BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0017.p (488 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotr... 888 0.0 gi|300310266|ref|YP_003774358.1| amidase family protein [Herbasp... 176 4e-45 gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Her... 163 3e-41 gi|300309358|ref|YP_003773450.1| amidase family protein [Herbasp... 137 2e-33 gi|300311162|ref|YP_003775254.1| amidase family protein [Herbasp... 136 4e-33 >gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotransferase subunit A [Herbaspirillum seropedicae SmR1] Length = 488 Score = 888 bits (2295), Expect = 0.0, Method: Compositional matrix adjust. Identities = 456/488 (93%), Positives = 469/488 (96%) Query: 1 MHKLTLKELSAQLQARKISATELATLFLDRIAASDLNAFLHVDRDLTLAQAKAADVRLAG 60 MHKLTLKELSAQLQARKISATELATLFLDRI ASDLNAFLHVDR+LTLAQAK AD RL+ Sbjct: 1 MHKLTLKELSAQLQARKISATELATLFLDRIDASDLNAFLHVDRELTLAQAKVADSRLSD 60 Query: 61 NDAGPLTGVPIAHKDIFVTRNWRTTAGSRMLQDYVSPFDATVVEQFNAAGTVTLGKLNCD 120 N+AG LTGVPIAHKDIFVTR WRTTAGSRML +YVSPFDATVVEQFNAAG VTLGKLNCD Sbjct: 61 NNAGQLTGVPIAHKDIFVTRGWRTTAGSRMLDNYVSPFDATVVEQFNAAGMVTLGKLNCD 120 Query: 121 EFAMGSSNENSFYGAVKNPWDKTAIPGGSSGGSAAAVAARLAPAATATDTGGSIRQPASL 180 EFAMGS NENS++G NPWDK A+PGGSSGGSAAAVAARLAPAATATDTGGSIRQPASL Sbjct: 121 EFAMGSGNENSYFGPTLNPWDKRAVPGGSSGGSAAAVAARLAPAATATDTGGSIRQPASL 180 Query: 181 CGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDSTSLQRPK 240 CGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDSTSLQRPK Sbjct: 181 CGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDSTSLQRPK 240 Query: 241 EDFTRSLNQSLQGLRIGVPKEFFGAGLAADVEAAVRAALAEFEKLGATLVEISLPKTELS 300 EDFTRSLN SLQGLRIGVPKEFFGAGLAADVEAAVRAAL+EFEKLGA LVEISLPKTELS Sbjct: 241 EDFTRSLNDSLQGLRIGVPKEFFGAGLAADVEAAVRAALSEFEKLGAQLVEISLPKTELS 300 Query: 301 IPVYYVIAPAEASSNLSRFDGVRYGHRAADYKDLADMYKKSRAEGFGDEVKRRILVGSYV 360 IPVYYVIAPAEASSNLSRFDGVRYGHRAADY +L DMY+KSRAEGFG+EVKRRILVGSYV Sbjct: 301 IPVYYVIAPAEASSNLSRFDGVRYGHRAADYGNLDDMYRKSRAEGFGEEVKRRILVGSYV 360 Query: 361 LSHGYYDAYYLQAQKIRRLIAEDFQRALAGENRQCDVIMGPVSPTVAWDLGDKTDDPVAN 420 LSHGYYDAYYLQAQKIRRLIA+DFQ ALAG NRQCDVIMGPVSPTVAWDLGDKT+DPVAN Sbjct: 361 LSHGYYDAYYLQAQKIRRLIAQDFQNALAGANRQCDVIMGPVSPTVAWDLGDKTNDPVAN 420 Query: 421 YLADIFTLSTSLAGLPGMSIPCGFGQGEKNGKRPVGLQIIGNYFDEARLLNVAHQFQQAT 480 YLADIFTLSTSLAGLPGMSIPCGFGQGEKNGKRPVGLQIIGNYFDEARLLNVAHQFQQAT Sbjct: 421 YLADIFTLSTSLAGLPGMSIPCGFGQGEKNGKRPVGLQIIGNYFDEARLLNVAHQFQQAT 480 Query: 481 DWHLREPA 488 DWHLREPA Sbjct: 481 DWHLREPA 488 >gi|300310266|ref|YP_003774358.