BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0073.p (247 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313896|ref|YP_003777988.1| two component transcription res... 433 e-123 gi|300309361|ref|YP_003773453.1| response regulator for cobalt/z... 152 2e-38 gi|300313245|ref|YP_003777337.1| two component response regulato... 150 1e-37 gi|300310592|ref|YP_003774684.1| phosphate regulon two-component... 150 1e-37 gi|300312356|ref|YP_003776448.1| cobalt/zinc/cadmium resistance ... 146 2e-36 >gi|300313896|ref|YP_003777988.1| two component transcription response regulator protein [Herbaspirillum seropedicae SmR1] Length = 244 Score = 433 bits (1113), Expect = e-123, Method: Compositional matrix adjust. Identities = 217/247 (87%), Positives = 228/247 (92%), Gaps = 5/247 (2%) Query: 1 MNESTDDFRFAAPERADILIVEDEPKLAELLQKYLAVAGYTSRHVARGDAALDAVRAQRP 60 M++ T +F+FA P ADILIVEDEPKLAELLQKYLA+AGY++RHVARGDAALDAVRAQRP Sbjct: 1 MSDPTTEFQFAPPASADILIVEDEPKLAELLQKYLALAGYSARHVARGDAALDAVRAQRP 60 Query: 61 DLILLDIMLPGMDGWEICRQLRTFSDVPVLMLTARAEEEDRLRGLELGADDYIGKTPFSP 120 DLILLDIMLPGMDGWEICRQLRTFSDVPVLMLTARAEEEDRLRGLELGADDYIGKTPFSP Sbjct: 61 DLILLDIMLPGMDGWEICRQLRTFSDVPVLMLTARAEEEDRLRGLELGADDYIGKTPFSP 120 Query: 121 REIVARVKSMLRRNASV-QRSAQAEPLPQGSSPLIIDETRHQASVRGEPLNLTPLELRLL 179 REIVARVKSMLRRNA V QR+AQA S L ID+ RHQASVRGE LNLTPLELRLL Sbjct: 121 REIVARVKSMLRRNALVQQRTAQAP----ARSLLHIDDVRHQASVRGEALNLTPLELRLL 176 Query: 180 KTFAAAPGQVFSREQLLNHLHDDDRSVTDRAIDTHIKNLRRKLEPCFPGHNAIQAVYGVG 239 KTFA+APGQVFSREQLLNHLHDDDRSVTDRA+DTHIKNLRRKLEPCFPGHNAIQAVYGVG Sbjct: 177 KTFASAPGQVFSREQLLNHLHDDDRSVTDRAVDTHIKNLRRKLEPCFPGHNAIQAVYGVG 236 Query: 240 YCFALPP 246 YCF LPP Sbjct: 237 YCFELPP 243 >gi|300309361|ref|YP_003773453.1| response regulator for cobalt/zinc/cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 152 bits (384), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 88/225 (39%), Positives = 129/225 (57%), Gaps = 8/225 (3%) Query: 18 ILIVEDEPKLAELLQKYLAVAGYTSRHVARGDAALDAVRAQRPDLILLDIMLPGMDGWEI 77 IL+VEDEPK + L++ L +G+ V G L R LI+LD+MLPGM+GW+I Sbjct: 3 ILLVEDEPKSGDYLRRGLTESGFVVDWVQTGVDGLHNARTLDYKLIILDVMLPGMNGWDI 62 Query: 78 CRQLRTFSDVPVLMLTARAEEEDRLRGLELGADDYIGKTPFSPREIVARVKSMLRRNASV 137 R+LR D PVL LTAR E EDR++GLELGADDY+ K PF+ E++ARV+++LRR S Sbjct: 63 LRELRRTHDTPVLFLTARDEVEDRVKGLELGADDYLVK-PFAYTELLARVRTLLRRGPSR 121 Query: 138 QRSAQAEPLPQGSSPLIIDETRHQASVRGEPLNLTPLELRLLKTFAAAPGQVFSREQLLN 197 + +++D R + + G+ ++LT E LL PG+V SR Q+ + Sbjct: 122 EVDLIE------YDDVVLDVLRRKVTRAGQRIDLTAKEFALLHMLIRRPGEVLSRPQIAS 175 Query: 198 HLHDDDRSVTDRAIDTHIKNLRRKLEPCFPGHNAIQAVYGVGYCF 242 + D + +D ++ LR KL+ F + V+G+GY F Sbjct: 176 QVWDMNFDSDTNVVDVAVRRLRAKLDEGFE-KKLLHTVWGMGYVF 219 >gi|300313245|ref|YP_003777337.