BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0074.p (484 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313895|ref|YP_003777987.1| two-component system sensor his... 888 0.0 gi|300311272|ref|YP_003775364.1| two component sensor histidine ... 144 1e-35 gi|300313246|ref|YP_003777338.1| two component transmembrane sen... 142 5e-35 gi|300311514|ref|YP_003775606.1| two component sensor histidine ... 121 1e-28 gi|300313666|ref|YP_003777758.1| two component sensor histidine ... 114 1e-26 >gi|300313895|ref|YP_003777987.1| two-component system sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 888 bits (2295), Expect = 0.0, Method: Compositional matrix adjust. Identities = 431/475 (90%), Positives = 456/475 (96%) Query: 10 LTDRFFYRANIRLKLFYAMLALIVAVIAAINGAGHYIFNRDFLGYLNEQAQQRMEDMVPR 69 ++DRFFYRANIRLKLFYAMLALIVA+IAAINGAGHYIFNRDFLGYLNEQAQQRMEDMVPR Sbjct: 1 MSDRFFYRANIRLKLFYAMLALIVALIAAINGAGHYIFNRDFLGYLNEQAQQRMEDMVPR 60 Query: 70 LEQAYREHGNWEFLRDNPRAWFELMKPAIVEHSGAVRTSGPTFTPSDLTGATLRFSLLDA 129 LEQAYREHG+W+F+RDNPRAWFELMKPAIVEH+GA R++GPTFT SDLTGATLRFSLLDA Sbjct: 61 LEQAYREHGSWDFIRDNPRAWFELMKPAIVEHNGAARSAGPTFTASDLTGATLRFSLLDA 120 Query: 130 RRQLVIGYPDVGPDARLSPLMQDDVIVGWVAQTPFESVIGSIDLRFQQSQFLAQIIIAVV 189 R+ VIGYP+VGPDARL PL+QDDVI+GWVAQTPFESVIG++DLRFQQSQF AQIIIAVV Sbjct: 121 SRRPVIGYPNVGPDARLRPLLQDDVIIGWVAQTPFESVIGALDLRFQQSQFAAQIIIAVV 180 Query: 190 AVMLAAFLVVRISGVLTRPLTRVARATHRLAGGDYAIRVEVESGDEVGMLAQDFNQLAMT 249 AVMLAAFLVVRISGVLTRPLTRVARATHRLA GDYAIRVEVESGDEVGMLAQDFNQLAMT Sbjct: 181 AVMLAAFLVVRISGVLTRPLTRVARATHRLASGDYAIRVEVESGDEVGMLAQDFNQLAMT 240 Query: 250 LERNEKLRREFMADVSHELRTPLGILNGQLEALEDGVLQPDAQTLRSLKSEVEHLNKLVG 309 LERNEKLRREFMADVSHELRTPL ILNGQLEALEDGVLQPDAQTLRSLKSEVEHLNKLVG Sbjct: 241 LERNEKLRREFMADVSHELRTPLAILNGQLEALEDGVLQPDAQTLRSLKSEVEHLNKLVG 300 Query: 310 DLYDLSLADAGALTYRRTDIDLAPLLRDSAAIYAERFSQRGMTLTCSLPATMPVHADAAR 369 DLYDLSLADAGALTYRR DIDLAPLLRDSAA+Y++RFSQRGM L+C LPA+MPVH DAAR Sbjct: 301 DLYDLSLADAGALTYRRADIDLAPLLRDSAALYSDRFSQRGMRLSCELPASMPVHGDAAR 360 Query: 370 LQQLLNNLFENSLRYTDSGGRLQVHAQLLQQHWHLVFDDSEPGVPPEVMARLFDRFFRVE 429 LQQLL+NLFENSLRYTD+GGRLQVHA L QQHWHLVFDDSEPGVPPEVMARLF+RFFRVE Sbjct: 361 LQQLLSNLFENSLRYTDAGGRLQVHAHLQQQHWHLVFDDSEPGVPPEVMARLFERFFRVE 420 Query: 430 ASRNRASGGAGLGLAICRRIVDAHQGTIGATASPLGGLRVSVVLPARAENEEDRA 484 ASRNRASGGAGLGLAICRRIVDAHQGTIGA+ASPLGGLRV VVLPAR E+E A Sbjct: 421 ASRNRASGGAGLGLAICRRIVDAHQGTIGASASPLGGLRVVVVLPARLEDERGSA 475 >gi|300311272|ref|YP_003775364.