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 176 bits (445), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 153/512 (29%), Positives = 236/512 (46%), Gaps = 54/512 (10%) Query: 6 LKELSAQLQARKISATELATLFLDRIAASDLNAFLHVD---------------------R 44 LKEL++ L + TE A+ +LD + AS +A+ +D + Sbjct: 11 LKELASSLHIQL--TTEQASQYLDLMQAS-FDAYDVIDGLPDEIPAVKYPRSAAARPAAK 67 Query: 45 DLTLAQAKAADVRLAGNDAGPLTGVPIAHKDIFVTRNWRTTAGSRMLQDYVSPFDATVVE 104 D L A A + G G L G + KD GS L+ +V +DAT+V Sbjct: 68 DNPL-NAWACKSTVKGAAEGKLAGRTVVLKDNVALAGVPMMNGSSTLEGFVPSYDATIVT 126 Query: 105 QFNAAGTVTLGKLNCDEFAMGSSNENSFYGAVKNPWDKTAIPGGSSGGSAAAVAARLAPA 164 + AG LGK C+ F + + S V NP GGSS GSAA VAA A Sbjct: 127 RLLDAGATILGKATCEHFCLSGGSHTSDPAPVHNPRRHGYGSGGSSSGSAALVAAGEADM 186 Query: 165 ATATDTGGSIRQPASLCGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNA 224 A D GGSIR P++ CG G+K T+G V G++ +++D GPI D L+L Sbjct: 187 AIGGDQGGSIRIPSAFCGTYGMKATHGLVPYTGVMPIEATVDHVGPITANVRDNALMLEV 246 Query: 225 MTGFDERDSTSLQRPKEDFTRSLNQSLQGLRIGVPKEFFGAG-LAADVEAAVRAALAEFE 283 + G D D + +T +L++ ++GL+IG+ E F G + V V+ A+A+ E Sbjct: 247 IAGADGLDPRQYAPEVQPYTAALDRGVRGLKIGILTEGFALGNMDKRVADKVQEAIAQLE 306 Query: 284 KLGATLVEISLPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAADYKDLADM------ 337 KLGA++ +S+ + L+ ++ I + + +G+ + ++K L D+ Sbjct: 307 KLGASVATVSVEEHALAGALWQPIGCEGLTMQMMHGNGMGF-----NWKGLYDLGLLQHH 361 Query: 338 -YKKSRAEGFGDEVKRRILVGSYVLSHGYYDAYYLQAQKIRRLIAEDFQRALAGENRQCD 396 + + A+ +K + VG Y L+ Y +Y +AQ I R + +ALA D Sbjct: 362 AHWREHADDLSATLKLCMFVGQYGLTR-YQGRFYAKAQNIARRARAGYDKALA----DFD 416 Query: 397 VIMGPVSPTVAWDLGDKTDDPVANYLADIF-----TLSTSLAGLPGMSIPCGFGQGEKNG 451 +++ P P +A L P+A Y+A F T + G P MSIPCG G Sbjct: 417 LLVMPTVPIIAQPL-PAAGCPLAEYVARAFEMIGNTAPLDITGHPAMSIPCGTVDG---- 471 Query: 452 KRPVGLQIIGNYFDEARLLNVAHQFQQATDWH 483 PVGL ++G ++ EA L A F+ + DW Sbjct: 472 -LPVGLMLVGKHYAEATLYQAAAAFEASMDWQ 502 >gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Herbaspirillum seropedicae SmR1] Length = 468 Score = 163 bits (413), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 165/486 (33%), Positives = 236/486 (48%), Gaps = 49/486 (10%) Query: 4 LTLKELSAQLQARKISATE-LATLFLDRIAASDLNAFLHVDRDLTLAQAKAADVRLAGND 62 +TL E+ L R+ S E + L + A DLN +L D + +QA+ D + Sbjct: 6 MTLIEVRQGLSDRRFSCLEYVDALIAETEAQWDLNCYLQRDPEQLRSQARMLDEQ---GG 62 Query: 63 AGPLTGVPIAHKDIFVTRNWRTTAGSRMLQDYVSPFDATVVEQFNAAGTVTLGKLNCDEF 122 GPL+G+PIA KD +TAG+ L++ V ATVVE AG + GK N E Sbjct: 63 HGPLSGIPIAIKDNIDVAGIASTAGTAALRNNVPQRHATVVEYLFGAGALHAGKANMHEL 122 Query: 123 AMGSSNENSFYGAVKNPWDKTAIPGGSSGGSAAAVAARLAPAATATDTGGSIRQPASLCG 182 A G +N N +G +NP+D I GGSSGG A +AARLAPAA TDTGGS+R PA+LCG Sbjct: 123 AFGITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSVRIPAALCG 182 Query: 183 VTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDSTSLQRPKED 242 V G++PT S++G++ + + D GP+A++ D LL M G RDS L Sbjct: 183 VVGLRPTSRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAG--SRDSAPL------ 234 Query: 243 FTRSLNQSLQGLRIGVPKEFFGAGLAADVEAAVRAALAEFEKLGATLVEISLPK-TELSI 301 L LR+GV + GL +VEA AAL + + G LV++ LP LS Sbjct: 235 ----LALPPSQLRLGVLRNPSWQGLEEEVEANANAALMQLREAGVELVDVDLPTLAPLSH 290 Query: 302 PVYYVIAPAEASSNLSRFDGVRYGHRAADYKDLADMYKKSRAEGFGDEVKRRI--LVGSY 359 + I E +L + +A+D + D+ K+ AE +V R I + Sbjct: 291 AAGFAIVLHEFLIDLPAY------LQASDAR---DVIKRVLAEVGSPDVARIIQATLDQP 341 Query: 360 VLSHGYYDAYYLQAQKIRRLIAEDFQRALAGENRQCDVIMGPVSPTVAWDLGDKTDDPVA 419 V H Y A L+ ++ + + D R ++ D ++ P + A LG+ DD VA Sbjct: 342 VPEHAYRLA--LEQRRTMQALYRDCFR-----SQGVDALIFPTTALTARPLGE--DDTVA 392 Query: 420 NYLADIFTLS--------TSLAGLPGMSIPCGFGQGEKNGKRPVGLQIIGNYFDEARLLN 471 + T S+AGLP +SIP G + PVGL++ G + RLL Sbjct: 393 LRGERVPTFPAFARNADGASIAGLPSISIPVGLSE----YGLPVGLELDGPEGSDRRLLQ 448 Query: 472 VAHQFQ 477 VA + Sbjct: 449 VAASLE 454 >gi|300309358|ref|YP_003773450.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 482 Score = 137 bits (346), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 147/508 (28%), Positives = 226/508 (44%), Gaps = 56/508 (11%) Query: 1 MHKLTLKE---LSAQLQARKISATELATLFLDRIAASD--LNAFLHV-DRDLTLAQAKAA 54 MH+L + LSA ++++++S E+ +L+ I + +NA + + + L +AK A Sbjct: 1 MHQLVTLDALALSAAIRSKEVSCREVMQAYLEHIEKMNPHVNAIVSLRPPEELLDEAKRA 60 Query: 55 DVRLA-GNDAGPLTGVPIAHKDIFVTRNWRTTAGSRMLQDYVSPFDATVVEQFNAAGTVT 113 D LA G G + G+P A KD+ + T+ GS + DAT V + AG + Sbjct: 61 DQLLAQGRYLGWMHGMPQAPKDLASCKGLPTSKGSPLSSMAPDQSDATCVARVRDAGAIF 120 Query: 114 LGKLNCDEFAMGSSNENSFYGAVKNPWDKTAIPGGSSGGSAAAVAARLAPAATATDTGGS 173 +GK N EF +GS++ N +G +N +D + I GGSSGG+ AA+A R+ P A +D GS Sbjct: 121 IGKTNVSEFGLGSNSYNPLFGVTRNAYDFSRIAGGSSGGAGAALAMRMLPVADGSDMMGS 180 Query: 174 IRQPASLCGVTGIKPTYGSVSR-YGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERD 232 +R PA+ V G +PT G V G F L GP+ +T D L G+D + Sbjct: 181 LRNPAAFNNVYGFRPTQGRVPYGSGGELFLQQLSTEGPMGRTVADVARLFATQAGYDPQC 240 Query: 233 STSLQRPKEDFTRSLNQSLQGLRIGVPKEFFG-AGLAADVEAAVRAALAEFEKLGATLVE 291 S L + +G RIG + G + +V AAL++F +G T VE Sbjct: 241 PLSSDTDVTGDAAQLKRDFKGARIGWLGNYDGYLPIEEEVMVLCEAALSDFADIGCT-VE 299 Query: 292 ISLP--KTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAADYKDLADMYKKSRAEGFGDE 349 P E + V NLS + F D Sbjct: 300 QCRPDYPMEKLWETWLVHRQWLVGGNLSVY--------------------------FADP 333 Query: 350 VKRRILV--GSYVLSHGYYDAYYLQAQKIRR--LIAEDFQRALAGENRQCDVIMGPVSPT 405 +R +L + + G YL A + R + D+ RA+ Q D ++ P + Sbjct: 334 ARRGLLKPEAQWEVESG----QYLTAADVFRASMARSDWYRAIHKLFEQFDYLLLPSAQM 389 Query: 406 VAWDLGDKTDDPVANYLADIF------TLSTSLAGLPGMSIPCGFGQGEKNGKRPVGLQI 459 +D+ K VA D + + +LAGLP MS+P GFG NG P+G+QI Sbjct: 390 FPFDVATKWPRNVAGREMDTYHRWMEVVIGPTLAGLPAMSVPVGFG---ANGL-PMGMQI 445 Query: 460 IGNYFDEARLLNVAHQFQQATDWHLREP 487 IG D+ +L +A+ + W + P Sbjct: 446 IGPSRDDRGVLQLAYALEGLRPWSAQAP 473 >gi|300311162|ref|YP_003775254.