BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0052.p (407 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux ... 563 e-162 gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembr... 123 3e-29 gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herb... 122 8e-29 gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herb... 79 8e-16 >gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 563 bits (1452), Expect = e-162, Method: Compositional matrix adjust. Identities = 308/404 (76%), Positives = 345/404 (85%), Gaps = 1/404 (0%) Query: 1 MTSTPLEPAPAPAPSASPSPSGSLLTAGFLALGTFAIGTEGFMIAPLLPRMAADFGMSVS 60 M PL P+ AP+ ASP+ +GSLLTAGFLALGTFAIGTEGFMIAPLLPRMAADFGMSV Sbjct: 1 MNPPPLGPS-APSACASPTQAGSLLTAGFLALGTFAIGTEGFMIAPLLPRMAADFGMSVP 59 Query: 61 TVASLVIVFTLVLAISSPIATVLSAHINRRRVLMGAMGVFTAANLVAALSSGFGGLLASR 120 VASLV++FTLVLA+SSPI+TVLSA +NRRRVL+ AM FTAANL+AA SS F GLL +R Sbjct: 60 MVASLVVIFTLVLALSSPISTVLSAAMNRRRVLIAAMTAFTAANLLAACSSSFTGLLVAR 119 Query: 121 LLMAVAAGLYTPNASALAGAIVKPEQRGRALAIVSGGMTIAIALGLPLGSVVGHAFGWRA 180 LLMAVAAGLYTPNASALAGA+V P QRGRALAIVSGGMT ALGLPLG+VVGHAFGWRA Sbjct: 120 LLMAVAAGLYTPNASALAGAMVAPGQRGRALAIVSGGMTSPSALGLPLGAVVGHAFGWRA 179 Query: 181 TFGAVAVMGAVAVAGIWLGVGRDAGGQMRVAGLRERLAVVGQPLIRRLLGVTLFWSVGAY 240 TF AV+ MGAVA+AGI LGV R AG + VAGLRER+AV+GQP ++RLL VTL WSVGAY Sbjct: 180 TFLAVSAMGAVALAGILLGVPRQAGAHLHVAGLRERMAVIGQPQVQRLLAVTLLWSVGAY 239 Query: 241 AAYPYVAPYLAHTLAFADRGIGLTVSLWGAAAAAGVTVGGHLNDRFGSPRVVRLSLLLLM 300 AAYPY+APYL+H L+F + I LTVSLWGAAAA GV+VGG LNDR+GS RVVR+SLLLLM Sbjct: 240 AAYPYIAPYLSHVLSFGESRISLTVSLWGAAAAVGVSVGGTLNDRYGSARVVRVSLLLLM 299 Query: 301 LAFWSLSAATLLSAQAALIPVLVAVVAWGFAVWAFFPAQMARLIASGAAAQAPVALSLNT 360 L+FW+L+AAT L AL PVL+AVV WGF+VWAFFPAQMARLI +GA AQAPVALSLNT Sbjct: 300 LSFWTLAAATALPPPLALGPVLLAVVVWGFSVWAFFPAQMARLIGAGAPAQAPVALSLNT 359 Query: 361 STMYLGFSLGSAAGAGILAQGWMAGIGLFAGTCALAGLLLEQRL 404 ST+YLGFSLGSAAGAGIL G + GIGLFAG CAL LLE+RL Sbjct: 360 STLYLGFSLGSAAGAGILGSGAIWGIGLFAGACALVAWLLERRL 403 >gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 395 Score = 123 bits (309), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 84/256 (32%), Positives = 141/256 (55%), Gaps = 2/256 (0%) Query: 30 LALGTFAIGTEGFMIAPLLPRMAADFGMSVSTVASLVIVFTLVLAISSPIATVLSAHINR 89 L + FAIGT F+I LLP +AA+ G+S+S+ LV + L + + +P+ T + + R Sbjct: 8 LTISAFAIGTTEFVIMGLLPEVAANLGVSLSSAGLLVTGYALGVFVGAPLLTATTGRLPR 67 Query: 90 RRVLMGAMGVFTAANLVAALSSGFGGLLASRLLMAVAAGLYTPNASALAGAIVKPEQRGR 149 + +L+ M VF NL A++ + L+ R++ + A G + S +A ++V E++ Sbjct: 68 KTLLVALMVVFVVGNLACAIAPDYTTLMIGRVVASFAHGAFFGVGSVVATSLVPKEKQAS 127 Query: 150 ALAIVSGGMTIAIALGLPLGSVVGHAFGWRATFGAVAVMGAVAVAGIWLGVGRDAGGQMR 209 A+A++ G+T+A LG+P G+ +G ++GWRATF AV V+G +A+A + V R GG+ Sbjct: 128 AIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPRSQGGEET 187 Query: 210 VAGLRERLAVVGQPLIRRLLGVTLFWSVGAYAAYPYVAPYLAHTLAFADRGIGLTVSLWG 269 G R+ + Q ++ L+ V F G +AA+ Y+AP L F + + L+G Sbjct: 188 DFGKEVRVLMRPQVMLGLLMTVLGFG--GVFAAFTYIAPILTDLSGFPASAVSPILLLFG 