BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0041.p (312 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313922|ref|YP_003778014.1| LysR family transcription regul... 600 e-173 gi|300309921|ref|YP_003774013.1| LysR family transcription regul... 200 1e-52 gi|300311458|ref|YP_003775550.1| LysR family transcription regul... 195 5e-51 gi|300312196|ref|YP_003776288.1| LysR family transcription regul... 181 7e-47 gi|300309660|ref|YP_003773752.1| LysR family transcription regul... 180 2e-46 >gi|300313922|ref|YP_003778014.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 600 bits (1546), Expect = e-173, Method: Compositional matrix adjust. Identities = 294/312 (94%), Positives = 301/312 (96%), Gaps = 4/312 (1%) Query: 1 MDRLMAMQVFVTVVDGGSLSAAAEQLDLSRPVVSRYVAELEDWVGARLLHRTTRRLSLTP 60 MDRLMAMQVFVTVVD GSLSAAAEQLDLSRPVVSRYVAELEDWVGARLLHRTTRRLSLTP Sbjct: 1 MDRLMAMQVFVTVVDSGSLSAAAEQLDLSRPVVSRYVAELEDWVGARLLHRTTRRLSLTP 60 Query: 61 AGNELLPRCRQMLEYSEDMRHVLDTPDETPRGLLRITTSTSFAQTQLIQAVVEYGRRYPG 120 AGNELLPRCRQMLEYSEDMRH LD PDETPRGLLRIT STSFAQTQLIQAVVEYGRRYPG Sbjct: 61 AGNELLPRCRQMLEYSEDMRHALDAPDETPRGLLRITASTSFAQTQLIQAVVEYGRRYPG 120 Query: 121 VAVDLLALDRTVNLVEERIDLAIRMTNRLEPNLIARPLGVCHSVICATPDYLARHGMPRK 180 VAVDLLALDRTVNLVEERIDLAIRMTNRLEPNLIARPLGVC S+ICATPDYLARHG+PRK Sbjct: 121 VAVDLLALDRTVNLVEERIDLAIRMTNRLEPNLIARPLGVCRSIICATPDYLARHGLPRK 180 Query: 181 VEDLALHNCLTHSYVGRSLWQFDPKKKKARAGKGAAAAKMKTQTQSVAVGGTISANEVTV 240 VEDLALHNCLTHSYVGRSLWQFDPKKKKAR+G+GA+AAK QSVAVGG+ISANEVTV Sbjct: 181 VEDLALHNCLTHSYVGRSLWQFDPKKKKARSGRGASAAK----PQSVAVGGSISANEVTV 236 Query: 241 LLQAVLAGVGIGHMPAYTVAPLVASGQLVQLLPDYEPLQLGIYGVYASRKHMPATLRTML 300 LLQAVLAGVGIGHMPAYTVAPLVASGQLVQLLPDYEPLQLG+YGVYASRKHMPATLRTML Sbjct: 237 LLQAVLAGVGIGHMPAYTVAPLVASGQLVQLLPDYEPLQLGMYGVYASRKHMPATLRTML 296 Query: 301 DFLAQRFAGLPF 312 DFLAQRFAG PF Sbjct: 297 DFLAQRFAGQPF 308 >gi|300309921|ref|YP_003774013.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 299 Score = 200 bits (509), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 115/309 (37%), Positives = 166/309 (53%), Gaps = 17/309 (5%) Query: 1 MDRLMAMQVFVTVVDGGSLSAAAEQLDLSRPVVSRYVAELEDWVGARLLHRTTRRLSLTP 60 M++L ++ F+ V D GS AAE L +S+ VSR V ELE +G+RL+ RTTRRLSLT Sbjct: 1 MNKLREIECFIAVADLGSFVKAAEALGISKAAVSRTVLELEARLGSRLMQRTTRRLSLTE 60 Query: 61 AGNELLPRCRQMLEYSEDMRHVLDTPDETPRGLLRITTSTSFAQTQLIQAVVEYGRRYPG 120 AG L RC+Q++ E+ +L + P GLLRI +F L + R+P Sbjct: 61 AGALYLERCKQIVAALEEADDLLSSGSAKPTGLLRINAPLTFGVLHLAPLWPMFLERHPE 120 Query: 