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 144 bits (363), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 107/303 (35%), Positives = 160/303 (52%), Gaps = 6/303 (1%) Query: 177 QSQFLAQIIIAVVAVMLAAFLVVRISGVLTRPLTRVARATHRLAGGDYAIRV----EVES 232 Q+ L ++ ++A+ L + +S L+R L VA A RLA GD++ R +V + Sbjct: 93 QADLLWFSLLVLIALPLIVGAGLMLSLRLSRHLDAVAVAARRLAAGDFSARALSCKQVPA 152 Query: 233 GDEVGMLAQDFNQLAMTLERNEKLRREFMADVSHELRTPLGILNGQLEALEDGVLQPDAQ 292 + L +D N++A LER E+ A ++HELRTPL +L+ + DG+ Q Sbjct: 153 A--LDALMRDVNEMAERLERRERELHASSAAIAHELRTPLTAAKARLQGIMDGIFDRSPQ 210 Query: 293 TLRSLKSEVEHLNKLVGDLYDLSLADAGALTYRRTDIDLAPLLRDSAAIYAERFSQRGMT 352 L + +++ LN+L+ DLY LSL AG L R+ L PLL + A A M+ Sbjct: 211 QLSMIMRQLDQLNRLISDLYLLSLVSAGQLALVRSQFQLRPLLEERLAWAAPSLRLLPMS 270 Query: 353 LTCSLPATMPVHADAARLQQLLNNLFENSLRYTDSGGRLQVHAQLLQQHWHLVFDDSEPG 412 +P++AD RL Q ++ L +N+LRY SG L + A L H+ +D PG Sbjct: 271 TALYCCEHLPLNADRDRLGQAISILIDNALRYAASGKLLTISAGLHDGQIHIDVEDDGPG 330 Query: 413 VPPEVMARLFDRFFRVEASRNRASGGAGLGLAICRRIVDAHQGTIGATASPLGGLRVSVV 472 PE + DRF+R E SR+R +GG+GLGLA+ I +AH G + P GG R+ + Sbjct: 331 FAPEHLELACDRFWRAEHSRSRHAGGSGLGLAVAVAICEAHGGKLSVHNRPAGGARMHLE 390 Query: 473 LPA 475 LPA Sbjct: 391 LPA 393 >gi|300313246|ref|YP_003777338.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 361 Score = 142 bits (358), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 98/293 (33%), Positives = 147/293 (50%), Gaps = 4/293 (1%) Query: 184 IIIAVVAVMLAAFLVVRISGVLTRPLTRVARATHRLAGGDYAIRVEVESG--DEVGMLAQ 241 ++ ++ + +A ++ VR+S + PL VA + R+A GD + R E DE L Sbjct: 70 LVTTLIGLSIALWVAVRLSRRILTPLNSVATSLRRVAQGDLSARAAAERQPLDEAWQLIN 129 Query: 242 DFNQLAMTLERNEKLRREFMADVSHELRTPLGILNGQLEALEDGVLQPDAQTLRSLKSEV 301 DFN +A LER ++ R + A ++HELRTP+ IL G+L+ L DGV QP+ SL ++V Sbjct: 130 DFNAMAERLERVQQERIFWNAAIAHELRTPVTILRGRLQGLSDGVFQPEPALFSSLLAQV 189 Query: 302 EHLNKLVGDLYDLSLADAGALTYRRTDIDLAPLLRDSAAIYAERFSQRGMTLTCSLPATM 361 E L +L+ DL + LA++G L + + DL + ++G L Sbjct: 190 EGLGRLIEDLRIVGLAESGHLRVQLQETDLQADIMTVLKACEPALQEKGFRLLLE-TGVQ 248 Query: 362 PVHADAARLQQLLNNLFENSLRYTDSGGRLQVHAQLLQQHWHLVFDDSEPGVPPEVMARL 421 D R++Q L L EN L++ S G L++ + L L +DS PG+ + Sbjct: 249 RSRCDPVRIRQALLALLENVLQHA-SPGPLRISTRSLAGMHSLCVEDSGPGIAASDAQMI 307 Query: 422 FDRFFRVEASRNRASGGAGLGLAICRRIVDAHQGTIGATASPLGGLRVSVVLP 474 FD F R + + G+GLGLA+ R I AH GT AS LGG V P Sbjct: 308 FDAFHRGARAGDAQQKGSGLGLAVVRTIASAHHGTADCRASELGGACFEVRWP 360 >gi|300311514|ref|YP_003775606.