BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0028.p (397 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC t... 808 0.0 gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC t... 100 2e-22 gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC t... 100 2e-22 gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC t... 100 3e-22 gi|300314071|ref|YP_003778163.1| amino acid ABC transporter peri... 89 4e-19 >gi|300313931|ref|YP_003778023.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 808 bits (2086), Expect = 0.0, Method: Compositional matrix adjust. Identities = 388/397 (97%), Positives = 396/397 (99%) Query: 1 MISRRNFLKLSALAAPGALSYHDVFAQAESIAFGCPVPMSGPFAANGKFADMGMKLALQK 60 MISRR+FLKLSALAAPGALSYHDVFAQAESIAFGCPVPMSGPFAANGKFAD+GMKLALQK Sbjct: 1 MISRRHFLKLSALAAPGALSYHDVFAQAESIAFGCPVPMSGPFAANGKFADLGMKLALQK 60 Query: 61 YGKVLGKSTSYVTLDTEGKPATAVRKMQETIEKGTRFFAGGILSGEALAMGKEADKAGAV 120 YGKVLGKSTSYV LDTEGKPATAVRKMQE+IEKGTRFFAGGILSGEALAMGKEADKAGAV Sbjct: 61 YGKVLGKSTSYVNLDTEGKPATAVRKMQESIEKGTRFFAGGILSGEALAMGKEADKAGAV 120 Query: 121 FITTAGADEITGSDCNRATFRWSVPTFGAIEQTVRPLLEKLPNAKRWYTITPQYVFGEGL 180 FITTAGADEITGSDCNRATFRWSVPTFGAIEQTVRPLLEK+PNAKRWYTITPQYVFGEGL Sbjct: 121 FITTAGADEITGSDCNRATFRWSVPTFGAIEQTVRPLLEKMPNAKRWYTITPQYVFGEGL 180 Query: 181 LSAAKNIFKEKGIEHVGNSYHSLTEKEFSGYLTNAMAAKPDVLLLLNFGSQSSDTLRQAV 240 LSAAKNIFKEKGIEHVGNSYHSLTEKEFSGYLTNA+AAKPDVLLLLNFGSQSSDTLRQAV Sbjct: 181 LSAAKNIFKEKGIEHVGNSYHSLTEKEFSGYLTNAIAAKPDVLLLLNFGSQSSDTLRQAV 240 Query: 241 SFGMKNNCTILMAWASGLEQFETLGADICDGVYFGAQYWHAIDSPFNKEFVKLVNDSLKI 300 SFGMKNNCTILMAWASGLEQFETLGADICDGVYFGAQYWHAID+PFNKEFVKLVND+LKI Sbjct: 241 SFGMKNNCTILMAWASGLEQFETLGADICDGVYFGAQYWHAIDAPFNKEFVKLVNDNLKI 300 Query: 301 DPNYSLAGSYICTKIILDAIIKANSTDPKAVIAAMEGMKYEGLTGTEEIRKGDHQVIKNY 360 DPNYSLAGSYICTKIILDAIIKANSTDPKAVIAAMEGMKYEGLTG+EEIRKGDHQVIKNY Sbjct: 301 DPNYSLAGSYICTKIILDAIIKANSTDPKAVIAAMEGMKYEGLTGSEEIRKGDHQVIKNY 360 Query: 361 YLLKGKAKAKMANKNDYADIVSSGKSFLPLEKTQCKL 397 YLLKGKAKAKMANKNDYADIVSSGKSFLPLEKTQCKL Sbjct: 361 YLLKGKAKAKMANKNDYADIVSSGKSFLPLEKTQCKL 397 >gi|300312372|ref|YP_003776464.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 100 bits (250), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 102/380 (26%), Positives = 159/380 (41%), Gaps = 15/380 (3%) Query: 28 AESIAFGCPVPMSGPFAA-NGKFADMGMKLALQKYGK--VLGKSTSYVTLDTEGKPATAV 84 A ++ G MSGP++A G+ + + K+A++ K G ++ D + KP V Sbjct: 33 ANTVKIGVLTDMSGPYSAMGGQGSVVAAKMAVEDCLKNECKGMKIEILSADNQNKPDVGV 92 Query: 85 RKMQETIEKG-TRFFAGGILSGEALAMGKE-ADKAGAVFITTAGADEITGSDCNRATFRW 142 + +E +++ A S ALA+ K DK G + +T ++C F W Sbjct: 93 TRAREWLDRDKVEAIADLTNSSVALAVQKLIKDKGGIAMYSGPATGALTTAECAPNGFHW 152 Query: 143 SVPTFGAIEQTVRPLLEKLPNAKRWYTITPQYVFGEGLLSAAKNIFKEKGIEHVGNSYHS 202 T+ L KL N K WY +T Y FG L A ++ K G VG+ H Sbjct: 153 