BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0024.p (419 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313936|ref|YP_003778028.1| amidohydrolase [Herbaspirillum ... 840 0.0 gi|300312555|ref|YP_003776647.1| bifunctional OHCU decarboxylase... 187 2e-48 gi|300313822|ref|YP_003777914.1| N-carbamoyl-L-amino acid amidoh... 161 1e-40 >gi|300313936|ref|YP_003778028.1| amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 419 Score = 840 bits (2169), Expect = 0.0, Method: Compositional matrix adjust. Identities = 402/418 (96%), Positives = 411/418 (98%) Query: 1 MDMKTQASTLRIDGTRLWNSLMELARIGGTAKGGVCRLALTDLDKQGRDLVTSWARAEGM 60 MDM TQ STLRIDG RLW+SLMELARIG TAKGGVCRLALTDLDKQGRDLV+SWARAEGM Sbjct: 1 MDMNTQISTLRIDGARLWDSLMELARIGATAKGGVCRLALTDLDKQGRDLVSSWARAEGM 60 Query: 61 SVVVDQIGNIFMRRPGRNNALPPVMTGSHIDTQPTGGKFDGNYGVLSGIEVIRTLNQHNI 120 SVVVDQIGNIFMRRPGRNNALPPVMTGSHIDTQPTGGKFDGNYGVL+GIEV+RTLNQHNI Sbjct: 61 SVVVDQIGNIFMRRPGRNNALPPVMTGSHIDTQPTGGKFDGNYGVLAGIEVVRTLNQHNI 120 Query: 121 QTEAPIEVAVWTNEEGSRFVPVMMGSGVFCGAFTLEHAYNATDIDGKKVRDELERIGYIG 180 QTEAPIEVAVWTNEEGSRFVPVMMGSGVFCGAFTL+HAYNATD++GKKVRDELERIGYIG Sbjct: 121 QTEAPIEVAVWTNEEGSRFVPVMMGSGVFCGAFTLDHAYNATDVEGKKVRDELERIGYIG 180 Query: 181 EQVCGDHPIGAYYEAHIEQGPVLENADTVIGVVPAVLGLSWYDCTVTGFESHAGPTPMEI 240 EQVCGDHPIGAYYEAHIEQGPVLENADTVIGVVPAVLGLSWYDCTVTGFESHAGPTPMEI Sbjct: 181 EQVCGDHPIGAYYEAHIEQGPVLENADTVIGVVPAVLGLSWYDCTVTGFESHAGPTPMEI 240 Query: 241 RKDALQVSTYIMQEVVAIANRYPPYGRGTVGQVQVMPNSRNVIPGQVKFSIDLRNVSEDS 300 RKDALQVSTYIMQEVVAIA+RY PYGRGTVGQVQVMPNSRNVIPGQVKFSIDLRNVSE S Sbjct: 241 RKDALQVSTYIMQEVVAIAHRYAPYGRGTVGQVQVMPNSRNVIPGQVKFSIDLRNVSEAS 300 Query: 301 LTRMHEDLLAYLEAMRKKTGLSIELERVSYFPPCPFHPDCVDNVRAASAALGYKTMDVVS 360 LTRMHEDLLAYLEAMRKKTGLSIELERVSYFPPCPFHPDCVD VRAASAALGYKTMDVVS Sbjct: 301 LTRMHEDLLAYLEAMRKKTGLSIELERVSYFPPCPFHPDCVDKVRAASAALGYKTMDVVS 360 Query: 361 GAGHDAIYAARLAPAGMIFVPCKDGVSHNEIEDARPEHLEAGCNVLLQAMLASAGVVA 418 GAGHDAIYAARLAPAGMIFVPCKDGVSHNEIEDARPEHLEAGCNVLLQAMLASAGVV+ Sbjct: 361 GAGHDAIYAARLAPAGMIFVPCKDGVSHNEIEDARPEHLEAGCNVLLQAMLASAGVVS 418 >gi|300312555|ref|YP_003776647.1| bifunctional OHCU decarboxylase/N-carbamoyl-L-amino acid amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 587 Score = 187 bits (474), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 124/382 (32%), Positives = 193/382 (50%), Gaps = 7/382 (1%) Query: 32 KGGVCRLALTDLDKQGRDLVTSWARAEGMSVVVDQIGNIFMRRPGRNNALPPVMTGSHID 91 +G + LT+ ++ + W R GM V +D +GN+ R + ++TGSH D Sbjct: 197 EGALTCTYLTETHQRTAAQIAQWMREAGMQVHIDAVGNVVGRYQSPHPQAKTLLTGSHYD 256 Query: 92 TQPTGGKFDGNYGVLSGIEVIRTLNQHNIQTEAPIEVAVWTNEEGSRFVPVMMGSGVFCG 151 T GGK+DG G+L I V++ L++ +EV ++ EEG RF +GS G Sbjct: 257 TVRNGGKYDGREGILLPIAVVKYLHERGETLPFHLEVIGFSEEEGVRFKSTFLGSNAITG 316 Query: 152 AFTLEHAYNATDIDGKKVRDELERIGY---IGEQVCGD-HPIGAYYEAHIEQGPVLENAD 