BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0053.p (252 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313914|ref|YP_003778006.1| oxidoreductase [Herbaspirillum ... 443 e-126 gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogena... 86 4e-18 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 81 8e-17 gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogena... 78 9e-16 gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogena... 73 2e-14 >gi|300313914|ref|YP_003778006.1| oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 443 bits (1140), Expect = e-126, Method: Compositional matrix adjust. Identities = 216/252 (85%), Positives = 236/252 (93%) Query: 1 MKLTDNTLFITGGTSGIGRALAEAFHRLGNQVIIGGRRKALLDEVTAANPGMQAIEFDVS 60 M+LTDNT+FITGG SGIGR LAEAFH+LGN VIIGGRR+ALLDEV AANPGMQAI FDV+ Sbjct: 1 MQLTDNTIFITGGASGIGRGLAEAFHKLGNTVIIGGRRQALLDEVVAANPGMQAIAFDVA 60 Query: 61 SLASIEAAAATLKARFPTLNVLINNAGIMPFDDAGDTIDDQVSRSILDTNLLGPIRLTSA 120 ASI+AAAATLKAR+P LNVL+NNAGIMPFDDA IDD+V+R ILDTNLLGPIRLTSA Sbjct: 61 DPASIDAAAATLKARYPGLNVLVNNAGIMPFDDASGHIDDEVARRILDTNLLGPIRLTSA 120 Query: 121 LIDLLKAQPRATIIHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRDTSVRVQEIA 180 LIDLLK QPRATI+HNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLR+TSVRVQEIA Sbjct: 121 LIDLLKQQPRATILHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRETSVRVQEIA 180 Query: 181 PPWVDTDLVKKSGDPRAMPLEAFIAETMAKLAGDEEEIIVESVKPLRDNPGANEHALVNG 240 PPWVDTDLVKKSGDPRAMPL+AFI ETMA+LA ++EEIIV++VKPLRDNPGA EHALVNG Sbjct: 181 PPWVDTDLVKKSGDPRAMPLDAFITETMARLASEDEEIIVDAVKPLRDNPGAKEHALVNG 240 Query: 241 FNAALIANPIPV 252 FNAAL+A+PIPV Sbjct: 241 FNAALMAHPIPV 252 >gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 85.5 bits (210), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 59/176 (33%), Positives = 92/176 (52%), Gaps = 2/176 (1%) Query: 8 LFITGGTSGIGRALAEAFHRLGNQVIIGGRRKALLDEVTA--ANPGMQAIEFDVSSLASI 65 + ITG T+G G A+A F G++VI GRRK LD++ + + DV+S +SI Sbjct: 3 VLITGATAGFGAAMARKFAGNGHKVIAAGRRKERLDDLVKELGADKVLPLVLDVTSKSSI 62 Query: 66 EAAAATLKARFPTLNVLINNAGIMPFDDAGDTIDDQVSRSILDTNLLGPIRLTSALIDLL 125 E A+L + ++VLINNAG+ + +++DTN+ G + +T AL+ + Sbjct: 63 ETGLASLTGAWKEVDVLINNAGLALGLGPAHEVALSDWETMIDTNVKGLVTITHALLPDM 122 Query: 126 KAQPRATIIHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRDTSVRVQEIAP 181 + R TII+ SV P VY A+KA + + ++ R L T VR +AP Sbjct: 123 VKRGRGTIINLGSVAGAYPYPGGNVYGATKAFVDQFTLNLRADLVGTGVRATNLAP 178 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 80.9 bits (198), Expect = 8e-17, Method: Compositional matrix adjust. Identities = 63/202 (31%), Positives = 100/202 (49%), Gaps = 12/202 (5%) Query: 3 LTDNTLFITGGTSGIGRALAEAFHRLGNQVIIGGRRKALLDEVTAANPGMQAIEFDVSSL 62 L T +TGGT GIG A+A LG +VI G +E + + +E +V+S Sbjct: 8 LKGKTALVTGGTQGIGAAVARQLVALGARVIAAGLPPFAHEEFAGSE--VTLVEMNVASE 65 Query: 63 ASIEAAAATLKARFPTLNVLINNAGIMPFDDAGDTIDDQVSRSILDTNLLGPIRLTSALI 122 S+ AA A L L++++N AGI+ G ++ +V ++ NL G +R+ +A Sbjct: 66 ESVRAALAGLT----QLDIVVNCAGII---KRGAELETEVFEQVIAVNLTGTMRVCAAAR 118 Query: 123 DLLKAQPRATIIHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRDTSVRVQEIAPP 182 +LL+ Q R I++ S+L++ + YSASK + S +RV +AP Sbjct: 119 ELLR-QSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPG 177 Query: 183 WVDTDLVKK-SGDP-RAMPLEA 202 W+ T L + DP RA P+ A Sbjct: 178 WIATPLTQALQDDPVRAGPILA 199 >gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 248 Score = 77.8 bits (190), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 62/197 (31%), Positives = 98/197 (49%), Gaps = 7/197 (3%) Query: 3 LTDNTLFITGGTSGIGRALAEAFHRLGNQVIIGGRR----KALLDEVTAANPGMQAIEFD 58 + D + ITG +SG+G A LG ++++G RR + L+ ++TAA A+ D Sbjct: 5 IKDKVVVITGASSGLGETTARHLASLGAKLVLGARRTERLEKLVADITAAGGQAIAVTTD 64 Query: 59 VSSLASIEAAAATLKARFPTLNVLINNAGIMPFDDAGDTIDDQVSRSILDTNLLGPIRLT 118 V+ +EA A + F ++VL+NNAGIMP ++ R ++D N+ G + Sbjct: 65 VARRDDVEALVAKGEQHFGRIDVLVNNAGIMPLAPMAKLKVEEWDR-MIDVNVKGVLYGV 123 Query: 119 SALIDLLKAQPRATIIHNTSVLAYVPLA-VTAVYSASKAALHSYAMSQRFMLRDTSVRVQ 177 +A + AQ II+ +SV A + VYSA+K A+ + R D VR Sbjct: 124 AAALPRFAAQSSGHIINVSSVAGIKVFAGMGTVYSATKFAVRALTEGLRTEAPD-GVRTT 182 Query: 178 EIAPPWVDTDLVKKSGD 194 I+P VD++L S D Sbjct: 183 IISPGAVDSELKTHSSD 199 >gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 73.2 bits (178), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 62/178 (34%), Positives = 97/178 (54%), Gaps = 11/178 (6%) Query: 6 NTLFITGGTSGIGRALAEAFHRLGNQVIIGGR--RKALLDEVTAANPGMQAIEFDVSSLA 63 T+FITG +SG G +A F + +VI R R LL A+P ++ + DV+ Sbjct: 2 KTIFITGCSSGFGLEIARYFLQRDWKVIATMRTPRHDLLP----ASPHLRILPLDVADAH 57 Query: 64 SIEAAAATLKARFPTLNVLINNAGIMPFDDAGDTIDDQVSRSILDTNLLGPIRLTSALID 123 SI+AA ++A P ++VL+NNAGI QV R + +TN LG I +T A I Sbjct: 58 SIQAA---VEAAGP-VDVLVNNAGIGNLGALEAQPMAQV-RELFETNTLGSIAMTQAFIP 112 Query: 124 LLKAQPRATIIHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRDTSVRVQEIAP 181 ++A+ + +I+ TS + L + AVY+ASKAA++++ S L V+V+ + P Sbjct: 113 QMRARGQGLVINVTSSVTLRALPLLAVYTASKAAINAFTESLALELAPLGVQVRLVLP 170 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 752,471 Number of extensions: 29031 Number of successful extensions: 213 Number of sequences better than 1.0e-08: 34 Number of HSP's gapped: 184 Number of HSP's successfully gapped: 34 Length of query: 252 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 169 Effective length of database: 1,226,865 Effective search space: 207340185 Effective search space used: 207340185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)