245 Query: 270 AAAAAGVTVGGHLNDR 285 G GG L D+ Sbjct: 246 LGLVIGNLAGGKLADK 261 >gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 385 Score = 122 bits (305), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 110/365 (30%), Positives = 184/365 (50%), Gaps = 25/365 (6%) Query: 30 LALGTFAIGTEGFMIAPLLPRMAADFGMSVSTVASLVIVFTLVLAISSPIATVLSAHINR 89 LA+ F IGT F+I LLP +A D G++ LV + L +A +PI +L+A R Sbjct: 8 LAVAAFGIGTTEFVIMGLLPDVARDLGVTAPAAGMLVSGYALGVAAGAPILAILTAKWPR 67 Query: 90 RRVLMGAMGVFTAANLVAALSSGFGGLLASRLLMAVAAGLYTPNASALAGAIVKPEQRGR 149 ++ L+G M +F N++ AL+ + L+ +R++ A + S +A +V P++R + Sbjct: 68 KQALVGLMCLFILGNVLCALAPNYVFLMVARVVTAFCHAAFFGIGSVVAAELVAPQKRAQ 127 Query: 150 ALAIVSGGMTIAIALGLPLGSVVGHAFGWRATFGAVAVMGAVAVAGIWLGVGRDAGGQMR 209 A+A++ G+T+A LG+PLG+ GHA GWR+TFGAV +G +A +W + + Q Sbjct: 128 AVALMFTGLTVANILGVPLGTAFGHAAGWRSTFGAVTAIGVLAAIMLWTMLPKKIPMQ-- 185 Query: 210 VAG--LRERLAVVGQPLIRRLLGVTLFWSVGAYAAYPYVAPYLAHTLAFADRGIGLTVSL 267 AG +E +++ + LL TL S+ ++ Y+A L F + + L Sbjct: 186 -AGNIFQEFRSIIDTRVQLSLLTSTLA-SISMFSVITYIAYILLDVTGFTTQQESWVLLL 243 Query: 268 WGAAAAAGVTVGGHLNDRFGSPRVVRLSLLL-LMLAFWSLSAATLLSAQAALIPVLVAVV 326 +GA G VGG L D P + + L + ++L +S+++ P L + Sbjct: 244 FGAGITVGGLVGGKLADWKLMPVMCGVFLCIAVVLGLFSITSHYKW-------PTLATMF 296 Query: 327 AWGFAVWAFFPAQMARLIASGAAAQAPVALSLNTSTMYLGFSLGSAAGAGILAQGWMAGI 386 AWG +A P R++ + A +A +LN F+LG+A GA W+ G+ Sbjct: 297 AWGAFAFAVVPGAQIRVLDTARGAPN-LASTLNQG----AFNLGNATGA------WVGGL 345 Query: 387 GLFAG 391 + AG Sbjct: 346 AISAG 350 >gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 422 Score = 78.6 bits (192), Expect = 8e-16, Method: Compositional matrix adjust. Identities = 63/223 (28%), Positives = 117/223 (52%), Gaps = 2/223 (0%) Query: 30 LALGTFAIGTEGFMIAPLLPRMAADFGMSVSTVASLVIVFTLVLAISSPIATVLSAHINR 89 LALG F++ T F+ A LL +A+D +S V +V A+++P +L+ +I+R Sbjct: 42 LALGVFSLVTAEFLPASLLTAIASDLHVSDGAAGQAVTATAIVGAVAAPSIPLLTRNIDR 101 Query: 90 RRVLMGAMGVFTAANLVAALSSGFGGLLASRLLMAVAAGLYTPNASALAGAIVKPEQRGR 149 +RV++ + +N++A + LL +R+L+ VA G + A+ALA +V R Sbjct: 102 KRVVLMLSLLLLVSNILAVTAPNLPVLLVARVLLGVALGGFWSMAAALAMRLVPDHLFAR 161 Query: 150 ALAIVSGGMTIAIALGLPLGSVVGHAFGWRATFGAVAVMGAVAVAGIWLGVGRDAGGQMR 209 A++++ G+++A P+G+ +G+ +GWR+ F A V+ VA+A W+ + R Sbjct: 162 AMSLILTGVSVATVCAAPIGAYMGNLWGWRSAFVAAGVVSLVAIAIQWIAIPRLPPKDH- 220 Query: 210 VAGLRERLAVVGQPLIRRLLGVTLFWSVGAYAAYPYVAPYLAH 252 L+ ++G+ +R L L G +A + Y+ P + Sbjct: 221 -PSLKVLAELLGRKDVRVALLAVLLVVSGHFAGFTYIRPLMEQ 262 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.136 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 930,162 Number of extensions: 34478 Number of successful extensions: 386 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 382 Number of HSP's successfully gapped: 19 Length of query: 407 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 320 Effective length of database: 1,207,925 Effective search space: 386536000 Effective search space used: 386536000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)