207 F + + D + +R+ + + G+ Q+ D I AY E HIEQGPVL D Sbjct: 317 HFDMA-LLDLRDKEDVTMRELIIQAGHDVAAIPQIARDPAEILAYVEVHIEQGPVLLKRD 375 Query: 208 TVIGVVPAVLGLSWYDCTVTGFESHAGPTPMEIRKDALQVSTYIMQEVVAIANRYPPYGR 267 +G+V ++ G S Y + G SHAG TPM++RKDA + I+ V ++ Sbjct: 376 LPVGIVTSIAGSSRYLVDLKGVASHAGTTPMDMRKDAAAAAAEIILYVEQRCSQDQHAAL 435 Query: 268 -GTVGQVQVMPNSRNVIPGQVKFSIDLRNVSEDSLTRMHEDLLAYLEAMRKKTGLSIELE 326 GTVGQ+QV S NVIPG + S+D+R +++ +D+LA +E + + + ++LE Sbjct: 436 VGTVGQLQVPRGSTNVIPGACQLSLDIRAATDEVRLAAVKDILAKIEEICTRRSIDLKLE 495 Query: 327 RVSYFPPCPFHPDCVDNVRAASAALGYKTMDVVSGAGHDAIYAARLAPAGMIFVPCKD-G 385 P P P + + AA+ G ++ SGAGHDA+ RL M+F C + G Sbjct: 496 ETLSAPAAPCAPWLMAQLSAATQRAGVPPFELASGAGHDAMAMVRLTDVCMLFTRCGNGG 555 Query: 386 VSHNEIEDARPEHLEAGCNVLL 407 +SHN +E + E +LL Sbjct: 556 ISHNPLETMTADDAEVSGQILL 577 >gi|300313822|ref|YP_003777914.1| N-carbamoyl-L-amino acid amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 161 bits (407), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 120/374 (32%), Positives = 178/374 (47%), Gaps = 23/374 (6%) Query: 33 GGVCRLALTDLDKQGRDLVTSWARAEGMSVVVDQIGNIFMR---RPGRNNALPPVMTGSH 89 G + R LT + + W GM V D GN+ R PG +A P +TGSH Sbjct: 30 GMLTRTYLTPQHQAAAAALARWMEEAGMRVRRDNAGNVIGRYEAAPGHESA-PSFVTGSH 88 Query: 90 IDTQPTGGKFDGNYGVLSGIEVIRTLNQHNIQTEAPIEVAVWTNEEGSRFVPVMMGSGVF 149 DT GG +DGN G++ + I ++ + PIEV + EEG RF ++GS Sbjct: 89 FDTVRNGGWYDGNLGIVLPLACIARWHRQGQRFAFPIEVIGFAEEEGVRFKATLLGSRTV 148 Query: 150 CGAFTLEHAYNATDIDGKKVRDELERIGYIGEQVCGDH----PIGAYYEAHIEQGPVLEN 205 G F + D DG +R + G + + D + A+ E HIEQGP+L N Sbjct: 149 AGTFD-QAVLENRDSDGVTMRAAIRAAGLDPDGIAADAWQPGSMAAFVEVHIEQGPLLLN 207 Query: 206 ADTVIGVVPAVLGLSWYDCTVTGFESHAGPTPMEIRKDAL----QVSTYIMQEVVAIANR 261 D +GVV A+ G S + V G HAG PM +R+DA ++ YI + Sbjct: 208 EDLPVGVVTAISGASRFMAEVHGLAGHAGTVPMHLRRDAAMAAAEIGLYIERRC-----S 262 Query: 262 YPPYGRGTVGQVQVMPNSRNVIPGQVKFSIDLRNVSEDSLTRMHE--DLLAYLEAMRKKT 319 P GT+G ++V+ + NV+PG FSID+R +ED R+ D+ A ++ + + Sbjct: 263 IKPELVGTMGLLEVVQGAANVVPGLASFSIDIR--AEDDSDRLAAVADVKAEIDRIAARR 320 Query: 320 GLSIELERVSYFPPCPFHPDCVDNVRAASAALGYKTMDVVSGAGHDAIYAARLAPAGMIF 379 + + L + P P + + AA G+ + SGAGHDA+ A LA M+F Sbjct: 321 QVQLALRQTHEAASVPCAPRLQAALAQSIAAAGWPVRHLPSGAGHDAMALAGLADVAMLF 380 Query: 380 VPCKD-GVSHNEIE 392 V C + G+SH+ E Sbjct: 381 VRCGNGGISHHPDE 394 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,404,630 Number of extensions: 62181 Number of successful extensions: 185 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 180 Number of HSP's successfully gapped: 3 Length of query: 419 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 331 Effective length of database: 1,203,190 Effective search space: 398255890 Effective search space used: 398255890 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)