MFDTYSQAAGAAA-ALTKLGN-KSWYFVTVDYAFGHSLEKDASDVVKANGGTVVGSVRHP 210 Query: 203 LTEKEFSGYLTNAMAAKPDVLLLLNFGSQSSDTLRQAVSFGMKNNCTILMAWASGLEQFE 262 L +FS +L A A+K V+ L + G+ + ++QA SF + L+A L Sbjct: 211 LNASDFSSFLLQAQASKAQVIGLADGGTDVVNAIKQARSFAVGGKDQRLVALLVFLSDVH 270 Query: 263 TLGADICDG-VYFGAQYWHAIDSPFNKEFVKLVNDSLK-IDPNYSLAGSYICTKIILDAI 320 LG + G VY YW + + F K K I P AG Y L A Sbjct: 271 ALGLEAAQGLVYTDGFYWDYDEQ--TRAFSKRFESQFKNIKPTMVQAGVYSSVYQFLKAR 328 Query: 321 IKANSTDPKAVIAAMEGMKYEGLTGTEEIRKGDHQVIKNYYLLKGKAKAKMANKNDYADI 380 A ++D K V M + + D +VI + YL + K A+ DY + Sbjct: 329 AAAKTSDWKVVAQKMREIPIADAVMRNASIRPDGRVIHDMYLYQVKTPAESKGPWDYLKL 388 Query: 381 VS---SGKSFLPLEKTQCKL 397 +S + ++F P++ C L Sbjct: 389 LSVIPAQQAFRPMDAA-CPL 407 >gi|300312339|ref|YP_003776431.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 100 bits (249), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 87/376 (23%), Positives = 163/376 (43%), Gaps = 12/376 (3%) Query: 29 ESIAFGCPVPMSGPFAANGKFADM-GMKLALQKYG-KVLGKSTSYVTLDTEGKPATAVRK 86 +++ G MSGP+A + +++A+ +G VLGK ++ D + K A + Sbjct: 31 DAVRIGFITDMSGPYADTDGLGGLEAIRMAVADFGGTVLGKPIEVISADHQNKADIAATR 90 Query: 87 MQE-TIEKGTRFFAGGILSGEALAMGKEADKAGAVFITT-AGADEITGSDCNRATFRWSV 144 +E E+G GG+ S AL+M K + V+I AG +T +C T + Sbjct: 91 AREWADERGLDMLIGGVNSATALSMNKVMAEKKRVYINIGAGTARLTNEECTPYTVHYEY 150 Query: 145 PTFGAIEQTVRPLLEKLPNAKRWYTITPQYVFGEGLLSAAKNIFKEKGIEHVGNSYHSLT 204 T + T ++++ K W+ + Y FG L + + K G VG+ H Sbjct: 151 DTVALAKGTASAVIKQ--GGKSWFFLVADYAFGHSLANDTSAVVKASGGTVVGSIKHPPQ 208 Query: 205 EKEFSGYLTNAMAAKPDVLLLLNFGSQSSDTLRQAVSFGMKNNCTILMAWASGLEQFETL 264 + S +L A A+K +L L N G + ++++ A FG+ L+ + L Sbjct: 209 SSDLSSFLLQAQASKAQILGLANAGEDTVNSIKAAKEFGVTKTMK-LVGLLMVINDIHAL 267 Query: 265 GADICDGVYFGAQYWHAIDSPFNKEFVKLVNDSLKIDPNYSLAGSYICTKIILDAIIKAN 324 G +G+ ++ D P +++F + +K P+ A +Y T L A+ Sbjct: 268 GLANAEGLMMTDSWYWDKDEP-SRKFAERFFQKMKKMPSTHQAAAYSATTTYLKAVQAIG 326 Query: 325 STDPKAVIAAMEGMKYEGLTGTEEIRKGDHQVIKNYYLLKGKAKAKMANKNDYADIVSS- 383 + D V+A ++ + + IR D + I + YL + K+ A+ DY ++ + Sbjct: 327 TDDSTKVMAQLKKTRIDDFYNKGSIR-ADGRNIHDMYLYQVKSPAESKKPWDYLKLLETI 385 Query: 384 --GKSFLPLEKTQCKL 397 ++F + ++C L Sbjct: 386 PGEQAFTSVADSKCSL 401 >gi|300309490|ref|YP_003773582.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 99.8 bits (247), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 105/409 (25%), Positives = 175/409 (42%), Gaps = 27/409 (6%) Query: 4 RRNFLKLSALAAPGALSYHDVFAQAESIAFGCPVPMSGPFAA-NGKFADMGMKLALQKYG 62 RR L +A A GA A ++I G MSG +A +G+ +++A+ G Sbjct: 6 RRAALSATACALLGAAILPSAQAAGDTIKIGFITDMSGTYADFDGQGGVEAIRMAIADAG 65 Query: 63 KVL-GKSTSYVTLDTEGKPATAVRKMQETIEK-GTRFFAGGILSGEALAMGKEADKAGAV 120 + GK V D + K A K +E ++ G GG+ S +LAMGK A + V Sbjct: 66 GAINGKKVELVFADHQNKADIAANKAREWFDRDGVDMLVGGVNSAASLAMGKVAGEKKKV 125 Query: 121 FITT-AGADEITGSDCNRATFRWSVPTFGAIEQTVRPLLEKLPNAKRWYTITPQYVFGEG 