BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0033.p (311 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311233|ref|YP_003775325.1| LysR family transcription regul... 267 1e-72 gi|300313187|ref|YP_003777279.1| transcription regulator protein... 167 7e-43 gi|300309921|ref|YP_003774013.1| LysR family transcription regul... 164 1e-41 gi|300313922|ref|YP_003778014.1| LysR family transcription regul... 160 9e-41 gi|300311443|ref|YP_003775535.1| LysR family transcription regul... 154 9e-39 >gi|300311233|ref|YP_003775325.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 315 Score = 267 bits (682), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 134/306 (43%), Positives = 195/306 (63%) Query: 1 MRTNELAEVLHELVTFVQVVDAGSFSAAARQQGMTPSAVSRQVARLEKMMGVALFQRTTR 60 M TN+ +L+E+ F +VV+ GSFS AARQ G TPSA+SR +ARLEK +G L QRTTR Sbjct: 1 MSTNQSIALLNEMAVFAKVVETGSFSEAARQLGATPSAISRAIARLEKALGTQLLQRTTR 60 Query: 61 QLRLTEAGLDVLEHGRKMVAAARATLQAAEGHAGAPKGLVRMSAPKAVSRHLLQPVLLQF 120 +LRL+E+G D+ R M+ AA+A L + P+G VR+S PKAV L+ P + F Sbjct: 61 KLRLSESGQDIHARCRDMLNAAQAVLAVSGAFDAEPQGQVRVSVPKAVGHALVHPHVPDF 120 Query: 121 LARYPDVDVHLMVVDRLVDPLREGVDLVIRLTDTPPPGMVARPLLPVSQCLLASPDYLAS 180 LA +P VDV L + DR +D + + VDL IR+TD PPPG++ R L+ + L A+P YLA+ Sbjct: 121 LAAHPKVDVLLRLEDRYMDLIDDEVDLAIRITDHPPPGLMGRRLMRIDHLLCATPHYLAT 180 Query: 181 RGAIHHPEDLMSHSCLTLGEEERDNRWRFLSGTQAAEIIVTGRCAVNHSEMRLAAIEAGL 240 G HP+ L HSC+ LGE D RWRF ++ + V GR A NH+ +RL A+ L Sbjct: 181 HGTPAHPQALKEHSCIYLGESPADARWRFRRQGKSVNVEVRGRYAANHTGVRLDAVLRHL 240 Query: 241 GVGCVPDFVAKESLAAGRVVRLLPEWTVDSNYQGVAYLLFPASRHIAPKMRVLIDYLVEA 300 G+ +P F+A+ +L G +V++LP+W+ ++Y G + L+PA+RH+ P++ + +L + Sbjct: 241 GIASLPYFIARPALEEGSLVQVLPQWSFRTHYCGELWALYPATRHLPPRLGAFVQFLAQR 300 Query: 301 LQARAT 306 L T Sbjct: 301 LAQEPT 306 >gi|300313187|ref|YP_003777279.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 167 bits (424), Expect = 7e-43, Method: Compositional matrix adjust. Identities = 110/296 (37%), Positives = 155/296 (52%), Gaps = 8/296 (2%) Query: 8 EVLHELVTFVQVVDAGSFSAAARQQGMTPSAVSRQVARLEKMMGVALFQRTTRQLRLTEA 67 E + + FV AGSFSAAARQ G+T +AV + VARLE +GV LF R+TR+L LTEA Sbjct: 2 ETMTSIECFVAAAHAGSFSAAARQLGLTSAAVGKNVARLESSLGVRLFHRSTRKLSLTEA 61 Query: 68 GLDVLEHGRKMVAAARATLQAAEGHAGAPKGLVRMSAPKAVSRHLLQPVLLQFLARYPDV 127 G L+ + A R+ + G P G +++S R + P+L +FL RYP + Sbjct: 62 GERFLQEVGGGLQAIRSAVANLASAGGQPAGTLKVSMGTGFGRQYIVPLLGEFLGRYPGI 121 Query: 