BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0054.p (272 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313913|ref|YP_003778005.1| short-chain dehydrogenase [Herb... 483 e-137 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 89 4e-19 gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogena... 84 1e-17 gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogena... 82 5e-17 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 80 1e-16 >gi|300313913|ref|YP_003778005.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 270 Score = 483 bits (1242), Expect = e-137, Method: Compositional matrix adjust. Identities = 241/266 (90%), Positives = 250/266 (93%) Query: 1 MNQPHTQVRRGTALVTGASSGIGAIYADRLARLGYDLILVARNRDRLNTLAARITTETAR 60 MNQPHT RGTALVTGASSGIGAIYADRLARLGYDLILVARNR RLNTLA RIT +T R Sbjct: 1 MNQPHTPAHRGTALVTGASSGIGAIYADRLARLGYDLILVARNRSRLNTLAERITNQTGR 60 Query: 61 NVEVLEADLGQAASLAQVEEKLRSDASITLLVNNAGIGTHTPLLQSKVEHMTDMIALNVT 120 +VEVLEADLGQAASLAQVEEKLRSDASITLLVNNAGIGTHTPLLQS VEHMT MIALNVT Sbjct: 61 SVEVLEADLGQAASLAQVEEKLRSDASITLLVNNAGIGTHTPLLQSDVEHMTGMIALNVT 120 Query: 121 APTRLTYAAVPGFVARGRGAVINISSIVAVAPEVLNGVYGGSKAFVLAFSQSLHHELAAQ 180 APTRLTYAAVPGFVARG+GAVINISSIV VAPEVLNGVYGGSKAFVLAFSQSLHHELAAQ Sbjct: 121 APTRLTYAAVPGFVARGQGAVINISSIVGVAPEVLNGVYGGSKAFVLAFSQSLHHELAAQ 180 Query: 181 GVQVQAVLPGATATDFWAIGGLPVEHLDPAIVMSAEQMVDAALVGFQRGELVTIPSLHDE 240 GVQVQAVLPGATATDFWAIGGLPVEHLDPAIVMSAEQMVDAALVGFQR ELVTIPSLH+E Sbjct: 181 GVQVQAVLPGATATDFWAIGGLPVEHLDPAIVMSAEQMVDAALVGFQRRELVTIPSLHEE 240 Query: 241 TAWTRFDSARQQMATQLSANTPAPRY 266 AWT +++ARQ + +LSANTPAPRY Sbjct: 241 DAWTGYEAARQALIARLSANTPAPRY 266 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 89.0 bits (219), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 66/194 (34%), Positives = 101/194 (52%), Gaps = 7/194 (3%) Query: 13 ALVTGASSGIGAIYADRLARLGYDLILVARNRDRLNTLAARITTETARNVEVLEADLGQA 72 AL+TGAS+GIG A AR G LIL AR +D L +AA I + R V+ D+G+A Sbjct: 9 ALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGR-ASVVAGDVGEA 67 Query: 73 ASLAQ-VEEKLRSDASITLLVNNAG-IGTHTPLLQSKVEHMTDMIALNVTAPTRLTYAAV 130 A+ + V L + + +NNAG +G PL + + D + N+T+ + Sbjct: 68 ATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAFLGARCQI 127 Query: 131 PGFVARGRGAVINISSIVAVAPEVLN-GVYGGSKAFVLAFSQSLHHELAAQGVQVQAVLP 189 P +ARG GA++ SS V + + YG +KA ++ + + + A QG++ A+LP Sbjct: 128 PAMLARGAGAIVFTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQGIRANALLP 187 Query: 190 GATATDFWAIGGLP 203 G TD +GG P Sbjct: 188 GGVDTD---MGGDP 198 >gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 248 Score = 84.0 bits (206), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 70/203 (34%), Positives = 100/203 (49%), Gaps = 26/203 (12%) Query: 7 QVRRGTALVTGASSGIGAIYADRLARLGYDLILVARNRDRLNTLAARIT----------T 56 ++ ++TGASSG+G