BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0071.p (436 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 613 e-176 gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspiri... 227 1e-60 gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprot... 227 2e-60 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 219 4e-58 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 184 1e-47 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 613 bits (1580), Expect = e-176, Method: Compositional matrix adjust. Identities = 339/455 (74%), Positives = 369/455 (81%), Gaps = 58/455 (12%) Query: 23 ACASGPDYVRPQVDMPAAWT--------------PEAPW---------RAMQPQ------ 53 CASGPDY RPQVDMPAAWT P PW A+Q Q Sbjct: 18 GCASGPDYTRPQVDMPAAWTSEAPWRSMQPADATPRGPWWERFGDAQLNALQQQALAGNQ 77 Query: 54 -------------------DAAPPPPPP----------PPXRPLTNYNSPNFATVQNDFA 84 AA P RPLTNYNSPNF+TVQNDFA Sbjct: 78 TLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSRISANRPLTNYNSPNFSTVQNDFA 137 Query: 85 LGLNVSYELDFFGRVRRSVEAATASAQQSAADLENTRLLLTAELASNYFNLRELDIELDV 144 LG +VSYE+DFFGRV+RSVEAA A+A+QSAADLENTRLLLTAELA+NYFNLRELD+ELDV Sbjct: 138 LGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATNYFNLRELDVELDV 197 Query: 145 VRRAIELQRKALELATARHDLGATSGLDVAQQQALLDNTLTQVDLLGRQRAQYEHAIATL 204 VRRAI LQR+ALELATARHDLGATSGLD+AQQQALLD+TLTQ+DLLGRQRAQYEHAIATL Sbjct: 198 VRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLGRQRAQYEHAIATL 257 Query: 205 TGTPAPAFSLPPSLTPIALPEIPLGIPSDVLERRPDVAAAERSMAAANAQIGVASAAFYP 264 TGTPAPAFS+ PSLTPI P IP+G+PSDVLERRPD+AAAER+MAAANAQIGVASAA+YP Sbjct: 258 TGTPAPAFSITPSLTPITPPAIPVGVPSDVLERRPDIAAAERAMAAANAQIGVASAAYYP 317 Query: 265 SFMLQPGYGVDSRNWATLFNAPSVLWSLGVSASQSLFDAGRLRAGVDFTQAGYNATVATY 324 SFMLQP YGVDSRNWATLFNAPS+LWSLGVSASQSLFD GRLRAGVDF+QAGY ATVA+Y Sbjct: 318 SFMLQPSYGVDSRNWATLFNAPSLLWSLGVSASQSLFDGGRLRAGVDFSQAGYEATVASY 377 Query: 325 RRTVLTAMQEVEDGITGIASLDRAWRQSQSAIASARRVLDIATSRYEGGATPYLDVITAQ 384 RRTVLTAMQEVEDGITG+A+L+RA+ QSQ AIASARRVL+IA+SRYEGGATPYLDVITAQ Sbjct: 378 RRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIASSRYEGGATPYLDVITAQ 437 Query: 385 QALLNSERQAAQLMGQRLLVSVFLIKALGGDWHAR 419 Q+LLNSERQAAQLMGQRLLVSVFLIKALGGDW +R Sbjct: 438 QSLLNSERQAAQLMGQRLLVSVFLIKALGGDWQSR 472 >gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 507 Score = 227 bits (579), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 139/341 (40%), Positives = 201/341 (58%) Query: 79 VQNDFALGLNVSYELDFFGRVRRSVEAATASAQQSAADLENTRLLLTAELASNYFNLREL 138 V+N + L ++E D +G+VRR VE++ ASAQ SAADL RL A++A NY LR L Sbjct: 141 VRNSYTFSLTAAWEADIWGKVRRDVESSQASAQASAADLAAARLSAQAQVAQNYLQLRVL 200 Query: 139 DIELDVVRRAIELQRKALELATARHDLGATSGLDVAQQQALLDNTLTQVDLLGRQRAQYE 198 D + ++ ++ ++L++A ++ G + DV Q Q L + G RAQ E Sbjct: 201 DAQQQLLEETVQAYERSLQVAQNQYAAGMVAKSDVIQAQTQLKSAQASALDNGVTRAQVE 260 Query: 199 HAIATLTGTPAPAFSLPPSLTPIALPEIPLGIPSDVLERRPDVAAAERSMAAANAQIGVA 258 HAIA L G F+LP + P +P G+PS +LERRPDVAAAER +A+ANA+IGVA Sbjct: 261 