128 DVHLMVVDRLVDPLREGVDLVIRLTDTPPPGMVARPLLPVSQCLLASPDYLASRGAIHHP 187 +R VD + EG D I PG+VAR L P + LLASP YL + +H P Sbjct: 122 QPDWHFENRPVDLISEGFDAAIAGGTELSPGVVARELAPAHRVLLASPAYLDAHPPLHSP 181 Query: 188 EDLMSHSCLTLGEEE----RDNRWRFLSGTQAAEIIVTGRCAVNHSEMRLAAIEAGLGVG 243 DL H+ + + + R R SG Q A + + R ++ + EAGLG+ Sbjct: 182 ADLAEHAGILIRSPQTGRVRPFSLRDRSGQQ-APVTLQQRMTMSDANAACEVAEAGLGIT 240 Query: 244 CVPDFVAKESLAAGRVVRLLPEWTVDSNYQGVAYLLFPASRHIAPKMRVLIDYLVE 299 V A L G +VR+LP+W VD+ G+ L F A+R + K RV +D++VE Sbjct: 241 LVAMPHALPYLEGGTLVRVLPDWYVDT---GMLSLYFTAARLLPAKTRVFVDFVVE 293 >gi|300309921|ref|YP_003774013.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 299 Score = 164 bits (414), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 97/292 (33%), Positives = 154/292 (52%), Gaps = 3/292 (1%) Query: 10 LHELVTFVQVVDAGSFSAAARQQGMTPSAVSRQVARLEKMMGVALFQRTTRQLRLTEAGL 69 L E+ F+ V D GSF AA G++ +AVSR V LE +G L QRTTR+L LTEAG Sbjct: 4 LREIECFIAVADLGSFVKAAEALGISKAAVSRTVLELEARLGSRLMQRTTRRLSLTEAGA 63 Query: 70 DVLEHGRKMVAAARATLQAAEGHAGAPKGLVRMSAPKAVSRHLLQPVLLQFLARYPDVDV 129 LE +++VAA + P GL+R++AP L P+ FL R+P++ + Sbjct: 64 LYLERCKQIVAALEEADDLLSSGSAKPTGLLRINAPLTFGVLHLAPLWPMFLERHPEISL 123 Query: 130 HLMVVDRLVDPLREGVDLVIRLTDTPPPGMVARPLLPVSQCLLASPDYLASRGAIHHPED 189 + + DR+VD + EG D+ IR+ +V R L S + ASP YL + G HP + Sbjct: 124 DITLSDRIVDIIDEGYDMAIRIARLSDSTLVHRKLTSTSLHVCASPAYLDAHGTPAHPHE 183 Query: 190 LMSHSCLTLGEEERDNRWRFLSGTQAAEIIVTGRCAVNHSEMRLAAIEAGLGVGCVPDFV 249 L H ++ + + W+F + V+ R VN+ + + A G+G+ P F+ Sbjct: 184 LAQHQTISYSYNQSKDEWQFNGPEGPVSVRVSSRLHVNNGDSCVQAALGGIGITRQPTFM 243 Query: 250 AKESLAAGRVVRLLPEWTVDSNYQGVAYLLFPASRHIAPKMRVLIDYLVEAL 301 + L +G++V +L ++ G+ Y ++P+ H+ KMR ++D+L EA Sbjct: 244 IDQYLRSGQLVSILGDYGAPE--LGI-YAVYPSRAHLPAKMRAMLDFLTEAF 292 >gi|300313922|ref|YP_003778014.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 160 bits (406), Expect = 9e-41, Method: Compositional matrix adjust. Identities = 101/302 (33%), Positives = 164/302 (54%), Gaps = 16/302 (5%) Query: 10 LHELVTFVQVVDAGSFSAAARQQGMTPSAVSRQVARLEKMMGVALFQRTTRQLRLTEAGL 69 L + FV VVD+GS SAAA Q ++ VSR VA LE +G L RTTR+L LT AG Sbjct: 4 LMAMQVFVTVVDSGSLSAAAEQLDLSRPVVSRYVAELEDWVGARLLHRTTRRLSLTPAGN 63 Query: 70 DVLEHGRKMVAAARATLQAAEGHAGAPKGLVRMSAPKAVSRHLLQPVLLQFLARYPDVDV 129 ++L R+M+ + A + P+GL+R++A + ++ L ++++ RYP V V Sbjct: 64 ELLPRCRQMLEYSEDMRHALDAPDETPRGLLRITASTSFAQTQLIQAVVEYGRRYPGVAV 123 Query: 130 