIKAVSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYARLDAPISGRIGKSSVTPGA 194 Query: 215 LVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELASGQIKRSGDNAAQVKILLEDGS 274 LVTA+QTTALTT+QQ+DPIYVDVTQS+ L L+ LA+GQ+++SG N A+V+++LE+G Sbjct: 195 LVTANQTTALTTIQQLDPIYVDVTQSSTTWLNLKEALANGQLEKSG-NGAKVRLVLENGR 253 Query: 275 TYERTGKLEFTGLSVDTGTGMITLRAVVPNPEGVLLPGAYVRAVLEEGVDDHALLVPQKA 334 TY + G L+F+ ++V+ TG ITLRA+ PNP+ LLPG YVRAVL+EGV LLVPQ+A Sbjct: 254 TYAQEGTLQFSDVTVNQDTGAITLRAIFPNPKTDLLPGMYVRAVLQEGVRQQGLLVPQQA 313 Query: 335 ISRDQTGAATALVLGNDGKVQQRLVTVARAVGNNWWVSRGLQQGDKLIVDGLQKVRTG 392 +SRD G A V+ G V+QR + RA+G+ W VS GL++GD+L+VDG Q+ G Sbjct: 314 VSRDGAGKPVAFVVNAQGVVEQRALVTDRAIGDQWLVSSGLKEGDQLVVDGQQRASAG 371 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 355 bits (911), Expect = 4e-99, Method: Compositional matrix adjust. Identities = 190/379 (50%), Positives = 259/379 (68%), Gaps = 12/379 (3%) Query: 26 SSSTAARLVLSLMLVAVLGACSKSGDQPKPAAQ------SEVGYVVVKAQRQALTTELPG 79 S S RL +++ + A L AC + KPAAQ SEV V + QR A+T ELPG Sbjct: 3 SVSRFTRLTMTVAIAAALVACGQ-----KPAAQGGPMPPSEVSVVTIAPQRVAITNELPG 57 Query: 80 RTTAYLAADIRPQVGGIIQKRLFKEGSNVKAGQPLYQIDPASYQAAYDSAKASLLKAQAT 139 R A+ A +R +V GI+QKR+FKEGS VK GQ L++IDPA Y+A+ +SA+A+L +AQA Sbjct: 58 RVEAFRVAQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQAN 117 Query: 140 LASSKLKADRYAELNKIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINLQYTKILS 199 L + L A RY L +++AVSKQ+ D+A + +Q +ADV KAAVQTA++NL Y + S Sbjct: 118 LTQTTLTAQRYKPLVEVNAVSKQEYDNAVAAQKQAEADVAAGKAAVQTASLNLGYATVTS 177 Query: 200 PISGRIEKSSVTPGALVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELASGQIKRS 259 PISGRI ++ VT GALV T L VQQ+DP+YV+VTQS+ DLL+L++ +A+GQ+K Sbjct: 178 PISGRIGRALVTEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKSV 237 Query: 260 GDNAAQVKILLEDGSTYERTGKLEFTGLSVDTGTGMITLRAVVPNPEGVLLPGAYVRAVL 319 GDN A V + LE+G Y GKL F+ +SVD TG ++LRA PNP+ +LLPG YVRA + Sbjct: 238 GDNKASVTLELENGQAYPLPGKLLFSDISVDENTGSVSLRAEFPNPKRLLLPGTYVRAKI 297 Query: 320 EEGVDDHALLVPQKAISRDQTGAATALVLGNDGKVQQRLVTVARAVGNNWWVSRGLQQGD 379 E+ VD+ ALLVPQ+A+ RD G +T +V+ + KV+ R V A GNNW V GL++GD Sbjct: 298 EQAVDEQALLVPQQAVVRD-AGGSTVMVVNAENKVEPRPVKTGGARGNNWQVLDGLKEGD 356 Query: 380 KLIVDGLQKVRTGDTPRAI 398 ++IV+GLQKVR G T + + Sbjct: 357 RVIVEGLQKVRPGATVKPV 375 >gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 130 bits (328), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 115/360 (31%), Positives = 171/360 (47%), Gaps = 31/360 (8%) Query: 47 SKSGDQPKPAAQSEVGYVVVKAQRQALTTELPG--RTTAYLAADIRPQVGGIIQKRLFKE 104 + + DQP+ A V + Q + L L G TA + ++ ++ G ++K F+E Sbjct: 35 ASAADQPQAKAPPPVSVTLASVQERDLPQYLAGVGTVTANASVVVKTRIDGQLEKVGFQE 94 Query: 105 GSNVKAGQPLYQIDPASYQAAYDSAKASLLKAQATLASSKLKADRYAELNKIDAVSKQDN 164 G +VK