BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0070.p (407 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313899|ref|YP_003777991.1| RND family efflux transporter M... 677 0.0 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 112 7e-26 gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbas... 99 7e-22 gi|300310851|ref|YP_003774943.1| RND family efflux transporter M... 96 8e-21 gi|300313894|ref|YP_003777986.1| RND family efflux transporter M... 95 9e-21 >gi|300313899|ref|YP_003777991.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 406 Score = 677 bits (1748), Expect = 0.0, Method: Compositional matrix adjust. Identities = 364/406 (89%), Positives = 390/406 (96%) Query: 1 MSHDRHSELGIHPLGEGEPQQRQQMMKRARLVLVVVLVLLAAGAARTLVSRAANARALEA 60 MSHDRHSELGIHPL EGEPQQRQQMM+RAR+VLVVVL+LLAAGAART++SR ANAR L+A Sbjct: 1 MSHDRHSELGIHPLSEGEPQQRQQMMRRARVVLVVVLLLLAAGAARTVISRVANARELQA 60 Query: 61 GTAERAKIFVRVAPARSSAEGQTLALPGSLQGFVQSPISARSGGYLKRWTKDIGSEVKKG 120 GTAERAK+FVRVAP RSSAEGQTLALPG+LQGFVQSPISARSGGYLKRWTKDIG+EVKKG Sbjct: 61 GTAERAKMFVRVAPVRSSAEGQTLALPGTLQGFVQSPISARSGGYLKRWTKDIGAEVKKG 120 Query: 121 ELLAELDTPEIDQQLSQALAARQQAASSMELARSSMARWEALRKKDAVSQQELDERRSAF 180 ELLAELDTPEIDQQL+QALAAR+QAASSMELARSSMARWEALRKKDAVSQQELDERRSAF Sbjct: 121 ELLAELDTPEIDQQLTQALAAREQAASSMELARSSMARWEALRKKDAVSQQELDERRSAF 180 Query: 181 AQASSNLAAADANVERLRQVEGFKRIVAPFSGIITRRNVDVGDLIDPGAGRALFVLSQTD 240 AQAS+NLAAADANV RLRQVEGFKRIVAPFSGIITRRNVDVGDLIDPGAGRALFVLSQTD Sbjct: 181 AQASANLAAADANVGRLRQVEGFKRIVAPFSGIITRRNVDVGDLIDPGAGRALFVLSQTD 240 Query: 241 PLRVYVNVPQAYANLVKPGQQVTVTQSELRGRKFEGKVARTAGSIDAATRSMQIEVTLSN 300 PLRVYVNVPQ+YANLVKPGQQVTVTQSELRGRKFEGKVARTAG+IDAATRSMQ+EVTLSN Sbjct: 241 PLRVYVNVPQSYANLVKPGQQVTVTQSELRGRKFEGKVARTAGAIDAATRSMQVEVTLSN 300 Query: 301 ADNALLPGAFVQVELPLKPSGTLLVAADTLMFRRDGSLVAAVDEQNKVRLKKVRLGRNFG 360 AD+ALLPGAFVQV LP+KPSG LLVAADTLMFRRDG+LVAAVD N+VRLKK+RLGRNFG Sbjct: 301 ADHALLPGAFVQVALPIKPSGALLVAADTLMFRRDGTLVAAVDPHNQVRLKKLRLGRNFG 360 Query: 361 LTVEVLEGLKGDEKLILNPSDSLAEGDQVEPTLDQPKDKAADKKAA 406 TVEVL+GL+GDE LILNPSD+LAEGDQVEPTL+QPK+ K A+ Sbjct: 361 QTVEVLDGLQGDEHLILNPSDALAEGDQVEPTLEQPKEPEKQKAAS 406 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 112 bits (279), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 100/344 (29%), Positives = 164/344 (47%), Gaps = 21/344 (6%) Query: 69 FVRVAPARSSAEGQTLALPGSLQGFVQSPISARSGGYLKRWTKDIGSEVKKGELLAELDT 128 V +AP R + T LPG ++ F + + AR G +++ GSEVK+G++L ++D Sbjct: 41 VVTIAPQRVAI---TNELPGRVEAFRVAQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDP 97 Query: 129 PEIDQQLSQALAARQQAASSMELARSSMARWEALRKKDAVSQQELDERRSAFAQASSNLA 188 + L A A +A +++ + R++ L + +AVS+QE D +A QA +++A Sbjct: 98 AQYKASLESAQATLARAQANLTQTTLTAQRYKPLVEVNAVSKQEYDNAVAAQKQAEADVA 157 Query: 189 AADANVERLRQVEGFKRIVAPFSGIITRRNVDVGDLIDPGAGRALFVLSQTDPLRVYVNV 248 A A V+ G+ + +P SG I R V G L+ G L ++ Q DP VYVNV Sbjct: 158 AGKAAVQTASLNLGYATVTSPISGRIGRALVTEGALVGQGDATQLALVQQLDP--VYVNV 215 Query: 249 PQAYANLVKPGQ---------------QVTVTQSELRGRKFEGKVARTAGSIDAATRSMQ 293 Q+ A+L+K Q VT+ + GK+ + S+D T S+ Sbjct: 216 TQSSADLLKLQQAMAAGQLKSVGDNKASVTLELENGQAYPLPGKLLFSDISVDENTGSVS 275 Query: 294 IEVTLSNADNALLPGAFVQVELPLK-PSGTLLVAADTLMFRRDGSLVAAVDEQNKVRLKK 352 + N LLPG +V+ ++ LLV ++ GS V V+ +NKV + Sbjct: 276 LRAEFPNPKRLLLPGTYVRAKIEQAVDEQALLVPQQAVVRDAGGSTVMVVNAENKVEPRP 335 Query: 353 VRLGRNFGLTVEVLEGLKGDEKLILNPSDSLAEGDQVEPTLDQP 396 V+ G G +VL+GLK +++I+ + G V+P P Sbjct: 336 VKTGGARGNNWQVLDGLKEGDRVIVEGLQKVRPGATVKPVQWNP 379 >gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 99.0 bits (245), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 77/284 (27%), Positives = 142/284 (50%), Gaps = 8/284 (2%) Query: 82 QTLALPGSLQGFVQSPISARSGGYLKRWTKDIGSEVKKGELLAELDTPEIDQQLSQALAA 141 Q LA G++ + R G L++ G +VKKG+LLA++D + QL+QA A Sbjct: 63 QYLAGVGTVTANASVVVKTRIDGQLEKVGFQEGEDVKKGQLLAQIDARALQAQLAQAEAQ 122 Query: 142 RQQAASSMELARSSMARWEALRKKDAVSQQELDERRSAFAQASSNLAAADANVERLRQVE 201 R + ++++ AR + R+ LR +DA +QQ+LD +++ AQ + + +A V + Sbjct: 123 RAKDSATLMNARLDLQRYTTLRSQDAATQQQLDTQKALVAQLEAAVKTDEAQVAYAKVQL 182 Query: 202 GFKRIVAPFSGIITRRNVDVGDLIDPGAGRALFVLSQTDPLRVYVNVPQAYANLVKPGQ- 260 + I AP SG + R VD G+++ L V++Q DP+ + +P+ + Q Sbjct: 183 SYTTINAPISGRVGARLVDPGNIVHATDANGLVVINQIDPITLVFTLPEEAVAGINRAQR 242 Query: 261 ------QVTVTQSELRGRKFEGKVARTAGSIDAATRSMQIEVTLSNADNALLPGAFVQVE 314 QV+ + + GK+ I+ ++Q++ N D+ L PG +V Sbjct: 243 AHQQPLQVSAYARDSQEVLATGKLVLLDNQINTTNGTVQLKARFDNPDHKLWPGQYVNAR 302 Query: 315 LPLKPSGT-LLVAADTLMFRRDGSLVAAVDEQNKVRLKKVRLGR 357 L ++ G L V A + + G+ V A+D++++ R++ V++ R Sbjct: 303 LQMRSHGNALTVPAAAIQRNQQGTYVYAIDQEDQARMQPVKVER 346 >gi|300310851|ref|YP_003774943.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 95.5 bits (236), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 82/326 (25%), Positives = 149/326 (45%), Gaps = 13/326 (3%) Query: 88 GSLQGFVQSPISARSGGYLKRWTKDIGSEVKKGELLAELDTPEIDQQLSQALAARQQAAS 147 G++ + + AR G L + G VK G+LLAE+D + L+QA + + Sbjct: 52 GAVTPTATATVRARVDGQLMKLHYKEGQVVKAGDLLAEIDPRSLQAALTQAEGQLARDRA 111 Query: 148 SMELARSSMARWEALRKKDAVSQQELDERRSAFAQASSNLAAADANVERLRQVEGFKRIV 207 + AR + R++ L +D+++ Q++D + + Q + A + NV R F R+ Sbjct: 112 LLASARLDLKRYQTLLAQDSIASQQVDTQVALVKQYEGTVKADEGNVASARLQLSFTRVT 171 Query: 208 APFSGIITRRNVDVGDLIDPGAGRALFVLSQTDPLRVYVNVPQAYANLVKPGQQVTVTQS 267 AP SG + R