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 267 Score = 150 bits (378), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 97/232 (41%), Positives = 142/232 (61%), Gaps = 11/232 (4%) Query: 16 ADILIVEDEPKLAELLQKYLAVAGYTSRHVARGDAALDAVRAQRPDLILLDIMLPGMDGW 75 A ILI ED P++AE+L YLA G S H A G AL+ A +PDL+LLDI +P ++GW Sbjct: 38 ALILIAEDAPEIAEILAAYLARNGMRSVHAADGARALELHLALKPDLVLLDIQMPQVNGW 97 Query: 76 EICRQLRTFSDVPVLMLTARAEEEDRLRGLELGADDYIGKTPFSPREIVARVKSMLRRNA 135 ++ Q+R + PV++LTA ++ D+L GL +GADDY+ K PF+P E+VARV+++LRR Sbjct: 98 QVLSQIRQRDNTPVILLTALDQDVDKLTGLRIGADDYVVK-PFNPAEVVARVEAVLRR-- 154 Query: 136 SVQRSAQAEPLPQGSSPLIIDETRHQ--ASVRG--EPLNLTPLELRLLKTFAAAPGQVFS 191 S+ R + + P +ID +H+ A + G L LT E +LL A AP +VFS Sbjct: 155 SLPREERDDRQVLRVPPFVIDFEQHEVVAEIEGVSHTLVLTLTEFKLLAQLARAPRRVFS 214 Query: 192 REQLLNHLHDDDRSVTDRAIDTHIKNLRRKLEPCFPGHNAI-QAVYGVGYCF 242 R +LL + ++ +R +D+H+ LR+KLE G I ++ GVGY F Sbjct: 215 RAELLASCLPEGDTL-ERTVDSHVSKLRKKLEDL--GIAGIPVSIRGVGYRF 263 >gi|300310592|ref|YP_003774684.1| phosphate regulon two-component response regulator transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 245 Score = 150 bits (378), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 90/229 (39%), Positives = 132/229 (57%), Gaps = 12/229 (5%) Query: 18 ILIVEDEPKLAELLQKYLAVAGYTSRHVARGDAALDAVRAQRPDLILLDIMLPGMDGWEI 77 ILIVEDEP +AEL+ L AG++S V+ AA D V+ + P L+LLD MLP G + Sbjct: 24 ILIVEDEPSIAELIAFTLKEAGWSSFVVSNAAAAWDFVQHRAPQLVLLDWMLPDRSGLHL 83 Query: 78 CRQLRT---FSDVPVLMLTARAEEEDRLRGLELGADDYIGKTPFSPREIVARVKSMLRRN 134 ++R + V+MLTA++ EED++ GL+ GADDYI K PFSPRE+ AR+K++LRR Sbjct: 84 LSRIRADRQLQQLSVIMLTAKSMEEDKIAGLDNGADDYITK-PFSPRELTARIKALLRR- 141 Query: 135 ASVQRSAQAEPLPQGSSPLIIDETRHQASVRGEPLNLTPLELRLLKTFAAAPGQVFSREQ 194 +S + + P+ +D S+ G +++ E +LLK F A P +VFSR Q Sbjct: 142 ----KSPEHAQEVMSAGPITLDPASCSVSLSGARIDIGHAEYKLLKFFLAHPDRVFSRSQ 197 Query: 195 LLNHLHDDDRSVTDRAIDTHIKNLRRKLEPCFPGHNAIQAVYGVGYCFA 243 LL+ + D + +R +D H+ LR+ L I+ V VGY + Sbjct: 198 LLDRVWGDHVVIEERTVDVHVLRLRKALR---DAEYLIKTVRSVGYMLS 243 >gi|300312356|ref|YP_003776448.1| cobalt/zinc/cadmium resistance two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 223 Score = 146 bits (368), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 87/229 (37%), Positives = 127/229 (55%), Gaps = 8/229 (3%) Query: 18 ILIVEDEPKLAELLQKYLAVAGYTSRHVARGDAALDAVRAQRPDLILLDIMLPGMDGWEI 77 IL++ED+PK + L+K L +GY G L Q DL++LD+MLPG+DGW++ Sbjct: 3 ILVIEDDPKTGDYLRKGLRESGYAVDLARNGADGLHMALEQDYDLVVLDVMLPGLDGWQV 62 Query: 78 CRQLRTFSDVPVLMLTARAEEEDRLRGLELGADDYIGKTPFSPREIVARVKSMLRRNASV 137 LR+ D+PVL LTAR +DR+RGL+LGADDY+ K PFS E+V R++++LRR V Sbjct: 63 MSALRSKRDLPVLFLTARDHVDDRIRGLQLGADDYLVK-PFSFTELVLRIRTLLRR--GV 119 Query: 138 QRSAQAEPLPQGSSPLIIDETRHQASVRGEPLNLTPLELRLLKTFAAAPGQVFSREQLLN 197 R A+ + + L +D RH+ + +G + LT E LL G+ SR + + Sbjct: 120 TREAEVYEI----ADLQLDHVRHKVTRQGVNIALTNKEFMLLHLLIKRQGEALSRSVIAS 175 Query: 198 HLHDDDRSVTDRAIDTHIKNLRRKLEPCFPGHNAIQAVYGVGYCFALPP 246 + D + +D IK LR K++ F I V VGY F+ P Sbjct: 176 QIWDMNFDSDTNVVDVAIKRLRAKIDAPFD-KKLIHTVRSVGYMFSEEP 223 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 682,437 Number of extensions: 23624 Number of successful extensions: 245 Number of sequences better than 1.0e-08: 42 Number of HSP's gapped: 202 Number of HSP's successfully gapped: 42 Length of query: 247 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 164 Effective length of database: 1,226,865 Effective search space: 201205860 Effective search space used: 201205860 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)