GQ L QID + QA A+A K ATL +++L RY L DA ++Q Sbjct: 95 GEDVKKGQLLAQIDARALQAQLAQAEAQRAKDSATLMNARLDLQRYTTLRSQDAATQQQL 154 Query: 165 DDAQSTLRQNQADVEVQKAAVQTAAINLQYTKILSPISGRIEKSSVTPGALVTASQTTAL 224 D ++ + Q +A V+ +A V A + L YT I +PISGR+ V PG +V A+ L Sbjct: 155 DTQKALVAQLEAAVKTDEAQVAYAKVQLSYTTINAPISGRVGARLVDPGNIVHATDANGL 214 Query: 225 TTVQQIDPIYVDVTQSTVDLLRLRRELASG--QIKRSGDNAAQVKILLEDGSTYERTGKL 282 + QIDPI + + L E +G + +R+ QV D TGKL Sbjct: 215 VVINQIDPITL--------VFTLPEEAVAGINRAQRAHQQPLQVSAYARDSQEVLATGKL 266 Query: 283 EFTGLSVDTGTGMITLRAVVPNPEGVLLPGAYVRAVLEEGVDDHALLVPQKAISRDQTGA 342 ++T G + L+A NP+ L PG YV A L+ +AL VP AI R+Q G Sbjct: 267 VLLDNQINTTNGTVQLKARFDNPDHKLWPGQYVNARLQMRSHGNALTVPAAAIQRNQQG- 325 Query: 343 ATALVLGNDGKVQQRL--VTVAR--------AVGNNWWVSRGLQQGDKLIVDGLQKVRTG 392 V D + Q R+ V V R A GN +Q G ++++DG K++ G Sbjct: 326 --TYVYAIDQEDQARMQPVKVERIQDGIAVLAAGN------AVQAGQRVVIDGQYKLKPG 377 >gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [Herbaspirillum seropedicae SmR1] Length = 399 Score = 129 bits (323), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 102/323 (31%), Positives = 161/323 (49%), Gaps = 10/323 (3%) Query: 79 GRTTAYLAADIRPQVGGIIQKRLFKEGSNVKAGQPLYQIDPASYQAAYDSAKASLLKAQA 138 GR A ++RP VGG + FK+G+ VK G L+ IDP Y+A A+A L A+A Sbjct: 69 GRIEAVDRVEVRPLVGGTLTAVHFKDGALVKKGDVLFTIDPRPYEAEVARAEAQLAGAEA 128 Query: 139 TLASSKLKADRYAELNKIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINLQYTKIL 198 + R L +A++K+D ++ Q+ R+ A+V +AA++ A +NL+YT+I Sbjct: 129 RAGYTASDLARGQRLLSDNAIAKRDFEEKQNASREASANVAAARAALRAARLNLEYTRIT 188 Query: 199 SPISGRIEKSSVTPGALVTASQTTA-LTTVQQIDPIYVDVTQSTVDLLRLRRELASGQIK 257 +P+SGRI ++ VT G +V A LTT+ + +Y L+ Sbjct: 189 APVSGRISRAEVTVGNVVNPGAANAPLTTLVSVAKVYASFDVDEQSFLKYVNP------A 242 Query: 258 RSGDNAAQVKILLEDGSTYERTGKLEFTGLSVDTGTGMITLRAVVPNPEGVLLPGAYVRA 317 R+ V++ L + Y R G++ +DT +G I +RA+ N L+PG Y R Sbjct: 243 RAAGATVPVELGLANEDGYSRKGRVGSVDNRLDTASGTIRVRAIFDNANSQLVPGLYARV 302 Query: 318 VLEEGVDDHALLVPQKAISRDQTGAATALVLGNDGKVQQRLVTVARAVGNNWWVSRGLQQ 377 L G A+L+ +KAI DQ +V D R V V + +GLQ Sbjct: 303 RLGGGEPHQAVLIDEKAIGTDQD-KRFVMVADKDNHANYRQVVVGAGHDGLVVIEKGLQP 361 Query: 378 GDKLIVDGLQKVRTGD--TPRAI 398 G++++V+GLQ++R GD TP + Sbjct: 362 GERIVVNGLQRIRPGDAITPTVV 384 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.127 0.342 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,258,858 Number of extensions: 51452 Number of successful extensions: 222 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 209 Number of HSP's successfully gapped: 12 Length of query: 442 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 354 Effective length of database: 1,203,190 Effective search space: 425929260 Effective search space used: 425929260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)