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 473 Score = 121 bits (304), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 95/313 (30%), Positives = 154/313 (49%), Gaps = 13/313 (4%) Query: 174 RFQQSQFLAQIIIAVVAVMLAAFLVVRISGVLTRPLTRVARATHRLAGGDYAIRVE---V 230 R +S F + I VVA++ + + ++ + P+ + A ++ A G+ + +E Sbjct: 156 RGPKSMFFPTMPI-VVALLASLVFALLLAWYFSHPIGHLKNAFNQAANGNLDLELEPVMS 214 Query: 231 ESGDEVGMLAQDFNQLAMTLERNEKLRREFMADVSHELRTPLGILNGQLEALEDGVLQPD 290 DE+ L +DF+ + L + +R + DVSHELR+P+ L + Sbjct: 215 ARNDELADLGRDFDVMTAKLRTLMESQRRLLHDVSHELRSPIARLQVAIGLARVQDKDKL 274 Query: 291 AQTLRSLKSEVEHLNKLVGDLYDLSLADAGALTYRRTDIDLAPLLRD---SAAIYAERFS 347 TL ++ E ++KLVG+L LS +A I + +L D A+ AE Sbjct: 275 DLTLERIERESVRMDKLVGELLTLSRLEANVPGSMEEPIAIEAMLGDMINDASFEAEAI- 333 Query: 348 QRGMTLTCSLPATMPVHADAARLQQLLNNLFENSLRYTDSGGRLQVHAQL--LQQHWHLV 405 G + L + V + L + + N+ N++++T G +Q+H QL + + HL Sbjct: 334 --GKHVDDQLDCRVQVKGNPELLYRAIENVLRNAIKHTAPGTTVQIHTQLDEINKLLHLS 391 Query: 406 FDDSEPGVPPEVMARLFDRFFRVEASRNRASGGAGLGLAICRRIVDAHQGTIGATASPLG 465 D PGVP E M +F+ FFR +++ +SG GLGL+I RRIV+AHQG I A G Sbjct: 392 ILDRGPGVPEEEMNSIFELFFRSSRTKSNSSG-HGLGLSIARRIVEAHQGFIQAANRSNG 450 Query: 466 GLRVSVVLPARAE 478 GLRV + LP A+ Sbjct: 451 GLRVDITLPFMAD 463 >gi|300313666|ref|YP_003777758.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 479 Score = 114 bits (286), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 90/295 (30%), Positives = 146/295 (49%), Gaps = 19/295 (6%) Query: 180 FLAQIIIAVVAVMLAAFLVVRISGVLTRPLTRVARATHRLAGGDYAIRVEVESGDEVGML 239 F+ + + AV+L A++ VR++ +PL +A A +L G + + + EV Sbjct: 203 FMRIYALRIFAVVLIAWMAVRLA---MKPLQVMAEAAEKLGGNIRSAPLATDGPLEVSTA 259 Query: 240 AQDFNQLAMTLERNEKLRREFMADVSHELRTPLGILNGQLEALEDGVLQPDAQTLRSLKS 299 AQ FN + + N R F+A VSH+LR+P+ + + E L PD + + Sbjct: 260 AQAFNAMQRRIIDNIAERTRFLAAVSHDLRSPITRMKLRTEML------PDERGKEKFRK 313 Query: 300 EVEHLNKLVGDLYDLSLADAGALTYRRTDIDLAPLLRDSAAIYAERFSQRGMTLTCSLPA 359 +++ ++ L+ L++ + R IDL LL AE G +T S A Sbjct: 314 DLDEMDALIS--ATLNVVQDIDVQERPQLIDLDSLLESLRTDQAEV----GGEVTLSGHA 367 Query: 360 TMPVHADAARLQQLLNNLFENSLRYTDSGGRLQVHAQLLQQHWHLVFDDSEPGVPPEVMA 419 P+ L++ L NL EN++RY G R +V Q + DD PG+P ++ Sbjct: 368 A-PLQGYPRSLRRCLQNLVENAVRY---GQRARVQLQDDADQVCIRVDDDGPGIPEALLE 423 Query: 420 RLFDRFFRVEASRNRASGGAGLGLAICRRIVDAHQGTIGATASPLGGLRVSVVLP 474 R+ + F+R+E SRN +GG GLGL+I +V AH+G + P GGLRV +++P Sbjct: 424 RVQEPFYRLERSRNAGTGGFGLGLSIAATVVQAHRGVMKLVNRPGGGLRVEILIP 478 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,467,312 Number of extensions: 61569 Number of successful extensions: 374 Number of sequences better than 1.0e-08: 40 Number of HSP's gapped: 311 Number of HSP's successfully gapped: 40 Length of query: 484 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 395 Effective length of database: 1,198,455 Effective search space: 473389725 Effective search space used: 473389725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)