DVG+ + L +++Q P+ ++P+ N+ K QQ+ + Sbjct: 172 APISGRLGLRQADVGNNVTTSDTNGLVIITQLQPITAIFSIPE--DNIPKVLQQLQSGRK 229 Query: 268 --------ELRGRKFEGKVARTAGSIDAATRSMQIEVTLSNADNALLPGAFVQVELPLKP 319 E + + +G + +DA T +++++ N D AL P FV + L L Sbjct: 230 LPAQAWDREQKNKLADGVLLTIDNVVDATTGTVKLKAQFPNTDYALFPSQFVNIRLQLNT 289 Query: 320 S-GTLLVAADTLMFRRDGSLVAAVDEQNKVRLKKVRLGR-NFGLTVEVLEGLKGDEKLIL 377 G ++ + G V V + + V ++ V+ G LTV + +G+ E +++ Sbjct: 290 EQGATVIPTAAIQRGSKGLFVYVVKDDSSVTVRPVKTGPVQDDLTV-ITDGVSAGETVVI 348 Query: 378 NPSDSLAEGDQVEPTLDQPKDKAADK 403 + D L EG +VE D A+K Sbjct: 349 DGIDRLREGAKVEAVARGGADDPANK 374 >gi|300313894|ref|YP_003777986.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 95.1 bits (235), Expect = 9e-21, Method: Compositional matrix adjust. Identities = 106/394 (26%), Positives = 176/394 (44%), Gaps = 30/394 (7%) Query: 11 IHPLGEGEPQQRQQMMKRARLVLVVVLVLLAAGAARTLVSRAANARALEAGTAERAKIFV 70 +HP +Q + ARL L ++LV + A +R+ A+A E G +V Sbjct: 1 MHPTASTPRRQGRTSSATARLALSLILVAVLAACSRSGDQPKPTAQA-EVG-------YV 52 Query: 71 RVAPARSSAEGQTLALPGSLQGFVQSPISARSGGYLKRWTKDIGSEVKKGELLAELDTPE 130 V R + T LPG ++ + I + GG +++ GS VK G+ L ++D Sbjct: 53 VVKAQR---QALTTELPGRTTAYLAADIRPQVGGIIQKRLFKEGSTVKAGQPLYQIDPAS 109 Query: 131 IDQQLSQALAARQQAASSMELARSSMARWEALRKKDAVSQQELDERRSAFAQASSNLAAA 190 A A+ +A +++ ++ R+ L K DAVS+Q+ D+ +S Q +++ Sbjct: 110 YQAAYDSARASLLKAEATLASSKLKAERYAELNKIDAVSKQDNDDAQSTLRQNQADVEVQ 169 Query: 191 DANVERLRQVEGFKRIVAPFSGIITRRNVDVGDLIDPGAGRALFVLSQTDPLRVYVNVPQ 250 A V+ + +I++P SG I + V G L+ AL + Q DP +YV+V Q Sbjct: 170 KAAVQTAAINLQYTKILSPISGRIEKSAVTPGALVTASQTTALTTVQQIDP--IYVDVTQ 227 Query: 251 AYANLV------------KPGQQVTVTQSELR-GRKFE--GKVARTAGSIDAATRSMQIE 295 + +L+ K G+ + L G +E GK+ T S+D T + + Sbjct: 228 STVDLLRLRRELASGQIRKSGENAAQVKIVLEDGSTYERPGKLEFTGLSVDTGTGMITLR 287 Query: 296 VTLSNADNALLPGAFVQVELPLKPSGTLLVAADTLMFR-RDGSLVAAV-DEQNKVRLKKV 353 + N D LLPGA+V+ L L+A + R + G+ A V KV+ + V Sbjct: 288 AVVPNPDGVLLPGAYVRAVLEEGVDDHALLAPQKAVSRDQTGAATALVLGADGKVQQRLV 347 Query: 354 RLGRNFGLTVEVLEGLKGDEKLILNPSDSLAEGD 387 + R G V GLK +KLI++ + GD Sbjct: 348 SVARAVGNNWWVSRGLKEGDKLIVDGLQKVRTGD 381 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.130 0.349 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,033,588 Number of extensions: 37095 Number of successful extensions: 173 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 164 Number of HSP's successfully gapped: 11 Length of query: 407 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 320 Effective length of database: 1,207,925 Effective search space: 386536000 Effective search space used: 386536000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)