A LA LG L+L AR +RL L A IT T Sbjct: 4 NIKDKVVVITGASSGLGETTARHLASLGAKLVLGARRTERLEKLVADITAAGGQAIAVTT 63 Query: 57 ETARNVEVLEADLGQAASLAQVEEKLRSDASITLLVNNAGIGTHTPLLQSKVEHMTDMIA 116 + AR +V A +A+ E+ I +LVNNAGI P+ + KVE MI Sbjct: 64 DVARRDDV-------EALVAKGEQHF---GRIDVLVNNAGIMPLAPMAKLKVEEWDRMID 113 Query: 117 LNVTAPTRLTYAAVPGFVARGRGAVINISSIVAVAPEVLNG---VYGGSKAFVLAFSQSL 173 +NV AA+P F A+ G +IN+SS+ + +V G VY +K V A ++ L Sbjct: 114 VNVKGVLYGVAAALPRFAAQSSGHIINVSSVAGI--KVFAGMGTVYSATKFAVRALTEGL 171 Query: 174 HHELAAQGVQVQAVLPGATATDF 196 E A GV+ + PGA ++ Sbjct: 172 RTE-APDGVRTTIISPGAVDSEL 193 >gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 82.0 bits (201), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 60/189 (31%), Positives = 93/189 (49%), Gaps = 12/189 (6%) Query: 14 LVTGASSGIGAIYADRLARLGYDLILVARNRDRLNTLAARITTETARNVEVLEADLGQAA 73 L+TGA++G GA A + A G+ +I R ++RL+ L + + +VL L Sbjct: 4 LITGATAGFGAAMARKFAGNGHKVIAAGRRKERLDDLVKELGAD-----KVLPLVL-DVT 57 Query: 74 SLAQVEEKLRS----DASITLLVNNAGIGTHT-PLLQSKVEHMTDMIALNVTAPTRLTYA 128 S + +E L S + +L+NNAG+ P + + MI NV +T+A Sbjct: 58 SKSSIETGLASLTGAWKEVDVLINNAGLALGLGPAHEVALSDWETMIDTNVKGLVTITHA 117 Query: 129 AVPGFVARGRGAVINISSIVAVAPEVLNGVYGGSKAFVLAFSQSLHHELAAQGVQVQAVL 188 +P V RGRG +IN+ S+ P VYG +KAFV F+ +L +L GV+ + Sbjct: 118 LLPDMVKRGRGTIINLGSVAGAYPYPGGNVYGATKAFVDQFTLNLRADLVGTGVRATNLA 177 Query: 189 PG-ATATDF 196 PG T+F Sbjct: 178 PGLCGGTEF 186 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 80.5 bits (197), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 68/189 (35%), Positives = 98/189 (51%), Gaps = 9/189 (4%) Query: 13 ALVTGASSGIGAIYADRLARLGYDLILVARNRD---RLNTLAARITTETARNVEVLEADL 69 ALVTGAS GIG A LAR G +I A + + A + E R VL + Sbjct: 11 ALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAGRGA-VLNVND 69 Query: 70 GQAASLAQVEEKLRSDASITLLVNNAGIGTHTPLLQSKVEHMTDMIALNVTAPTRLTYAA 129 +A S+A VE+ + S+++LVNNAGI ++ K E +IA N+TA RL+ A Sbjct: 70 AEA-SVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRLSRAV 128 Query: 130 VPGFVARGRGAVINISSIVAVA--PEVLNGVYGGSKAFVLAFSQSLHHELAAQGVQVQAV 187 + G + G +INI+S+V A P +N Y +KA V S++L E+ ++ + V V Sbjct: 129 LRGMMKARTGRIINITSVVGEAGNPGQMN--YAAAKAGVAGMSRALAREIGSRNITVNCV 186 Query: 188 LPGATATDF 196 PG TD Sbjct: 187 APGFIDTDM 195 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.131 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 824,046 Number of extensions: 33606 Number of successful extensions: 261 Number of sequences better than 1.0e-08: 42 Number of HSP's gapped: 220 Number of HSP's successfully gapped: 42 Length of query: 272 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 188 Effective length of database: 1,222,130 Effective search space: 229760440 Effective search space used: 229760440 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)