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 136 bits (342), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 143/481 (29%), Positives = 217/481 (45%), Gaps = 57/481 (11%) Query: 5 TLKELSAQLQARKISATELATLFLDRIA---ASDLNAFLHVDRDLTLAQAKAAD-VRLAG 60 TL L+A L A ++ +L L RIA F V + A A+A+D +R A Sbjct: 4 TLSRLAADLAAGHTTSLQLTEQALARIADPAGEGARVFTRVYAEQARAAARASDTLRAAR 63 Query: 61 NDAGPLTGVPIAHKDIFVTRNWRTTAGSRMLQDY-VSPFDATVVEQFNAAGTVTLGKLNC 119 PL GVP++ KD+F T AGS +L+ V+ A VV+ AG + +GK N Sbjct: 64 LCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGKTNM 123 Query: 120 DEFAMGSSNENSFYGAVKNPWDKTA----IPGGSSGGSAAAVAARLAPAATATDTGGSIR 175 EFA N YG +N W++ IPGGSS G+A +V +A AA +DTGGS+R Sbjct: 124 TEFAYSGLGINPHYGTPQNAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSVR 183 Query: 176 QPASLCGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDSTS 235 P++L G+TG KPT VS G++ ++ LD GP+A + + C ++ + G Sbjct: 184 IPSALNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAG-------- 235 Query: 236 LQRPKEDFTRSLNQSLQGLRIGVPKEFFGAGLAADVEAAVRAALAEFEKLGATLVEISLP 295 ED+ L L+GLR+ P G+ V A R AL + GA +VE Sbjct: 236 -----EDYVAVLAHPLRGLRLLAPTNVVLDGMDDTVGQAYRTALGKLAAAGAMIVEA--- 287 Query: 296 KTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAADYKDLADMYKKSRAEGFGDEVKRRIL 355 +P + +A A + + + HRA ++ A G+ V RIL Sbjct: 288 ----EVPAFNALAAINAKGGFTAAEAYAW-HRALIAEN---------AAGYDQRVVSRIL 333 Query: 356 VGSYVLSHGYYDAYYLQAQKIRRLIAEDFQRALAGENRQCDVIMGPVSPTVAWDLGD--K 413 G + + D + + + I ++ A+ LAG D ++ P P VA + + Sbjct: 334 RGKDMSAADLLDVLHARPRWIAQVEAQ-----LAGY----DALVMPTVPIVAPRIAELQA 384 Query: 414 TDDPVANYLADIFTLSTSLAGLPG--MSIPCGFGQGEKNGKRPVGLQIIGNYFDEARLLN 471 +DD + T + L G +S+PC G PVGL + + RLL+ Sbjct: 385 SDDAYYGANGKMLRNPTLINFLDGCALSLPC-----HAPGSAPVGLSLAACGGQDKRLLS 439 Query: 472 V 472 + Sbjct: 440 I 440 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,396,644 Number of extensions: 56717 Number of successful extensions: 174 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 156 Number of HSP's successfully gapped: 11 Length of query: 488 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 399 Effective length of database: 1,198,455 Effective search space: 478183545 Effective search space used: 478183545 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)