121 VAVDLLALDRTVNLVEERIDLAIRMTNRLEPNLIARPLGVCHSVICATPDYLARHGMPRK 180 +++D+ DR V++++E D+AIR+ + L+ R L +CA+P YL HG P Sbjct: 121 ISLDITLSDRIVDIIDEGYDMAIRIARLSDSTLVHRKLTSTSLHVCASPAYLDAHGTPAH 180 Query: 181 VEDLALHNCLTHSY-VGRSLWQFDPKKKKARAGKGAAAAKMKTQTQSVAVGGTISANEVT 239 +LA H +++SY + WQF+ + SV V + N Sbjct: 181 PHELAQHQTISYSYNQSKDEWQFNGPEGPV----------------SVRVSSRLHVNNGD 224 Query: 240 VLLQAVLAGVGIGHMPAYTVAPLVASGQLVQLLPDYEPLQLGIYGVYASRKHMPATLRTM 299 +QA L G+GI P + + + SGQLV +L DY +LGIY VY SR H+PA +R M Sbjct: 225 SCVQAALGGIGITRQPTFMIDQYLRSGQLVSILGDYGAPELGIYAVYPSRAHLPAKMRAM 284 Query: 300 LDFLAQRFA 308 LDFL + FA Sbjct: 285 LDFLTEAFA 293 >gi|300311458|ref|YP_003775550.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 303 Score = 195 bits (495), Expect = 5e-51, Method: Compositional matrix adjust. Identities = 112/310 (36%), Positives = 176/310 (56%), Gaps = 20/310 (6%) Query: 1 MDRLMAMQVFVTVVDGGSLSAAAEQLDLSRPVVSRYVAELEDWVGARLLHRTTRRLSLTP 60 MDRL +M+VF VV+ GS AAE LD+S V +R++A+LE +G RLL+R+TRRLSLT Sbjct: 1 MDRLQSMRVFAKVVEQGSFVRAAELLDISNAVATRFIADLEAHLGTRLLNRSTRRLSLTE 60 Query: 61 AGNELLPRCRQMLEYSEDMRHVLDTPDETPRGLLRITTSTSFAQTQLIQAVVEYGRRYPG 120 AG L R R +L +D ++ + P G L I + F Q+QL + + Y + YP Sbjct: 61 AGQAYLERVRMILAEVDDAEALVSLETKRPAGTLAIYCNAGFGQSQLGEMLSAYSQAYPE 120 Query: 121 VAVDLLALDRTVNLVEERIDLAIRMT-NRLEPNLIARPLGVCHSVICATPDYLARHGMPR 179 VA+D+ +R+++LVEE ID+ + + + ++I R LGV ++C +P Y+AR G P Sbjct: 121 VALDISLSERSIDLVEEGIDIGFFSSMQKFDASMIVRQLGVARVILCTSPAYIARQGAPE 180 Query: 180 KVEDLALHNCL--THSYVGRSLWQFDPKKKKARAGKGAAAAKMKTQTQSVAVGGTISANE 237 + EDL+ HNCL +H Y+ R W + V + + +N Sbjct: 181 QPEDLSRHNCLNFSHEYL-RHHWHVNTNGG----------------VLDVPIVSKVVSNS 223 Query: 238 VTVLLQAVLAGVGIGHMPAYTVAPLVASGQLVQLLPDYEPLQLGIYGVYASRKHMPATLR 297 +L LAG+GI P+Y++ + SG+LVQ+LP ++ Q+ + VY SR+ + A +R Sbjct: 224 GVLLRDLALAGMGIVLRPSYSLGDDLRSGRLVQVLPGFDMGQVIMSMVYPSRRLLSAKVR 283 Query: 298 TMLDFLAQRF 307 + +F++ RF Sbjct: 284 SFAEFISARF 293 >gi|300312196|ref|YP_003776288.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 181 bits (459), Expect = 7e-47, Method: Compositional matrix adjust. Identities = 108/314 (34%), Positives = 165/314 (52%), Gaps = 17/314 (5%) Query: 1 MDRLMAMQVFVTVVDGGSLSAAAEQLDLSRPVVSRYVAELEDWVGARLLHRTTRRLSLTP 60 MD+L AMQ FV VV+ GS AA + S+P VSR V+ELE ++G RLLHRTTRRLSLT Sbjct: 1 MDKLQAMQTFVAVVESGSFVAAVDVTGQSKPAVSRQVSELEQFLGIRLLHRTTRRLSLTD 60 Query: 61 AGNELLPRCRQMLEYSEDMRHVLDTPDETPRGLLRITTSTSFAQTQLIQAVVEYGRRYPG 120 G RC+++LE +D+ + + G LRI F QL ++ ++P Sbjct: 61 