HLMVVDRLVDPLREGVDLVIRLTDTPPPGMVARPLLPVSQCLLASPDYLASRGAIHHPED 189 L+ +DR V+ + E +DL IR+T+ P ++ARPL + A+PDYLA G ED Sbjct: 124 DLLALDRTVNLVEERIDLAIRMTNRLEPNLIARPLGVCRSIICATPDYLARHGLPRKVED 183 Query: 190 LMSHSCLTLGEEERDNRWRF------------LSGTQAAEIIVTGRCAVNHSEMRLAAIE 237 L H+CLT R + W+F S + + V G + N + L A+ Sbjct: 184 LALHNCLTHSYVGR-SLWQFDPKKKKARSGRGASAAKPQSVAVGGSISANEVTVLLQAVL 242 Query: 238 AGLGVGCVPDFVAKESLAAGRVVRLLPEWTVDSNYQGVAYLLFPASRHIAPKMRVLIDYL 297 AG+G+G +P + +A+G++V+LLP++ Q Y ++ + +H+ +R ++D+L Sbjct: 243 AGVGIGHMPAYTVAPLVASGQLVQLLPDY---EPLQLGMYGVYASRKHMPATLRTMLDFL 299 Query: 298 VE 299 + Sbjct: 300 AQ 301 >gi|300311443|ref|YP_003775535.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 324 Score = 154 bits (389), Expect = 9e-39, Method: Compositional matrix adjust. Identities = 101/303 (33%), Positives = 160/303 (52%), Gaps = 11/303 (3%) Query: 10 LHELVTFVQVVDAGSFSAAARQQGMTPSAVSRQVARLEKMMGVALFQRTTRQLRLTEAGL 69 L+++ FV VV AGSF+AA R+ M P+ +SR++ LE MGV L QR+TRQL +T AG Sbjct: 4 LNDIWLFVHVVRAGSFAAAGRRLSMPPNTISRRLQSLEAAMGVRLLQRSTRQLNMTAAGR 63 Query: 70 DVLEH---GRKMVAAARATLQAAEGHAGAPKGLVRMSAPKAVSRHLLQPVLLQFLARYPD 126 + E G + + A A+L ++G P G +R++AP + + +F+ YP Sbjct: 64 EFFERCAPGLEDIEHASASLTESQGE---PAGSLRVAAPVDFFDNFSIEWMHEFMHLYPK 120 Query: 127 VDVHLMVVDRLVDPLREGVDLVIRLTDTPPPGMVARPLLPVSQCLLASPDYLASRGAIHH 186 V + ++ D D + EG+DL R P +VAR L+ L+ASP YL +G Sbjct: 121 VQLEFVLNDGRADLIAEGIDLAFRGGVLPDSSLVARKLVQSHLALIASPRYLERQGTPQS 180 Query: 187 PEDLMSHSCLTLGEEERDNRWRFLSGTQAAEII-VTGRCAVNHSEMRLAAIEAGLGVGCV 245 DL +H+CL + + W+ L G E + VT R VN ++ +L A AGLG+ + Sbjct: 181 LTDLTAHACLATSQAPQHTVWK-LEGPHGPESVKVTPRLCVNTAQGQLRAARAGLGIALL 239 Query: 246 PDFVAKESLAAGRVVRLLPEWTVDSNYQGVAYLLFPASRHIAPKMRVLIDYLVEALQARA 305 P +A E L G +V +LP++ D+ G Y ++ R + + LI++ + L+ Sbjct: 240 PTMLASEDLRNGTLVNILPDYQRDT--MGF-YAVYVHRRQLPSAVSALIEFFADKLERGI 296 Query: 306 TDQ 308 D+ Sbjct: 297 ADK 299 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.134 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 969,425 Number of extensions: 41649 Number of successful extensions: 306 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 239 Number of HSP's successfully gapped: 70 Length of query: 311 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 226 Effective length of database: 1,217,395 Effective search space: 275131270 Effective search space used: 275131270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)