BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0033.0013.p (169 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313946|ref|YP_003778038.1| rod shape-determining MreD tran... 291 3e-80 >gi|300313946|ref|YP_003778038.1| rod shape-determining MreD transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 169 Score = 291 bits (744), Expect = 3e-80, Method: Compositional matrix adjust. Identities = 160/169 (94%), Positives = 167/169 (98%) Query: 1 MNNQYILLPASPLFIAFSLIVAFVLNLMPWGQMVGVPDFVALALVFWNIHQPRKVGISVA 60 MNN YILLPA+PLFIAFSL+VAF+LNLMPWGQM GVPDFVALALVFWNIHQPRKVGISVA Sbjct: 1 MNNHYILLPANPLFIAFSLVVAFLLNLMPWGQMAGVPDFVALALVFWNIHQPRKVGISVA 60 Query: 61 FMMGLLMDVNEATLLGENALAYTLLSYFAIMIHRRVLWFPLRTQALHVLPLMLLAQAVQL 120 F+MGLLMDVNEATLLGENALAYTLLSYFAIMIHRRVLWFPLRTQALHVLPL+LLAQAVQL Sbjct: 61 FLMGLLMDVNEATLLGENALAYTLLSYFAIMIHRRVLWFPLRTQALHVLPLLLLAQAVQL 120 Query: 121 VIQLLVTGKSPDWFYFSESLVSALLWPVVSVLLLAPQRRAVDRDENRPI 169 VIQLLVTGKSPDWFYFSES+VSALLWPVVS+LLLAPQRRAVDRDENRPI Sbjct: 121 VIQLLVTGKSPDWFYFSESVVSALLWPVVSLLLLAPQRRAVDRDENRPI 169 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.142 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 447,058 Number of extensions: 15250 Number of successful extensions: 67 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 67 Number of HSP's successfully gapped: 1 Length of query: 169 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 90 Effective length of database: 1,245,805 Effective search space: 112122450 Effective search space used: 112122450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 127 (53.5 bits)