179 FI+ AG T +CN T +++ T T + + K WY +T Y FG Sbjct: 126 FISVGAGTTRQTNEECNAYTIQYAYDTTALARGTGAAITRQ--GGKSWYFLTADYAFGTS 183 Query: 180 LLSAAKNIFKEKGIEHVGNSYHSLTEKEFSGYLTNAMAAKPDVLLLLNFGSQSSDTLRQA 239 L + K G VG + L +FS +L A ++K +L L G + ++ A Sbjct: 184 LEKDTSEVVKSNGGNVVGATRVPLATSDFSSFLLQAQSSKAQILGLAVAGGDVINAIKAA 243 Query: 240 VSFGMKNNCTILMAWASGLEQF----ETLGADICDGVYF-GAQYWHAIDSP---FNKEFV 291 FG+ N T+ +A GL F +LG + G+Y YW D N+ F+ Sbjct: 244 NEFGV--NKTMKLA---GLLIFINDTHSLGLSLTQGMYLTDGWYWDLNDQTRAWANRYFM 298 Query: 292 KLVNDSLKIDPNYSLAGSYICTKIILDAIIKANSTDPKAVIAAMEGMKYEGLTGTEEIRK 351 K +K P+ AG L A+ STD V+ + + + + + Sbjct: 299 K-----MKKMPSMFQAGDASAVGQYLKAVKAVGSTDADKVMDYLRKNRINDFFTSNGVVR 353 Query: 352 GDHQVIKNYYLLKGKAKAKMANKNDYADIVSS---GKSFLPLEKTQCKL 397 D +++ + YL++ K ++ DY +V + ++++ +++C L Sbjct: 354 PDGRMVHDMYLMEVKKPSESKRPWDYYKLVQTIPGEQAYMTKAESKCAL 402 >gi|300314071|ref|YP_003778163.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 402 Score = 89.4 bits (220), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 91/373 (24%), Positives = 162/373 (43%), Gaps = 22/373 (5%) Query: 29 ESIAFGCPVPMSGPFA-ANGKFADMGMKLALQKYGKVL-GKSTSYVTLDTEGKPATAVRK 86 + I G +SG ++ +G+ +K+A+ G + GK + D + K A K Sbjct: 29 DVIKIGFITDISGVYSDIDGQGGAEAIKMAIADMGGAINGKKIELLVADHQNKADVAASK 88 Query: 87 MQETI-EKGTRFFAGGILSGEALAMGKEADKAGAVFITT-AGADEITGSDCNRATFRWSV 144 +E ++G GG SG ALA K A + V++ AG+ +T +C+ T ++ Sbjct: 89 AREWFDQQGVDMLIGGTNSGTALASAKVAAEKKKVYMQIGAGSARLTNEECSPYTVHYAY 148 Query: 145 PTFGAIEQTVRPLLEKLPNAKRWYTITPQYVFGEGLLSAAKNIFKEKGIEHVGNSYHSLT 204 T + T ++++ K W+ +T Y FG L S N+ K G VG H L Sbjct: 149 DTVALAKGTGSAVVKQ--GGKSWFFLTADYAFGHSLESDTANVVKASGGTVVGAVKHPLG 206 Query: 205 EKEFSGYLTNAMAAKPDVLLLLNFGSQSSDTLRQAVSFGMKNNCTILMAWASGLEQF--- 261 +FS +L A ++K ++ L N G ++++ A FG+ + +GL F Sbjct: 207 ASDFSSFLLQAQSSKAQIIGLANAGGDFINSVKAANEFGITKTAKL-----AGLLVFIND 261 Query: 262 -ETLGADICDGVYF-GAQYWHAIDSPFNKEFVKLVNDSLKIDPNYSLAGSYICTKIILDA 319 +L +G+Y + YW A + + F K K P+ A Y L Sbjct: 262 VHSLTLKNTEGLYLTDSWYWDANEE--TRAFAKRYFAVRKKMPSSLQAADYSAALQYLKV 319 Query: 320 IIKANSTDPKAVIAAMEGMKYEGLTGTEEIRKGDHQVIKNYYLLKGKAKAKMANKNDYAD 379 + + + D V+AA+ K+ + + D +V+ + YL + KA+++ DY Sbjct: 320 VKEIGTDDSDKVMAALRKAKFNDMYQKNGYLRADGRVMHDMYLYQVKAQSESKYPWDYYK 379 Query: 380 IVSSGKSFLPLEK 392 +V + +P E+ Sbjct: 380 LVQT----IPAEQ 388 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,119,130 Number of extensions: 44589 Number of successful extensions: 162 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 155 Number of HSP's successfully gapped: 14 Length of query: 397 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 310 Effective length of database: 1,207,925 Effective search space: 374456750 Effective search space used: 374456750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)