EGRVYFARCKELLETIQDVESEVGLNSDQACGRLRIGAPQDFGVEQLAPLWGKFTEQHPL 120 Query: 121 VAVDLLALDRTVNLVEERIDLAIRMTNRLEPNLIARPLGVCHSVICATPDYLARHGMPRK 180 + +++ DRT++L+EE D+AIR++ + ++ARPL + CA P++LARHG P Sbjct: 121 ITLNITLSDRTIDLLEEGFDMAIRISALGDSRMVARPLAPVRMIACAAPEFLARHGTPGT 180 Query: 181 VEDLALHNCLTHSY-VGRSLWQFDPKKKKARAGKGAAAAKMKTQTQSVAVGGTISANEVT 239 +L + +++SY G W F + T V V T+ N Sbjct: 181 PAELRDYPFISYSYRAGGDDWSF---------------RHVHGDTCQVRVRSTVYVNNGA 225 Query: 240 VLLQAVLAGVGIGHMPAYTVAPLVASGQLVQLLPDYEP-LQLGIYGVYASRKHMPATLRT 298 +AG GI P + V + +GQLV+L + QL ++ +Y +R H+P ++ Sbjct: 226 TCRAMAIAGQGIAIQPDFIVNAAIQAGQLVELFEGWSTGEQLVVHTLYPTRTHLPPKVQR 285 Query: 299 MLDFLAQRFAGLPF 312 M DFL ++F P+ Sbjct: 286 MRDFLIEQFRVPPW 299 >gi|300309660|ref|YP_003773752.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 337 Score = 180 bits (456), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 104/315 (33%), Positives = 163/315 (51%), Gaps = 19/315 (6%) Query: 1 MDRLMAMQVFVTVVDGGSLSAAAEQLDLSRPVVSRYVAELEDWVGARLLHRTTRRLSLTP 60 MDR AMQVF VVD S + AA+ L+L R V+ V LE + RLL+RTTRR+SLTP Sbjct: 1 MDRFQAMQVFTAVVDANSFTRAADNLNLPRTTVTTLVQGLESMLQVRLLNRTTRRISLTP 60 Query: 61 AGNELLPRCRQMLEYSEDMRHVLDTPDETPRGLLRITTSTSFAQTQLIQAVVEYGRRYPG 120 G RC ++L ++ P+G LRI S + L+ + ++ RYP Sbjct: 61 DGAAYYERCVRILADLDETEQSFRNVTRGPQGRLRIDVPASVGRLMLLPNLCDFYTRYPD 120 Query: 121 VAVDLLALDRTVNLVEERIDLAIRMTNRLEPNLIARPLGVCHSVICATPDYLARHGMPRK 180 + + + DR V+LV+E +D IR+ + +++AR +G +V CA P YL RHG+P Sbjct: 121 IELMMGMGDRPVDLVQEAVDCVIRIGELQDSSMVARRIGTFQTVTCAAPAYLERHGVPHT 180 Query: 181 VEDLALHNCLTH--SYVGRSL-WQFDPKKKKARAGKGAAAAKMKTQTQSVAVGGTISANE 237 +EDL H + + S GR++ W F ++ T+ V + G ++ NE Sbjct: 181 IEDLQQHKAVHYFSSRTGRTIDWDF----------------VVEGVTREVKMNGKLAVNE 224 Query: 238 VTVLLQAVLAGVGIGHMPAYTVAPLVASGQLVQLLPDYEPLQLGIYGVYASRKHMPATLR 297 ++ L G G+ P Y V + G+LV++L ++P + I VY +H+ +R Sbjct: 225 ADAYVECALQGFGMVQAPLYMVTRHIDEGRLVEVLSQWKPAPMPISVVYLHNRHLSPKVR 284 Query: 298 TMLDFLAQRFAGLPF 312 +D++A+ FA P Sbjct: 285 AFVDWVAELFATCPL 299 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 904,086 Number of extensions: 35038 Number of successful extensions: 296 Number of sequences better than 1.0e-08: 57 Number of HSP's gapped: 251 Number of HSP's successfully gapped: 57 Length of query: 312 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 227 Effective length of database: 1,217,395 Effective search space: 276348665 Effective search space used: 276348665 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)