HAIALLVGKTPAQFALPRAPLAAVAPPVPQGVPSMLLERRPDVAAAERRVASANAKIGVA 320 Query: 259 SAAFYPSFMLQPGYGVDSRNWATLFNAPSVLWSLGVSASQSLFDAGRLRAGVDFTQAGYN 318 AA++PS L G S +A F P +WSLG +Q++FD G A D A Y+ Sbjct: 321 KAAYFPSLTLSASGGYQSSTFADWFTLPGRVWSLGPQLAQTIFDGGARAAATDQAIATYD 380 Query: 319 ATVATYRRTVLTAMQEVEDGITGIASLDRAWRQSQSAIASARRVLDIATSRYEGGATPYL 378 TVATY++TVLTA QEVED + + L++ A+ +AR+ + + ++Y+ G Y+ Sbjct: 381 GTVATYKQTVLTAFQEVEDNLAALRILEQEAAVQGEALQAARQAVTLVNNQYKAGTVSYV 440 Query: 379 DVITAQQALLNSERQAAQLMGQRLLVSVFLIKALGGDWHAR 419 +VITAQ L++E + ++ +R+ SV L KALGG W R Sbjct: 441 NVITAQATALSAELSSLNILNRRMAASVLLAKALGGGWDER 481 >gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprotein [Herbaspirillum seropedicae SmR1] Length = 510 Score = 227 bits (578), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 179/473 (37%), Positives = 243/473 (51%), Gaps = 66/473 (13%) Query: 14 ALASALLLAACASGPDYVRPQVDMPAA------WTPEAP---------WRA--------- 49 AL A LL CA GPDY RP +D+P A W AP W A Sbjct: 37 ALVLAALLGGCAVGPDYARPALDLPQAYKETGPWKLAAPRQIDDHQPWWEAYGDPVLNAL 96 Query: 50 MQPQDAAP-------------------------PPPPPPPXRPLTNYNSPNFATVQNDFA 84 M +AA P N+ + N ++ Sbjct: 97 MDQANAANQTIQQAAAQYRQAQATAEVARASLWPTIGAQAGASRAQTNTNGVQRLGNSYS 156 Query: 85 LGLNVSYELDFFGRVRRSVEAATASAQQSAADLENTRLLLTAELASNYFNLRELDIELDV 144 +GLN S+E D +GR+RR EA A+AQ SAA L RL + A LA +Y LR D++ D+ Sbjct: 157 VGLNASWEADLWGRIRRGAEAGEANAQASAASLAAARLSIQATLAQDYLQLRVTDLQKDL 216 Query: 145 VRRAIELQRKALELATARHDLGATSGLDVAQQQALLDNTLTQVDLLGRQRAQYEHAIATL 204 RR + ++L+L+T +++ G DVAQ + L Q+ L R Q EHAIA L Sbjct: 217 YRRTVAAYTRSLQLSTHQYEAGTALRSDVAQAETQLRAAQAQLIDLDDTRNQLEHAIAML 276 Query: 205 TGTPAPAFSLPPSLTPI----------------ALPEIPLGIPSDVLERRPDVAAAERSM 248 G FSLP +L P +LP+IP+G+PS++LERRPD+A AER Sbjct: 277 IGKAPAQFSLP-ALPPAAASDSAIDANAARLQGSLPQIPVGLPSELLERRPDIANAERLA 335 Query: 249 AAANAQIGVASAAFYPSFMLQPGYGVDSRNWATLFNAPSVLWSLGVSASQSLFDAGRLRA 308 AAANA IGVA AA++P+ L G +S +A LFN PS +WSLG + ++SLFD G A Sbjct: 336 AAANANIGVARAAYFPTLTLSASGGYNSLAFANLFNTPSRVWSLGSALAESLFDGGARSA 395 Query: 309 GVDFTQAGYNATVATYRRTVLTAMQEVEDGITGIASLDRAWRQSQSAIASARRVLDIATS 368 D A Y+A VA Y++TVL +QEVED ++ + LD+ A+ SA+ +A Sbjct: 396 RNDAAVAAYDAAVAQYKQTVLGGLQEVEDKLSTLRVLDQESTVQAQAVQSAQLAERLAMR 455 Query: 369 RYEGGATPYLDVITAQQALLNSERQAAQLMGQRLLVSVFLIKALGGDWHARRE 421 +YE G YL V+T Q A L ++R A L+G++L+ SV LIKA GG W A R+ Sbjct: 456 QYEAGTVTYLSVVTTQAASLTNQRNAVTLLGRQLVASVALIKATGGGWQAGRD 508 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 219 bits (557), Expect = 4e-58, Method: Compositional matrix adjust. Identities = 163/391 (41%), Positives = 224/391 (57%), Gaps = 28/391 (7%) Query: 33 PQVDMPAAWTPEAPWRAMQ--PQDAAPPPPPPPPXRPLTNYNSPNFATVQNDFALGLNVS 90 PQVD T + P A Q P DA RP T Y + +NVS Sbjct: 124 PQVDAGVGATRQRPSPASQGLPADA--------DTRPNTLYRAQ------------VNVS 163 Query: 91 YELDFFGRVRRSVEAATASAQQSAADLENTRLLLTAELASNYFNLRELDIELDVVRRAIE 150 YE+D FGRV +V+AATA AQ+S A + L L A++A YF +RELD + R ++ Sbjct: 164 YEVDLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQQYFLVRELDAAAALYRGTVD 223 Query: 151 LQRKALELATARHDLGATSGLDVAQQQALLDNTLTQVDLLGRQRAQYEHAIATLTGTPAP 210 L++++L L R D G S +DVA+ +A L + ++ + RQRA EHA+A L G Sbjct: 224 LRQESLRLVQRRFDEGDISEVDVARARAELASAQSEALGIARQRATAEHALAILLGRTPA 283 Query: 211 AFSLPPS-LTPIALPEIPLGIPSDVLERRPDVAAAERSMAAANAQIGVASAAFYPSFMLQ 269 FSLPP LT +A+ +P G+PS +LERRPD+AAAER+MAAANA++GVA +AF+PS L Sbjct: 284 EFSLPPQPLTRLAVT-VPPGLPSALLERRPDIAAAERAMAAANARVGVAKSAFFPSLNLT 342 Query: 270 PGYGVDSRNWATLFNAPSVLWSL----GVSASQSLFDAGRLRAGVDFTQAGYNATVATYR 325 G G +S LFN S + L G + S LFD GR +AGVD +A Y VA+YR Sbjct: 343 GGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPLFDGGRRQAGVDRARASYEEQVASYR 402 Query: 326 RTVLTAMQEVEDGITGIASLDRAWRQSQSAIASARRVLDIATSRYEGGATPYLDVITAQQ 385 TVL A +EVED + + LD+ R A+A++ R ++ ++Y GA Y VI A + Sbjct: 403 GTVLNAFREVEDNLANLRILDQQNRAQDEAVAASARAAKLSHTQYREGAVSYFSVIDADR 462 Query: 386 ALLNSERQAAQLMGQRLLVSVFLIKALGGDW 416 +L +R + L G+R +V LI+ALGG W Sbjct: 463 TVLQQQRASVTLDGERARSTVNLIRALGGGW 493 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 184 bits (467), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 120/344 (34%), Positives = 184/344 (53%), Gaps = 6/344 (1%) Query: 78 TVQNDFALGLNV-SYELDFFGRVRRSVEAATASAQQSAADLENTRLLLTAELASNYFNLR 136 T+ N + +GL V +YELDFFGRVR +AA AS + ++ ++ L A++A Y R Sbjct: 132 TISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQAYLAER 191 Query: 137 ELDIELDVVRRAIELQRKALELATARHDLGATSGLDVAQQQALLDNTLTQVDLLGRQRAQ 196 +L + ++ ++ L+LA R D+GA++ LD Q L+++ L RQRAQ Sbjct: 192 SYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARVAKTQLERQRAQ 251 Query: 197 YEHAIATLTGTPAPAFSLPPSL---TPIALPEIPLGIPSDVLERRPDVAAAERSMAAANA 253 E+A+ L G LPP+ + +IP G+PSD+L RPD+ +AE+S+ AANA Sbjct: 252 AENALTLLVGKKIG--ELPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSAEQSLLAANA 309 Query: 254 QIGVASAAFYPSFMLQPGYGVDSRNWATLFNAPSVLWSLGVSASQSLFDAGRLRAGVDFT 313 IG A AAF+P L +G S LF + S WS G S S +FD GR A +D Sbjct: 310 NIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRNSANLDLA 369 Query: 314 QAGYNATVATYRRTVLTAMQEVEDGITGIASLDRAWRQSQSAIASARRVLDIATSRYEGG 373 + N V TY + + TA +EV D + SLD + ++ +A+ + L + R++ G Sbjct: 370 EVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERLKLTDLRFKNG 429 Query: 374 ATPYLDVITAQQALLNSERQAAQLMGQRLLVSVFLIKALGGDWH 417 LD + AQ+ L ++++ Q RL ++ L KALGG + Sbjct: 430 IASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLN 473 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.130 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,217,984 Number of extensions: 46804 Number of successful extensions: 281 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 249 Number of HSP's successfully gapped: 18 Length of query: 436 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 348 Effective length of database: 1,203,190 Effective search space: 418710120 Effective search space used: 418710120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)